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Protein

Transcriptional activator protein NhaR

Gene

nhaR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the positive regulation of NhaA.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi23 – 4220H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00474.
ECOL316407:JW0019-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional activator protein NhaR
Alternative name(s):
Na(+)/H(+) antiporter regulatory protein
Gene namesi
Name:nhaR
Synonyms:antO
Ordered Locus Names:b0020, JW0019
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11078. nhaR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi134 – 1341E → A in nhaR1; increases the transcription of NhaA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Transcriptional activator protein NhaRPRO_0000105689Add
BLAST

Proteomic databases

PaxDbiP0A9G2.
PRIDEiP0A9G2.

Interactioni

Protein-protein interaction databases

BioGridi4259724. 3 interactions.
IntActiP0A9G2. 2 interactions.
STRINGi511145.b0020.

Structurei

3D structure databases

ProteinModelPortaliP0A9G2.
SMRiP0A9G2. Positions 9-260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 6358HTH lysR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH lysR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41063Z6. Bacteria.
COG0583. LUCA.
HOGENOMiHOG000233515.
InParanoidiP0A9G2.
KOiK03717.
OMAiYYFWHVC.
OrthoDBiEOG6X3W30.
PhylomeDBiP0A9G2.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9G2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMSHINYNH LYYFWHVYKE GSVVGAAEAL YLTPQTITGQ IRALEERLQG
60 70 80 90 100
KLFKRKGRGL EPSELGELVY RYADKMFTLS QEMLDIVNYR KESNLLFDVG
110 120 130 140 150
VADALSKRLV SSVLNAAVVE GEPIHLRCFE STHEMLLEQL SQHKLDMIIS
160 170 180 190 200
DCPIDSTQQE GLFSVRIGEC GVSFWCTNPP PEKPFPACLE ERRLLIPGRR
210 220 230 240 250
SMLGRKLLNW FNSQGLNVEI LGEFDDAALM KAFGAMHNAI FVAPTLYAYD
260 270 280 290 300
FYADKTVVEI GRVENVMEEY HAIFAERMIQ HPAVQRICNT DYSALFSPAV

R
Length:301
Mass (Da):34,284
Last modified:July 19, 2005 - v1
Checksum:iE81FAFC4FC4FD2F1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti46 – 472ER → DA (PubMed:2429258).Curated
Sequence conflicti46 – 472ER → DA (Ref. 2) Curated
Sequence conflicti46 – 472ER → DA (PubMed:1630901).Curated
Sequence conflicti50 – 501G → A (PubMed:2429258).Curated
Sequence conflicti50 – 501G → A (Ref. 2) Curated
Sequence conflicti50 – 501G → A (PubMed:1630901).Curated
Sequence conflicti58 – 658RGLEPSEL → TWSRTQRA (PubMed:2429258).Curated
Sequence conflicti58 – 658RGLEPSEL → TWSRTQRA (PubMed:1630901).Curated
Sequence conflicti146 – 1461D → E (PubMed:2429258).Curated
Sequence conflicti146 – 1461D → E (Ref. 2) Curated
Sequence conflicti146 – 1461D → E (PubMed:1630901).Curated
Sequence conflicti233 – 30169FGAMH…SPAVR → WLQVLLVAMQMQSSLPQRFM HMTFMPIKLS (PubMed:2429258).CuratedAdd
BLAST
Sequence conflicti233 – 30169FGAMH…SPAVR → WLQVLLVAMQMQSSLPQRFM HMTFMPIKLS (PubMed:1630901).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04382 Genomic DNA. Translation: CAA27969.1.
L24072 Genomic DNA. Translation: AAA24221.1.
U00096 Genomic DNA. Translation: AAC73131.1.
AP009048 Genomic DNA. Translation: BAE76032.1.
RefSeqiNP_414561.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC73131; AAC73131; b0020.
BAE76032; BAE76032; BAE76032.
GeneIDi944757.
KEGGiecj:JW0019.
eco:b0020.
PATRICi32115131. VBIEscCol129921_0017.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04382 Genomic DNA. Translation: CAA27969.1.
L24072 Genomic DNA. Translation: AAA24221.1.
U00096 Genomic DNA. Translation: AAC73131.1.
AP009048 Genomic DNA. Translation: BAE76032.1.
RefSeqiNP_414561.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP0A9G2.
SMRiP0A9G2. Positions 9-260.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259724. 3 interactions.
IntActiP0A9G2. 2 interactions.
STRINGi511145.b0020.

Proteomic databases

PaxDbiP0A9G2.
PRIDEiP0A9G2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73131; AAC73131; b0020.
BAE76032; BAE76032; BAE76032.
GeneIDi944757.
KEGGiecj:JW0019.
eco:b0020.
PATRICi32115131. VBIEscCol129921_0017.

Organism-specific databases

EchoBASEiEB1070.
EcoGeneiEG11078. nhaR.

Phylogenomic databases

eggNOGiENOG41063Z6. Bacteria.
COG0583. LUCA.
HOGENOMiHOG000233515.
InParanoidiP0A9G2.
KOiK03717.
OMAiYYFWHVC.
OrthoDBiEOG6X3W30.
PhylomeDBiP0A9G2.

Enzyme and pathway databases

BioCyciEcoCyc:PD00474.
ECOL316407:JW0019-MONOMER.

Miscellaneous databases

PROiP0A9G2.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of the DNA distal to the gene for ribosomal protein S20 in Escherichia coli K12: presence of a strong terminator and an IS1 element."
    Mackie G.A.
    Nucleic Acids Res. 14:6965-6981(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "NhaR, a regulator of nhaA."
    Harel O.
    Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region."
    Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A.
    Nucleic Acids Res. 20:3305-3308(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. Cited for: IDENTIFICATION OF PROTEIN, POSSIBLE DNA-BINDING REGION.
  7. "NhaR, a protein homologous to a family of bacterial regulatory proteins (LysR), regulates nhaA, the sodium proton antiporter gene in Escherichia coli."
    Rahav-Manor O., Carmel O., Karpel R., Taglicht D., Glaser G., Schuldiner S., Padan E.
    J. Biol. Chem. 267:10433-10438(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "A single amino acid substitution (Glu134-->Ala) in NhaR1 increases the inducibility by Na+ of the product of nhaA, a Na+/H+ antiporter gene in Escherichia coli."
    Carmel O., Dover N., Rahav-Manor O., Dibrov P., Kirsch D., Karpel R., Schuldiner S., Padan E.
    EMBO J. 13:1981-1989(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANT NHAR1.

Entry informationi

Entry nameiNHAR_ECOLI
AccessioniPrimary (citable) accession number: P0A9G2
Secondary accession number(s): P10087
, P75619, Q2MCH4, Q47409, Q83SR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: January 20, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.