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Protein

FKBP-type 22 kDa peptidyl-prolyl cis-trans isomerase

Gene

fklB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

Enzyme regulationi

Strongly inhibited by FK506.

GO - Molecular functioni

  • FK506 binding Source: EcoCyc
  • identical protein binding Source: IntAct
  • peptidyl-prolyl cis-trans isomerase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Rotamase

Enzyme and pathway databases

BioCyciEcoCyc:G7865-MONOMER.
MetaCyc:G7865-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
FKBP-type 22 kDa peptidyl-prolyl cis-trans isomerase (EC:5.2.1.8)
Short name:
FKBP22
Short name:
PPIase
Alternative name(s):
Rotamase
Gene namesi
Name:fklB
Synonyms:ytfC
Ordered Locus Names:b4207, JW5746
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12503. fklB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • periplasmic space Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cytoplasm, Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000753672 – 206FKBP-type 22 kDa peptidyl-prolyl cis-trans isomeraseAdd BLAST205

Proteomic databases

PaxDbiP0A9L3.
PRIDEiP0A9L3.

2D gel databases

SWISS-2DPAGEiP0A9L3.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-369295,EBI-369295

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4259639. 11 interactors.
DIPiDIP-31854N.
IntActiP0A9L3. 11 interactors.
MINTiMINT-1238607.
STRINGi316385.ECDH10B_4402.

Structurei

3D structure databases

ProteinModelPortaliP0A9L3.
SMRiP0A9L3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini120 – 206PPIase FKBP-typePROSITE-ProRule annotationAdd BLAST87

Sequence similaritiesi

Belongs to the FKBP-type PPIase family.Curated

Phylogenomic databases

eggNOGiENOG4108V1T. Bacteria.
COG0545. LUCA.
HOGENOMiHOG000154888.
InParanoidiP0A9L3.
KOiK03773.
PhylomeDBiP0A9L3.

Family and domain databases

Gene3Di1.10.287.460. 1 hit.
InterProiView protein in InterPro
IPR023566. PPIase_FKBP.
IPR001179. PPIase_FKBP_dom.
IPR000774. PPIase_FKBP_N.
IPR036944. PPIase_FKBP_N_sf.
PANTHERiPTHR43811. PTHR43811. 1 hit.
PfamiView protein in Pfam
PF00254. FKBP_C. 1 hit.
PF01346. FKBP_N. 1 hit.
PROSITEiView protein in PROSITE
PS50059. FKBP_PPIASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9L3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPTFDTIE AQASYGIGLQ VGQQLSESGL EGLLPEALVA GIADALEGKH
60 70 80 90 100
PAVPVDVVHR ALREIHERAD AVRRQRFQAM AAEGVKYLEE NAKKEGVNST
110 120 130 140 150
ESGLQFRVIN QGEGAIPART DRVRVHYTGK LIDGTVFDSS VARGEPAEFP
160 170 180 190 200
VNGVIPGWIE ALTLMPVGSK WELTIPQELA YGERGAGASI PPFSTLVFEV

ELLEIL
Length:206
Mass (Da):22,216
Last modified:January 23, 2007 - v2
Checksum:i5557F63673A101DE
GO

Sequence cautioni

The sequence AAA97103 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Mass spectrometryi

Molecular mass is 22084±1.47 Da from positions 2 - 206. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA. Translation: AAA97103.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77164.2.
AP009048 Genomic DNA. Translation: BAE78208.1.
RefSeqiNP_418628.4. NC_000913.3.
WP_000211225.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77164; AAC77164; b4207.
BAE78208; BAE78208; BAE78208.
GeneIDi948726.
KEGGiecj:JW5746.
eco:b4207.
PATRICifig|1411691.4.peg.2494.

Similar proteinsi

Entry informationi

Entry nameiFKBB_ECOLI
AccessioniPrimary (citable) accession number: P0A9L3
Secondary accession number(s): P39311
, P76801, Q2M698, Q47717
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families