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Protein

Ribonuclease G

Gene

rng

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi304Magnesium; catalyticBy similarity1
Metal bindingi347Magnesium; catalyticBy similarity1

GO - Molecular functioni

  • endonuclease activity Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • ribonuclease G activity Source: EcoliWiki
  • RNA binding Source: InterPro

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • rRNA processing Source: EcoliWiki

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processCell cycle, Cell division
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11299-MONOMER
MetaCyc:EG11299-MONOMER
BRENDAi3.1.26.3 2165

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease G (EC:3.1.26.-)
Short name:
RNase G
Alternative name(s):
Cytoplasmic axial filament protein
Gene namesi
Name:rng
Synonyms:cafA, yhdF
Ordered Locus Names:b3247, JW3216
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11299 rng

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000973832 – 489Ribonuclease GAdd BLAST488

Proteomic databases

EPDiP0A9J0
PaxDbiP0A9J0
PRIDEiP0A9J0

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
rpsDP0A7V82EBI-545964,EBI-543939

Protein-protein interaction databases

BioGridi4262071, 231 interactors
DIPiDIP-48098N
IntActiP0A9J0, 20 interactors
STRINGi316385.ECDH10B_3423

Structurei

3D structure databases

ProteinModelPortaliP0A9J0
SMRiP0A9J0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 128S1 motifPROSITE-ProRule annotationAdd BLAST90

Sequence similaritiesi

Belongs to the RNase E/G family. RNase G subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105C03 Bacteria
COG1530 LUCA
HOGENOMiHOG000258025
InParanoidiP0A9J0
KOiK08301
OMAiAFLHYHD
PhylomeDBiP0A9J0

Family and domain databases

InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR019307 RNA-bd_AU-1/RNase_E/G
IPR004659 RNase_E/G
IPR022967 S1_dom
IPR003029 S1_domain
PfamiView protein in Pfam
PF10150 RNase_E_G, 1 hit
PF00575 S1, 1 hit
SMARTiView protein in SMART
SM00316 S1, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00757 RNaseEG, 1 hit
PROSITEiView protein in PROSITE
PS50126 S1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAELLVNVT PSETRVAYID GGILQEIHIE REARRGIVGN IYKGRVSRVL
60 70 80 90 100
PGMQAAFVDI GLDKAAFLHA SDIMPHTECV AGEEQKQFTV RDISELVRQG
110 120 130 140 150
QDLMVQVVKD PLGTKGARLT TDITLPSRYL VFMPGASHVG VSQRIESESE
160 170 180 190 200
RERLKKVVAE YCDEQGGFII RTAAEGVGEA ELASDAAYLK RVWTKVMERK
210 220 230 240 250
KRPQTRYQLY GELALAQRVL RDFADAELDR IRVDSRLTYE ALLEFTSEYI
260 270 280 290 300
PEMTSKLEHY TGRQPIFDLF DVENEIQRAL ERKVELKSGG YLIIDQTEAM
310 320 330 340 350
TTVDINTGAF VGHRNLDDTI FNTNIEATQA IARQLRLRNL GGIIIIDFID
360 370 380 390 400
MNNEDHRRRV LHSLEQALSK DRVKTSVNGF SALGLVEMTR KRTRESIEHV
410 420 430 440 450
LCNECPTCHG RGTVKTVETV CYEIMREIVR VHHAYDSDRF LVYASPAVAE
460 470 480
ALKGEESHSL AEVEIFVGKQ VKVQIEPLYN QEQFDVVMM
Length:489
Mass (Da):55,364
Last modified:January 23, 2007 - v2
Checksum:i9517B3367C455C99
GO

Sequence cautioni

The sequence AAA58050 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57166 Genomic DNA Translation: CAA40457.1
U18997 Genomic DNA Translation: AAA58050.1 Different initiation.
U00096 Genomic DNA Translation: AAC76279.2
AP009048 Genomic DNA Translation: BAE77289.1
PIRiA65117
RefSeqiNP_417713.2, NC_000913.3
WP_000123197.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76279; AAC76279; b3247
BAE77289; BAE77289; BAE77289
GeneIDi947744
KEGGiecj:JW3216
eco:b3247
PATRICifig|1411691.4.peg.3482

Similar proteinsi

Entry informationi

Entry nameiRNG_ECOLI
AccessioniPrimary (citable) accession number: P0A9J0
Secondary accession number(s): P25537, P76677, Q2M8W7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 105 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health