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P0A9I9 (NIRD_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nitrite reductase [NAD(P)H] small subunit
EC=1.7.1.4
Gene names
Name:nirD
Ordered Locus Names:Z4727, ECs4217
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length108 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for activity of the reductase By similarity.

Catalytic activity

Ammonium hydroxide + 3 NAD(P)+ + H2O = nitrite + 3 NAD(P)H.

Subunit structure

Associates with nirB By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

To B.subtilis nasE.

Ontologies

Keywords
   Biological processNitrate assimilation
   Cellular componentCytoplasm
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processnitrate assimilation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function2 iron, 2 sulfur cluster binding

Inferred from electronic annotation. Source: InterPro

nitrite reductase [NAD(P)H] activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 108108Nitrite reductase [NAD(P)H] small subunit
PRO_0000096854

Sequences

Sequence LengthMass (Da)Tools
P0A9I9 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: 4B16AAE73EC4B786

FASTA10812,284
        10         20         30         40         50         60 
MSQWKDICKI DDILPETGVC ALLGDEQVAI FRPYHSDQVF AISNIDPFFE SSVLSRGLIA 

        70         80         90        100 
EHQGELWVAS PLKKQRFRLS DGLCMEDEQF SVKHYEARVK DGVVQLRG

« Hide

References

[1]"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7."
Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. expand/collapse author list , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R.
Nature 409:529-533(2001) [PubMed: 11206551] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
[2]"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. expand/collapse author list , Kuhara S., Shiba T., Hattori M., Shinagawa H.
DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE005174 Genomic DNA. Translation: AAG58474.1.
BA000007 Genomic DNA. Translation: BAB37640.1.
PIRA91156.
F86001.
RefSeqNP_289914.1. NC_002655.2.
NP_312244.1. NC_002695.1.

3D structure databases

ProteinModelPortalP0A9I9.
SMRP0A9I9. Positions 1-108.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000024716; EBESCP00000023609; EBESCG00000023770.
EBESCT00000060215; EBESCP00000058043; EBESCG00000059262.
GeneID915916.
958830.
GenomeReviewsGene locus Z4727 in contig AE005174_GR.
Gene locus ECs4217 in contig BA000007_GR.
KEGGece:Z4727.
ecs:ECs4217.
PATRIC18357984. VBIEscCol44059_4159.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000011425.
HOGENOMHBG668715.
OMAEDERFNV.
ProtClustDBPRK09511.

Enzyme and pathway databases

BioCycECOL83334:ECS4217-MONOMER.

Family and domain databases

InterProIPR012748. Nitri_red_NirD.
IPR017881. Nitrite_Rdtase_lsu_NirD_bac.
IPR017941. Rieske_2Fe-2S.
[Graphical view]
Gene3DG3DSA:2.102.10.10. Rieske_reg. 1 hit.
KOK00363.
SUPFAMSSF50022. Rieske_dom. 1 hit.
TIGRFAMsTIGR02378. NirD_assim_sml. 1 hit.
PROSITEPS51300. NIRD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNIRD_ECO57
AccessionPrimary (citable) accession number: P0A9I9
Secondary accession number(s): P23675
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program
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