P0A9J5 (PANE_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-dehydropantoate 2-reductase
EC=1.1.1.169

Alternative name(s):
Ketopantoate reductase
Short name=KPA reductase
Short name=KPR

Gene names

Name:	panE
Synonyms:	apbA
Ordered Locus Names:	SF0362, S0370

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of ketopantoate into pantoic acid By similarity.
Catalytic activity	(R)-pantoate + NADP+ = 2-dehydropantoate + NADPH.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 2/2.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the ketopantoate reductase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2-dehydropantoate 2-reductase activity Inferred from electronic annotation. Source: EC NADP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	2-dehydropantoate 2-reductase		PRO_0000157305
Regions
	Nucleotide binding	7 – 12	6	NADP By similarity
Sites
	Active site	176	1	Proton donor By similarity
	Binding site	31	1	NADP; via amide nitrogen By similarity
	Binding site	98	1	NADP; via amide nitrogen By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	122	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	180	1	Substrate By similarity
	Binding site	184	1	Substrate By similarity
	Binding site	194	1	Substrate By similarity
	Binding site	241	1	Substrate By similarity
	Binding site	244	1	Substrate By similarity
	Binding site	256	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A9J5 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: ED7027BFE6F86D6F	FASTA	303	33,871
	10 20 30 40 50 60 MKITVLGCGA LGQLWLTALC KQGHEVQGWL RVPQPYCSVN LVETDGSIFN ESLTANDPDF 70 80 90 100 110 120 LATSDLLLVT LKAWQVSDAV KSLASTLPVT TPILLIHNGM GTIEELQNIQ QPLLMGTTTH 130 140 150 160 170 180 AARRDGNVII HVANGITHIG PARQQDGDYS YLADILQTVL PDVAWHNNIR AELWRKLAVN 190 200 210 220 230 240 CVINPLTAIW NCPNGELRHH PQEIMQICEE VAAVIEREGH HTSAEDLRDY VMQVIDATAE 250 260 270 280 290 300 NISSMLQDIR ALRHTEIDYI NGFLLRRARA HGIAVPENTR LFEMVKRKES EYERIGTGLP RPW

References

[1]

"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.

[2]

"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN42020.1. AE014073 Genomic DNA. Translation: AAP15896.1.
RefSeq	NP_706313.1. NC_004337.2. NP_836090.1. NC_004741.1.
3D structure databases
ProteinModelPortal	P0A9J5.
SMR	P0A9J5. Positions 1-292.
ModBase	Search...
Protein-protein interaction databases
STRING	198214.SF0362.
Proteomic databases
PaxDb	P0A9J5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN42020; AAN42020; SF0362. AAP15896; AAP15896; S0370.
GeneID	1027702. 1076803.
KEGG	sfl:SF0362. sfx:S0370.
PATRIC	18701740. VBIShiFle31049_0403.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1893.
HOGENOM	HOG000050223.
KO	K00077.
OMA	IVCVKAY.
ProtClustDB	PRK06522.
Enzyme and pathway databases
UniPathway	UPA00028; UER00004.
Family and domain databases
Gene3D	1.10.1040.10. 1 hit. 3.40.50.720. 1 hit.
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR003710. ApbA. IPR013752. ApbA_C. IPR013332. ApbA_N. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF02558. ApbA. 1 hit. PF08546. ApbA_C. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00745. apbA_panE. 1 hit.
ProtoNet	Search...
Other
BindingDB	P0A9J5.

Entry information

Entry name

PANE_SHIFL

Accession

Primary (citable) accession number: P0A9J5
Secondary accession number(s): P77728, Q46947

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program