Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

FKBP-type 22 kDa peptidyl-prolyl cis-trans isomerase

Gene

fklB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

Enzyme regulationi

Strongly inhibited by FK506.

GO - Molecular functioni

  • FK506 binding Source: EcoCyc
  • identical protein binding Source: IntAct
  • peptidyl-prolyl cis-trans isomerase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Rotamase

Enzyme and pathway databases

BioCyciEcoCyc:G7865-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
FKBP-type 22 kDa peptidyl-prolyl cis-trans isomerase (EC:5.2.1.8)
Short name:
FKBP22
Short name:
PPIase
Alternative name(s):
Rotamase
Gene namesi
Name:fklB
Synonyms:ytfC
Ordered Locus Names:b4207, JW5746
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12503 fklB

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • periplasmic space Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cytoplasm, Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000753672 – 206FKBP-type 22 kDa peptidyl-prolyl cis-trans isomeraseAdd BLAST205

Proteomic databases

EPDiP0A9L3
PaxDbiP0A9L3
PRIDEiP0A9L3

2D gel databases

SWISS-2DPAGEiP0A9L3

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-369295,EBI-369295

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi425963918 interactors.
DIPiDIP-31854N
IntActiP0A9L3 11 interactors.
MINTiP0A9L3
STRINGi316385.ECDH10B_4402

Structurei

3D structure databases

ProteinModelPortaliP0A9L3
SMRiP0A9L3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini120 – 206PPIase FKBP-typePROSITE-ProRule annotationAdd BLAST87

Sequence similaritiesi

Belongs to the FKBP-type PPIase family.Curated

Phylogenomic databases

eggNOGiENOG4108V1T Bacteria
COG0545 LUCA
HOGENOMiHOG000154888
InParanoidiP0A9L3
KOiK03773
OMAiHYHGTFI
PhylomeDBiP0A9L3

Family and domain databases

Gene3Di1.10.287.4601 hit
InterProiView protein in InterPro
IPR023566 PPIase_FKBP
IPR001179 PPIase_FKBP_dom
IPR000774 PPIase_FKBP_N
IPR036944 PPIase_FKBP_N_sf
PANTHERiPTHR43811 PTHR43811, 1 hit
PfamiView protein in Pfam
PF00254 FKBP_C, 1 hit
PF01346 FKBP_N, 1 hit
PROSITEiView protein in PROSITE
PS50059 FKBP_PPIASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9L3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPTFDTIE AQASYGIGLQ VGQQLSESGL EGLLPEALVA GIADALEGKH
60 70 80 90 100
PAVPVDVVHR ALREIHERAD AVRRQRFQAM AAEGVKYLEE NAKKEGVNST
110 120 130 140 150
ESGLQFRVIN QGEGAIPART DRVRVHYTGK LIDGTVFDSS VARGEPAEFP
160 170 180 190 200
VNGVIPGWIE ALTLMPVGSK WELTIPQELA YGERGAGASI PPFSTLVFEV

ELLEIL
Length:206
Mass (Da):22,216
Last modified:January 23, 2007 - v2
Checksum:i5557F63673A101DE
GO

Sequence cautioni

The sequence AAA97103 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Mass spectrometryi

Molecular mass is 22084±1.47 Da from positions 2 - 206. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97103.1 Different initiation.
U00096 Genomic DNA Translation: AAC77164.2
AP009048 Genomic DNA Translation: BAE78208.1
RefSeqiNP_418628.4, NC_000913.3
WP_000211225.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77164; AAC77164; b4207
BAE78208; BAE78208; BAE78208
GeneIDi948726
KEGGiecj:JW5746
eco:b4207
PATRICifig|1411691.4.peg.2494

Similar proteinsi

Entry informationi

Entry nameiFKBB_ECOLI
AccessioniPrimary (citable) accession number: P0A9L3
Secondary accession number(s): P39311
, P76801, Q2M698, Q47717
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome