P0A9L8 (P5CR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyrroline-5-carboxylate reductase
Short name=P5C reductase
Short name=P5CR
EC=1.5.1.2

Gene names

Name:	proC
Ordered Locus Names:	b0386, JW0377

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the pyrroline-5-carboxylate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NADP binding Inferred from direct assay Ref.1. Source: EcoCyc pyrroline-5-carboxylate reductase activity Inferred from direct assay PubMed 12133Ref.1. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Pyrroline-5-carboxylate reductase		PRO_0000187289

Sequences

	Sequence		Length	Mass (Da)
	P0A9L8 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: C5E2CED36BCC508D	FASTA	269	28,145
	10 20 30 40 50 60 MEKKIGFIGC GNMGKAILGG LIASGQVLPG QIWVYTPSPD KVAALHDQFG INAAESAQEV 70 80 90 100 110 120 AQIADIIFAA VKPGIMIKVL SEITSSLNKD SLVVSIAAGV TLDQLARALG HDRKIIRAMP 130 140 150 160 170 180 NTPALVNAGM TSVTPNALVT PEDTADVLNI FRCFGEAEVI AEPMIHPVVG VSGSSPAYVF 190 200 210 220 230 240 MFIEAMADAA VLGGMPRAQA YKFAAQAVMG SAKMVLETGE HPGALKDMVC SPGGTTIEAV 250 260 RVLEEKGFRA AVIEAMTKCM EKSEKLSKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Escherichia coli delta 1-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification." Deutch A.H., Smith C.J., Rushlow K.E., Kretschmer P.J. Nucleic Acids Res. 10:7701-7714(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J01665 Genomic DNA. Translation: AAA86433.1. U73857 Genomic DNA. Translation: AAB18110.1. U00096 Genomic DNA. Translation: AAC73489.1. AP009048 Genomic DNA. Translation: BAE76167.1.
PIR	RDECC. A00385.
RefSeq	NP_414920.1. NC_000913.2. YP_488679.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A9L8.
SMR	P0A9L8. Positions 1-266.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47863N.
IntAct	P0A9L8. 3 interactions.
STRING	511145.b0386.
Proteomic databases
PaxDb	P0A9L8.
PRIDE	P0A9L8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73489; AAC73489; b0386. BAE76167; BAE76167; BAE76167.
GeneID	12934539. 945034.
KEGG	ecj:Y75_p0375. eco:b0386.
PATRIC	32115913. VBIEscCol129921_0398.
Organism-specific databases
EchoBASE	EB0762.
EcoGene	EG10769. proC.
Phylogenomic databases
eggNOG	COG0345.
HOGENOM	HOG000230247.
KO	K00286.
OMA	KIVRAMP.
ProtClustDB	PRK11880.
Enzyme and pathway databases
BioCyc	EcoCyc:PYRROLINECARBREDUCT-MONOMER. ECOL316407:JW0377-MONOMER. MetaCyc:PYRROLINECARBREDUCT-MONOMER.
UniPathway	UPA00098; UER00361.
Gene expression databases
Genevestigator	P0A9L8.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR016040. NAD(P)-bd_dom. IPR004455. NADP_OxRdtase_F420. IPR000304. Pyrroline-COOH_reductase. [Graphical view]
PANTHER	PTHR11645. PTHR11645. 1 hit.
Pfam	PF03807. F420_oxidored. 1 hit. [Graphical view]
PIRSF	PIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00112. proC. 1 hit.
PROSITE	PS00521. P5CR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P5CR_ECOLI

Accession

Primary (citable) accession number: P0A9L8
Secondary accession number(s): P00373, Q2MC39

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program