P0A9H2 (MUG_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
G/U mismatch-specific DNA glycosylase
EC=3.2.2.28

Alternative name(s):
Double-strand-specific uracil glycosylase
Mismatch-specific uracil DNA-glycosylase
Short name=MUG

Gene names

Name:	mug
Ordered Locus Names:	c3822

Organism

Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

199310 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	168 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Excises ethenocytosine and uracil, which can arise by alkylation or deamination of cytosine, respectively, from the corresponding mispairs with guanine in ds-DNA. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Required for DNA damage lesion repair in stationary-phase cells By similarity. HAMAP-Rule MF_01956
Catalytic activity	Specifically hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free uracil. HAMAP-Rule MF_01956
Subunit structure	Binds DNA as a monomer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01956.
Sequence similarities	Belongs to the TDG/mug DNA glycosylase family.

Ontologies

Keywords
Biological process	DNA damage DNA excision DNA repair
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	base-excision repair, AP site formation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine-specific mismatch base pair DNA N-glycosylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 168	168	G/U mismatch-specific DNA glycosylase HAMAP-Rule MF_01956		PRO_0000185775

Sequences

	Sequence		Length	Mass (Da)
	P0A9H2 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: F1CD9B2E2030D6A7	FASTA	168	18,673
	10 20 30 40 50 60 MVEDILAPGL RVVFCGINPG LSSAGTGFPF AHPANRFWKV IYQAGFTDRQ LKPQEAQHLL 70 80 90 100 110 120 DYRCGVTKLV DRPTVQANEV SKQELHAGGR KLIEKIEDYQ PQALAILGKQ AYEQGFSQRG 130 140 150 160 AQWGKQTLTI GSTQIWVLPN PSGLSRVSLE KLVEAYRELD QALVVRGR

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014075 Genomic DNA. Translation: AAN82267.1.
RefSeq	NP_755693.1. NC_004431.1.
3D structure databases
ProteinModelPortal	P0A9H2.
SMR	P0A9H2. Positions 1-165.
ModBase	Search...
Protein-protein interaction databases
STRING	199310.c3822.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN82267; AAN82267; c3822.
GeneID	1039976.
KEGG	ecc:c3822.
PATRIC	18285435. VBIEscCol75197_3601.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000264684.
KO	K03649.
OMA	FARPGNR.
ProtClustDB	PRK10201.
Family and domain databases
Gene3D	3.40.470.10. 1 hit.
HAMAP	MF_01956. MUG.
InterPro	IPR023502. DNA_glyclase_G/T-mismatch_bac. IPR015637. DNA_glycosylase_G/T-mismatch. IPR005122. Uracil-DNA_glycosylase-like. [Graphical view]
PANTHER	PTHR12159. PTHR12159. 1 hit.
Pfam	PF03167. UDG. 1 hit. [Graphical view]
SMART	SM00986. UDG. 1 hit. [Graphical view]
SUPFAM	SSF52141. UDNA_glycsylseSF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MUG_ECOL6

Accession

Primary (citable) accession number: P0A9H2
Secondary accession number(s): P43342

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program