P0A9J6 (RBSK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribokinase
EC=2.7.1.15

Gene names

Name:	rbsK
Ordered Locus Names:	b3752, JW3731

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + D-ribose = ADP + D-ribose 5-phosphate.
Pathway	Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	D-ribose catabolic process Inferred from mutant phenotype. Source: EcoCyc
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ribokinase activity Inferred from direct assay Ref.1. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ompR	P0AA16	1	EBI-546371,EBI-369514

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 309

309

Ribokinase

PRO_0000080097

Secondary structure

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309

Helix Strand Turn

Details...

Beta strand

6 – 10

5

Beta strand

14 – 20

7

Beta strand

30 – 32

3

Beta strand

34 – 41

8

Helix

43 – 54

12

Beta strand

57 – 67

11

Helix

68 – 79

12

Beta strand

87 – 90

4

Beta strand

96 – 102

7

Beta strand

108 – 113

6

Helix

115 – 119

5

Helix

122 – 126

5

Helix

129 – 133

5

Beta strand

136 – 140

5

Beta strand

142 – 144

3

Helix

146 – 158

13

Beta strand

162 – 165

4

Helix

175 – 178

4

Beta strand

182 – 184

3

Helix

188 – 195

8

Helix

202 – 214

13

Beta strand

218 – 223

6

Helix

225 – 227

3

Beta strand

229 – 233

5

Beta strand

236 – 240

5

Helix

253 – 265

13

Turn

266 – 268

3

Helix

271 – 285

15

Beta strand

288 – 291

4

Helix

292 – 294

3

Helix

298 – 306

9

Sequences

	Sequence		Length	Mass (Da)
	P0A9J6 [UniParc]. Last modified August 13, 1987. Version 1. Checksum: 753729B41E64060E	FASTA	309	32,291
	10 20 30 40 50 60 MQNAGSLVVL GSINADHILN LQSFPTPGET VTGNHYQVAF GGKGANQAVA AGRSGANIAF 70 80 90 100 110 120 IACTGDDSIG ESVRQQLATD NIDITPVSVI KGESTGVALI FVNGEGENVI GIHAGANAAL 130 140 150 160 170 180 SPALVEAQRE RIANASALLM QLESPLESVM AAAKIAHQNK TIVALNPAPA RELPDELLAL 190 200 210 220 230 240 VDIITPNETE AEKLTGIRVE NDEDAAKAAQ VLHEKGIRTV LITLGSRGVW ASVNGEGQRV 250 260 270 280 290 300 PGFRVQAVDT IAAGDTFNGA LITALLEEKP LPEAIRFAHA AAAIAVTRKG AQPSVPWREE IDAFLDRQR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ribokinase from Escherichia coli K12. Nucleotide sequence and overexpression of the rbsK gene and purification of ribokinase." Hope J.N., Bell A.W., Hermodson M.A., Groarke J.M. J. Biol. Chem. 261:7663-7668(1986) [PubMed: 3011794] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R. Genomics 16:551-561(1993) [PubMed: 7686882] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Purification, characterization, and crystallization of Escherichia coli ribokinase." Sigrell J.A., Cameron A.D., Jones T.A., Mowbray S.L. Protein Sci. 6:2474-2476(1997) [PubMed: 9385653] [Abstract] Cited for: CHARACTERIZATION. Strain: K12.
[6]	"Structure of Escherichia coli ribokinase in complex with ribose and dinucleotide determined to 1.8-A resolution: insights into a new family of kinase structures." Sigrell J.A., Cameron A.D., Jones T.A., Mowbray S.L. Structure 6:183-193(1998) [PubMed: 9519409] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS). Strain: K12.
[7]	"Induced fit on sugar binding activates ribokinase." Sigrell J.A., Cameron A.D., Mowbray S.L. J. Mol. Biol. 290:1009-1018(1999) [PubMed: 10438599] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M13169 Genomic DNA. Translation: AAA51476.1.
L10328 Genomic DNA. Translation: AAA62105.1.
U00096 Genomic DNA. Translation: AAC76775.1.
AP009048 Genomic DNA. Translation: BAE77536.1.

PIR

KIECRB. A26305.

RefSeq

NP_418208.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GQT	X-ray	2.34	A/B/C/D	1-309	[»]
1RK2	X-ray	2.25	A/B/C/D	1-309	[»]
1RKA	X-ray	2.30	A	1-309	[»]
1RKD	X-ray	1.84	A	1-309	[»]
1RKS	X-ray	2.40	A	1-309	[»]

ProteinModelPortal

P0A9J6.

SMR

P0A9J6. Positions 2-308.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-36178N.

IntAct

P0A9J6. 3 interactions.

MINT

MINT-1321131.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000001888; EBESCP00000001888; EBESCG00000001550.
EBESCT00000017434; EBESCP00000016725; EBESCG00000016490.

GeneID

948260.

GenomeReviews

Gene locus JW3731 in contig AP009048_GR.
Gene locus b3752 in contig U00096_GR.

KEGG

ecj:JW3731.
eco:b3752.

PATRIC

32123003. VBIEscCol129921_3877.

Organism-specific databases

EchoBASE

EB0811.

EcoGene

EG10818. rbsK.

Phylogenomic databases

eggNOG

COG0524.

GeneTree

EBGT00070000031691.

HOGENOM

HBG697973.

OMA

INTEAVM.

PhylomeDB

P0A9J6.

ProtClustDB

PRK11142.

Enzyme and pathway databases

BioCyc

EcoCyc:RIBOKIN-MONOMER.
MetaCyc:RIBOKIN-MONOMER.

Gene expression databases

Genevestigator

P0A9J6.

Family and domain databases

InterPro

IPR011611. Carb/pur_kinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011877. D_ribokin_bac.
IPR002139. Ribokinase.
[Graphical view]

KO

K00852.

Pfam

PF00294. PfkB. 1 hit.
[Graphical view]

PRINTS

PR00990. RIBOKINASE.

TIGRFAMs

TIGR02152. D_ribokin_bact. 1 hit.

PROSITE

PS00583. PFKB_KINASES_1. False negative.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RBSK_ECOLI

Accession

Primary (citable) accession number: P0A9J6
Secondary accession number(s): P05054, Q2M870

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 1987
Last sequence update:	August 13, 1987
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program