P0A9G2 (NHAR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional activator protein NhaR

Alternative name(s):
Na(+)/H(+) antiporter regulatory protein

Gene names

Name:	nhaR
Synonyms:	antO
Ordered Locus Names:	b0020, JW0019

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	301 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the positive regulation of NhaA. Ref.7
Subcellular location	Cytoplasm.
Sequence similarities	Contains 1 HTH lysR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from expression pattern Ref.7. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 301	301	Transcriptional activator protein NhaR		PRO_0000105689
Regions
	Domain	6 – 63	58	HTH lysR-type
	DNA binding	23 – 42	20	H-T-H motif By similarity
Experimental info
	Mutagenesis	134	1	E → A in nhaR1; increases the transcription of NhaA.
	Sequence conflict	46 – 47	2	ER → DA Ref.1
	Sequence conflict	46 – 47	2	ER → DA Ref.2
	Sequence conflict	46 – 47	2	ER → DA Ref.3
	Sequence conflict	50	1	G → A Ref.1
	Sequence conflict	50	1	G → A Ref.2
	Sequence conflict	50	1	G → A Ref.3
	Sequence conflict	58 – 65	8	RGLEPSEL → TWSRTQRA Ref.1
	Sequence conflict	58 – 65	8	RGLEPSEL → TWSRTQRA Ref.3
	Sequence conflict	146	1	D → E Ref.1
	Sequence conflict	146	1	D → E Ref.2
	Sequence conflict	146	1	D → E Ref.3
	Sequence conflict	233 – 301	69	FGAMH…SPAVR → WLQVLLVAMQMQSSLPQRFM HMTFMPIKLS Ref.1
	Sequence conflict	233 – 301	69	FGAMH…SPAVR → WLQVLLVAMQMQSSLPQRFM HMTFMPIKLS Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P0A9G2 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: E81FAFC4FC4FD2F1	FASTA	301	34,284
	10 20 30 40 50 60 MSMSHINYNH LYYFWHVYKE GSVVGAAEAL YLTPQTITGQ IRALEERLQG KLFKRKGRGL 70 80 90 100 110 120 EPSELGELVY RYADKMFTLS QEMLDIVNYR KESNLLFDVG VADALSKRLV SSVLNAAVVE 130 140 150 160 170 180 GEPIHLRCFE STHEMLLEQL SQHKLDMIIS DCPIDSTQQE GLFSVRIGEC GVSFWCTNPP 190 200 210 220 230 240 PEKPFPACLE ERRLLIPGRR SMLGRKLLNW FNSQGLNVEI LGEFDDAALM KAFGAMHNAI 250 260 270 280 290 300 FVAPTLYAYD FYADKTVVEI GRVENVMEEY HAIFAERMIQ HPAVQRICNT DYSALFSPAV R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the DNA distal to the gene for ribosomal protein S20 in Escherichia coli K12: presence of a strong terminator and an IS1 element." Mackie G.A. Nucleic Acids Res. 14:6965-6981(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"NhaR, a regulator of nhaA." Harel O. Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"A large family of bacterial activator proteins." Henikoff S., Haughn G.W., Calvo J.M., Wallace J.C. Proc. Natl. Acad. Sci. U.S.A. 85:6602-6606(1988) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROTEIN, POSSIBLE DNA-BINDING REGION.
[7]	"NhaR, a protein homologous to a family of bacterial regulatory proteins (LysR), regulates nhaA, the sodium proton antiporter gene in Escherichia coli." Rahav-Manor O., Carmel O., Karpel R., Taglicht D., Glaser G., Schuldiner S., Padan E. J. Biol. Chem. 267:10433-10438(1992) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"A single amino acid substitution (Glu134-->Ala) in NhaR1 increases the inducibility by Na+ of the product of nhaA, a Na+/H+ antiporter gene in Escherichia coli." Carmel O., Dover N., Rahav-Manor O., Dibrov P., Kirsch D., Karpel R., Schuldiner S., Padan E. EMBO J. 13:1981-1989(1994) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT NHAR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X04382 Genomic DNA. Translation: CAA27969.1. L24072 Genomic DNA. Translation: AAA24221.1. U00096 Genomic DNA. Translation: AAC73131.1. AP009048 Genomic DNA. Translation: BAE76032.1.
RefSeq	NP_414561.1. NC_000913.2. YP_488326.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A9G2.
SMR	P0A9G2. Positions 9-260.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A9G2. 2 interactions.
STRING	511145.b0020.
Proteomic databases
PRIDE	P0A9G2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73131; AAC73131; b0020. BAE76032; BAE76032; BAE76032.
GeneID	12930730. 944757.
KEGG	ecj:Y75_p0020. eco:b0020.
PATRIC	32115131. VBIEscCol129921_0017.
Organism-specific databases
EchoBASE	EB1070.
EcoGene	EG11078. nhaR.
Phylogenomic databases
eggNOG	COG0583.
HOGENOM	HOG000233515.
KO	K03717.
OMA	LCLTPQT.
ProtClustDB	PRK11062.
Enzyme and pathway databases
BioCyc	EcoCyc:PD00474. ECOL316407:JW0019-MONOMER.
Gene expression databases
Genevestigator	P0A9G2.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR005119. LysR_subst-bd. IPR000847. Tscrpt_reg_HTH_LysR. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00126. HTH_1. 1 hit. PF03466. LysR_substrate. 1 hit. [Graphical view]
PROSITE	PS50931. HTH_LYSR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NHAR_ECOLI

Accession

Primary (citable) accession number: P0A9G2
Secondary accession number(s): P10087 , P75619, Q2MCH4, Q47409, Q83SR2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program