Reviewed, UniProtKB/Swiss-Prot P0A9I9 (NIRD_ECO57)

Names and origin

Protein names

Recommended name:
    Nitrite reductase [NAD(P)H] small subunit
    EC=1.7.1.4

Gene names

Name:	nirD
Ordered Locus Names:	Z4727, ECs4217

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	108 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required for activity of the reductase By similarity.
Catalytic activity	Ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H.
Subunit structure	Associates with nirB By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	To B.subtilis nasE.

Ontologies

Keywords
Biological process	Nitrate assimilation
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: InterPro nitrite reductase [NAD(P)H] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 108	108	Nitrite reductase [NAD(P)H] small subunit		PRO_0000096854

Sequences

	Sequence		Length	Mass (Da)
	P0A9I9-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 4B16AAE73EC4B786	FASTA	108	12,284
	10 20 30 40 50 60 MSQWKDICKI DDILPETGVC ALLGDEQVAI FRPYHSDQVF AISNIDPFFE SSVLSRGLIA 70 80 90 100 EHQGELWVAS PLKKQRFRLS DGLCMEDEQF SVKHYEARVK DGVVQLRG

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG58474.1. BA000007 Genomic DNA. Translation: BAB37640.1.
PIR	A91156. F86001.
RefSeq	NP_289914.1. NP_312244.1.
3D structure databases
SMR	P0A9I9. Positions 1-108.
ModBase	Search...
Genome annotation databases
GeneID	915916. 958830.
GenomeReviews	Gene locus Z4727 in contig AE005174_GR. Gene locus ECs4217 in contig BA000007_GR.
KEGG	ece:Z4727. ecs:ECs4217.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG668715.
OMA	GVCALVE.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS4217-MONOMER.
Family and domain databases
InterPro	IPR012748. Nitri_red_NirD. IPR017881. Nitrite_Rdtase_lsu_NirD_bac. IPR017941. Rieske_2Fe-2S. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
TIGRFAMs	TIGR02378. nirD_assim_sml. 1 hit.
PROSITE	PS51300. NIRD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIRD_ECO57

Accession

Primary (citable) accession number: P0A9I9
Secondary accession number(s): P23675

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)