P0A9I3 (GCVR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine cleavage system transcriptional repressor

Alternative name(s):
Gcv operon repressor

Gene names

Name:	gcvR
Synonyms:	yffD
Ordered Locus Names:	b2479, JW2464

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negative transcriptional regulator of the glycine cleavage system operon (GCV). Does not autoregulate its own expression. It is not yet known how GcvR acts as a repressor. It does not seem to bind DNA. It could interact with GcvA and suppress its activatory activity.
Subcellular location	Cytoplasm.
Sequence caution	The sequence AAB88561.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Molecular function	Repressor
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from direct assay PubMed 12101307. Source: EcoCyc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	amino acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 190	189	Glycine cleavage system transcriptional repressor		PRO_0000087448

Sequences

	Sequence		Length	Mass (Da)
	P0A9I3 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 66A1998E767B556B	FASTA	190	20,769
	10 20 30 40 50 60 MTLSSQHYLV ITALGADRPG IVNTITRHVS SCGCNIEDSR LAMLGEEFTF IMLLSGSWNA 70 80 90 100 110 120 ITLIESTLPL KGAELDLLIV MKRTTARPRP PMPASVWVQV DVADSPHLIE RFTALFDAHH 130 140 150 160 170 180 MNIAELVSRT QPAENERAAQ LHIQITAHSP ASADAANIEQ AFKALCTELN AQGSINVVNY 190 SQHDEQDGVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Promoter characterization and constitutive expression of the Escherichia coli gcvR gene." Ghrist A.C., Stauffer G.V. J. Bacteriol. 180:1803-1807(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-15, CHARACTERIZATION. Strain: K12.
[2]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T. DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"A molecular analysis of the 53.3 minute region of the Escherichia coli linkage map." Andrews S.C., Harrison P.M., Guest J.R. J. Gen. Microbiol. 137:361-367(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 11-190. Strain: K12.
[6]	Andrews S.C. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO C-TERMINUS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF023337 Genomic DNA. Translation: AAC46232.1. U00096 Genomic DNA. Translation: AAC75532.2. AP009048 Genomic DNA. Translation: BAA16356.2. M63654 Genomic DNA. Translation: AAB88561.1. Different initiation.
RefSeq	NP_416974.4. NC_000913.2. YP_490707.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A9I3.
SMR	P0A9I3. Positions 4-178.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b2479.
Proteomic databases
PaxDb	P0A9I3.
PRIDE	P0A9I3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75532; AAC75532; b2479. BAA16356; BAA16356; BAA16356.
GeneID	12931586. 946950.
KEGG	ecj:Y75_p2432. eco:b2479.
PATRIC	32120345. VBIEscCol129921_2575.
Organism-specific databases
EchoBASE	EB1138.
EcoGene	EG11149. gcvR.
Phylogenomic databases
eggNOG	COG2716.
HOGENOM	HOG000272598.
KO	K03567.
OMA	VADSPHI.
ProtClustDB	PRK11589.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11149-MONOMER. ECOL316407:JW2464-MONOMER.
Gene expression databases
Genevestigator	P0A9I3.
Family and domain databases
InterPro	IPR002912. ACT_dom. IPR016867. Gly_cleavage_repressor_GcvR. [Graphical view]
Pfam	PF01842. ACT. 1 hit. [Graphical view]
PIRSF	PIRSF028103. GcvR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCVR_ECOLI

Accession

Primary (citable) accession number: P0A9I3
Secondary accession number(s): O30979, P23483, P77652

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program