P0A9G2 (NHAR_ECOLI) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 1, 2013.
Version 67.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Transcriptional activator protein NhaR Alternative name(s): Na(+)/H(+) antiporter regulatory protein | ||||||
| Gene names |
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| Organism | Escherichia coli (strain K12) [Reference proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › ![]() |
Protein attributes
| Sequence length | 301 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Plays a role in the positive regulation of NhaA. Ref.7 |
| Subcellular location | |
| Sequence similarities | Contains 1 HTH lysR-type DNA-binding domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Transcription Transcription regulation |
| Cellular component | Cytoplasm |
| Ligand | DNA-binding |
| Molecular function | Activator |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | regulation of transcription, DNA-dependent Inferred from expression pattern Ref.7. Source: EcoCyc transcription, DNA-dependentInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular_component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular_function | DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 301 | 301 | Transcriptional activator protein NhaR | PRO_0000105689 | |||||
Regions | |||||||||
| Domain | 6 – 63 | 58 | HTH lysR-type | ||||||
| DNA binding | 23 – 42 | 20 | H-T-H motif By similarity | ||||||
Experimental info | |||||||||
| Mutagenesis | 134 | 1 | E → A in nhaR1; increases the transcription of NhaA. | ||||||
| Sequence conflict | 46 – 47 | 2 | ER → DA Ref.1 | ||||||
| Sequence conflict | 46 – 47 | 2 | ER → DA Ref.2 | ||||||
| Sequence conflict | 46 – 47 | 2 | ER → DA Ref.3 | ||||||
| Sequence conflict | 50 | 1 | G → A Ref.1 | ||||||
| Sequence conflict | 50 | 1 | G → A Ref.2 | ||||||
| Sequence conflict | 50 | 1 | G → A Ref.3 | ||||||
| Sequence conflict | 58 – 65 | 8 | RGLEPSEL → TWSRTQRA Ref.1 | ||||||
| Sequence conflict | 58 – 65 | 8 | RGLEPSEL → TWSRTQRA Ref.3 | ||||||
| Sequence conflict | 146 | 1 | D → E Ref.1 | ||||||
| Sequence conflict | 146 | 1 | D → E Ref.2 | ||||||
| Sequence conflict | 146 | 1 | D → E Ref.3 | ||||||
| Sequence conflict | 233 – 301 | 69 | FGAMH…SPAVR → WLQVLLVAMQMQSSLPQRFM HMTFMPIKLS Ref.1 | ||||||
| Sequence conflict | 233 – 301 | 69 | FGAMH…SPAVR → WLQVLLVAMQMQSSLPQRFM HMTFMPIKLS Ref.3 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Structure of the DNA distal to the gene for ribosomal protein S20 in Escherichia coli K12: presence of a strong terminator and an IS1 element." Mackie G.A. Nucleic Acids Res. 14:6965-6981(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [2] | "NhaR, a regulator of nhaA." Harel O. Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12. |
| [4] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [5] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [6] | "A large family of bacterial activator proteins." Henikoff S., Haughn G.W., Calvo J.M., Wallace J.C. Proc. Natl. Acad. Sci. U.S.A. 85:6602-6606(1988) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROTEIN, POSSIBLE DNA-BINDING REGION. |
| [7] | "NhaR, a protein homologous to a family of bacterial regulatory proteins (LysR), regulates nhaA, the sodium proton antiporter gene in Escherichia coli." Rahav-Manor O., Carmel O., Karpel R., Taglicht D., Glaser G., Schuldiner S., Padan E. J. Biol. Chem. 267:10433-10438(1992) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. |
| [8] | "A single amino acid substitution (Glu134-->Ala) in NhaR1 increases the inducibility by Na+ of the product of nhaA, a Na+/H+ antiporter gene in Escherichia coli." Carmel O., Dover N., Rahav-Manor O., Dibrov P., Kirsch D., Karpel R., Schuldiner S., Padan E. EMBO J. 13:1981-1989(1994) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT NHAR1. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X04382 Genomic DNA. Translation: CAA27969.1. L24072 Genomic DNA. Translation: AAA24221.1. U00096 Genomic DNA. Translation: AAC73131.1. AP009048 Genomic DNA. Translation: BAE76032.1. |
| RefSeq | NP_414561.1. NC_000913.2. YP_488326.1. NC_007779.1. |
3D structure databases | |
| ProteinModelPortal | P0A9G2. |
| SMR | P0A9G2. Positions 9-260. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P0A9G2. 2 interactions. |
| STRING | 511145.b0020. |
Proteomic databases | |
| PRIDE | P0A9G2. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | AAC73131; AAC73131; b0020. BAE76032; BAE76032; BAE76032. |
| GeneID | 12930730. 944757. |
| KEGG | ecj:Y75_p0020. eco:b0020. |
| PATRIC | 32115131. VBIEscCol129921_0017. |
Organism-specific databases | |
| EchoBASE | EB1070. |
| EcoGene | EG11078. nhaR. |
Phylogenomic databases | |
| eggNOG | COG0583. |
| HOGENOM | HOG000233515. |
| KO | K03717. |
| OMA | LCLTPQT. |
| ProtClustDB | PRK11062. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:PD00474. ECOL316407:JW0019-MONOMER. |
Gene expression databases | |
| Genevestigator | P0A9G2. |
Family and domain databases | |
| Gene3D | 1.10.10.10. 1 hit. |
| InterPro | IPR005119. LysR_subst-bd. IPR000847. Tscrpt_reg_HTH_LysR. IPR011991. WHTH_DNA-bd_dom. [Graphical view] |
| Pfam | PF00126. HTH_1. 1 hit. PF03466. LysR_substrate. 1 hit. [Graphical view] |
| PROSITE | PS50931. HTH_LYSR. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | NHAR_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P0A9G2 Secondary accession number(s): P10087 Q83SR2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

Clusters with
