Ribokinase - Escherichia coli (strain K12)

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P0A9J6 (RBSK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 73. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ribokinase
EC=2.7.1.15

Gene names

Name:	rbsK
Ordered Locus Names:	b3752, JW3731

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-ribose = ADP + D-ribose 5-phosphate.
Pathway	Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the carbohydrate kinase PfkB family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	D-ribose catabolic process Inferred from mutant phenotype PubMed 4889152. Source: EcoCyc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ribokinase activity Inferred from direct assay Ref.1. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ompR	P0AA16	1	EBI-546371,EBI-369514

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 309

309

Ribokinase

PRO_0000080097

Secondary structure

309

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A9J6 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 753729B41E64060E
Show »

FASTA

309

32,291

        10         20         30         40         50         60 
MQNAGSLVVL GSINADHILN LQSFPTPGET VTGNHYQVAF GGKGANQAVA AGRSGANIAF 

        70         80         90        100        110        120 
IACTGDDSIG ESVRQQLATD NIDITPVSVI KGESTGVALI FVNGEGENVI GIHAGANAAL 

       130        140        150        160        170        180 
SPALVEAQRE RIANASALLM QLESPLESVM AAAKIAHQNK TIVALNPAPA RELPDELLAL 

       190        200        210        220        230        240 
VDIITPNETE AEKLTGIRVE NDEDAAKAAQ VLHEKGIRTV LITLGSRGVW ASVNGEGQRV 

       250        260        270        280        290        300 
PGFRVQAVDT IAAGDTFNGA LITALLEEKP LPEAIRFAHA AAAIAVTRKG AQPSVPWREE 


IDAFLDRQR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ribokinase from Escherichia coli K12. Nucleotide sequence and overexpression of the rbsK gene and purification of ribokinase." Hope J.N., Bell A.W., Hermodson M.A., Groarke J.M. J. Biol. Chem. 261:7663-7668(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R. Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Purification, characterization, and crystallization of Escherichia coli ribokinase." Sigrell J.A., Cameron A.D., Jones T.A., Mowbray S.L. Protein Sci. 6:2474-2476(1997) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12.
[6]	"Structure of Escherichia coli ribokinase in complex with ribose and dinucleotide determined to 1.8-A resolution: insights into a new family of kinase structures." Sigrell J.A., Cameron A.D., Jones T.A., Mowbray S.L. Structure 6:183-193(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS). Strain: K12.
[7]	"Induced fit on sugar binding activates ribokinase." Sigrell J.A., Cameron A.D., Mowbray S.L. J. Mol. Biol. 290:1009-1018(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M13169 Genomic DNA. Translation: AAA51476.1.
L10328 Genomic DNA. Translation: AAA62105.1.
U00096 Genomic DNA. Translation: AAC76775.1.
AP009048 Genomic DNA. Translation: BAE77536.1.

PIR

KIECRB. A26305.

RefSeq

NP_418208.1. NC_000913.2.
YP_491677.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GQT	X-ray	2.34	A/B/C/D	1-309	[»]
1RK2	X-ray	2.25	A/B/C/D	1-309	[»]
1RKA	X-ray	2.30	A	1-309	[»]
1RKD	X-ray	1.84	A	1-309	[»]
1RKS	X-ray	2.40	A	1-309	[»]

ProteinModelPortal

P0A9J6.

SMR

P0A9J6. Positions 2-308.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-36178N.

IntAct

P0A9J6. 3 interactions.

MINT

MINT-1321131.

STRING

511145.b3752.

Proteomic databases

PRIDE

P0A9J6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC76775; AAC76775; b3752.
BAE77536; BAE77536; BAE77536.

GeneID

12930588.
948260.

KEGG

ecj:Y75_p3415.
eco:b3752.

PATRIC

32123003. VBIEscCol129921_3877.

Organism-specific databases

EchoBASE

EB0811.

EcoGene

EG10818. rbsK.

Phylogenomic databases

eggNOG

COG0524.

HOGENOM

HOG000235950.

K00852.

OMA

IKVTRPG.

ProtClustDB

PRK11142.

Enzyme and pathway databases

BioCyc

EcoCyc:RIBOKIN-MONOMER.
ECOL316407:JW3731-MONOMER.
MetaCyc:RIBOKIN-MONOMER.

UniPathway

UPA00916; UER00889.

Gene expression databases

Genevestigator

P0A9J6.

Family and domain databases

InterPro

IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011877. D_ribokin_bac.
IPR011611. PfkB_dom.
IPR002139. Ribokinase.
[Graphical view]

Pfam

PF00294. PfkB. 1 hit.
[Graphical view]

PRINTS

PR00990. RIBOKINASE.

TIGRFAMs

TIGR02152. D_ribokin_bact. 1 hit.

PROSITE

PS00583. PFKB_KINASES_1. False negative.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P0A9J6.

ChEMBL

CHEMBL5093.

EvolutionaryTrace

P0A9J6.

Entry information

Entry name

RBSK_ECOLI

Accession

Primary (citable) accession number: P0A9J6
Secondary accession number(s): P05054, Q2M870

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents