Reviewed, UniProtKB/Swiss-Prot P0A9I5 (NAPD_ECOLI)

Names and origin

Protein names

Recommended name:
    Protein napD

Gene names

Name:	napD
Synonyms:	yojF
Ordered Locus Names:	b2207, JW2195

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	87 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a role in the correct assembly of subunits of the periplasmic napAB enzyme.
Subcellular location	Cytoplasm Potential.

Ontologies

Keywords
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
pta	P0A9M8	1	EBI-554985,EBI-555015
yajQ	P0A8E7	1	EBI-554985,EBI-370752

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 87

87

Protein napD

PRO_0000096713

Secondary structure

1

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87

Helix Strand Turn

Details...

Beta strand

5 – 14

10

Helix

16 – 28

13

Beta strand

29 – 31

3

Beta strand

33 – 36

4

Beta strand

40 – 49

10

Helix

53 – 65

13

Beta strand

69 – 73

5

Sequences

	Sequence		Length	Mass (Da)
	P0A9I5-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: EE59DF4EF17F650D	FASTA	87	9,469
	10 20 30 40 50 60 MHTNWQVCSL VVQAKSERIS DISTQLNAFP GCEVAVSDAP SGQLIVVVEA EDSETLIQTI 70 80 ESVRNVEGVL AVSLVYHQQE EQGEETP

References

[1]	"Automated multiplex sequencing of the E.coli genome." Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M. Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / BHB2600.
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U00008 Genomic DNA. Translation: AAA16400.1.
U00096 Genomic DNA. Translation: AAC75267.1.
AP009048 Genomic DNA. Translation: BAA15990.1.

PIR

E64990.

RefSeq

AP_002803.1.
NP_416711.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JSX	NMR	-	A	1-87	[»]
2PQ4	NMR	-	A	1-87	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-46322N.

IntAct

P0A9I5. 19 interactions.

STRING

P0A9I5.

Genome annotation databases

GeneID

945187.

GenomeReviews

Gene locus JW2195 in contig AP009048_GR.
Gene locus b2207 in contig U00096_GR.

KEGG

ecj:JW2195.
eco:b2207.

Organism-specific databases

EchoBASE

EB2064.

EcoGene

EG12143. napD.

CMR

Search...

Phylogenomic databases

HOGENOM

HBG466530.

OMA

IHAVTDE.

Enzyme and pathway databases

BioCyc

EcoCyc:NAPD-MON.
ECOL168927:B2207-MON.

Gene expression databases

Genevestigator

P0A9I5.

Family and domain databases

InterPro

IPR005623. NapD.
[Graphical view]

Pfam

PF03927. NapD. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

NAPD_ECOLI

Accession

Primary (citable) accession number: P0A9I5
Secondary accession number(s): P33938

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	December 15, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)