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P0A9H5 (BTUR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cob(I)yrinic acid a,c-diamide adenosyltransferase

EC=2.5.1.17
Alternative name(s):
Cob(I)alamin adenosyltransferase
Corrinoid adenosyltransferase
Gene names
Name:btuR
Synonyms:cobA
Ordered Locus Names:b1270, JW1262
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length196 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for both de novo synthesis of the corrin ring for the assimilation of exogenous corrinoids. Participates in the adenosylation of a variety of incomplete and complete corrinoids By similarity.

Catalytic activity

ATP + cob(I)yrinic acid a,c-diamide = triphosphate + adenosylcob(III)yrinic acid a,c-diamide.

ATP + cobinamide = triphosphate + adenosylcobinamide.

Pathway

Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 2/7.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the Cob(I)alamin adenosyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 196196Cob(I)yrinic acid a,c-diamide adenosyltransferase
PRO_0000065009

Regions

Nucleotide binding36 – 427ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A9H5 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: 4EE2FDD9C74FE8AE

FASTA19621,999
        10         20         30         40         50         60 
MSDERYQQRQ QRVKEKVDAR VAQAQDERGI IIVFTGNGKG KTTAAFGTAT RAVGHGKKVG 

        70         80         90        100        110        120 
VVQFIKGTWP NGERNLLEPH GVEFQVMATG FTWDTQNRES DTAACREVWQ HAKRMLADSS 

       130        140        150        160        170        180 
LDMVLLDELT YMVAYDYLPL EEVVQALNER PHQQTVIITG RGCHRDILEL ADTVSELRPV 

       190 
KHAFDAGVKA QIGIDY 

« Hide

References

« Hide 'large scale' references
[1]"Altered cobalamin metabolism in Escherichia coli btuR mutants affects btuB gene regulation."
Lundrigan M.D., Kadner R.J.
J. Bacteriol. 171:154-161(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M21528 Genomic DNA. Translation: AAA23530.1.
U00096 Genomic DNA. Translation: AAC74352.1.
AP009048 Genomic DNA. Translation: BAA14807.1.
PIRA64875.
RefSeqNP_415786.1. NC_000913.3.
YP_489538.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0A9H5.
SMRP0A9H5. Positions 28-196.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP0A9H5. 14 interactions.
STRING511145.b1270.

Proteomic databases

PaxDbP0A9H5.
PRIDEP0A9H5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74352; AAC74352; b1270.
BAA14807; BAA14807; BAA14807.
GeneID12930581.
945839.
KEGGecj:Y75_p1244.
eco:b1270.
PATRIC32117798. VBIEscCol129921_1319.

Organism-specific databases

EchoBASEEB0128.
EcoGeneEG10130. btuR.

Phylogenomic databases

eggNOGCOG2109.
HOGENOMHOG000260311.
KOK00798.
OMAPVKHAFE.
OrthoDBEOG6GN76J.
PhylomeDBP0A9H5.

Enzyme and pathway databases

BioCycEcoCyc:COBALADENOSYLTRANS-MONOMER.
ECOL316407:JW1262-MONOMER.
UniPathwayUPA00148; UER00233.

Gene expression databases

GenevestigatorP0A9H5.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003724. AdoCbl_synth_CblAdoTrfase_CobA.
IPR025826. Co_AT_N_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF12557. Co_AT_N. 1 hit.
PF02572. CobA_CobO_BtuR. 1 hit.
[Graphical view]
PIRSFPIRSF015617. Adensltrnsf_CobA. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00708. cobA. 1 hit.
ProtoNetSearch...

Other

PROP0A9H5.

Entry information

Entry nameBTUR_ECOLI
AccessionPrimary (citable) accession number: P0A9H5
Secondary accession number(s): P13040
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene