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P0A9L8

- P5CR_ECOLI

UniProt

P0A9L8 - P5CR_ECOLI

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Protein

Pyrroline-5-carboxylate reductase

Gene

proC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline. Does not catalyze the reverse reaction.2 PublicationsUniRule annotation

Catalytic activityi

L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.2 PublicationsUniRule annotation

Enzyme regulationi

Inhibited by p-chloromercuribenzoate.1 Publication

Pathwayi

GO - Molecular functioni

  1. NADP binding Source: EcoCyc
  2. pyrroline-5-carboxylate reductase activity Source: EcoCyc

GO - Biological processi

  1. L-proline biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciEcoCyc:PYRROLINECARBREDUCT-MONOMER.
ECOL316407:JW0377-MONOMER.
MetaCyc:PYRROLINECARBREDUCT-MONOMER.
UniPathwayiUPA00098; UER00361.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrroline-5-carboxylate reductaseUniRule annotation (EC:1.5.1.2UniRule annotation)
Short name:
P5C reductaseUniRule annotation
Short name:
P5CRUniRule annotation
Alternative name(s):
PCA reductaseUniRule annotation
Gene namesi
Name:proCUniRule annotation
Ordered Locus Names:b0386, JW0377
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10769. proC.

Subcellular locationi

Cytoplasm 1 PublicationUniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Pyrroline-5-carboxylate reductasePRO_0000187289Add
BLAST

Proteomic databases

PaxDbiP0A9L8.
PRIDEiP0A9L8.

Expressioni

Gene expression databases

GenevestigatoriP0A9L8.

Interactioni

Protein-protein interaction databases

DIPiDIP-47863N.
IntActiP0A9L8. 4 interactions.
STRINGi511145.b0386.

Structurei

3D structure databases

ProteinModelPortaliP0A9L8.
SMRiP0A9L8. Positions 1-266.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyrroline-5-carboxylate reductase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0345.
HOGENOMiHOG000230247.
InParanoidiP0A9L8.
KOiK00286.
OMAiEMEHNGF.
OrthoDBiEOG6JB16S.
PhylomeDBiP0A9L8.

Family and domain databases

Gene3Di1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01925. P5C_reductase.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view]
PANTHERiPTHR11645. PTHR11645. 1 hit.
PfamiPF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view]
PIRSFiPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00112. proC. 1 hit.
PROSITEiPS00521. P5CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9L8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEKKIGFIGC GNMGKAILGG LIASGQVLPG QIWVYTPSPD KVAALHDQFG
60 70 80 90 100
INAAESAQEV AQIADIIFAA VKPGIMIKVL SEITSSLNKD SLVVSIAAGV
110 120 130 140 150
TLDQLARALG HDRKIIRAMP NTPALVNAGM TSVTPNALVT PEDTADVLNI
160 170 180 190 200
FRCFGEAEVI AEPMIHPVVG VSGSSPAYVF MFIEAMADAA VLGGMPRAQA
210 220 230 240 250
YKFAAQAVMG SAKMVLETGE HPGALKDMVC SPGGTTIEAV RVLEEKGFRA
260
AVIEAMTKCM EKSEKLSKS
Length:269
Mass (Da):28,145
Last modified:July 21, 1986 - v1
Checksum:iC5E2CED36BCC508D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01665 Genomic DNA. Translation: AAA86433.1.
U73857 Genomic DNA. Translation: AAB18110.1.
U00096 Genomic DNA. Translation: AAC73489.1.
AP009048 Genomic DNA. Translation: BAE76167.1.
PIRiA00385. RDECC.
RefSeqiNP_414920.1. NC_000913.3.
YP_488679.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73489; AAC73489; b0386.
BAE76167; BAE76167; BAE76167.
GeneIDi12934539.
945034.
KEGGiecj:Y75_p0375.
eco:b0386.
PATRICi32115913. VBIEscCol129921_0398.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01665 Genomic DNA. Translation: AAA86433.1 .
U73857 Genomic DNA. Translation: AAB18110.1 .
U00096 Genomic DNA. Translation: AAC73489.1 .
AP009048 Genomic DNA. Translation: BAE76167.1 .
PIRi A00385. RDECC.
RefSeqi NP_414920.1. NC_000913.3.
YP_488679.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0A9L8.
SMRi P0A9L8. Positions 1-266.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47863N.
IntActi P0A9L8. 4 interactions.
STRINGi 511145.b0386.

Proteomic databases

PaxDbi P0A9L8.
PRIDEi P0A9L8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73489 ; AAC73489 ; b0386 .
BAE76167 ; BAE76167 ; BAE76167 .
GeneIDi 12934539.
945034.
KEGGi ecj:Y75_p0375.
eco:b0386.
PATRICi 32115913. VBIEscCol129921_0398.

Organism-specific databases

EchoBASEi EB0762.
EcoGenei EG10769. proC.

Phylogenomic databases

eggNOGi COG0345.
HOGENOMi HOG000230247.
InParanoidi P0A9L8.
KOi K00286.
OMAi EMEHNGF.
OrthoDBi EOG6JB16S.
PhylomeDBi P0A9L8.

Enzyme and pathway databases

UniPathwayi UPA00098 ; UER00361 .
BioCyci EcoCyc:PYRROLINECARBREDUCT-MONOMER.
ECOL316407:JW0377-MONOMER.
MetaCyc:PYRROLINECARBREDUCT-MONOMER.

Miscellaneous databases

PROi P0A9L8.

Gene expression databases

Genevestigatori P0A9L8.

Family and domain databases

Gene3Di 1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01925. P5C_reductase.
InterProi IPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view ]
PANTHERi PTHR11645. PTHR11645. 1 hit.
Pfami PF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view ]
PIRSFi PIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMi SSF48179. SSF48179. 1 hit.
TIGRFAMsi TIGR00112. proC. 1 hit.
PROSITEi PS00521. P5CR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Escherichia coli delta 1-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification."
    Deutch A.H., Smith C.J., Rushlow K.E., Kretschmer P.J.
    Nucleic Acids Res. 10:7701-7714(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, PATHWAY, SUBCELLULAR LOCATION.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Partial purification and some properties of delta1-pyrroline-5-carboxylate reductase from Escherichia coli."
    Rossi J.J., Vender J., Berg C.M., Coleman W.H.
    J. Bacteriol. 129:108-114(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, PATHWAY.
    Strain: K12.

Entry informationi

Entry nameiP5CR_ECOLI
AccessioniPrimary (citable) accession number: P0A9L8
Secondary accession number(s): P00373, Q2MC39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Can use either NADPH or NADH, but the activity observed with NADPH is severalfold greater.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3