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Protein

Pyrroline-5-carboxylate reductase

Gene

proC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline. Does not catalyze the reverse reaction.UniRule annotation2 Publications

Catalytic activityi

L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.UniRule annotation2 Publications

Enzyme regulationi

Inhibited by p-chloromercuribenzoate.1 Publication

Pathwayi

GO - Molecular functioni

  • NADP binding Source: EcoCyc
  • pyrroline-5-carboxylate reductase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Proline biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciEcoCyc:PYRROLINECARBREDUCT-MONOMER.
ECOL316407:JW0377-MONOMER.
MetaCyc:PYRROLINECARBREDUCT-MONOMER.
UniPathwayiUPA00098; UER00361.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyrroline-5-carboxylate reductaseUniRule annotation (EC:1.5.1.2UniRule annotation)
Short name:
P5C reductaseUniRule annotation
Short name:
P5CRUniRule annotation
Alternative name(s):
PCA reductaseUniRule annotation
Gene namesi
Name:proCUniRule annotation
Ordered Locus Names:b0386, JW0377
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10769. proC.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 269269Pyrroline-5-carboxylate reductasePRO_0000187289Add
BLAST

Proteomic databases

PaxDbiP0A9L8.
PRIDEiP0A9L8.

Expressioni

Gene expression databases

GenevestigatoriP0A9L8.

Interactioni

Protein-protein interaction databases

DIPiDIP-47863N.
IntActiP0A9L8. 4 interactions.
STRINGi511145.b0386.

Structurei

3D structure databases

ProteinModelPortaliP0A9L8.
SMRiP0A9L8. Positions 1-266.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyrroline-5-carboxylate reductase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0345.
HOGENOMiHOG000230247.
InParanoidiP0A9L8.
KOiK00286.
OMAiLFIEAMA.
OrthoDBiEOG6JB16S.
PhylomeDBiP0A9L8.

Family and domain databases

Gene3Di1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01925. P5C_reductase.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view]
PANTHERiPTHR11645. PTHR11645. 1 hit.
PfamiPF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view]
PIRSFiPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00112. proC. 1 hit.
PROSITEiPS00521. P5CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9L8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKKIGFIGC GNMGKAILGG LIASGQVLPG QIWVYTPSPD KVAALHDQFG
60 70 80 90 100
INAAESAQEV AQIADIIFAA VKPGIMIKVL SEITSSLNKD SLVVSIAAGV
110 120 130 140 150
TLDQLARALG HDRKIIRAMP NTPALVNAGM TSVTPNALVT PEDTADVLNI
160 170 180 190 200
FRCFGEAEVI AEPMIHPVVG VSGSSPAYVF MFIEAMADAA VLGGMPRAQA
210 220 230 240 250
YKFAAQAVMG SAKMVLETGE HPGALKDMVC SPGGTTIEAV RVLEEKGFRA
260
AVIEAMTKCM EKSEKLSKS
Length:269
Mass (Da):28,145
Last modified:July 21, 1986 - v1
Checksum:iC5E2CED36BCC508D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01665 Genomic DNA. Translation: AAA86433.1.
U73857 Genomic DNA. Translation: AAB18110.1.
U00096 Genomic DNA. Translation: AAC73489.1.
AP009048 Genomic DNA. Translation: BAE76167.1.
PIRiA00385. RDECC.
RefSeqiNP_414920.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC73489; AAC73489; b0386.
BAE76167; BAE76167; BAE76167.
GeneIDi945034.
KEGGiecj:Y75_p0375.
eco:b0386.
PATRICi32115913. VBIEscCol129921_0398.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01665 Genomic DNA. Translation: AAA86433.1.
U73857 Genomic DNA. Translation: AAB18110.1.
U00096 Genomic DNA. Translation: AAC73489.1.
AP009048 Genomic DNA. Translation: BAE76167.1.
PIRiA00385. RDECC.
RefSeqiNP_414920.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP0A9L8.
SMRiP0A9L8. Positions 1-266.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-47863N.
IntActiP0A9L8. 4 interactions.
STRINGi511145.b0386.

Proteomic databases

PaxDbiP0A9L8.
PRIDEiP0A9L8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73489; AAC73489; b0386.
BAE76167; BAE76167; BAE76167.
GeneIDi945034.
KEGGiecj:Y75_p0375.
eco:b0386.
PATRICi32115913. VBIEscCol129921_0398.

Organism-specific databases

EchoBASEiEB0762.
EcoGeneiEG10769. proC.

Phylogenomic databases

eggNOGiCOG0345.
HOGENOMiHOG000230247.
InParanoidiP0A9L8.
KOiK00286.
OMAiLFIEAMA.
OrthoDBiEOG6JB16S.
PhylomeDBiP0A9L8.

Enzyme and pathway databases

UniPathwayiUPA00098; UER00361.
BioCyciEcoCyc:PYRROLINECARBREDUCT-MONOMER.
ECOL316407:JW0377-MONOMER.
MetaCyc:PYRROLINECARBREDUCT-MONOMER.

Miscellaneous databases

PROiP0A9L8.

Gene expression databases

GenevestigatoriP0A9L8.

Family and domain databases

Gene3Di1.10.3730.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01925. P5C_reductase.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR029036. P5CR_dimer.
IPR028939. ProC_N.
IPR000304. Pyrroline-COOH_reductase.
[Graphical view]
PANTHERiPTHR11645. PTHR11645. 1 hit.
PfamiPF03807. F420_oxidored. 1 hit.
PF14748. P5CR_dimer. 1 hit.
[Graphical view]
PIRSFiPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
TIGRFAMsiTIGR00112. proC. 1 hit.
PROSITEiPS00521. P5CR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Escherichia coli delta 1-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification."
    Deutch A.H., Smith C.J., Rushlow K.E., Kretschmer P.J.
    Nucleic Acids Res. 10:7701-7714(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, PATHWAY, SUBCELLULAR LOCATION.
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Partial purification and some properties of delta1-pyrroline-5-carboxylate reductase from Escherichia coli."
    Rossi J.J., Vender J., Berg C.M., Coleman W.H.
    J. Bacteriol. 129:108-114(1977) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, PATHWAY.
    Strain: K12.

Entry informationi

Entry nameiP5CR_ECOLI
AccessioniPrimary (citable) accession number: P0A9L8
Secondary accession number(s): P00373, Q2MC39
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 27, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Can use either NADPH or NADH, but the activity observed with NADPH is severalfold greater.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.