Reviewed, UniProtKB/Swiss-Prot P0A9J0 (RNG_ECOLI)

Names and origin

Protein names

Recommended name:
    Ribonuclease G
      Short name=RNase G
    EC=3.1.26.-
Alternative name(s):
    Cytoplasmic axial filament protein

Gene names

Name:	rng
Synonyms:	cafA, yhdF
Ordered Locus Names:	b3247, JW3216

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	489 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure. Ref.5
Subcellular location	Cytoplasm › cytoskeleton.
Sequence similarities	Belongs to the RNase E/G family. RNase G subfamily. Contains 1 S1 motif domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cytoplasm Cytoskeleton
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
rpsD	P0A7V8	1	EBI-545964,EBI-543939

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 489	488	Ribonuclease G		PRO_0000097383
Regions
	Domain	39 – 128	90	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	P0A9J0-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 9517B3367C455C99	FASTA	489	55,364
	10 20 30 40 50 60 MTAELLVNVT PSETRVAYID GGILQEIHIE REARRGIVGN IYKGRVSRVL PGMQAAFVDI 70 80 90 100 110 120 GLDKAAFLHA SDIMPHTECV AGEEQKQFTV RDISELVRQG QDLMVQVVKD PLGTKGARLT 130 140 150 160 170 180 TDITLPSRYL VFMPGASHVG VSQRIESESE RERLKKVVAE YCDEQGGFII RTAAEGVGEA 190 200 210 220 230 240 ELASDAAYLK RVWTKVMERK KRPQTRYQLY GELALAQRVL RDFADAELDR IRVDSRLTYE 250 260 270 280 290 300 ALLEFTSEYI PEMTSKLEHY TGRQPIFDLF DVENEIQRAL ERKVELKSGG YLIIDQTEAM 310 320 330 340 350 360 TTVDINTGAF VGHRNLDDTI FNTNIEATQA IARQLRLRNL GGIIIIDFID MNNEDHRRRV 370 380 390 400 410 420 LHSLEQALSK DRVKTSVNGF SALGLVEMTR KRTRESIEHV LCNECPTCHG RGTVKTVETV 430 440 450 460 470 480 CYEIMREIVR VHHAYDSDRF LVYASPAVAE ALKGEESHSL AEVEIFVGKQ VKVQIEPLYN QEQFDVVMM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of the downstream flanking region of the shape-determining genes mreBCD of Escherichia coli." Wachi M., Doi M., Ueda T., Ueki M., Tsuritani K., Nagai K., Matsuhashi M. Gene 106:135-136(1991) [PubMed: 1937035] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Cytoplasmic axial filaments in Escherichia coli cells: possible function in the mechanism of chromosome segregation and cell division." Okada Y., Wachi M., Hirata A., Suzuki K., Nagai K., Matsuhashi M. J. Bacteriol. 176:917-922(1994) [PubMed: 8300545] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11, CHARACTERIZATION.
[5]	"Escherichia coli cafA gene encodes a novel RNase, designated as RNase G, involved in processing of the 5' end of 16S rRNA." Wachi M., Umitsuki G., Shimizu M., Takada A., Nagai K. Biochem. Biophys. Res. Commun. 259:483-488(1999) [PubMed: 10362534] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X57166 Genomic DNA. Translation: CAA40457.1. U18997 Genomic DNA. Translation: AAA58050.1. Different initiation. U00096 Genomic DNA. Translation: AAC76279.1. Different initiation. AP009048 Genomic DNA. Translation: BAE77289.1.
PIR	A65117.
RefSeq	AP_003788.1. NP_417713.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A9J0. 19 interactions.
Genome annotation databases
GeneID	947744.
GenomeReviews	Gene locus JW3216 in contig AP009048_GR. Gene locus b3247 in contig U00096_GR.
KEGG	ecj:JW3216. eco:b3247.
Organism-specific databases
EchoBASE	EB1276.
EcoGene	EG11299. rng.
CMR	Search...
Phylogenomic databases
HOGENOM	P0A9J0.
OMA	P0A9J0. HQRQVLR.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11299-MON.
Family and domain databases
InterPro	IPR012340. NA-bd_OB-fold. IPR003029. Rbsml_prot_S1_RNA-bd_dom. IPR019307. RNA-bd_AU-1/RNase_E/G. IPR004659. RNaseE/G. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF10150. RNase_E_G. 1 hit. PF00575. S1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00757. RNaseEG. 1 hit.
PROSITE	PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNG_ECOLI

Accession

Primary (citable) accession number: P0A9J0
Secondary accession number(s): P25537, P76677, Q2M8W7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 36 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)