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P0A9M4

- HPRT_SHIFL

UniProt

P0A9M4 - HPRT_SHIFL

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Protein
Hypoxanthine phosphoribosyltransferase
Gene
hpt, SF0122, S0124
Organism
Shigella flexneri
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Acts preferentially on hypoxanthine; has very low activity towards guanine. Inactive towards xanthine By similarity.

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei103 – 1031Proton acceptor By similarity
Binding sitei131 – 1311IMP By similarity
Binding sitei153 – 1531IMP; via carbonyl oxygen By similarity
Metal bindingi159 – 1591Magnesium By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi99 – 10810IMP By similarity
Nucleotide bindingi158 – 1592IMP By similarity

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. nucleotide binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. IMP salvage Source: UniProtKB-UniPathway
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HPRT
Gene namesi
Name:hpt
Ordered Locus Names:SF0122, S0124
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 178178Hypoxanthine phosphoribosyltransferase
PRO_0000139636Add
BLAST

Proteomic databases

PaxDbiP0A9M4.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi198214.SF0122.

Structurei

3D structure databases

ProteinModelPortaliP0A9M4.
SMRiP0A9M4. Positions 1-177.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236520.
KOiK00760.
OrthoDBiEOG693GNP.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9M4-1 [UniParc]FASTAAdd to Basket

« Hide

MKHTVEVMIP EAEIKARIAE LGRQITERYK DSGSDMVLVG LLRGSFMFMA    50
DLCREVQVSH EVDFMTASSY GSGMSTTRDV KILKDLDEDI RGKDVLIVED 100
IIDSGNTLSK VREILSLREP KSLAICTLLD KPSRREVNVP VEFIGFSIPD 150
EFVVGYGIDY AQRYRHLPYI GKVILLDE 178
Length:178
Mass (Da):20,115
Last modified:July 19, 2005 - v1
Checksum:iE1A75EB68231DC32
GO

Sequence cautioni

The sequence AAN41785.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
The sequence AAP15666.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005674 Genomic DNA. Translation: AAN41785.2. Different initiation.
AE014073 Genomic DNA. Translation: AAP15666.1. Different initiation.
RefSeqiNP_706078.4. NC_004337.2.
NP_835861.2. NC_004741.1.

Genome annotation databases

EnsemblBacteriaiAAN41785; AAN41785; SF0122.
AAP15666; AAP15666; S0124.
GeneIDi1024487.
1076555.
KEGGisfl:SF0122.
sfx:S0124.
PATRICi18701190. VBIShiFle31049_0137.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005674 Genomic DNA. Translation: AAN41785.2 . Different initiation.
AE014073 Genomic DNA. Translation: AAP15666.1 . Different initiation.
RefSeqi NP_706078.4. NC_004337.2.
NP_835861.2. NC_004741.1.

3D structure databases

ProteinModelPortali P0A9M4.
SMRi P0A9M4. Positions 1-177.
ModBasei Search...

Protein-protein interaction databases

STRINGi 198214.SF0122.

Proteomic databases

PaxDbi P0A9M4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN41785 ; AAN41785 ; SF0122 .
AAP15666 ; AAP15666 ; S0124 .
GeneIDi 1024487.
1076555.
KEGGi sfl:SF0122.
sfx:S0124.
PATRICi 18701190. VBIShiFle31049_0137.

Phylogenomic databases

eggNOGi COG0634.
HOGENOMi HOG000236520.
KOi K00760.
OrthoDBi EOG693GNP.

Enzyme and pathway databases

UniPathwayi UPA00591 ; UER00648 .

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
InterProi IPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiHPRT_SHIFL
AccessioniPrimary (citable) accession number: P0A9M4
Secondary accession number(s): P36766
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: June 11, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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