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P0A9J6 (RBSK_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribokinase

EC=2.7.1.15
Gene names
Name:rbsK
Ordered Locus Names:b3752, JW3731
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length309 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + D-ribose = ADP + D-ribose 5-phosphate.

Pathway

Carbohydrate metabolism; D-ribose degradation; D-ribose 5-phosphate from beta-D-ribopyranose: step 2/2.

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the carbohydrate kinase PfkB family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processD-ribose catabolic process

Inferred from mutant phenotype PubMed 4889152. Source: EcoCyc

carbohydrate phosphorylation

Inferred from direct assay Ref.1. Source: GOC

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ribokinase activity

Inferred from direct assay Ref.1. Source: EcoCyc

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 309309Ribokinase
PRO_0000080097

Secondary structure

............................................................. 309
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A9J6 [UniParc].

Last modified August 13, 1987. Version 1.
Checksum: 753729B41E64060E

FASTA30932,291
        10         20         30         40         50         60 
MQNAGSLVVL GSINADHILN LQSFPTPGET VTGNHYQVAF GGKGANQAVA AGRSGANIAF 

        70         80         90        100        110        120 
IACTGDDSIG ESVRQQLATD NIDITPVSVI KGESTGVALI FVNGEGENVI GIHAGANAAL 

       130        140        150        160        170        180 
SPALVEAQRE RIANASALLM QLESPLESVM AAAKIAHQNK TIVALNPAPA RELPDELLAL 

       190        200        210        220        230        240 
VDIITPNETE AEKLTGIRVE NDEDAAKAAQ VLHEKGIRTV LITLGSRGVW ASVNGEGQRV 

       250        260        270        280        290        300 
PGFRVQAVDT IAAGDTFNGA LITALLEEKP LPEAIRFAHA AAAIAVTRKG AQPSVPWREE 


IDAFLDRQR 

« Hide

References

« Hide 'large scale' references
[1]"Ribokinase from Escherichia coli K12. Nucleotide sequence and overexpression of the rbsK gene and purification of ribokinase."
Hope J.N., Bell A.W., Hermodson M.A., Groarke J.M.
J. Biol. Chem. 261:7663-7668(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Purification, characterization, and crystallization of Escherichia coli ribokinase."
Sigrell J.A., Cameron A.D., Jones T.A., Mowbray S.L.
Protein Sci. 6:2474-2476(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: K12.
[6]"Structure of Escherichia coli ribokinase in complex with ribose and dinucleotide determined to 1.8-A resolution: insights into a new family of kinase structures."
Sigrell J.A., Cameron A.D., Jones T.A., Mowbray S.L.
Structure 6:183-193(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS).
Strain: K12.
[7]"Induced fit on sugar binding activates ribokinase."
Sigrell J.A., Cameron A.D., Mowbray S.L.
J. Mol. Biol. 290:1009-1018(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M13169 Genomic DNA. Translation: AAA51476.1.
L10328 Genomic DNA. Translation: AAA62105.1.
U00096 Genomic DNA. Translation: AAC76775.1.
AP009048 Genomic DNA. Translation: BAE77536.1.
PIRKIECRB. A26305.
RefSeqNP_418208.1. NC_000913.3.
YP_491677.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GQTX-ray2.34A/B/C/D1-309[»]
1RK2X-ray2.25A/B/C/D1-309[»]
1RKAX-ray2.30A1-309[»]
1RKDX-ray1.84A1-309[»]
1RKSX-ray2.40A1-309[»]
ProteinModelPortalP0A9J6.
SMRP0A9J6. Positions 2-308.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-36178N.
IntActP0A9J6. 3 interactions.
MINTMINT-1321131.
STRING511145.b3752.

Chemistry

BindingDBP0A9J6.
ChEMBLCHEMBL5093.

Proteomic databases

PRIDEP0A9J6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76775; AAC76775; b3752.
BAE77536; BAE77536; BAE77536.
GeneID12930588.
948260.
KEGGecj:Y75_p3415.
eco:b3752.
PATRIC32123003. VBIEscCol129921_3877.

Organism-specific databases

EchoBASEEB0811.
EcoGeneEG10818. rbsK.

Phylogenomic databases

eggNOGCOG0524.
HOGENOMHOG000235950.
KOK00852.
OMATVIGKQY.
OrthoDBEOG64JFMD.
PhylomeDBP0A9J6.

Enzyme and pathway databases

BioCycEcoCyc:RIBOKIN-MONOMER.
ECOL316407:JW3731-MONOMER.
MetaCyc:RIBOKIN-MONOMER.
RETL1328306-WGS:GSTH-416-MONOMER.
RETL1328306-WGS:GSTH-5050-MONOMER.
UniPathwayUPA00916; UER00889.

Gene expression databases

GenevestigatorP0A9J6.

Family and domain databases

Gene3D3.40.1190.20. 1 hit.
InterProIPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011877. D_ribokin_bac.
IPR011611. PfkB_dom.
IPR002139. Ribokinase.
IPR029056. Ribokinase-like.
[Graphical view]
PfamPF00294. PfkB. 1 hit.
[Graphical view]
PRINTSPR00990. RIBOKINASE.
SUPFAMSSF53613. SSF53613. 1 hit.
TIGRFAMsTIGR02152. D_ribokin_bact. 1 hit.
PROSITEPS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0A9J6.
PROP0A9J6.

Entry information

Entry nameRBSK_ECOLI
AccessionPrimary (citable) accession number: P0A9J6
Secondary accession number(s): P05054, Q2M870
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: August 13, 1987
Last modified: July 9, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene