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P0A9H1

- MUG_ECOLI

UniProt

P0A9H1 - MUG_ECOLI

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Protein

G/U mismatch-specific DNA glycosylase

Gene
mug, ygjF, b3068, JW3040
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Excises ethenocytosine and uracil, which can arise by alkylation or deamination of cytosine, respectively, from the corresponding mispairs with guanine in ds-DNA. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Required for DNA damage lesion repair in stationary-phase cells.2 Publications

Catalytic activityi

Specifically hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free uracil.UniRule annotation

Enzyme regulationi

Subject to strong product inhibition. N-glycosyl hydrolysis proceeds 100-fold faster than product release.UniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. pyrimidine-specific mismatch base pair DNA N-glycosylase activity Source: EcoCyc

GO - Biological processi

  1. base-excision repair, AP site formation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

DNA damage, DNA excision, DNA repair

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12717-MONOMER.
ECOL316407:JW3040-MONOMER.
MetaCyc:EG12717-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
G/U mismatch-specific DNA glycosylase (EC:3.2.2.28)
Alternative name(s):
Double-strand-specific uracil glycosylase
Mismatch-specific uracil DNA-glycosylase
Short name:
MUG
Gene namesi
Name:mug
Synonyms:ygjF
Ordered Locus Names:b3068, JW3040
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12717. mug.

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 168168G/U mismatch-specific DNA glycosylaseUniRule annotationPRO_0000185774Add
BLAST

Proteomic databases

PaxDbiP0A9H1.
PRIDEiP0A9H1.

Expressioni

Inductioni

Induced in stationary phase.1 Publication

Gene expression databases

GenevestigatoriP0A9H1.

Interactioni

Subunit structurei

Binds DNA as a monomer.

Protein-protein interaction databases

IntActiP0A9H1. 21 interactions.
STRINGi511145.b3068.

Structurei

Secondary structure

1
168
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 188
Helixi21 – 266
Helixi37 – 437
Beta strandi46 – 494
Helixi53 – 6210
Beta strandi64 – 696
Beta strandi74 – 763
Helixi77 – 793
Helixi82 – 9918
Beta strandi102 – 1076
Helixi109 – 1168
Beta strandi123 – 1308
Beta strandi133 – 1386
Helixi149 – 16214

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1MTLX-ray2.80A/B1-168[»]
1MUGX-ray1.80A1-168[»]
1MWIX-ray2.35A1-168[»]
1MWJX-ray2.85A1-168[»]
ProteinModelPortaliP0A9H1.
SMRiP0A9H1. Positions 1-165.

Miscellaneous databases

EvolutionaryTraceiP0A9H1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3663.
HOGENOMiHOG000264684.
KOiK03649.
OMAiETGCGIT.
OrthoDBiEOG68H872.
PhylomeDBiP0A9H1.

Family and domain databases

Gene3Di3.40.470.10. 1 hit.
HAMAPiMF_01956. MUG.
InterProiIPR023502. DNA_glyclase_G/T-mismatch_bac.
IPR015637. DNA_glycosylase_G/T-mismatch.
IPR005122. Uracil-DNA_glycosylase-like.
[Graphical view]
PANTHERiPTHR12159. PTHR12159. 1 hit.
PfamiPF03167. UDG. 1 hit.
[Graphical view]
SMARTiSM00986. UDG. 1 hit.
[Graphical view]
SUPFAMiSSF52141. SSF52141. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A9H1-1 [UniParc]FASTAAdd to Basket

« Hide

MVEDILAPGL RVVFCGINPG LSSAGTGFPF AHPANRFWKV IYQAGFTDRQ    50
LKPQEAQHLL DYRCGVTKLV DRPTVQANEV SKQELHAGGR KLIEKIEDYQ 100
PQALAILGKQ AYEQGFSQRG AQWGKQTLTI GSTQIWVLPN PSGLSRVSLE 150
KLVEAYRELD QALVVRGR 168
Length:168
Mass (Da):18,673
Last modified:July 19, 2005 - v1
Checksum:iF1CD9B2E2030D6A7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U28379 Genomic DNA. Translation: AAA89148.1.
U00096 Genomic DNA. Translation: AAC76104.1.
AP009048 Genomic DNA. Translation: BAE77119.1.
J01687 Genomic DNA. No translation available.
PIRiB65095.
RefSeqiNP_417540.1. NC_000913.3.
YP_491260.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76104; AAC76104; b3068.
BAE77119; BAE77119; BAE77119.
GeneIDi12933393.
947560.
KEGGiecj:Y75_p2994.
eco:b3068.
PATRICi32121552. VBIEscCol129921_3163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U28379 Genomic DNA. Translation: AAA89148.1 .
U00096 Genomic DNA. Translation: AAC76104.1 .
AP009048 Genomic DNA. Translation: BAE77119.1 .
J01687 Genomic DNA. No translation available.
PIRi B65095.
RefSeqi NP_417540.1. NC_000913.3.
YP_491260.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1MTL X-ray 2.80 A/B 1-168 [» ]
1MUG X-ray 1.80 A 1-168 [» ]
1MWI X-ray 2.35 A 1-168 [» ]
1MWJ X-ray 2.85 A 1-168 [» ]
ProteinModelPortali P0A9H1.
SMRi P0A9H1. Positions 1-165.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P0A9H1. 21 interactions.
STRINGi 511145.b3068.

Proteomic databases

PaxDbi P0A9H1.
PRIDEi P0A9H1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76104 ; AAC76104 ; b3068 .
BAE77119 ; BAE77119 ; BAE77119 .
GeneIDi 12933393.
947560.
KEGGi ecj:Y75_p2994.
eco:b3068.
PATRICi 32121552. VBIEscCol129921_3163.

Organism-specific databases

EchoBASEi EB2576.
EcoGenei EG12717. mug.

Phylogenomic databases

eggNOGi COG3663.
HOGENOMi HOG000264684.
KOi K03649.
OMAi ETGCGIT.
OrthoDBi EOG68H872.
PhylomeDBi P0A9H1.

Enzyme and pathway databases

BioCyci EcoCyc:EG12717-MONOMER.
ECOL316407:JW3040-MONOMER.
MetaCyc:EG12717-MONOMER.

Miscellaneous databases

EvolutionaryTracei P0A9H1.
PROi P0A9H1.

Gene expression databases

Genevestigatori P0A9H1.

Family and domain databases

Gene3Di 3.40.470.10. 1 hit.
HAMAPi MF_01956. MUG.
InterProi IPR023502. DNA_glyclase_G/T-mismatch_bac.
IPR015637. DNA_glycosylase_G/T-mismatch.
IPR005122. Uracil-DNA_glycosylase-like.
[Graphical view ]
PANTHERi PTHR12159. PTHR12159. 1 hit.
Pfami PF03167. UDG. 1 hit.
[Graphical view ]
SMARTi SM00986. UDG. 1 hit.
[Graphical view ]
SUPFAMi SSF52141. SSF52141. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E coli K12."
    Burton Z.F., Burgess R.R., Lin J., Moore D., Holder S., Gross C.A.
    Nucleic Acids Res. 9:2889-2903(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 118-168.
    Strain: K12.
  4. "Detection of new genes in a bacterial genome using Markov models for three gene classes."
    Borodovsky M., McIninch J., Koonin E.V., Rudd K.E., Medigue C., Danchin A.
    Nucleic Acids Res. 23:3554-3562(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  5. "A new class of uracil-DNA glycosylases related to human thymine-DNA glycosylase."
    Gallinari P., Jiricny J.
    Nature 383:735-738(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Escherichia coli DNA glycosylase Mug: a growth-regulated enzyme required for mutation avoidance in stationary-phase cells."
    Mokkapati S.K., Fernandez de Henestrosa A.R., Bhagwat A.S.
    Mol. Microbiol. 41:1101-1111(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Mismatch uracil glycosylase from Escherichia coli: a general mismatch or a specific DNA glycosylase?"
    O'Neill R.J., Vorob'eva O.V., Shahbakhti H., Zmuda E., Bhagwat A.S., Baldwin G.S.
    J. Biol. Chem. 278:20526-20532(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Crystal structure of a G:T/U mismatch-specific DNA glycosylase: mismatch recognition by complementary-strand interactions."
    Barrett T.E., Savva R., Panayotou G., Brown T., Barlow T., Jiricny J., Pearl L.H.
    Cell 92:117-129(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH DNA.
  9. "Structure of a DNA base-excision product resembling a cisplatin inter-strand adduct."
    Barrett T.E., Savva R., Barlow T., Brown T., Jiricny J., Pearl L.H.
    Nat. Struct. Biol. 5:697-701(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) IN COMPLEX WITH DNA.
  10. "Crystal structure of a thwarted mismatch glycosylase DNA repair complex."
    Barrett T.E., Schaerer O.D., Savva R., Brown T., Jiricny J., Verdine G.L., Pearl L.H.
    EMBO J. 18:6599-6609(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS) IN COMPLEX WITH DNA.

Entry informationi

Entry nameiMUG_ECOLI
AccessioniPrimary (citable) accession number: P0A9H1
Secondary accession number(s): P43342, Q2M9D7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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