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P0A9L8

- P5CR_ECOLI

UniProt

P0A9L8 - P5CR_ECOLI

Protein

Pyrroline-5-carboxylate reductase

Gene

proC

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of 1-pyrroline-5-carboxylate (PCA) to L-proline. Does not catalyze the reverse reaction.2 PublicationsUniRule annotation

    Catalytic activityi

    L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.2 PublicationsUniRule annotation

    Enzyme regulationi

    Inhibited by p-chloromercuribenzoate.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. NADP binding Source: EcoCyc
    2. pyrroline-5-carboxylate reductase activity Source: EcoCyc

    GO - Biological processi

    1. L-proline biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Proline biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    BioCyciEcoCyc:PYRROLINECARBREDUCT-MONOMER.
    ECOL316407:JW0377-MONOMER.
    MetaCyc:PYRROLINECARBREDUCT-MONOMER.
    UniPathwayiUPA00098; UER00361.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyrroline-5-carboxylate reductaseUniRule annotation (EC:1.5.1.2UniRule annotation)
    Short name:
    P5C reductaseUniRule annotation
    Short name:
    P5CRUniRule annotation
    Alternative name(s):
    PCA reductaseUniRule annotation
    Gene namesi
    Name:proCUniRule annotation
    Ordered Locus Names:b0386, JW0377
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10769. proC.

    Subcellular locationi

    Cytoplasm 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 269269Pyrroline-5-carboxylate reductasePRO_0000187289Add
    BLAST

    Proteomic databases

    PaxDbiP0A9L8.
    PRIDEiP0A9L8.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A9L8.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-47863N.
    IntActiP0A9L8. 4 interactions.
    STRINGi511145.b0386.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A9L8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyrroline-5-carboxylate reductase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0345.
    HOGENOMiHOG000230247.
    KOiK00286.
    OMAiEMEHNGF.
    OrthoDBiEOG6JB16S.
    PhylomeDBiP0A9L8.

    Family and domain databases

    Gene3Di1.10.3730.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPiMF_01925. P5C_reductase.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR016040. NAD(P)-bd_dom.
    IPR029036. P5CR_dimer.
    IPR028939. ProC_N.
    IPR000304. Pyrroline-COOH_reductase.
    [Graphical view]
    PANTHERiPTHR11645. PTHR11645. 1 hit.
    PfamiPF03807. F420_oxidored. 1 hit.
    PF14748. P5CR_dimer. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000193. Pyrrol-5-carb_rd. 1 hit.
    SUPFAMiSSF48179. SSF48179. 1 hit.
    TIGRFAMsiTIGR00112. proC. 1 hit.
    PROSITEiPS00521. P5CR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A9L8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKKIGFIGC GNMGKAILGG LIASGQVLPG QIWVYTPSPD KVAALHDQFG    50
    INAAESAQEV AQIADIIFAA VKPGIMIKVL SEITSSLNKD SLVVSIAAGV 100
    TLDQLARALG HDRKIIRAMP NTPALVNAGM TSVTPNALVT PEDTADVLNI 150
    FRCFGEAEVI AEPMIHPVVG VSGSSPAYVF MFIEAMADAA VLGGMPRAQA 200
    YKFAAQAVMG SAKMVLETGE HPGALKDMVC SPGGTTIEAV RVLEEKGFRA 250
    AVIEAMTKCM EKSEKLSKS 269
    Length:269
    Mass (Da):28,145
    Last modified:July 21, 1986 - v1
    Checksum:iC5E2CED36BCC508D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01665 Genomic DNA. Translation: AAA86433.1.
    U73857 Genomic DNA. Translation: AAB18110.1.
    U00096 Genomic DNA. Translation: AAC73489.1.
    AP009048 Genomic DNA. Translation: BAE76167.1.
    PIRiA00385. RDECC.
    RefSeqiNP_414920.1. NC_000913.3.
    YP_488679.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73489; AAC73489; b0386.
    BAE76167; BAE76167; BAE76167.
    GeneIDi12934539.
    945034.
    KEGGiecj:Y75_p0375.
    eco:b0386.
    PATRICi32115913. VBIEscCol129921_0398.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01665 Genomic DNA. Translation: AAA86433.1 .
    U73857 Genomic DNA. Translation: AAB18110.1 .
    U00096 Genomic DNA. Translation: AAC73489.1 .
    AP009048 Genomic DNA. Translation: BAE76167.1 .
    PIRi A00385. RDECC.
    RefSeqi NP_414920.1. NC_000913.3.
    YP_488679.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0A9L8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47863N.
    IntActi P0A9L8. 4 interactions.
    STRINGi 511145.b0386.

    Proteomic databases

    PaxDbi P0A9L8.
    PRIDEi P0A9L8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73489 ; AAC73489 ; b0386 .
    BAE76167 ; BAE76167 ; BAE76167 .
    GeneIDi 12934539.
    945034.
    KEGGi ecj:Y75_p0375.
    eco:b0386.
    PATRICi 32115913. VBIEscCol129921_0398.

    Organism-specific databases

    EchoBASEi EB0762.
    EcoGenei EG10769. proC.

    Phylogenomic databases

    eggNOGi COG0345.
    HOGENOMi HOG000230247.
    KOi K00286.
    OMAi EMEHNGF.
    OrthoDBi EOG6JB16S.
    PhylomeDBi P0A9L8.

    Enzyme and pathway databases

    UniPathwayi UPA00098 ; UER00361 .
    BioCyci EcoCyc:PYRROLINECARBREDUCT-MONOMER.
    ECOL316407:JW0377-MONOMER.
    MetaCyc:PYRROLINECARBREDUCT-MONOMER.

    Miscellaneous databases

    PROi P0A9L8.

    Gene expression databases

    Genevestigatori P0A9L8.

    Family and domain databases

    Gene3Di 1.10.3730.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPi MF_01925. P5C_reductase.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR016040. NAD(P)-bd_dom.
    IPR029036. P5CR_dimer.
    IPR028939. ProC_N.
    IPR000304. Pyrroline-COOH_reductase.
    [Graphical view ]
    PANTHERi PTHR11645. PTHR11645. 1 hit.
    Pfami PF03807. F420_oxidored. 1 hit.
    PF14748. P5CR_dimer. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000193. Pyrrol-5-carb_rd. 1 hit.
    SUPFAMi SSF48179. SSF48179. 1 hit.
    TIGRFAMsi TIGR00112. proC. 1 hit.
    PROSITEi PS00521. P5CR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Escherichia coli delta 1-pyrroline-5-carboxylate reductase: gene sequence, protein overproduction and purification."
      Deutch A.H., Smith C.J., Rushlow K.E., Kretschmer P.J.
      Nucleic Acids Res. 10:7701-7714(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, PATHWAY, SUBCELLULAR LOCATION.
    2. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Partial purification and some properties of delta1-pyrroline-5-carboxylate reductase from Escherichia coli."
      Rossi J.J., Vender J., Berg C.M., Coleman W.H.
      J. Bacteriol. 129:108-114(1977) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, PATHWAY.
      Strain: K12.

    Entry informationi

    Entry nameiP5CR_ECOLI
    AccessioniPrimary (citable) accession number: P0A9L8
    Secondary accession number(s): P00373, Q2MC39
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Can use either NADPH or NADH, but the activity observed with NADPH is severalfold greater.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3