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Protein

Cyclopropane-fatty-acyl-phospholipid synthase

Gene

cfa

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge.By similarity

Catalytic activityi

S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.

Enzyme regulationi

Inhibited by sinefungin, A9145C and S-adenosyl-L-homocysteine.1 Publication

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei354By similarity1

GO - Molecular functioni

  • cyclopropane-fatty-acyl-phospholipid synthase activity Source: EcoCyc

GO - Biological processi

  • fatty acid biosynthetic process Source: UniProtKB-UniPathway
  • lipid modification Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:CFA-MONOMER
MetaCyc:CFA-MONOMER
SABIO-RKiP0A9H7
UniPathwayiUPA00094

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclopropane-fatty-acyl-phospholipid synthase (EC:2.1.1.79)
Short name:
CFA synthase
Short name:
Cyclopropane fatty acid synthase
Gene namesi
Name:cfa
Synonyms:cdfA
Ordered Locus Names:b1661, JW1653
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11531 cfa

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000895722 – 382Cyclopropane-fatty-acyl-phospholipid synthaseAdd BLAST381

Proteomic databases

PaxDbiP0A9H7
PRIDEiP0A9H7

Interactioni

Protein-protein interaction databases

BioGridi4263376, 307 interactors
DIPiDIP-48026N
IntActiP0A9H7, 4 interactors
STRINGi316385.ECDH10B_1795

Structurei

3D structure databases

ProteinModelPortaliP0A9H7
SMRiP0A9H7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni137 – 138S-adenosyl-L-methionine bindingBy similarity2
Regioni171 – 179S-adenosyl-L-methionine bindingBy similarity9
Regioni197 – 202S-adenosyl-L-methionine bindingBy similarity6

Sequence similaritiesi

Belongs to the CFA/CMAS family.Curated

Phylogenomic databases

eggNOGiENOG4105CV9 Bacteria
COG2230 LUCA
HOGENOMiHOG000245194
InParanoidiP0A9H7
KOiK00574
OMAiLHCITAQ
PhylomeDBiP0A9H7

Family and domain databases

InterProiView protein in InterPro
IPR003333 Mycolic_cyclopropane_synthase
IPR029063 SAM-dependent_MTases
PIRSFiPIRSF003085 CMAS, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A9H7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSCIEEVS VPDDNWYRIA NELLSRAGIA INGSAPADIR VKNPDFFKRV
60 70 80 90 100
LQEGSLGLGE SYMDGWWECD RLDMFFSKVL RAGLENQLPH HFKDTLRIAG
110 120 130 140 150
ARLFNLQSKK RAWIVGKEHY DLGNDLFSRM LDPFMQYSCA YWKDADNLES
160 170 180 190 200
AQQAKLKMIC EKLQLKPGMR VLDIGCGWGG LAHYMASNYD VSVVGVTISA
210 220 230 240 250
EQQKMAQERC EGLDVTILLQ DYRDLNDQFD RIVSVGMFEH VGPKNYDTYF
260 270 280 290 300
AVVDRNLKPE GIFLLHTIGS KKTDLNVDPW INKYIFPNGC LPSVRQIAQS
310 320 330 340 350
SEPHFVMEDW HNFGADYDTT LMAWYERFLA AWPEIADNYS ERFKRMFTYY
360 370 380
LNACAGAFRA RDIQLWQVVF SRGVENGLRV AR
Length:382
Mass (Da):43,909
Last modified:January 23, 2007 - v2
Checksum:i861FF3314FDAF2C4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2S → R in X69109 (Ref. 2) Curated1
Sequence conflicti8E → G in X69109 (Ref. 2) Curated1
Sequence conflicti25S → N in X69109 (Ref. 2) Curated1
Sequence conflicti39I → T in X69109 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98330 Genomic DNA Translation: AAA23562.1
X69109 Genomic DNA No translation available.
U00096 Genomic DNA Translation: AAC74733.1
AP009048 Genomic DNA Translation: BAA15428.1
PIRiA44292
RefSeqiNP_416178.1, NC_000913.3
WP_000098896.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74733; AAC74733; b1661
BAA15428; BAA15428; BAA15428
GeneIDi944811
KEGGiecj:JW1653
eco:b1661
PATRICifig|1411691.4.peg.596

Similar proteinsi

Entry informationi

Entry nameiCFA_ECOLI
AccessioniPrimary (citable) accession number: P0A9H7
Secondary accession number(s): P30010
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 100 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health