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P0A9H7

- CFA_ECOLI

UniProt

P0A9H7 - CFA_ECOLI

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Protein

Cyclopropane-fatty-acyl-phospholipid synthase

Gene
cfa, cdfA, b1661, JW1653
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge By similarity.

Catalytic activityi

S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.

Enzyme regulationi

Inhibited by sinefungin, A9145C and S-adenosyl-L-homocysteine.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei354 – 3541 By similarity

GO - Molecular functioni

  1. cyclopropane-fatty-acyl-phospholipid synthase activity Source: EcoCyc

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-UniPathway
  2. lipid modification Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:CFA-MONOMER.
ECOL316407:JW1653-MONOMER.
MetaCyc:CFA-MONOMER.
RETL1328306-WGS:GSTH-906-MONOMER.
SABIO-RKP0A9H7.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclopropane-fatty-acyl-phospholipid synthase (EC:2.1.1.79)
Short name:
CFA synthase
Short name:
Cyclopropane fatty acid synthase
Gene namesi
Name:cfa
Synonyms:cdfA
Ordered Locus Names:b1661, JW1653
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11531. cfa.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 382381Cyclopropane-fatty-acyl-phospholipid synthasePRO_0000089572Add
BLAST

Proteomic databases

PaxDbiP0A9H7.
PRIDEiP0A9H7.

Expressioni

Gene expression databases

GenevestigatoriP0A9H7.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
hrpBP370241EBI-1124838,EBI-549226

Protein-protein interaction databases

DIPiDIP-48026N.
IntActiP0A9H7. 2 interactions.
MINTiMINT-1312967.
STRINGi511145.b1661.

Structurei

3D structure databases

ProteinModelPortaliP0A9H7.
SMRiP0A9H7. Positions 120-359.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 1382S-adenosyl-L-methionine binding By similarity
Regioni171 – 1799S-adenosyl-L-methionine binding By similarity
Regioni197 – 2026S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

Belongs to the CFA/CMAS family.

Phylogenomic databases

eggNOGiCOG2230.
HOGENOMiHOG000245194.
KOiK00574.
OMAiLMLDPYM.
OrthoDBiEOG6BGNZP.
PhylomeDBiP0A9H7.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003333. Mycolic_cyclopropane_synthase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02353. CMAS. 1 hit.
[Graphical view]
PIRSFiPIRSF003085. CMAS. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9H7-1 [UniParc]FASTAAdd to Basket

« Hide

MSSSCIEEVS VPDDNWYRIA NELLSRAGIA INGSAPADIR VKNPDFFKRV    50
LQEGSLGLGE SYMDGWWECD RLDMFFSKVL RAGLENQLPH HFKDTLRIAG 100
ARLFNLQSKK RAWIVGKEHY DLGNDLFSRM LDPFMQYSCA YWKDADNLES 150
AQQAKLKMIC EKLQLKPGMR VLDIGCGWGG LAHYMASNYD VSVVGVTISA 200
EQQKMAQERC EGLDVTILLQ DYRDLNDQFD RIVSVGMFEH VGPKNYDTYF 250
AVVDRNLKPE GIFLLHTIGS KKTDLNVDPW INKYIFPNGC LPSVRQIAQS 300
SEPHFVMEDW HNFGADYDTT LMAWYERFLA AWPEIADNYS ERFKRMFTYY 350
LNACAGAFRA RDIQLWQVVF SRGVENGLRV AR 382
Length:382
Mass (Da):43,909
Last modified:January 23, 2007 - v2
Checksum:i861FF3314FDAF2C4
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21S → R in X69109. 1 Publication
Sequence conflicti8 – 81E → G in X69109. 1 Publication
Sequence conflicti25 – 251S → N in X69109. 1 Publication
Sequence conflicti39 – 391I → T in X69109. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M98330 Genomic DNA. Translation: AAA23562.1.
X69109 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC74733.1.
AP009048 Genomic DNA. Translation: BAA15428.1.
PIRiA44292.
RefSeqiNP_416178.1. NC_000913.3.
YP_489925.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74733; AAC74733; b1661.
BAA15428; BAA15428; BAA15428.
GeneIDi12934382.
944811.
KEGGiecj:Y75_p1638.
eco:b1661.
PATRICi32118628. VBIEscCol129921_1734.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M98330 Genomic DNA. Translation: AAA23562.1 .
X69109 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC74733.1 .
AP009048 Genomic DNA. Translation: BAA15428.1 .
PIRi A44292.
RefSeqi NP_416178.1. NC_000913.3.
YP_489925.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0A9H7.
SMRi P0A9H7. Positions 120-359.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48026N.
IntActi P0A9H7. 2 interactions.
MINTi MINT-1312967.
STRINGi 511145.b1661.

Proteomic databases

PaxDbi P0A9H7.
PRIDEi P0A9H7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74733 ; AAC74733 ; b1661 .
BAA15428 ; BAA15428 ; BAA15428 .
GeneIDi 12934382.
944811.
KEGGi ecj:Y75_p1638.
eco:b1661.
PATRICi 32118628. VBIEscCol129921_1734.

Organism-specific databases

EchoBASEi EB1493.
EcoGenei EG11531. cfa.

Phylogenomic databases

eggNOGi COG2230.
HOGENOMi HOG000245194.
KOi K00574.
OMAi LMLDPYM.
OrthoDBi EOG6BGNZP.
PhylomeDBi P0A9H7.

Enzyme and pathway databases

UniPathwayi UPA00094 .
BioCyci EcoCyc:CFA-MONOMER.
ECOL316407:JW1653-MONOMER.
MetaCyc:CFA-MONOMER.
RETL1328306-WGS:GSTH-906-MONOMER.
SABIO-RK P0A9H7.

Miscellaneous databases

PROi P0A9H7.

Gene expression databases

Genevestigatori P0A9H7.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR003333. Mycolic_cyclopropane_synthase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02353. CMAS. 1 hit.
[Graphical view ]
PIRSFi PIRSF003085. CMAS. 1 hit.
SUPFAMi SSF53335. SSF53335. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cyclopropane fatty acid synthase of Escherichia coli: deduced amino acid sequence, purification, and studies of the enzyme active site."
    Wang A.-Y., Grogan D.W., Cronan J.E. Jr.
    Biochemistry 31:11020-11028(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-9, ENZYME REGULATION.
    Strain: K12.
  2. Eberhardt S.M.R., Richter G., Gimbel W., Werner T., Bacher A.
    Submitted (NOV-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / RR28.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiCFA_ECOLI
AccessioniPrimary (citable) accession number: P0A9H7
Secondary accession number(s): P30010
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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