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P0A9H7

- CFA_ECOLI

UniProt

P0A9H7 - CFA_ECOLI

Protein

Cyclopropane-fatty-acyl-phospholipid synthase

Gene

cfa

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.

    Enzyme regulationi

    Inhibited by sinefungin, A9145C and S-adenosyl-L-homocysteine.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei354 – 3541By similarity

    GO - Molecular functioni

    1. cyclopropane-fatty-acyl-phospholipid synthase activity Source: EcoCyc

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-UniPathway
    2. lipid modification Source: EcoCyc

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciEcoCyc:CFA-MONOMER.
    ECOL316407:JW1653-MONOMER.
    MetaCyc:CFA-MONOMER.
    RETL1328306-WGS:GSTH-906-MONOMER.
    SABIO-RKP0A9H7.
    UniPathwayiUPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclopropane-fatty-acyl-phospholipid synthase (EC:2.1.1.79)
    Short name:
    CFA synthase
    Short name:
    Cyclopropane fatty acid synthase
    Gene namesi
    Name:cfa
    Synonyms:cdfA
    Ordered Locus Names:b1661, JW1653
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11531. cfa.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 382381Cyclopropane-fatty-acyl-phospholipid synthasePRO_0000089572Add
    BLAST

    Proteomic databases

    PaxDbiP0A9H7.
    PRIDEiP0A9H7.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A9H7.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    hrpBP370241EBI-1124838,EBI-549226

    Protein-protein interaction databases

    DIPiDIP-48026N.
    IntActiP0A9H7. 2 interactions.
    MINTiMINT-1312967.
    STRINGi511145.b1661.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A9H7.
    SMRiP0A9H7. Positions 120-359.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni137 – 1382S-adenosyl-L-methionine bindingBy similarity
    Regioni171 – 1799S-adenosyl-L-methionine bindingBy similarity
    Regioni197 – 2026S-adenosyl-L-methionine bindingBy similarity

    Sequence similaritiesi

    Belongs to the CFA/CMAS family.Curated

    Phylogenomic databases

    eggNOGiCOG2230.
    HOGENOMiHOG000245194.
    KOiK00574.
    OMAiLMLDPYM.
    OrthoDBiEOG6BGNZP.
    PhylomeDBiP0A9H7.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR003333. Mycolic_cyclopropane_synthase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02353. CMAS. 1 hit.
    [Graphical view]
    PIRSFiPIRSF003085. CMAS. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0A9H7-1 [UniParc]FASTAAdd to Basket

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    MSSSCIEEVS VPDDNWYRIA NELLSRAGIA INGSAPADIR VKNPDFFKRV    50
    LQEGSLGLGE SYMDGWWECD RLDMFFSKVL RAGLENQLPH HFKDTLRIAG 100
    ARLFNLQSKK RAWIVGKEHY DLGNDLFSRM LDPFMQYSCA YWKDADNLES 150
    AQQAKLKMIC EKLQLKPGMR VLDIGCGWGG LAHYMASNYD VSVVGVTISA 200
    EQQKMAQERC EGLDVTILLQ DYRDLNDQFD RIVSVGMFEH VGPKNYDTYF 250
    AVVDRNLKPE GIFLLHTIGS KKTDLNVDPW INKYIFPNGC LPSVRQIAQS 300
    SEPHFVMEDW HNFGADYDTT LMAWYERFLA AWPEIADNYS ERFKRMFTYY 350
    LNACAGAFRA RDIQLWQVVF SRGVENGLRV AR 382
    Length:382
    Mass (Da):43,909
    Last modified:January 23, 2007 - v2
    Checksum:i861FF3314FDAF2C4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21S → R in X69109. 1 PublicationCurated
    Sequence conflicti8 – 81E → G in X69109. 1 PublicationCurated
    Sequence conflicti25 – 251S → N in X69109. 1 PublicationCurated
    Sequence conflicti39 – 391I → T in X69109. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98330 Genomic DNA. Translation: AAA23562.1.
    X69109 Genomic DNA. No translation available.
    U00096 Genomic DNA. Translation: AAC74733.1.
    AP009048 Genomic DNA. Translation: BAA15428.1.
    PIRiA44292.
    RefSeqiNP_416178.1. NC_000913.3.
    YP_489925.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74733; AAC74733; b1661.
    BAA15428; BAA15428; BAA15428.
    GeneIDi12934382.
    944811.
    KEGGiecj:Y75_p1638.
    eco:b1661.
    PATRICi32118628. VBIEscCol129921_1734.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98330 Genomic DNA. Translation: AAA23562.1 .
    X69109 Genomic DNA. No translation available.
    U00096 Genomic DNA. Translation: AAC74733.1 .
    AP009048 Genomic DNA. Translation: BAA15428.1 .
    PIRi A44292.
    RefSeqi NP_416178.1. NC_000913.3.
    YP_489925.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0A9H7.
    SMRi P0A9H7. Positions 120-359.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48026N.
    IntActi P0A9H7. 2 interactions.
    MINTi MINT-1312967.
    STRINGi 511145.b1661.

    Proteomic databases

    PaxDbi P0A9H7.
    PRIDEi P0A9H7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74733 ; AAC74733 ; b1661 .
    BAA15428 ; BAA15428 ; BAA15428 .
    GeneIDi 12934382.
    944811.
    KEGGi ecj:Y75_p1638.
    eco:b1661.
    PATRICi 32118628. VBIEscCol129921_1734.

    Organism-specific databases

    EchoBASEi EB1493.
    EcoGenei EG11531. cfa.

    Phylogenomic databases

    eggNOGi COG2230.
    HOGENOMi HOG000245194.
    KOi K00574.
    OMAi LMLDPYM.
    OrthoDBi EOG6BGNZP.
    PhylomeDBi P0A9H7.

    Enzyme and pathway databases

    UniPathwayi UPA00094 .
    BioCyci EcoCyc:CFA-MONOMER.
    ECOL316407:JW1653-MONOMER.
    MetaCyc:CFA-MONOMER.
    RETL1328306-WGS:GSTH-906-MONOMER.
    SABIO-RK P0A9H7.

    Miscellaneous databases

    PROi P0A9H7.

    Gene expression databases

    Genevestigatori P0A9H7.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR003333. Mycolic_cyclopropane_synthase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02353. CMAS. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF003085. CMAS. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cyclopropane fatty acid synthase of Escherichia coli: deduced amino acid sequence, purification, and studies of the enzyme active site."
      Wang A.-Y., Grogan D.W., Cronan J.E. Jr.
      Biochemistry 31:11020-11028(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-9, ENZYME REGULATION.
      Strain: K12.
    2. Eberhardt S.M.R., Richter G., Gimbel W., Werner T., Bacher A.
      Submitted (NOV-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / RR28.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiCFA_ECOLI
    AccessioniPrimary (citable) accession number: P0A9H7
    Secondary accession number(s): P30010
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3