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Protein

Ribokinase

Gene

rbsK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + D-ribose = ADP + D-ribose 5-phosphate.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribokinase activity Source: EcoCyc

GO - Biological processi

  1. carbohydrate phosphorylation Source: GOC
  2. D-ribose catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:RIBOKIN-MONOMER.
ECOL316407:JW3731-MONOMER.
MetaCyc:RIBOKIN-MONOMER.
RETL1328306-WGS:GSTH-416-MONOMER.
RETL1328306-WGS:GSTH-5050-MONOMER.
BRENDAi2.7.1.15. 2026.
UniPathwayiUPA00916; UER00889.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribokinase (EC:2.7.1.15)
Gene namesi
Name:rbsK
Ordered Locus Names:b3752, JW3731
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10818. rbsK.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309RibokinasePRO_0000080097Add
BLAST

Proteomic databases

PRIDEiP0A9J6.

Expressioni

Gene expression databases

GenevestigatoriP0A9J6.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

DIPiDIP-36178N.
IntActiP0A9J6. 3 interactions.
MINTiMINT-1321131.
STRINGi511145.b3752.

Structurei

Secondary structure

1
309
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 105Combined sources
Beta strandi14 – 207Combined sources
Beta strandi30 – 323Combined sources
Beta strandi36 – 416Combined sources
Helixi43 – 5412Combined sources
Beta strandi57 – 6711Combined sources
Helixi70 – 789Combined sources
Turni79 – 813Combined sources
Beta strandi87 – 904Combined sources
Beta strandi96 – 1027Combined sources
Beta strandi108 – 1136Combined sources
Helixi115 – 1195Combined sources
Helixi122 – 1265Combined sources
Helixi129 – 1346Combined sources
Beta strandi136 – 1405Combined sources
Beta strandi142 – 1443Combined sources
Helixi146 – 15813Combined sources
Beta strandi162 – 1654Combined sources
Helixi175 – 1784Combined sources
Beta strandi182 – 1843Combined sources
Helixi188 – 1958Combined sources
Helixi202 – 21413Combined sources
Beta strandi218 – 2236Combined sources
Helixi225 – 2273Combined sources
Beta strandi229 – 2335Combined sources
Beta strandi236 – 2405Combined sources
Helixi253 – 26614Combined sources
Helixi271 – 28616Combined sources
Beta strandi288 – 2914Combined sources
Helixi292 – 2943Combined sources
Helixi298 – 3069Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GQTX-ray2.34A/B/C/D1-309[»]
1RK2X-ray2.25A/B/C/D1-309[»]
1RKAX-ray2.30A1-309[»]
1RKDX-ray1.84A1-309[»]
1RKSX-ray2.40A1-309[»]
ProteinModelPortaliP0A9J6.
SMRiP0A9J6. Positions 2-308.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9J6.

Family & Domainsi

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family.Curated

Phylogenomic databases

eggNOGiCOG0524.
HOGENOMiHOG000235950.
InParanoidiP0A9J6.
KOiK00852.
OMAiNGALMTA.
OrthoDBiEOG64JFMD.
PhylomeDBiP0A9J6.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiIPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011877. D_ribokin_bac.
IPR011611. PfkB_dom.
IPR002139. Ribokinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF00294. PfkB. 1 hit.
[Graphical view]
PRINTSiPR00990. RIBOKINASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR02152. D_ribokin_bact. 1 hit.
PROSITEiPS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9J6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNAGSLVVL GSINADHILN LQSFPTPGET VTGNHYQVAF GGKGANQAVA
60 70 80 90 100
AGRSGANIAF IACTGDDSIG ESVRQQLATD NIDITPVSVI KGESTGVALI
110 120 130 140 150
FVNGEGENVI GIHAGANAAL SPALVEAQRE RIANASALLM QLESPLESVM
160 170 180 190 200
AAAKIAHQNK TIVALNPAPA RELPDELLAL VDIITPNETE AEKLTGIRVE
210 220 230 240 250
NDEDAAKAAQ VLHEKGIRTV LITLGSRGVW ASVNGEGQRV PGFRVQAVDT
260 270 280 290 300
IAAGDTFNGA LITALLEEKP LPEAIRFAHA AAAIAVTRKG AQPSVPWREE

