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Reviewed, UniProtKB/Swiss-Prot P0A9J8 (PHEA_ECOLI)

Last modified June 16, 2009. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    P-protein
Including the following 2 domains:
    1- Recommended name:
            Chorismate mutase
                Short name=CM
              EC=5.4.99.5
    2- Recommended name:
            Prephenate dehydratase
                Short name=PDT
              EC=4.2.1.51
Gene names
Name: pheA
Ordered Locus Names: b2599, JW2580
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length386 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Binary interactions

With

Entry

#Exp.

IntAct

Notes

eutDP772181EBI-556114,EBI-1128886

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 386386P-protein
PRO_0000119185

Regions

Domain1 – 9292Chorismate mutase
Domain105 – 285181Prephenate dehydratase
Region286 – 386101Regulatory (Phe-binding)

Sites

Site2781Essential for prephenate dehydratase activity Potential

Secondary structure

......... 386
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A9J8-1 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 4B0960854C75A4F1

FASTA38643,111
        10         20         30         40         50         60 
MTSENPLLAL REKISALDEK LLALLAERRE LAVEVGKAKL LSHRPVRDID RERDLLERLI 

        70         80         90        100        110        120 
TLGKAHHLDA HYITRLFQLI IEDSVLTQQA LLQQHLNKIN PHSARIAFLG PKGSYSHLAA 

       130        140        150        160        170        180 
RQYAARHFEQ FIESGCAKFA DIFNQVETGQ ADYAVVPIEN TSSGAINDVY DLLQHTSLSI 

       190        200        210        220        230        240 
VGEMTLTIDH CLLVSGTTDL STINTVYSHP QPFQQCSKFL NRYPHWKIEY TESTSAAMEK 

       250        260        270        280        290        300 
VAQAKSPHVA ALGSEAGGTL YGLQVLERIE ANQRQNFTRF VVLARKAINV SDQVPAKTTL 

       310        320        330        340        350        360 
LMATGQQAGA LVEALLVLRN HNLIMTRLES RPIHGNPWEE MFYLDIQANL ESAEMQKALK 

       370        380 
ELGEITRSMK VLGCYPSENV VPVDPT 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and transcription of the phenylalanine and tyrosine operons of Escherichia coli K12."
Hudson G.S., Davidson B.E.
J. Mol. Biol. 180:1023-1051(1984) [PubMed: 6396419] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. expand/collapse author list , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"pheAo mutants of Escherichia coli have a defective pheA attenuator."
Gavini N., Davidson B.E.
J. Biol. Chem. 265:21532-21535(1990) [PubMed: 2254312] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
[6]"Nucleotide sequence of the leader region of the phenylalanine operon of Escherichia coli."
Zurawski G.R., Brown K., Killingly D., Yanofsky C.
Proc. Natl. Acad. Sci. U.S.A. 75:4271-4275(1978) [PubMed: 360214] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
[7]"Atomic structure of the buried catalytic pocket of Escherichial coli chorismate mutase."
Lee A.Y., Karplus P.A., Ganem B., Clardy J.
J. Am. Chem. Soc. 117:3627-3628(1995)
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 1-109.
[8]"Chorismate mutase-prephenate dehydratase from Escherichia coli. Study of catalytic and regulatory domains using genetically engineered proteins."
Zhang S., Pohnert G., Kongsaeree P., Wilson D.B., Clardy J., Ganem B.
J. Biol. Chem. 273:6248-6253(1998) [PubMed: 9497350] [Abstract]
Cited for: DOMAINS.

Cross-references

Sequence databases

M10431 Genomic DNA. Translation: AAA24330.1.
U00096 Genomic DNA. Translation: AAC75648.1.
AP009048 Genomic DNA. Translation: BAA16484.1.
M58024 Genomic DNA. Translation: AAA62784.1.
V00314 Genomic DNA. Translation: CAA23601.1.
PIRKMECPW. A30261.
RefSeqAP_003180.1.
NP_417090.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1ECMX-ray2.20A/B1-109[»]
ModBaseSearch...

Protein-protein interaction databases

IntActP0A9J8. 17 interactions.

Genome annotation databases

GeneID947081.
GenomeReviewsGene locus JW2580 in contig AP009048_GR.
Gene locus b2599 in contig U00096_GR.
KEGGecj:JW2580.
eco:b2599.

Organism-specific databases

EchoBASEEB0701.
EcoGeneEG10707. pheA.
CMRSearch...

Phylogenomic databases

HOGENOMP0A9J8.
OMAP0A9J8. TLYSHPQ.

Enzyme and pathway databases

BioCycEcoCyc:CHORISMUTPREPHENDEHYDRAT-MON.
MetaCyc:CHORISMUTPREPHENDEHYDRAT-MON.

Family and domain databases

InterProIPR008242. Chor_mutase/pphenate_deHydtase.
IPR002701. Chorismate_mut.
IPR010952. CM_P_1.
IPR001086. Preph_deHydtase.
IPR018528. Preph_deHydtase_CS.
[Graphical view]
PfamPF01817. CM_2. 1 hit.
PF00800. PDT. 1 hit.
[Graphical view]
PIRSFPIRSF001500. Chor_mut_pdt_Ppr. 1 hit.
TIGRFAMsTIGR01797. CM_P_1. 1 hit.
PROSITEPS51168. CHORISMATE_MUT_2. 1 hit.
PS00857. PREPHENATE_DEHYDR_1. 1 hit.
PS00858. PREPHENATE_DEHYDR_2. 1 hit.
PS51171. PREPHENATE_DEHYDR_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePHEA_ECOLI
AccessionPrimary (citable) accession number: P0A9J8
Secondary accession number(s): P07022, P78204
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents