Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P0A9M2

- HPRT_ECOLI

UniProt

P0A9M2 - HPRT_ECOLI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Hypoxanthine phosphoribosyltransferase

Gene

hpt

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts preferentially on hypoxanthine; has very low activity towards guanine. Inactive towards xanthine.1 Publication

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.1 Publication

Cofactori

Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei103 – 1031Proton acceptorCurated
Binding sitei131 – 1311IMP
Binding sitei153 – 1531IMP; via carbonyl oxygen
Metal bindingi159 – 1591MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi99 – 10810IMP
Nucleotide bindingi158 – 1592IMP

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  2. hypoxanthine phosphoribosyltransferase activity Source: EcoCyc
  3. magnesium ion binding Source: EcoCyc
  4. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. adenine salvage Source: RefGenome
  2. GMP salvage Source: EcoliWiki
  3. guanine salvage Source: RefGenome
  4. hypoxanthine metabolic process Source: RefGenome
  5. IMP salvage Source: EcoliWiki
  6. purine ribonucleoside salvage Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:HYPOXANPRIBOSYLTRAN-MONOMER.
ECOL316407:JW5009-MONOMER.
MetaCyc:HYPOXANPRIBOSYLTRAN-MONOMER.
UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HPRT
Gene namesi
Name:hpt
Ordered Locus Names:b0125, JW5009
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG20098. hpt.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 178178Hypoxanthine phosphoribosyltransferasePRO_0000139632Add
BLAST

Proteomic databases

PaxDbiP0A9M2.
PRIDEiP0A9M2.

2D gel databases

SWISS-2DPAGEP0A9M2.

Expressioni

Gene expression databases

GenevestigatoriP0A9M2.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

DIPiDIP-47994N.
IntActiP0A9M2. 5 interactions.
STRINGi511145.b0125.

Structurei

Secondary structure

1
178
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95
Helixi11 – 2919
Turni30 – 323
Beta strandi36 – 427
Turni43 – 453
Helixi46 – 538
Beta strandi61 – 677
Beta strandi82 – 843
Beta strandi93 – 10412
Helixi106 – 11611
Beta strandi120 – 13011
Helixi132 – 1343
Beta strandi143 – 1475
Beta strandi153 – 1553
Beta strandi160 – 1623
Beta strandi168 – 1736

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G9SX-ray2.80A/B1-178[»]
1G9TX-ray2.80A/B1-178[»]
1GRVX-ray2.90A/B1-178[»]
ProteinModelPortaliP0A9M2.
SMRiP0A9M2. Positions 1-177.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9M2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236520.
InParanoidiP0A9M2.
KOiK00760.
OMAiVDFMTVS.
OrthoDBiEOG693GNP.
PhylomeDBiP0A9M2.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9M2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKHTVEVMIP EAEIKARIAE LGRQITERYK DSGSDMVLVG LLRGSFMFMA
60 70 80 90 100
DLCREVQVSH EVDFMTASSY GSGMSTTRDV KILKDLDEDI RGKDVLIVED
110 120 130 140 150
IIDSGNTLSK VREILSLREP KSLAICTLLD KPSRREVNVP VEFIGFSIPD
160 170
EFVVGYGIDY AQRYRHLPYI GKVILLDE
Length:178
Mass (Da):20,115
Last modified:July 19, 2005 - v1
Checksum:iE1A75EB68231DC32
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC73236.2.
AP009048 Genomic DNA. Translation: BAB96700.2.
RefSeqiNP_414667.4. NC_000913.3.
YP_488428.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73236; AAC73236; b0125.
BAB96700; BAB96700; BAB96700.
GeneIDi12932685.
946624.
KEGGiecj:Y75_p0122.
eco:b0125.
PATRICi32115353. VBIEscCol129921_0128.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC73236.2 .
AP009048 Genomic DNA. Translation: BAB96700.2 .
RefSeqi NP_414667.4. NC_000913.3.
YP_488428.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1G9S X-ray 2.80 A/B 1-178 [» ]
1G9T X-ray 2.80 A/B 1-178 [» ]
1GRV X-ray 2.90 A/B 1-178 [» ]
ProteinModelPortali P0A9M2.
SMRi P0A9M2. Positions 1-177.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47994N.
IntActi P0A9M2. 5 interactions.
STRINGi 511145.b0125.

2D gel databases

SWISS-2DPAGE P0A9M2.

Proteomic databases

PaxDbi P0A9M2.
PRIDEi P0A9M2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73236 ; AAC73236 ; b0125 .
BAB96700 ; BAB96700 ; BAB96700 .
GeneIDi 12932685.
946624.
KEGGi ecj:Y75_p0122.
eco:b0125.
PATRICi 32115353. VBIEscCol129921_0128.

Organism-specific databases

EchoBASEi EB4143.
EcoGenei EG20098. hpt.

Phylogenomic databases

eggNOGi COG0634.
HOGENOMi HOG000236520.
InParanoidi P0A9M2.
KOi K00760.
OMAi VDFMTVS.
OrthoDBi EOG693GNP.
PhylomeDBi P0A9M2.

Enzyme and pathway databases

UniPathwayi UPA00591 ; UER00648 .
BioCyci EcoCyc:HYPOXANPRIBOSYLTRAN-MONOMER.
ECOL316407:JW5009-MONOMER.
MetaCyc:HYPOXANPRIBOSYLTRAN-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0A9M2.
PROi P0A9M2.

Gene expression databases

Genevestigatori P0A9M2.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
InterProi IPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
    Fujita N., Mori H., Yura T., Ishihama A.
    Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Crystal structures of free, IMP-, and GMP-bound Escherichia coli hypoxanthine phosphoribosyltransferase."
    Guddat L.W., Vos S., Martin J.L., Keough D.T., de Jersey J.
    Protein Sci. 11:1626-1638(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) IN COMPLEXES WITH IMP; GMP AND MAGNESIUM, CATALYTIC ACTIVITY, FUNCTION, SUBUNIT, COFACTOR, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiHPRT_ECOLI
AccessioniPrimary (citable) accession number: P0A9M2
Secondary accession number(s): P36766
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3