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P0A9M2

- HPRT_ECOLI

UniProt

P0A9M2 - HPRT_ECOLI

Protein

Hypoxanthine phosphoribosyltransferase

Gene

hpt

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Acts preferentially on hypoxanthine; has very low activity towards guanine. Inactive towards xanthine.1 Publication

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.1 Publication

    Cofactori

    Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei103 – 1031Proton acceptorCurated
    Binding sitei131 – 1311IMP
    Binding sitei153 – 1531IMP; via carbonyl oxygen
    Metal bindingi159 – 1591MagnesiumBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi99 – 10810IMP
    Nucleotide bindingi158 – 1592IMP

    GO - Molecular functioni

    1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
    2. hypoxanthine phosphoribosyltransferase activity Source: EcoCyc
    3. magnesium ion binding Source: EcoCyc
    4. nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    1. adenine salvage Source: RefGenome
    2. GMP salvage Source: EcoliWiki
    3. guanine salvage Source: RefGenome
    4. hypoxanthine metabolic process Source: RefGenome
    5. IMP salvage Source: EcoliWiki
    6. purine ribonucleoside salvage Source: RefGenome

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Keywords - Ligandi

    Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:HYPOXANPRIBOSYLTRAN-MONOMER.
    ECOL316407:JW5009-MONOMER.
    MetaCyc:HYPOXANPRIBOSYLTRAN-MONOMER.
    UniPathwayiUPA00591; UER00648.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hypoxanthine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HPRT
    Gene namesi
    Name:hpt
    Ordered Locus Names:b0125, JW5009
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG20098. hpt.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: RefGenome

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 178178Hypoxanthine phosphoribosyltransferasePRO_0000139632Add
    BLAST

    Proteomic databases

    PaxDbiP0A9M2.
    PRIDEiP0A9M2.

    2D gel databases

    SWISS-2DPAGEP0A9M2.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A9M2.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    DIPiDIP-47994N.
    IntActiP0A9M2. 5 interactions.
    STRINGi511145.b0125.

    Structurei

    Secondary structure

    1
    178
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 95
    Helixi11 – 2919
    Turni30 – 323
    Beta strandi36 – 427
    Turni43 – 453
    Helixi46 – 538
    Beta strandi61 – 677
    Beta strandi82 – 843
    Beta strandi93 – 10412
    Helixi106 – 11611
    Beta strandi120 – 13011
    Helixi132 – 1343
    Beta strandi143 – 1475
    Beta strandi153 – 1553
    Beta strandi160 – 1623
    Beta strandi168 – 1736

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1G9SX-ray2.80A/B1-178[»]
    1G9TX-ray2.80A/B1-178[»]
    1GRVX-ray2.90A/B1-178[»]
    ProteinModelPortaliP0A9M2.
    SMRiP0A9M2. Positions 1-177.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A9M2.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0634.
    HOGENOMiHOG000236520.
    KOiK00760.
    OMAiVDFMTVS.
    OrthoDBiEOG693GNP.
    PhylomeDBiP0A9M2.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    InterProiIPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A9M2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKHTVEVMIP EAEIKARIAE LGRQITERYK DSGSDMVLVG LLRGSFMFMA    50
    DLCREVQVSH EVDFMTASSY GSGMSTTRDV KILKDLDEDI RGKDVLIVED 100
    IIDSGNTLSK VREILSLREP KSLAICTLLD KPSRREVNVP VEFIGFSIPD 150
    EFVVGYGIDY AQRYRHLPYI GKVILLDE 178
    Length:178
    Mass (Da):20,115
    Last modified:July 19, 2005 - v1
    Checksum:iE1A75EB68231DC32
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC73236.2.
    AP009048 Genomic DNA. Translation: BAB96700.2.
    RefSeqiNP_414667.4. NC_000913.3.
    YP_488428.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73236; AAC73236; b0125.
    BAB96700; BAB96700; BAB96700.
    GeneIDi12932685.
    946624.
    KEGGiecj:Y75_p0122.
    eco:b0125.
    PATRICi32115353. VBIEscCol129921_0128.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC73236.2 .
    AP009048 Genomic DNA. Translation: BAB96700.2 .
    RefSeqi NP_414667.4. NC_000913.3.
    YP_488428.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1G9S X-ray 2.80 A/B 1-178 [» ]
    1G9T X-ray 2.80 A/B 1-178 [» ]
    1GRV X-ray 2.90 A/B 1-178 [» ]
    ProteinModelPortali P0A9M2.
    SMRi P0A9M2. Positions 1-177.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47994N.
    IntActi P0A9M2. 5 interactions.
    STRINGi 511145.b0125.

    2D gel databases

    SWISS-2DPAGE P0A9M2.

    Proteomic databases

    PaxDbi P0A9M2.
    PRIDEi P0A9M2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73236 ; AAC73236 ; b0125 .
    BAB96700 ; BAB96700 ; BAB96700 .
    GeneIDi 12932685.
    946624.
    KEGGi ecj:Y75_p0122.
    eco:b0125.
    PATRICi 32115353. VBIEscCol129921_0128.

    Organism-specific databases

    EchoBASEi EB4143.
    EcoGenei EG20098. hpt.

    Phylogenomic databases

    eggNOGi COG0634.
    HOGENOMi HOG000236520.
    KOi K00760.
    OMAi VDFMTVS.
    OrthoDBi EOG693GNP.
    PhylomeDBi P0A9M2.

    Enzyme and pathway databases

    UniPathwayi UPA00591 ; UER00648 .
    BioCyci EcoCyc:HYPOXANPRIBOSYLTRAN-MONOMER.
    ECOL316407:JW5009-MONOMER.
    MetaCyc:HYPOXANPRIBOSYLTRAN-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A9M2.
    PROi P0A9M2.

    Gene expression databases

    Genevestigatori P0A9M2.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    InterProi IPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
      Fujita N., Mori H., Yura T., Ishihama A.
      Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Crystal structures of free, IMP-, and GMP-bound Escherichia coli hypoxanthine phosphoribosyltransferase."
      Guddat L.W., Vos S., Martin J.L., Keough D.T., de Jersey J.
      Protein Sci. 11:1626-1638(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) IN COMPLEXES WITH IMP; GMP AND MAGNESIUM, CATALYTIC ACTIVITY, FUNCTION, SUBUNIT, COFACTOR, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiHPRT_ECOLI
    AccessioniPrimary (citable) accession number: P0A9M2
    Secondary accession number(s): P36766
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3