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P0A9L8 (P5CR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyrroline-5-carboxylate reductase

Short name=P5C reductase
Short name=P5CR
EC=1.5.1.2
Gene names
Name:proC
Ordered Locus Names:b0386, JW0377
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the pyrroline-5-carboxylate reductase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processL-proline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADP binding

Inferred from direct assay Ref.1. Source: EcoCyc

pyrroline-5-carboxylate reductase activity

Inferred from direct assay PubMed 12133Ref.1. Source: EcoCyc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 269269Pyrroline-5-carboxylate reductase
PRO_0000187289

Sequences

Sequence LengthMass (Da)Tools
P0A9L8 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: C5E2CED36BCC508D

FASTA26928,145
        10         20         30         40         50         60 
MEKKIGFIGC GNMGKAILGG LIASGQVLPG QIWVYTPSPD KVAALHDQFG INAAESAQEV 

        70         80         90        100        110        120 
AQIADIIFAA VKPGIMIKVL SEITSSLNKD SLVVSIAAGV TLDQLARALG HDRKIIRAMP 

       130        140        150        160        170        180 
NTPALVNAGM TSVTPNALVT PEDTADVLNI FRCFGEAEVI AEPMIHPVVG VSGSSPAYVF 

       190        200        210        220        230        240 
MFIEAMADAA VLGGMPRAQA YKFAAQAVMG SAKMVLETGE HPGALKDMVC SPGGTTIEAV 

       250        260 
RVLEEKGFRA AVIEAMTKCM EKSEKLSKS 

« Hide

References

« Hide 'large scale' references
[1]"Escherichia coli delta 1-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification."
Deutch A.H., Smith C.J., Rushlow K.E., Kretschmer P.J.
Nucleic Acids Res. 10:7701-7714(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"Sequence of minutes 4-25 of Escherichia coli."
Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J01665 Genomic DNA. Translation: AAA86433.1.
U73857 Genomic DNA. Translation: AAB18110.1.
U00096 Genomic DNA. Translation: AAC73489.1.
AP009048 Genomic DNA. Translation: BAE76167.1.
PIRRDECC. A00385.
RefSeqNP_414920.1. NC_000913.3.
YP_488679.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0A9L8.
SMRP0A9L8. Positions 1-266.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-47863N.
IntActP0A9L8. 4 interactions.
STRING511145.b0386.

Proteomic databases

PaxDbP0A9L8.
PRIDEP0A9L8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73489; AAC73489; b0386.
BAE76167; BAE76167; BAE76167.
GeneID12934539.
945034.
KEGGecj:Y75_p0375.
eco:b0386.
PATRIC32115913. VBIEscCol129921_0398.

Organism-specific databases

EchoBASEEB0762.
EcoGeneEG10769. proC.

Phylogenomic databases

eggNOGCOG0345.
HOGENOMHOG000230247.
KOK00286.
OMAKGCTIAG.
OrthoDBEOG6JB16S.
PhylomeDBP0A9L8.
ProtClustDBPRK11880.

Enzyme and pathway databases

BioCycEcoCyc:PYRROLINECARBREDUCT-MONOMER.
ECOL316407:JW0377-MONOMER.
MetaCyc:PYRROLINECARBREDUCT-MONOMER.
UniPathwayUPA00098; UER00361.

Gene expression databases

GenevestigatorP0A9L8.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view]
PANTHERPTHR11645. PTHR11645. 1 hit.
PfamPF03807. F420_oxidored. 1 hit.
[Graphical view]
PIRSFPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMSSF48179. SSF48179. 1 hit.
TIGRFAMsTIGR00112. proC. 1 hit.
PROSITEPS00521. P5CR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0A9L8.

Entry information

Entry nameP5CR_ECOLI
AccessionPrimary (citable) accession number: P0A9L8
Secondary accession number(s): P00373, Q2MC39
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene