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P0A9J6

- RBSK_ECOLI

UniProt

P0A9J6 - RBSK_ECOLI

Protein

Ribokinase

Gene

rbsK

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (13 Aug 1987)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + D-ribose = ADP + D-ribose 5-phosphate.

    Pathwayi

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ribokinase activity Source: EcoCyc

    GO - Biological processi

    1. carbohydrate phosphorylation Source: GOC
    2. D-ribose catabolic process Source: EcoCyc

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:RIBOKIN-MONOMER.
    ECOL316407:JW3731-MONOMER.
    MetaCyc:RIBOKIN-MONOMER.
    RETL1328306-WGS:GSTH-416-MONOMER.
    RETL1328306-WGS:GSTH-5050-MONOMER.
    UniPathwayiUPA00916; UER00889.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribokinase (EC:2.7.1.15)
    Gene namesi
    Name:rbsK
    Ordered Locus Names:b3752, JW3731
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10818. rbsK.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 309309RibokinasePRO_0000080097Add
    BLAST

    Proteomic databases

    PRIDEiP0A9J6.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A9J6.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    DIPiDIP-36178N.
    IntActiP0A9J6. 3 interactions.
    MINTiMINT-1321131.
    STRINGi511145.b3752.

    Structurei

    Secondary structure

    1
    309
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 105
    Beta strandi14 – 207
    Beta strandi30 – 323
    Beta strandi36 – 416
    Helixi43 – 5412
    Beta strandi57 – 6711
    Helixi70 – 789
    Turni79 – 813
    Beta strandi87 – 904
    Beta strandi96 – 1027
    Beta strandi108 – 1136
    Helixi115 – 1195
    Helixi122 – 1265
    Helixi129 – 1346
    Beta strandi136 – 1405
    Beta strandi142 – 1443
    Helixi146 – 15813
    Beta strandi162 – 1654
    Helixi175 – 1784
    Beta strandi182 – 1843
    Helixi188 – 1958
    Helixi202 – 21413
    Beta strandi218 – 2236
    Helixi225 – 2273
    Beta strandi229 – 2335
    Beta strandi236 – 2405
    Helixi253 – 26614
    Helixi271 – 28616
    Beta strandi288 – 2914
    Helixi292 – 2943
    Helixi298 – 3069

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GQTX-ray2.34A/B/C/D1-309[»]
    1RK2X-ray2.25A/B/C/D1-309[»]
    1RKAX-ray2.30A1-309[»]
    1RKDX-ray1.84A1-309[»]
    1RKSX-ray2.40A1-309[»]
    ProteinModelPortaliP0A9J6.
    SMRiP0A9J6. Positions 2-308.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A9J6.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the carbohydrate kinase PfkB family.Curated

    Phylogenomic databases

    eggNOGiCOG0524.
    HOGENOMiHOG000235950.
    KOiK00852.
    OMAiTVIGKQY.
    OrthoDBiEOG64JFMD.
    PhylomeDBiP0A9J6.

    Family and domain databases

    Gene3Di3.40.1190.20. 1 hit.
    InterProiIPR002173. Carboh/pur_kinase_PfkB_CS.
    IPR011877. D_ribokin_bac.
    IPR011611. PfkB_dom.
    IPR002139. Ribokinase.
    IPR029056. Ribokinase-like.
    [Graphical view]
    PfamiPF00294. PfkB. 1 hit.
    [Graphical view]
    PRINTSiPR00990. RIBOKINASE.
    SUPFAMiSSF53613. SSF53613. 1 hit.
    TIGRFAMsiTIGR02152. D_ribokin_bact. 1 hit.
    PROSITEiPS00584. PFKB_KINASES_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A9J6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQNAGSLVVL GSINADHILN LQSFPTPGET VTGNHYQVAF GGKGANQAVA    50
    AGRSGANIAF IACTGDDSIG ESVRQQLATD NIDITPVSVI KGESTGVALI 100
    FVNGEGENVI GIHAGANAAL SPALVEAQRE RIANASALLM QLESPLESVM 150
    AAAKIAHQNK TIVALNPAPA RELPDELLAL VDIITPNETE AEKLTGIRVE 200
    NDEDAAKAAQ VLHEKGIRTV LITLGSRGVW ASVNGEGQRV PGFRVQAVDT 250
    IAAGDTFNGA LITALLEEKP LPEAIRFAHA AAAIAVTRKG AQPSVPWREE 300
    IDAFLDRQR 309
    Length:309
    Mass (Da):32,291
    Last modified:August 13, 1987 - v1
    Checksum:i753729B41E64060E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13169 Genomic DNA. Translation: AAA51476.1.
    L10328 Genomic DNA. Translation: AAA62105.1.
    U00096 Genomic DNA. Translation: AAC76775.1.
    AP009048 Genomic DNA. Translation: BAE77536.1.
    PIRiA26305. KIECRB.
    RefSeqiNP_418208.1. NC_000913.3.
    YP_491677.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76775; AAC76775; b3752.
    BAE77536; BAE77536; BAE77536.
    GeneIDi12930588.
    948260.
    KEGGiecj:Y75_p3415.
    eco:b3752.
    PATRICi32123003. VBIEscCol129921_3877.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13169 Genomic DNA. Translation: AAA51476.1 .
    L10328 Genomic DNA. Translation: AAA62105.1 .
    U00096 Genomic DNA. Translation: AAC76775.1 .
    AP009048 Genomic DNA. Translation: BAE77536.1 .
    PIRi A26305. KIECRB.
    RefSeqi NP_418208.1. NC_000913.3.
    YP_491677.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GQT X-ray 2.34 A/B/C/D 1-309 [» ]
    1RK2 X-ray 2.25 A/B/C/D 1-309 [» ]
    1RKA X-ray 2.30 A 1-309 [» ]
    1RKD X-ray 1.84 A 1-309 [» ]
    1RKS X-ray 2.40 A 1-309 [» ]
    ProteinModelPortali P0A9J6.
    SMRi P0A9J6. Positions 2-308.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-36178N.
    IntActi P0A9J6. 3 interactions.
    MINTi MINT-1321131.
    STRINGi 511145.b3752.

    Chemistry

    BindingDBi P0A9J6.
    ChEMBLi CHEMBL5093.

    Proteomic databases

    PRIDEi P0A9J6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76775 ; AAC76775 ; b3752 .
    BAE77536 ; BAE77536 ; BAE77536 .
    GeneIDi 12930588.
    948260.
    KEGGi ecj:Y75_p3415.
    eco:b3752.
    PATRICi 32123003. VBIEscCol129921_3877.

    Organism-specific databases

    EchoBASEi EB0811.
    EcoGenei EG10818. rbsK.

    Phylogenomic databases

    eggNOGi COG0524.
    HOGENOMi HOG000235950.
    KOi K00852.
    OMAi TVIGKQY.
    OrthoDBi EOG64JFMD.
    PhylomeDBi P0A9J6.

    Enzyme and pathway databases

    UniPathwayi UPA00916 ; UER00889 .
    BioCyci EcoCyc:RIBOKIN-MONOMER.
    ECOL316407:JW3731-MONOMER.
    MetaCyc:RIBOKIN-MONOMER.
    RETL1328306-WGS:GSTH-416-MONOMER.
    RETL1328306-WGS:GSTH-5050-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A9J6.
    PROi P0A9J6.

    Gene expression databases

    Genevestigatori P0A9J6.

    Family and domain databases

    Gene3Di 3.40.1190.20. 1 hit.
    InterProi IPR002173. Carboh/pur_kinase_PfkB_CS.
    IPR011877. D_ribokin_bac.
    IPR011611. PfkB_dom.
    IPR002139. Ribokinase.
    IPR029056. Ribokinase-like.
    [Graphical view ]
    Pfami PF00294. PfkB. 1 hit.
    [Graphical view ]
    PRINTSi PR00990. RIBOKINASE.
    SUPFAMi SSF53613. SSF53613. 1 hit.
    TIGRFAMsi TIGR02152. D_ribokin_bact. 1 hit.
    PROSITEi PS00584. PFKB_KINASES_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ribokinase from Escherichia coli K12. Nucleotide sequence and overexpression of the rbsK gene and purification of ribokinase."
      Hope J.N., Bell A.W., Hermodson M.A., Groarke J.M.
      J. Biol. Chem. 261:7663-7668(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
      Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
      Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Purification, characterization, and crystallization of Escherichia coli ribokinase."
      Sigrell J.A., Cameron A.D., Jones T.A., Mowbray S.L.
      Protein Sci. 6:2474-2476(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: K12.
    6. "Structure of Escherichia coli ribokinase in complex with ribose and dinucleotide determined to 1.8-A resolution: insights into a new family of kinase structures."
      Sigrell J.A., Cameron A.D., Jones T.A., Mowbray S.L.
      Structure 6:183-193(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS).
      Strain: K12.
    7. "Induced fit on sugar binding activates ribokinase."
      Sigrell J.A., Cameron A.D., Mowbray S.L.
      J. Mol. Biol. 290:1009-1018(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).

    Entry informationi

    Entry nameiRBSK_ECOLI
    AccessioniPrimary (citable) accession number: P0A9J6
    Secondary accession number(s): P05054, Q2M870
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 1987
    Last sequence update: August 13, 1987
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3