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Protein

Nitrite reductase (NADH) small subunit

Gene

nirD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for activity of the reductase.1 Publication

Catalytic activityi

Ammonia + 3 NAD+ + 2 H2O = nitrite + 3 NADH.

GO - Molecular functioni

GO - Biological processi

  • anaerobic respiration Source: EcoCyc
  • nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Nitrate assimilation

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciEcoCyc:NIRD-MONOMER.
ECOL316407:JW3329-MONOMER.
MetaCyc:NIRD-MONOMER.
BRENDAi1.7.1.15. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrite reductase (NADH) small subunit (EC:1.7.1.15)
Gene namesi
Name:nirD
Ordered Locus Names:b3366, JW3329
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10655. nirD.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nitrite reductase complex [NAD(P)H] Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000968551 – 108Nitrite reductase (NADH) small subunitAdd BLAST108

Proteomic databases

PaxDbiP0A9I8.
PRIDEiP0A9I8.

Interactioni

Subunit structurei

Associates with NirB.

Protein-protein interaction databases

BioGridi4262476. 13 interactors.
STRINGi511145.b3366.

Structurei

Secondary structure

1108
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Turni10 – 12Combined sources3
Beta strandi17 – 23Combined sources7
Beta strandi26 – 32Combined sources7
Beta strandi34 – 37Combined sources4
Beta strandi40 – 45Combined sources6
Beta strandi47 – 49Combined sources3
Beta strandi57 – 62Combined sources6
Beta strandi65 – 70Combined sources6
Turni71 – 74Combined sources4
Beta strandi75 – 78Combined sources4
Turni79 – 82Combined sources4
Turni85 – 90Combined sources6
Beta strandi94 – 100Combined sources7
Beta strandi103 – 107Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JO6NMR-A1-108[»]
ProteinModelPortaliP0A9I8.
SMRiP0A9I8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9I8.

Family & Domainsi

Sequence similaritiesi

To B.subtilis NasE.Curated

Phylogenomic databases

eggNOGiENOG4105WZK. Bacteria.
COG2146. LUCA.
HOGENOMiHOG000128543.
InParanoidiP0A9I8.
KOiK00363.
OMAiLRDGLCM.
PhylomeDBiP0A9I8.

Family and domain databases

CDDicd03529. Rieske_NirD. 1 hit.
Gene3Di2.102.10.10. 1 hit.
InterProiIPR012748. Nitri_red_NirD.
IPR017881. Nitrite_Rdtase_lsu_NirD_bac.
IPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF13806. Rieske_2. 1 hit.
[Graphical view]
SUPFAMiSSF50022. SSF50022. 1 hit.
TIGRFAMsiTIGR02378. nirD_assim_sml. 1 hit.
PROSITEiPS51300. NIRD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9I8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQWKDICKI DDILPETGVC ALLGDEQVAI FRPYHSDQVF AISNIDPFFE
60 70 80 90 100
SSVLSRGLIA EHQGELWVAS PLKKQRFRLS DGLCMEDEQF SVKHYEARVK

DGVVQLRG
Length:108
Mass (Da):12,284
Last modified:July 19, 2005 - v1
Checksum:i4B16AAE73EC4B786
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102G → D (PubMed:2543955).Curated1
Sequence conflicti102G → D (PubMed:2200672).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14202 Genomic DNA. Translation: CAA32417.1. Sequence problems.
U18997 Genomic DNA. Translation: AAA58163.1.
U00096 Genomic DNA. Translation: AAC76391.1.
AP009048 Genomic DNA. Translation: BAE77924.1.
PIRiA65131.
RefSeqiNP_417825.1. NC_000913.3.
WP_000084764.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76391; AAC76391; b3366.
BAE77924; BAE77924; BAE77924.
GeneIDi947881.
KEGGiecj:JW3329.
eco:b3366.
PATRICi32122166. VBIEscCol129921_3460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14202 Genomic DNA. Translation: CAA32417.1. Sequence problems.
U18997 Genomic DNA. Translation: AAA58163.1.
U00096 Genomic DNA. Translation: AAC76391.1.
AP009048 Genomic DNA. Translation: BAE77924.1.
PIRiA65131.
RefSeqiNP_417825.1. NC_000913.3.
WP_000084764.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JO6NMR-A1-108[»]
ProteinModelPortaliP0A9I8.
SMRiP0A9I8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262476. 13 interactors.
STRINGi511145.b3366.

Proteomic databases

PaxDbiP0A9I8.
PRIDEiP0A9I8.

Protocols and materials databases

DNASUi947881.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76391; AAC76391; b3366.
BAE77924; BAE77924; BAE77924.
GeneIDi947881.
KEGGiecj:JW3329.
eco:b3366.
PATRICi32122166. VBIEscCol129921_3460.

Organism-specific databases

EchoBASEiEB0649.
EcoGeneiEG10655. nirD.

Phylogenomic databases

eggNOGiENOG4105WZK. Bacteria.
COG2146. LUCA.
HOGENOMiHOG000128543.
InParanoidiP0A9I8.
KOiK00363.
OMAiLRDGLCM.
PhylomeDBiP0A9I8.

Enzyme and pathway databases

BioCyciEcoCyc:NIRD-MONOMER.
ECOL316407:JW3329-MONOMER.
MetaCyc:NIRD-MONOMER.
BRENDAi1.7.1.15. 2026.

Miscellaneous databases

EvolutionaryTraceiP0A9I8.
PROiP0A9I8.

Family and domain databases

CDDicd03529. Rieske_NirD. 1 hit.
Gene3Di2.102.10.10. 1 hit.
InterProiIPR012748. Nitri_red_NirD.
IPR017881. Nitrite_Rdtase_lsu_NirD_bac.
IPR017941. Rieske_2Fe-2S.
[Graphical view]
PfamiPF13806. Rieske_2. 1 hit.
[Graphical view]
SUPFAMiSSF50022. SSF50022. 1 hit.
TIGRFAMsiTIGR02378. nirD_assim_sml. 1 hit.
PROSITEiPS51300. NIRD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIRD_ECOLI
AccessioniPrimary (citable) accession number: P0A9I8
Secondary accession number(s): P23675, Q2M732
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.