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Protein

Protein phosphatase CheZ

Gene

cheZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei147Enhances dephosphorylation of CheY-P1

GO - Molecular functioni

  • phosphoprotein phosphatase activity Source: EcoCyc

GO - Biological processi

  • bacterial-type flagellum-dependent swimming motility Source: EcoCyc
  • chemotaxis Source: UniProtKB-KW
  • regulation of chemotaxis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Chemotaxis, Flagellar rotation

Enzyme and pathway databases

BioCyciEcoCyc:CHEZ-MONOMER.
ECOL316407:JW1870-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase CheZ (EC:3.1.3.-)
Alternative name(s):
Chemotaxis protein CheZ
Gene namesi
Name:cheZ
Ordered Locus Names:b1881, JW1870
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10151. cheZ.

Subcellular locationi

GO - Cellular componenti

  • bacterial-type flagellum Source: InterPro
  • cytosol Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi21I → T: Gain of function, increased counterclockwise rotation of flagella. 1 Publication1
Mutagenesisi65A → V: Loss of function, fails to oligomerize. 1 Publication1
Mutagenesisi90L → S: Loss of localization to the cell pole. Fails to oligomerize. 2 Publications1
Mutagenesisi94W → R: Loss of localization to the cell pole. 1 Publication1
Mutagenesisi117F → S: Loss of localization to the cell pole. 2 Publications1
Mutagenesisi143D → G: Loss of function. Fails to oligomerize. 1 Publication1
Mutagenesisi147Q → A: Severely diminished function, exclusive clockwise flagellar rotation. 1 Publication1
Mutagenesisi188G → E: Diminished ability to stimulate dephosphorylation of CheY-P. 1 Publication1
Mutagenesisi205V → E: Severely impairs the interaction the interaction with CheY-P. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000896411 – 214Protein phosphatase CheZAdd BLAST214

Proteomic databases

PaxDbiP0A9H9.
PRIDEiP0A9H9.

2D gel databases

SWISS-2DPAGEP0A9H9.

Interactioni

Subunit structurei

Homodimer. Interacts with CheA isoform CheA(short). Interacts (via C-terminus) with phosphorylated CheY (CheY-P).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
cheAP073633EBI-546726,EBI-1026773
cheYP0AE673EBI-546726,EBI-546693

Protein-protein interaction databases

BioGridi4259554. 391 interactors.
DIPiDIP-9275N.
IntActiP0A9H9. 22 interactors.
MINTiMINT-1264225.
STRINGi511145.b1881.

Structurei

Secondary structure

1214
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 35Combined sources22
Helixi37 – 43Combined sources7
Turni44 – 47Combined sources4
Helixi48 – 54Combined sources7
Turni55 – 57Combined sources3
Helixi58 – 64Combined sources7
Turni65 – 67Combined sources3
Helixi68 – 97Combined sources30
Helixi104 – 138Combined sources35
Helixi141 – 161Combined sources21
Turni162 – 167Combined sources6
Helixi204 – 212Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KMIX-ray2.90Z1-214[»]
ProteinModelPortaliP0A9H9.
SMRiP0A9H9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9H9.

Family & Domainsi

Sequence similaritiesi

Belongs to the CheZ family.Curated

Phylogenomic databases

eggNOGiENOG4108KCC. Bacteria.
COG3143. LUCA.
HOGENOMiHOG000254724.
InParanoidiP0A9H9.
KOiK03414.
OMAiGPQIHAD.
PhylomeDBiP0A9H9.

Family and domain databases

InterProiIPR007439. Chemotax_Pase_CheZ.
[Graphical view]
PfamiPF04344. CheZ. 1 hit.
[Graphical view]
PIRSFiPIRSF002884. CheZ. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A9H9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMQPSIKPAD EHSAGDIIAR IGSLTRMLRD SLRELGLDQA IAEAAEAIPD
60 70 80 90 100
ARDRLYYVVQ MTAQAAERAL NSVEASQPHQ DQMEKSAKAL TQRWDDWFAD
110 120 130 140 150
PIDLADAREL VTDTRQFLAD VPAHTSFTNA QLLEIMMAQD FQDLTGQVIK
160 170 180 190 200
RMMDVIQEIE RQLLMVLLEN IPEQESRPKR ENQSLLNGPQ VDTSKAGVVA
210
SQDQVDDLLD SLGF
Length:214
Mass (Da):23,976
Last modified:April 1, 1988 - v1
Checksum:i42308F5AC0685D27
GO

Sequence cautioni

The sequence AAA23571 differs from that shown.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119A → P in AAA23571 (PubMed:3510184).Curated1
Sequence conflicti130A → G in AAA23571 (PubMed:3510184).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13463 Genomic DNA. Translation: AAA23571.1. Sequence problems.
U00096 Genomic DNA. Translation: AAC74951.1.
AP009048 Genomic DNA. Translation: BAA15697.1.
K02175 Genomic DNA. Translation: AAA23578.1.
PIRiF25195. QRECCZ.
RefSeqiNP_416395.1. NC_000913.3.
WP_000983609.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74951; AAC74951; b1881.
BAA15697; BAA15697; BAA15697.
GeneIDi946392.
KEGGiecj:JW1870.
eco:b1881.
PATRICi32119089. VBIEscCol129921_1962.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13463 Genomic DNA. Translation: AAA23571.1. Sequence problems.
U00096 Genomic DNA. Translation: AAC74951.1.
AP009048 Genomic DNA. Translation: BAA15697.1.
K02175 Genomic DNA. Translation: AAA23578.1.
PIRiF25195. QRECCZ.
RefSeqiNP_416395.1. NC_000913.3.
WP_000983609.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KMIX-ray2.90Z1-214[»]
ProteinModelPortaliP0A9H9.
SMRiP0A9H9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259554. 391 interactors.
DIPiDIP-9275N.
IntActiP0A9H9. 22 interactors.
MINTiMINT-1264225.
STRINGi511145.b1881.

2D gel databases

SWISS-2DPAGEP0A9H9.

Proteomic databases

PaxDbiP0A9H9.
PRIDEiP0A9H9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74951; AAC74951; b1881.
BAA15697; BAA15697; BAA15697.
GeneIDi946392.
KEGGiecj:JW1870.
eco:b1881.
PATRICi32119089. VBIEscCol129921_1962.

Organism-specific databases

EchoBASEiEB0149.
EcoGeneiEG10151. cheZ.

Phylogenomic databases

eggNOGiENOG4108KCC. Bacteria.
COG3143. LUCA.
HOGENOMiHOG000254724.
InParanoidiP0A9H9.
KOiK03414.
OMAiGPQIHAD.
PhylomeDBiP0A9H9.

Enzyme and pathway databases

BioCyciEcoCyc:CHEZ-MONOMER.
ECOL316407:JW1870-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A9H9.
PROiP0A9H9.

Family and domain databases

InterProiIPR007439. Chemotax_Pase_CheZ.
[Graphical view]
PfamiPF04344. CheZ. 1 hit.
[Graphical view]
PIRSFiPIRSF002884. CheZ. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCHEZ_ECOLI
AccessioniPrimary (citable) accession number: P0A9H9
Secondary accession number(s): P07366
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 2, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.