ID   NIRD_ECOLI              Reviewed;         108 AA.
AC   P0A9I8; P23675; Q2M732;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   16-JUN-2009, entry version 37.
DE   RecName: Full=Nitrite reductase [NAD(P)H] small subunit;
DE            EC=1.7.1.4;
GN   Name=nirD; OrderedLocusNames=b3366, JW3329;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   MEDLINE=89282391; PubMed=2543955; DOI=10.1093/nar/17.10.3865;
RA   Bell A.I., Gaston K.L., Cole J.A., Busby S.J.W.;
RT   "Cloning of binding sequences for the Escherichia coli transcription
RT   activators, FNR and CRP: location of bases involved in discrimination
RT   between FNR and CRP.";
RL   Nucleic Acids Res. 17:3865-3874(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   MEDLINE=90345936; PubMed=2200672;
RX   DOI=10.1111/j.1432-1033.1990.tb19125.x;
RA   Peakman T., Crouzet J., Mayaux J.F., Busby S.J.W., Mohan S.,
RA   Harborne N., Wootton J., Nicolson R., Cole J.A.;
RT   "Nucleotide sequence, organisation and structural analysis of the
RT   products of genes in the nirB-cysG region of the Escherichia coli K-12
RT   chromosome.";
RL   Eur. J. Biochem. 191:315-323(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   FUNCTION.
RX   MEDLINE=93062014; PubMed=1435259;
RX   DOI=10.1111/j.1365-2958.1992.tb01460.x;
RA   Harborne N., Griffiths L., Busby S.J., Cole J.A.;
RT   "Transcriptional control, translation and function of the products of
RT   the five open reading frames of the Escherichia coli nir operon.";
RL   Mol. Microbiol. 6:2805-2813(1992).
CC   -!- FUNCTION: Required for activity of the reductase.
CC   -!- CATALYTIC ACTIVITY: Ammonium hydroxide + 3 NAD(P)(+) + H(2)O =
CC       nitrite + 3 NAD(P)H.
CC   -!- SUBUNIT: Associates with nirB.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: To B.subtilis nasE.
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DR   EMBL; X14202; CAA32417.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; U18997; AAA58163.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76391.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77924.1; -; Genomic_DNA.
DR   PIR; A65131; A65131.
DR   RefSeq; AP_004423.1; -.
DR   RefSeq; NP_417825.1; -.
DR   PDB; 2JO6; NMR; -; A=1-108.
DR   PDBsum; 2JO6; -.
DR   GeneID; 947881; -.
DR   GenomeReviews; AP009048_GR; JW3329.
DR   GenomeReviews; U00096_GR; b3366.
DR   KEGG; ecj:JW3329; -.
DR   KEGG; eco:b3366; -.
DR   EchoBASE; EB0649; -.
DR   EcoGene; EG10655; nirD.
DR   HOGENOM; P0A9I8; -.
DR   OMA; P0A9I8; GVCALVE.
DR   BioCyc; EcoCyc:NIRD-MON; -.
DR   BioCyc; MetaCyc:NIRD-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0008942; F:nitrite reductase [NAD(P)H] activity; IEA:EC.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR012748; Nitri_red_NirD.
DR   InterPro; IPR017881; Nitrite_Rdtase_lsu_NirD_bac.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   Gene3D; G3DSA:2.102.10.10; Rieske_reg; 1.
DR   TIGRFAMs; TIGR02378; nirD_assim_sml; 1.
DR   PROSITE; PS51300; NIRD; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Complete proteome; Cytoplasm; NAD; Nitrate assimilation;
KW   Oxidoreductase.
FT   CHAIN         1    108       Nitrite reductase [NAD(P)H] small
FT                                subunit.
FT                                /FTId=PRO_0000096855.
FT   CONFLICT    102    102       G -> D (in Ref. 1 and 2).
FT   STRAND        5      9
FT   TURN         10     12
FT   STRAND       17     23
FT   STRAND       26     32
FT   STRAND       34     37
FT   STRAND       40     45
FT   STRAND       47     49
FT   STRAND       57     62
FT   STRAND       65     70
FT   TURN         71     74
FT   STRAND       75     78
FT   TURN         79     82
FT   TURN         85     90
FT   STRAND       94    100
FT   STRAND      103    107
SQ   SEQUENCE   108 AA;  12284 MW;  4B16AAE73EC4B786 CRC64;
     MSQWKDICKI DDILPETGVC ALLGDEQVAI FRPYHSDQVF AISNIDPFFE SSVLSRGLIA
     EHQGELWVAS PLKKQRFRLS DGLCMEDEQF SVKHYEARVK DGVVQLRG
//