Reviewed, UniProtKB/Swiss-Prot P0A9L8 (P5CR_ECOLI)

Names and origin

Protein names

Recommended name:
    Pyrroline-5-carboxylate reductase
      Short name=P5C reductase
      Short name=P5CR
    EC=1.5.1.2

Gene names

Name:	proC
Ordered Locus Names:	b0386, JW0377

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-proline + NAD(P)(+) = 1-pyrroline-5-carboxylate + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the pyrroline-5-carboxylate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro pyrroline-5-carboxylate reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Pyrroline-5-carboxylate reductase		PRO_0000187289

Sequences

	Sequence		Length	Mass (Da)
	P0A9L8-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: C5E2CED36BCC508D	FASTA	269	28,145
	10 20 30 40 50 60 MEKKIGFIGC GNMGKAILGG LIASGQVLPG QIWVYTPSPD KVAALHDQFG INAAESAQEV 70 80 90 100 110 120 AQIADIIFAA VKPGIMIKVL SEITSSLNKD SLVVSIAAGV TLDQLARALG HDRKIIRAMP 130 140 150 160 170 180 NTPALVNAGM TSVTPNALVT PEDTADVLNI FRCFGEAEVI AEPMIHPVVG VSGSSPAYVF 190 200 210 220 230 240 MFIEAMADAA VLGGMPRAQA YKFAAQAVMG SAKMVLETGE HPGALKDMVC SPGGTTIEAV 250 260 RVLEEKGFRA AVIEAMTKCM EKSEKLSKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Escherichia coli delta 1-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification." Deutch A.H., Smith C.J., Rushlow K.E., Kretschmer P.J. Nucleic Acids Res. 10:7701-7714(1982) [PubMed: 6296787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J01665 Genomic DNA. Translation: AAA86433.1. U73857 Genomic DNA. Translation: AAB18110.1. U00096 Genomic DNA. Translation: AAC73489.1. AP009048 Genomic DNA. Translation: BAE76167.1.
PIR	RDECC. A00385.
RefSeq	AP_001037.1. NP_414920.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	945034.
GenomeReviews	Gene locus b0386 in contig U00096_GR. Gene locus JW0377 in contig AP009048_GR.
KEGG	ecj:JW0377. eco:b0386.
Organism-specific databases
EchoBASE	EB0762.
EcoGene	EG10769. proC.
CMR	Search...
Phylogenomic databases
HOGENOM	P0A9L8.
Enzyme and pathway databases
BioCyc	EcoCyc:PYRROLINECARBREDUCT-MON. MetaCyc:PYRROLINECARBREDUCT-MON.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd. IPR004455. NADP_OxRdtase_F420. IPR000304. P5CR. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11645. P5CR. 1 hit.
Pfam	PF03807. F420_oxidored. 1 hit. [Graphical view]
PIRSF	PIRSF000193. Pyrrol-5-carb_rd. 1 hit.
TIGRFAMs	TIGR00112. proC. 1 hit.
PROSITE	PS00521. P5CR. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P0A9L8.

Entry information

Entry name

P5CR_ECOLI

Accession

Primary (citable) accession number: P0A9L8
Secondary accession number(s): P00373, Q2MC39

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	November 25, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)