ID   CITE_ECOLI              Reviewed;         302 AA.
AC   P0A9I1; O54335; P77770;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   25-JAN-2012, entry version 56.
DE   RecName: Full=Citrate lyase subunit beta;
DE            Short=Citrase beta chain;
DE            EC=4.1.3.6;
DE   AltName: Full=Citrate (pro-3S)-lyase subunit beta;
DE   AltName: Full=Citryl-CoA lyase subunit;
DE            EC=4.1.3.34;
GN   Name=citE; Synonyms=ybdW; OrderedLocusNames=b0616, JW0608;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   MEDLINE=97061202; PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A.,
RA   Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K.,
RA   Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K.,
RA   Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N.,
RA   Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y.,
RA   Yano M., Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome
RT   corresponding to the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J.,
RA   Mau B., Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1474(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains
RT   MG1655 and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-156.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Ingmer H., Cohen S.N.;
RL   Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Represents a citryl-ACP lyase (By similarity).
CC   -!- CATALYTIC ACTIVITY: Citrate = acetate + oxaloacetate.
CC   -!- CATALYTIC ACTIVITY: (3S)-citryl-CoA = acetyl-CoA + oxaloacetate.
CC   -!- COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
CC   -!- SUBUNIT: Oligomer with a subunit composition of (alpha,beta,
CC       gamma)6 (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the HpcH/HpaI aldolase family. Citrate
CC       lyase beta subunit subfamily.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB40816.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
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DR   EMBL; U82598; AAB40816.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U00096; AAC73717.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35252.1; -; Genomic_DNA.
DR   EMBL; U46667; AAC28948.1; -; Genomic_DNA.
DR   PIR; F64795; F64795.
DR   RefSeq; NP_415149.4; NC_000913.2.
DR   ProteinModelPortal; P0A9I1; -.
DR   SMR; P0A9I1; 10-245.
DR   IntAct; P0A9I1; 1.
DR   MINT; MINT-1283346; -.
DR   PRIDE; P0A9I1; -.
DR   EnsemblBacteria; EBESCT00000005021; EBESCP00000005021; EBESCG00000004097.
DR   EnsemblBacteria; EBESCT00000014845; EBESCP00000014136; EBESCG00000013905.
DR   GeneID; 945406; -.
DR   GenomeReviews; AP009048_GR; JW0608.
DR   GenomeReviews; U00096_GR; b0616.
DR   KEGG; ecj:JW0608; -.
DR   KEGG; eco:b0616; -.
DR   PATRIC; 32116412; VBIEscCol129921_0646.
DR   EchoBASE; EB3312; -.
DR   EcoGene; EG13542; citE.
DR   eggNOG; COG2301; -.
DR   GeneTree; EBGT00050000010557; -.
DR   HOGENOM; HBG676713; -.
DR   OMA; TDMKTHR; -.
DR   PhylomeDB; P0A9I1; -.
DR   ProtClustDB; CLSK874112; -.
DR   BioCyc; EcoCyc:CITRYLY-MONOMER; -.
DR   BioCyc; MetaCyc:CITRYLY-MONOMER; -.
DR   Genevestigator; P0A9I1; -.
DR   GO; GO:0009346; C:citrate lyase complex; IEA:InterPro.
DR   GO; GO:0008815; F:citrate (pro-3S)-lyase activity; IEA:EC.
DR   GO; GO:0008816; F:citryl-CoA lyase activity; IEA:EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006084; P:acetyl-CoA metabolic process; IEA:InterPro.
DR   GO; GO:0006725; P:cellular aromatic compound metabolic process; IEA:InterPro.
DR   InterPro; IPR005000; Aldehyde-lyase_domain.
DR   InterPro; IPR011206; Citrate_lyase_beta.
DR   InterPro; IPR006475; Citrate_lyase_beta_bac.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase.
DR   Gene3D; G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 1.
DR   KO; K01644; -.
DR   PANTHER; PTHR11105; PTHR11105; 1.
DR   Pfam; PF03328; HpcH_HpaI; 1.
DR   PIRSF; PIRSF015582; Cit_lyase_B; 1.
DR   SUPFAM; SSF51621; Pyrv/PenolPyrv_Kinase_cat; 1.
DR   TIGRFAMs; TIGR01588; CitE; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Lyase; Magnesium; Metal-binding;
KW   Reference proteome.
FT   CHAIN         1    302       Citrate lyase subunit beta.
FT                                /FTId=PRO_0000089757.
FT   METAL       139    139       Magnesium (By similarity).
FT   METAL       166    166       Magnesium (By similarity).
FT   BINDING      76     76       Substrate (By similarity).
FT   BINDING     139    139       Substrate (By similarity).
FT   CONFLICT    150    156       IAHASER -> SLTLPRL (in Ref. 5; AAC28948).
SQ   SEQUENCE   302 AA;  33110 MW;  204E8CCD40A2A52E CRC64;
     MISASLQQRK TRTRRSMLFV PGANAAMVSN SFIYPADALM FDLEDSVALR EKDTARRMVY
     HALQHPLYRD IETIVRVNAL DSEWGVNDLE AVVRGGADVV RLPKTDTAQD VLDIEKEILR
     IEKACGREPG STGLLAAIES PLGITRAVEI AHASERLIGI ALGAEDYVRN LRTERSPEGT
     ELLFARCSIL QAARSAGIQA FDTVYSDANN EAGFLQEAAH IKQLGFDGKS LINPRQIDLL
     HNLYAPTQKE VDHARRVVEA AEAAAREGLG VVSLNGKMVD GPVIDRARLV LSRAELSGIR
     EE
//