P0A9I5 (NAPD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein napD

Gene names

Name:	napD
Synonyms:	yojF
Ordered Locus Names:	b2207, JW2195

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	87 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the correct assembly of subunits of the periplasmic napAB enzyme.
Subcellular location	Cytoplasm Potential.

Ontologies

Keywords
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of protein transport Inferred from direct assay. Source: EcoCyc
Cellular component	cytoplasm Inferred from direct assay. Source: EcoCyc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro signal sequence binding Inferred from physical interaction. Source: EcoCyc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
pta	P0A9M8	2	EBI-554985,EBI-555015
yajQ	P0A8E7	2	EBI-554985,EBI-370752

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 87

87

Protein napD

PRO_0000096713

Secondary structure

1

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87

Helix Strand Turn

Details...

Beta strand

5 – 14

10

Helix

16 – 28

13

Beta strand

29 – 31

3

Beta strand

33 – 36

4

Beta strand

40 – 49

10

Helix

53 – 65

13

Beta strand

69 – 73

5

Sequences

	Sequence		Length	Mass (Da)
	P0A9I5 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: EE59DF4EF17F650D	FASTA	87	9,469
	10 20 30 40 50 60 MHTNWQVCSL VVQAKSERIS DISTQLNAFP GCEVAVSDAP SGQLIVVVEA EDSETLIQTI 70 80 ESVRNVEGVL AVSLVYHQQE EQGEETP

References

[1]	"Automated multiplex sequencing of the E.coli genome." Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / BHB2600.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00008 Genomic DNA. Translation: AAA16400.1.
U00096 Genomic DNA. Translation: AAC75267.1.
AP009048 Genomic DNA. Translation: BAA15990.1.

PIR

E64990.

RefSeq

NP_416711.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JSX	NMR	-	A	1-87	[»]
2PQ4	NMR	-	A	1-87	[»]

ProteinModelPortal

P0A9I5.

SMR

P0A9I5. Positions 1-87.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-46322N.

IntAct

P0A9I5. 19 interactions.

MINT

MINT-1226955.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000001722; EBESCP00000001722; EBESCG00000001419.
EBESCT00000001723; EBESCP00000001723; EBESCG00000001419.
EBESCT00000001724; EBESCP00000001724; EBESCG00000001419.
EBESCT00000016973; EBESCP00000016264; EBESCG00000016032.

GeneID

945187.

GenomeReviews

Gene locus JW2195 in contig AP009048_GR.
Gene locus b2207 in contig U00096_GR.

KEGG

ecj:JW2195.
eco:b2207.

PATRIC

32119773. VBIEscCol129921_2296.

Organism-specific databases

EchoBASE

EB2064.

EcoGene

EG12143. napD.

Phylogenomic databases

eggNOG

COG3062.

GeneTree

EBGT00050000010847.

HOGENOM

HBG466530.

OMA

AKSERTS.

PhylomeDB

P0A9I5.

ProtClustDB

PRK10553.

Enzyme and pathway databases

BioCyc

EcoCyc:NAPD-MONOMER.

Gene expression databases

Genevestigator

P0A9I5.

Family and domain databases

InterPro

IPR005623. Chaperone_NapD_NO3_reduct.
IPR019887. Tscrpt_reg_AsnC-typ_C.
[Graphical view]

Gene3D

G3DSA:3.30.70.920. G3DSA:3.30.70.920. 1 hit.

KO

K02570.

Pfam

PF03927. NapD. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

NAPD_ECOLI

Accession

Primary (citable) accession number: P0A9I5
Secondary accession number(s): P33938

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program