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Protein

Ribonuclease G

Gene

rng

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure.1 Publication

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi304Magnesium; catalyticBy similarity1
Metal bindingi347Magnesium; catalyticBy similarity1

GO - Molecular functioni

  • endonuclease activity Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • ribonuclease G activity Source: EcoliWiki
  • RNA binding Source: InterPro

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • rRNA processing Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11299-MONOMER.
ECOL316407:JW3216-MONOMER.
MetaCyc:EG11299-MONOMER.
BRENDAi3.1.26.3. 2165.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease G (EC:3.1.26.-)
Short name:
RNase G
Alternative name(s):
Cytoplasmic axial filament protein
Gene namesi
Name:rng
Synonyms:cafA, yhdF
Ordered Locus Names:b3247, JW3216
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11299. rng.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000973832 – 489Ribonuclease GAdd BLAST488

Proteomic databases

EPDiP0A9J0.
PaxDbiP0A9J0.
PRIDEiP0A9J0.

Interactioni

Protein-protein interaction databases

BioGridi4262071. 182 interactors.
DIPiDIP-48098N.
IntActiP0A9J0. 20 interactors.
MINTiMINT-1221298.
STRINGi511145.b3247.

Structurei

3D structure databases

ProteinModelPortaliP0A9J0.
SMRiP0A9J0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 128S1 motifPROSITE-ProRule annotationAdd BLAST90

Sequence similaritiesi

Belongs to the RNase E/G family. RNase G subfamily.Curated
Contains 1 S1 motif domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C03. Bacteria.
COG1530. LUCA.
HOGENOMiHOG000258025.
InParanoidiP0A9J0.
KOiK08301.
OMAiHQRQVLR.
PhylomeDBiP0A9J0.

Family and domain databases

InterProiIPR012340. NA-bd_OB-fold.
IPR019307. RNA-bd_AU-1/RNase_E/G.
IPR004659. RNase_E/G.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF10150. RNase_E_G. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00757. RNaseEG. 1 hit.
PROSITEiPS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAELLVNVT PSETRVAYID GGILQEIHIE REARRGIVGN IYKGRVSRVL
60 70 80 90 100
PGMQAAFVDI GLDKAAFLHA SDIMPHTECV AGEEQKQFTV RDISELVRQG
110 120 130 140 150
QDLMVQVVKD PLGTKGARLT TDITLPSRYL VFMPGASHVG VSQRIESESE
160 170 180 190 200
RERLKKVVAE YCDEQGGFII RTAAEGVGEA ELASDAAYLK RVWTKVMERK
210 220 230 240 250
KRPQTRYQLY GELALAQRVL RDFADAELDR IRVDSRLTYE ALLEFTSEYI
260 270 280 290 300
PEMTSKLEHY TGRQPIFDLF DVENEIQRAL ERKVELKSGG YLIIDQTEAM
310 320 330 340 350
TTVDINTGAF VGHRNLDDTI FNTNIEATQA IARQLRLRNL GGIIIIDFID
360 370 380 390 400
MNNEDHRRRV LHSLEQALSK DRVKTSVNGF SALGLVEMTR KRTRESIEHV
410 420 430 440 450
LCNECPTCHG RGTVKTVETV CYEIMREIVR VHHAYDSDRF LVYASPAVAE
460 470 480
ALKGEESHSL AEVEIFVGKQ VKVQIEPLYN QEQFDVVMM
Length:489
Mass (Da):55,364
Last modified:January 23, 2007 - v2
Checksum:i9517B3367C455C99
GO

Sequence cautioni

The sequence AAA58050 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57166 Genomic DNA. Translation: CAA40457.1.
U18997 Genomic DNA. Translation: AAA58050.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76279.2.
AP009048 Genomic DNA. Translation: BAE77289.1.
PIRiA65117.
RefSeqiNP_417713.2. NC_000913.3.
WP_000123197.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76279; AAC76279; b3247.
BAE77289; BAE77289; BAE77289.
GeneIDi947744.
KEGGiecj:JW3216.
eco:b3247.
PATRICi32121920. VBIEscCol129921_3344.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57166 Genomic DNA. Translation: CAA40457.1.
U18997 Genomic DNA. Translation: AAA58050.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76279.2.
AP009048 Genomic DNA. Translation: BAE77289.1.
PIRiA65117.
RefSeqiNP_417713.2. NC_000913.3.
WP_000123197.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A9J0.
SMRiP0A9J0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262071. 182 interactors.
DIPiDIP-48098N.
IntActiP0A9J0. 20 interactors.
MINTiMINT-1221298.
STRINGi511145.b3247.

Proteomic databases

EPDiP0A9J0.
PaxDbiP0A9J0.
PRIDEiP0A9J0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76279; AAC76279; b3247.
BAE77289; BAE77289; BAE77289.
GeneIDi947744.
KEGGiecj:JW3216.
eco:b3247.
PATRICi32121920. VBIEscCol129921_3344.

Organism-specific databases

EchoBASEiEB1276.
EcoGeneiEG11299. rng.

Phylogenomic databases

eggNOGiENOG4105C03. Bacteria.
COG1530. LUCA.
HOGENOMiHOG000258025.
InParanoidiP0A9J0.
KOiK08301.
OMAiHQRQVLR.
PhylomeDBiP0A9J0.

Enzyme and pathway databases

BioCyciEcoCyc:EG11299-MONOMER.
ECOL316407:JW3216-MONOMER.
MetaCyc:EG11299-MONOMER.
BRENDAi3.1.26.3. 2165.

Miscellaneous databases

PROiP0A9J0.

Family and domain databases

InterProiIPR012340. NA-bd_OB-fold.
IPR019307. RNA-bd_AU-1/RNase_E/G.
IPR004659. RNase_E/G.
IPR022967. S1_dom.
IPR003029. S1_domain.
[Graphical view]
PfamiPF10150. RNase_E_G. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00757. RNaseEG. 1 hit.
PROSITEiPS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNG_ECOLI
AccessioniPrimary (citable) accession number: P0A9J0
Secondary accession number(s): P25537, P76677, Q2M8W7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.