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Protein

Nitrite reductase (NADH) small subunit

Gene

nirD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for activity of the reductase.1 Publication

Catalytic activityi

Ammonia + 3 NAD+ + 2 H2O = nitrite + 3 NADH.

GO - Molecular functioni

GO - Biological processi

  • anaerobic respiration Source: EcoCyc
  • nitrate assimilation Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processNitrate assimilation
LigandNAD

Enzyme and pathway databases

BioCyciEcoCyc:NIRD-MONOMER
MetaCyc:NIRD-MONOMER
BRENDAi1.7.1.15 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrite reductase (NADH) small subunit (EC:1.7.1.15)
Gene namesi
Name:nirD
Ordered Locus Names:b3366, JW3329
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10655 nirD

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nitrite reductase complex [NAD(P)H] Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000968551 – 108Nitrite reductase (NADH) small subunitAdd BLAST108

Proteomic databases

PaxDbiP0A9I8
PRIDEiP0A9I8

Interactioni

Subunit structurei

Associates with NirB.

Protein-protein interaction databases

BioGridi4262476, 15 interactors
STRINGi316385.ECDH10B_3542

Structurei

Secondary structure

1108
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Turni10 – 12Combined sources3
Beta strandi17 – 23Combined sources7
Beta strandi26 – 32Combined sources7
Beta strandi34 – 37Combined sources4
Beta strandi40 – 45Combined sources6
Beta strandi47 – 49Combined sources3
Beta strandi57 – 62Combined sources6
Beta strandi65 – 70Combined sources6
Turni71 – 74Combined sources4
Beta strandi75 – 78Combined sources4
Turni79 – 82Combined sources4
Turni85 – 90Combined sources6
Beta strandi94 – 100Combined sources7
Beta strandi103 – 107Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JO6NMR-A1-108[»]
ProteinModelPortaliP0A9I8
SMRiP0A9I8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9I8

Family & Domainsi

Sequence similaritiesi

To B.subtilis NasE.Curated

Phylogenomic databases

eggNOGiENOG4105WZK Bacteria
COG2146 LUCA
HOGENOMiHOG000128543
InParanoidiP0A9I8
KOiK00363
OMAiLRDGLCM
PhylomeDBiP0A9I8

Family and domain databases

CDDicd03529 Rieske_NirD, 1 hit
Gene3Di2.102.10.10, 1 hit
InterProiView protein in InterPro
IPR012748 Nitri_red_NirD
IPR017881 Nitrite_Rdtase_lsu_NirD_bac
IPR036922 Rieske_2Fe-2S_sf
PANTHERiPTHR40562 PTHR40562, 1 hit
PfamiView protein in Pfam
PF13806 Rieske_2, 1 hit
SUPFAMiSSF50022 SSF50022, 1 hit
TIGRFAMsiTIGR02378 nirD_assim_sml, 1 hit
PROSITEiView protein in PROSITE
PS51300 NIRD, 1 hit

Sequencei

Sequence statusi: Complete.

P0A9I8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQWKDICKI DDILPETGVC ALLGDEQVAI FRPYHSDQVF AISNIDPFFE
60 70 80 90 100
SSVLSRGLIA EHQGELWVAS PLKKQRFRLS DGLCMEDEQF SVKHYEARVK

DGVVQLRG
Length:108
Mass (Da):12,284
Last modified:July 19, 2005 - v1
Checksum:i4B16AAE73EC4B786
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102G → D (PubMed:2543955).Curated1
Sequence conflicti102G → D (PubMed:2200672).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14202 Genomic DNA Translation: CAA32417.1 Sequence problems.
U18997 Genomic DNA Translation: AAA58163.1
U00096 Genomic DNA Translation: AAC76391.1
AP009048 Genomic DNA Translation: BAE77924.1
PIRiA65131
RefSeqiNP_417825.1, NC_000913.3
WP_000084764.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76391; AAC76391; b3366
BAE77924; BAE77924; BAE77924
GeneIDi947881
KEGGiecj:JW3329
eco:b3366
PATRICifig|1411691.4.peg.3363

Similar proteinsi

Entry informationi

Entry nameiNIRD_ECOLI
AccessioniPrimary (citable) accession number: P0A9I8
Secondary accession number(s): P23675, Q2M732
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: March 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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