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Protein

Hypoxanthine phosphoribosyltransferase

Gene

hpt

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts preferentially on hypoxanthine; has very low activity towards guanine. Inactive towards xanthine.1 Publication

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.1 Publication

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei103 – 1031Proton acceptorCurated
Binding sitei131 – 1311IMP
Binding sitei153 – 1531IMP; via carbonyl oxygen
Metal bindingi159 – 1591MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi99 – 10810IMP
Nucleotide bindingi158 – 1592IMP

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  2. hypoxanthine phosphoribosyltransferase activity Source: EcoCyc
  3. magnesium ion binding Source: EcoCyc
  4. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. adenine salvage Source: GO_Central
  2. GMP salvage Source: EcoliWiki
  3. guanine salvage Source: GO_Central
  4. hypoxanthine metabolic process Source: GO_Central
  5. IMP salvage Source: EcoliWiki
  6. purine ribonucleoside salvage Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:HYPOXANPRIBOSYLTRAN-MONOMER.
ECOL316407:JW5009-MONOMER.
MetaCyc:HYPOXANPRIBOSYLTRAN-MONOMER.
UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HPRT
Gene namesi
Name:hpt
Ordered Locus Names:b0125, JW5009
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG20098. hpt.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 178178Hypoxanthine phosphoribosyltransferasePRO_0000139632Add
BLAST

Proteomic databases

PaxDbiP0A9M2.
PRIDEiP0A9M2.

2D gel databases

SWISS-2DPAGEP0A9M2.

Expressioni

Gene expression databases

GenevestigatoriP0A9M2.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

DIPiDIP-47994N.
IntActiP0A9M2. 5 interactions.
STRINGi511145.b0125.

Structurei

Secondary structure

1
178
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95Combined sources
Helixi11 – 2919Combined sources
Turni30 – 323Combined sources
Beta strandi36 – 427Combined sources
Turni43 – 453Combined sources
Helixi46 – 538Combined sources
Beta strandi61 – 677Combined sources
Beta strandi82 – 843Combined sources
Beta strandi93 – 10412Combined sources
Helixi106 – 11611Combined sources
Beta strandi120 – 13011Combined sources
Helixi132 – 1343Combined sources
Beta strandi143 – 1475Combined sources
Beta strandi153 – 1553Combined sources
Beta strandi160 – 1623Combined sources
Beta strandi168 – 1736Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G9SX-ray2.80A/B1-178[»]
1G9TX-ray2.80A/B1-178[»]
1GRVX-ray2.90A/B1-178[»]
ProteinModelPortaliP0A9M2.
SMRiP0A9M2. Positions 1-177.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9M2.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236520.
InParanoidiP0A9M2.
KOiK00760.
OMAiTMDWMAV.
OrthoDBiEOG693GNP.
PhylomeDBiP0A9M2.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9M2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKHTVEVMIP EAEIKARIAE LGRQITERYK DSGSDMVLVG LLRGSFMFMA
60 70 80 90 100
DLCREVQVSH EVDFMTASSY GSGMSTTRDV KILKDLDEDI RGKDVLIVED
110 120 130 140 150
IIDSGNTLSK VREILSLREP KSLAICTLLD KPSRREVNVP VEFIGFSIPD
160 170
EFVVGYGIDY AQRYRHLPYI GKVILLDE
Length:178
Mass (Da):20,115
Last modified:July 19, 2005 - v1
Checksum:iE1A75EB68231DC32
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73236.2.
AP009048 Genomic DNA. Translation: BAB96700.2.
RefSeqiNP_414667.4. NC_000913.3.
YP_488428.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73236; AAC73236; b0125.
BAB96700; BAB96700; BAB96700.
GeneIDi12932685.
946624.
KEGGiecj:Y75_p0122.
eco:b0125.
PATRICi32115353. VBIEscCol129921_0128.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73236.2.
AP009048 Genomic DNA. Translation: BAB96700.2.
RefSeqiNP_414667.4. NC_000913.3.
YP_488428.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1G9SX-ray2.80A/B1-178[»]
1G9TX-ray2.80A/B1-178[»]
1GRVX-ray2.90A/B1-178[»]
ProteinModelPortaliP0A9M2.
SMRiP0A9M2. Positions 1-177.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47994N.
IntActiP0A9M2. 5 interactions.
STRINGi511145.b0125.

2D gel databases

SWISS-2DPAGEP0A9M2.

Proteomic databases

PaxDbiP0A9M2.
PRIDEiP0A9M2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73236; AAC73236; b0125.
BAB96700; BAB96700; BAB96700.
GeneIDi12932685.
946624.
KEGGiecj:Y75_p0122.
eco:b0125.
PATRICi32115353. VBIEscCol129921_0128.

Organism-specific databases

EchoBASEiEB4143.
EcoGeneiEG20098. hpt.

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236520.
InParanoidiP0A9M2.
KOiK00760.
OMAiTMDWMAV.
OrthoDBiEOG693GNP.
PhylomeDBiP0A9M2.

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.
BioCyciEcoCyc:HYPOXANPRIBOSYLTRAN-MONOMER.
ECOL316407:JW5009-MONOMER.
MetaCyc:HYPOXANPRIBOSYLTRAN-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A9M2.
PROiP0A9M2.

Gene expression databases

GenevestigatoriP0A9M2.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
    Fujita N., Mori H., Yura T., Ishihama A.
    Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Crystal structures of free, IMP-, and GMP-bound Escherichia coli hypoxanthine phosphoribosyltransferase."
    Guddat L.W., Vos S., Martin J.L., Keough D.T., de Jersey J.
    Protein Sci. 11:1626-1638(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) IN COMPLEXES WITH IMP; GMP AND MAGNESIUM, CATALYTIC ACTIVITY, FUNCTION, SUBUNIT, COFACTOR, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiHPRT_ECOLI
AccessioniPrimary (citable) accession number: P0A9M2
Secondary accession number(s): P36766
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: February 4, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.