ID   NIRD_ECO57              Reviewed;         108 AA.
AC   P0A9I9; P23675;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   16-JUN-2009, entry version 30.
DE   RecName: Full=Nitrite reductase [NAD(P)H] small subunit;
DE            EC=1.7.1.4;
GN   Name=nirD; OrderedLocusNames=Z4727, ECs4217;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   MEDLINE=21074935; PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA   Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA   Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA   Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA   Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA   Welch R.A., Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   MEDLINE=21156231; PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA   Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA   Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli
RT   O157:H7 and genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Required for activity of the reductase (By similarity).
CC   -!- CATALYTIC ACTIVITY: Ammonium hydroxide + 3 NAD(P)(+) + H(2)O =
CC       nitrite + 3 NAD(P)H.
CC   -!- SUBUNIT: Associates with nirB (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: To B.subtilis nasE.
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DR   EMBL; AE005174; AAG58474.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB37640.1; -; Genomic_DNA.
DR   PIR; A91156; A91156.
DR   PIR; F86001; F86001.
DR   RefSeq; NP_289914.1; -.
DR   RefSeq; NP_312244.1; -.
DR   SMR; P0A9I9; 1-108.
DR   GeneID; 915916; -.
DR   GeneID; 958830; -.
DR   GenomeReviews; AE005174_GR; Z4727.
DR   GenomeReviews; BA000007_GR; ECs4217.
DR   KEGG; ece:Z4727; -.
DR   KEGG; ecs:ECs4217; -.
DR   HOGENOM; P0A9I9; -.
DR   OMA; P0A9I9; GVCALVE.
DR   BioCyc; ECOL83334:ECS4217-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0008942; F:nitrite reductase [NAD(P)H] activity; IEA:EC.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR012748; Nitri_red_NirD.
DR   InterPro; IPR017881; Nitrite_Rdtase_lsu_NirD_bac.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   Gene3D; G3DSA:2.102.10.10; Rieske_reg; 1.
DR   TIGRFAMs; TIGR02378; nirD_assim_sml; 1.
DR   PROSITE; PS51300; NIRD; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; NAD; Nitrate assimilation;
KW   Oxidoreductase.
FT   CHAIN         1    108       Nitrite reductase [NAD(P)H] small
FT                                subunit.
FT                                /FTId=PRO_0000096854.
SQ   SEQUENCE   108 AA;  12284 MW;  4B16AAE73EC4B786 CRC64;
     MSQWKDICKI DDILPETGVC ALLGDEQVAI FRPYHSDQVF AISNIDPFFE SSVLSRGLIA
     EHQGELWVAS PLKKQRFRLS DGLCMEDEQF SVKHYEARVK DGVVQLRG
//