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Protein

Glycine cleavage system transcriptional repressor

Gene

gcvR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Negative transcriptional regulator of the glycine cleavage system operon (GCV). Does not autoregulate its own expression. It is not yet known how GcvR acts as a repressor. It does not seem to bind DNA. It could interact with GcvA and suppress its activatory activity.

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG11149-MONOMER.
ECOL316407:JW2464-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine cleavage system transcriptional repressor
Alternative name(s):
Gcv operon repressor
Gene namesi
Name:gcvR
Synonyms:yffD
Ordered Locus Names:b2479, JW2464
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11149. gcvR.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 190189Glycine cleavage system transcriptional repressorPRO_0000087448Add
BLAST

Proteomic databases

EPDiP0A9I3.
PaxDbiP0A9I3.
PRIDEiP0A9I3.

Interactioni

Protein-protein interaction databases

BioGridi4260580. 8 interactions.
DIPiDIP-9754N.
IntActiP0A9I3. 3 interactions.
STRINGi511145.b2479.

Structurei

3D structure databases

ProteinModelPortaliP0A9I3.
SMRiP0A9I3. Positions 4-178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 9182ACT 1PROSITE-ProRule annotationAdd
BLAST
Domaini97 – 17680ACT 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ACT domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105RH4. Bacteria.
COG2716. LUCA.
HOGENOMiHOG000272598.
InParanoidiP0A9I3.
KOiK03567.
OMAiDLITIMK.
OrthoDBiEOG65F8X8.
PhylomeDBiP0A9I3.

Family and domain databases

InterProiIPR002912. ACT_dom.
IPR016867. GcvR.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
[Graphical view]
PIRSFiPIRSF028103. GcvR. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9I3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLSSQHYLV ITALGADRPG IVNTITRHVS SCGCNIEDSR LAMLGEEFTF
60 70 80 90 100
IMLLSGSWNA ITLIESTLPL KGAELDLLIV MKRTTARPRP PMPASVWVQV
110 120 130 140 150
DVADSPHLIE RFTALFDAHH MNIAELVSRT QPAENERAAQ LHIQITAHSP
160 170 180 190
ASADAANIEQ AFKALCTELN AQGSINVVNY SQHDEQDGVK
Length:190
Mass (Da):20,769
Last modified:January 23, 2007 - v2
Checksum:i66A1998E767B556B
GO

Sequence cautioni

The sequence AAB88561.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023337 Genomic DNA. Translation: AAC46232.1.
U00096 Genomic DNA. Translation: AAC75532.2.
AP009048 Genomic DNA. Translation: BAA16356.2.
M63654 Genomic DNA. Translation: AAB88561.1. Different initiation.
RefSeqiNP_416974.4. NC_000913.3.
WP_000176187.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75532; AAC75532; b2479.
BAA16356; BAA16356; BAA16356.
GeneIDi946950.
KEGGiecj:JW2464.
eco:b2479.
PATRICi32120345. VBIEscCol129921_2575.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023337 Genomic DNA. Translation: AAC46232.1.
U00096 Genomic DNA. Translation: AAC75532.2.
AP009048 Genomic DNA. Translation: BAA16356.2.
M63654 Genomic DNA. Translation: AAB88561.1. Different initiation.
RefSeqiNP_416974.4. NC_000913.3.
WP_000176187.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A9I3.
SMRiP0A9I3. Positions 4-178.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260580. 8 interactions.
DIPiDIP-9754N.
IntActiP0A9I3. 3 interactions.
STRINGi511145.b2479.

Proteomic databases

EPDiP0A9I3.
PaxDbiP0A9I3.
PRIDEiP0A9I3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75532; AAC75532; b2479.
BAA16356; BAA16356; BAA16356.
GeneIDi946950.
KEGGiecj:JW2464.
eco:b2479.
PATRICi32120345. VBIEscCol129921_2575.

Organism-specific databases

EchoBASEiEB1138.
EcoGeneiEG11149. gcvR.

Phylogenomic databases

eggNOGiENOG4105RH4. Bacteria.
COG2716. LUCA.
HOGENOMiHOG000272598.
InParanoidiP0A9I3.
KOiK03567.
OMAiDLITIMK.
OrthoDBiEOG65F8X8.
PhylomeDBiP0A9I3.

Enzyme and pathway databases

BioCyciEcoCyc:EG11149-MONOMER.
ECOL316407:JW2464-MONOMER.

Miscellaneous databases

PROiP0A9I3.

Family and domain databases

InterProiIPR002912. ACT_dom.
IPR016867. GcvR.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
[Graphical view]
PIRSFiPIRSF028103. GcvR. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Promoter characterization and constitutive expression of the Escherichia coli gcvR gene."
    Ghrist A.C., Stauffer G.V.
    J. Bacteriol. 180:1803-1807(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-15, CHARACTERIZATION.
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A molecular analysis of the 53.3 minute region of the Escherichia coli linkage map."
    Andrews S.C., Harrison P.M., Guest J.R.
    J. Gen. Microbiol. 137:361-367(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 11-190.
    Strain: K12.
  6. Andrews S.C.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO C-TERMINUS.

Entry informationi

Entry nameiGCVR_ECOLI
AccessioniPrimary (citable) accession number: P0A9I3
Secondary accession number(s): O30979, P23483, P77652
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: April 13, 2016
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.