Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein phosphatase CheZ

Gene

cheZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in bacterial chemotaxis signal transduction pathway by accelerating the dephosphorylation of phosphorylated CheY (CheY-P).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei147Enhances dephosphorylation of CheY-P1

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • phosphoprotein phosphatase activity Source: EcoCyc

GO - Biological processi

  • bacterial-type flagellum-dependent swimming motility Source: EcoCyc
  • chemotaxis Source: UniProtKB-KW
  • regulation of chemotaxis Source: InterPro

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processChemotaxis, Flagellar rotation

Enzyme and pathway databases

BioCyciEcoCyc:CHEZ-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase CheZ (EC:3.1.3.-)
Alternative name(s):
Chemotaxis protein CheZ
Gene namesi
Name:cheZ
Ordered Locus Names:b1881, JW1870
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi

Organism-specific databases

EcoGeneiEG10151 cheZ

Subcellular locationi

GO - Cellular componenti

  • bacterial-type flagellum Source: InterPro
  • cytosol Source: EcoCyc
  • methyl accepting chemotaxis protein complex Source: CAFA
  • plasma membrane Source: CAFA

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi21I → T: Gain of function, increased counterclockwise rotation of flagella. 1 Publication1
Mutagenesisi65A → V: Loss of function, fails to oligomerize. 1 Publication1
Mutagenesisi90L → S: Loss of localization to the cell pole. Fails to oligomerize. 2 Publications1
Mutagenesisi94W → R: Loss of localization to the cell pole. 1 Publication1
Mutagenesisi117F → S: Loss of localization to the cell pole. 2 Publications1
Mutagenesisi143D → G: Loss of function. Fails to oligomerize. 1 Publication1
Mutagenesisi147Q → A: Severely diminished function, exclusive clockwise flagellar rotation. 1 Publication1
Mutagenesisi188G → E: Diminished ability to stimulate dephosphorylation of CheY-P. 1 Publication1
Mutagenesisi205V → E: Severely impairs the interaction the interaction with CheY-P. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000896411 – 214Protein phosphatase CheZAdd BLAST214

Proteomic databases

PaxDbiP0A9H9
PRIDEiP0A9H9

2D gel databases

SWISS-2DPAGEiP0A9H9

Interactioni

Subunit structurei

Homodimer. Interacts with CheA isoform CheA(short). Interacts (via C-terminus) with phosphorylated CheY (CheY-P).4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi4259554, 392 interactors
DIPiDIP-9275N
IntActiP0A9H9, 22 interactors
STRINGi316385.ECDH10B_2022

Structurei

Secondary structure

1214
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi14 – 35Combined sources22
Helixi37 – 43Combined sources7
Turni44 – 47Combined sources4
Helixi48 – 54Combined sources7
Turni55 – 57Combined sources3
Helixi58 – 64Combined sources7
Turni65 – 67Combined sources3
Helixi68 – 97Combined sources30
Helixi104 – 138Combined sources35
Helixi141 – 161Combined sources21
Turni162 – 167Combined sources6
Helixi204 – 212Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KMIX-ray2.90Z1-214[»]
ProteinModelPortaliP0A9H9
SMRiP0A9H9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9H9

Family & Domainsi

Sequence similaritiesi

Belongs to the CheZ family.Curated

Phylogenomic databases

eggNOGiENOG4108KCC Bacteria
COG3143 LUCA
HOGENOMiHOG000254724
InParanoidiP0A9H9
KOiK03414
OMAiGPQIHAD
PhylomeDBiP0A9H9

Family and domain databases

InterProiView protein in InterPro
IPR007439 Chemotax_Pase_CheZ
PfamiView protein in Pfam
PF04344 CheZ, 1 hit
PIRSFiPIRSF002884 CheZ, 1 hit

Sequencei

Sequence statusi: Complete.

P0A9H9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMQPSIKPAD EHSAGDIIAR IGSLTRMLRD SLRELGLDQA IAEAAEAIPD
60 70 80 90 100
ARDRLYYVVQ MTAQAAERAL NSVEASQPHQ DQMEKSAKAL TQRWDDWFAD
110 120 130 140 150
PIDLADAREL VTDTRQFLAD VPAHTSFTNA QLLEIMMAQD FQDLTGQVIK
160 170 180 190 200
RMMDVIQEIE RQLLMVLLEN IPEQESRPKR ENQSLLNGPQ VDTSKAGVVA
210
SQDQVDDLLD SLGF
Length:214
Mass (Da):23,976
Last modified:April 1, 1988 - v1
Checksum:i42308F5AC0685D27
GO

Sequence cautioni

The sequence AAA23571 differs from that shown.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti119A → P in AAA23571 (PubMed:3510184).Curated1
Sequence conflicti130A → G in AAA23571 (PubMed:3510184).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13463 Genomic DNA Translation: AAA23571.1 Sequence problems.
U00096 Genomic DNA Translation: AAC74951.1
AP009048 Genomic DNA Translation: BAA15697.1
K02175 Genomic DNA Translation: AAA23578.1
PIRiF25195 QRECCZ
RefSeqiNP_416395.1, NC_000913.3
WP_000983609.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74951; AAC74951; b1881
BAA15697; BAA15697; BAA15697
GeneIDi946392
KEGGiecj:JW1870
eco:b1881
PATRICifig|511145.12.peg.1962

Entry informationi

Entry nameiCHEZ_ECOLI
AccessioniPrimary (citable) accession number: P0A9H9
Secondary accession number(s): P07366
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health