IDAFLDRQR
Length:309
Mass (Da):32,291
Last modified:August 13, 1987 - v1
Checksum:i753729B41E64060E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13169 Genomic DNA. Translation: AAA51476.1.
L10328 Genomic DNA. Translation: AAA62105.1.
U00096 Genomic DNA. Translation: AAC76775.1.
AP009048 Genomic DNA. Translation: BAE77536.1.
PIRiA26305. KIECRB.
RefSeqiNP_418208.1. NC_000913.3.
YP_491677.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76775; AAC76775; b3752.
BAE77536; BAE77536; BAE77536.
GeneIDi12930588.
948260.
KEGGiecj:Y75_p3415.
eco:b3752.
PATRICi32123003. VBIEscCol129921_3877.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13169 Genomic DNA. Translation: AAA51476.1.
L10328 Genomic DNA. Translation: AAA62105.1.
U00096 Genomic DNA. Translation: AAC76775.1.
AP009048 Genomic DNA. Translation: BAE77536.1.
PIRiA26305. KIECRB.
RefSeqiNP_418208.1. NC_000913.3.
YP_491677.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GQTX-ray2.34A/B/C/D1-309[»]
1RK2X-ray2.25A/B/C/D1-309[»]
1RKAX-ray2.30A1-309[»]
1RKDX-ray1.84A1-309[»]
1RKSX-ray2.40A1-309[»]
ProteinModelPortaliP0A9J6.
SMRiP0A9J6. Positions 2-308.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-36178N.
IntActiP0A9J6. 3 interactions.
MINTiMINT-1321131.
STRINGi511145.b3752.

Chemistry

BindingDBiP0A9J6.
ChEMBLiCHEMBL5093.

Proteomic databases

PRIDEiP0A9J6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76775; AAC76775; b3752.
BAE77536; BAE77536; BAE77536.
GeneIDi12930588.
948260.
KEGGiecj:Y75_p3415.
eco:b3752.
PATRICi32123003. VBIEscCol129921_3877.

Organism-specific databases

EchoBASEiEB0811.
EcoGeneiEG10818. rbsK.

Phylogenomic databases

eggNOGiCOG0524.
HOGENOMiHOG000235950.
InParanoidiP0A9J6.
KOiK00852.
OMAiNGALMTA.
OrthoDBiEOG64JFMD.
PhylomeDBiP0A9J6.

Enzyme and pathway databases

UniPathwayiUPA00916; UER00889.
BioCyciEcoCyc:RIBOKIN-MONOMER.
ECOL316407:JW3731-MONOMER.
MetaCyc:RIBOKIN-MONOMER.
RETL1328306-WGS:GSTH-416-MONOMER.
RETL1328306-WGS:GSTH-5050-MONOMER.
BRENDAi2.7.1.15. 2026.

Miscellaneous databases

EvolutionaryTraceiP0A9J6.
PROiP0A9J6.

Gene expression databases

GenevestigatoriP0A9J6.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiIPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011877. D_ribokin_bac.
IPR011611. PfkB_dom.
IPR002139. Ribokinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF00294. PfkB. 1 hit.
[Graphical view]
PRINTSiPR00990. RIBOKINASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR02152. D_ribokin_bact. 1 hit.
PROSITEiPS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Ribokinase from Escherichia coli K12. Nucleotide sequence and overexpression of the rbsK gene and purification of ribokinase."
    Hope J.N., Bell A.W., Hermodson M.A., Groarke J.M.
    J. Biol. Chem. 261:7663-7668(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
    Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
    Genomics 16:551-561(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Purification, characterization, and crystallization of Escherichia coli ribokinase."
    Sigrell J.A., Cameron A.D., Jones T.A., Mowbray S.L.
    Protein Sci. 6:2474-2476(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: K12.
  6. "Structure of Escherichia coli ribokinase in complex with ribose and dinucleotide determined to 1.8-A resolution: insights into a new family of kinase structures."
    Sigrell J.A., Cameron A.D., Jones T.A., Mowbray S.L.
    Structure 6:183-193(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS).
    Strain: K12.
  7. "Induced fit on sugar binding activates ribokinase."
    Sigrell J.A., Cameron A.D., Mowbray S.L.
    J. Mol. Biol. 290:1009-1018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).

Entry informationi

Entry nameiRBSK_ECOLI
AccessioniPrimary (citable) accession number: P0A9J6
Secondary accession number(s): P05054, Q2M870
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: April 1, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.