Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2-dehydropantoate 2-reductase

Gene

panE

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid.By similarity

Catalytic activityi

(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB), 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB), 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)
  2. 2-dehydropantoate 2-reductase (panE), 2-dehydropantoate 2-reductase (AJR26_001125), 2-dehydropantoate 2-reductase (AJR25_005345)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31NADP; via amide nitrogenBy similarity1
Binding sitei98NADP; via amide nitrogenBy similarity1
Binding sitei98SubstrateBy similarity1
Binding sitei122NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Active sitei176Proton donorBy similarity1
Binding sitei180SubstrateBy similarity1
Binding sitei184SubstrateBy similarity1
Binding sitei194SubstrateBy similarity1
Binding sitei241SubstrateBy similarity1
Binding sitei244SubstrateBy similarity1
Binding sitei256NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 12NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processPantothenate biosynthesis
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00028; UER00004.

Names & Taxonomyi

Protein namesi
Recommended name:
2-dehydropantoate 2-reductase (EC:1.1.1.169)
Alternative name(s):
Ketopantoate reductase
Short name:
KPA reductase
Short name:
KPR
Gene namesi
Name:panE
Synonyms:apbA
Ordered Locus Names:SF0362, S0370
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001573051 – 3032-dehydropantoate 2-reductaseAdd BLAST303

Proteomic databases

PaxDbiP0A9J5.
PRIDEiP0A9J5.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0A9J5.
SMRiP0A9J5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ketopantoate reductase family.Curated

Phylogenomic databases

eggNOGiENOG4108JZF. Bacteria.
COG1893. LUCA.
HOGENOMiHOG000050223.
KOiK00077.
OMAiLTVLHDC.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
InterProiView protein in InterPro
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR003710. ApbA.
IPR013752. KPA_reductase.
IPR013332. KPR_N.
IPR016040. NAD(P)-bd_dom.
PfamiView protein in Pfam
PF02558. ApbA. 1 hit.
PF08546. ApbA_C. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00745. apbA_panE. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A9J5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKITVLGCGA LGQLWLTALC KQGHEVQGWL RVPQPYCSVN LVETDGSIFN
60 70 80 90 100
ESLTANDPDF LATSDLLLVT LKAWQVSDAV KSLASTLPVT TPILLIHNGM
110 120 130 140 150
GTIEELQNIQ QPLLMGTTTH AARRDGNVII HVANGITHIG PARQQDGDYS
160 170 180 190 200
YLADILQTVL PDVAWHNNIR AELWRKLAVN CVINPLTAIW NCPNGELRHH
210 220 230 240 250
PQEIMQICEE VAAVIEREGH HTSAEDLRDY VMQVIDATAE NISSMLQDIR
260 270 280 290 300
ALRHTEIDYI NGFLLRRARA HGIAVPENTR LFEMVKRKES EYERIGTGLP

RPW
Length:303
Mass (Da):33,871
Last modified:July 19, 2005 - v1
Checksum:iED7027BFE6F86D6F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005674 Genomic DNA. Translation: AAN42020.1.
AE014073 Genomic DNA. Translation: AAP15896.1.
RefSeqiNP_706313.1. NC_004337.2.
WP_000705865.1. NZ_MSKF02000045.1.

Genome annotation databases

EnsemblBacteriaiAAN42020; AAN42020; SF0362.
AAP15896; AAP15896; S0370.
GeneIDi1027702.
KEGGisfl:SF0362.
sfx:S0370.
PATRICifig|198214.7.peg.415.

Similar proteinsi

Entry informationi

Entry nameiPANE_SHIFL
AccessioniPrimary (citable) accession number: P0A9J5
Secondary accession number(s): P77728, Q46947
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: September 27, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families