HTH-type transcriptional regulator MetR - Escherichia coli (strain K12)

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P0A9F9 (METR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 68. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
HTH-type transcriptional regulator MetR

Gene names

Name:	metR
Ordered Locus Names:	b3828, JW3804

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Control of the last step in methionine biosynthesis; MetR is a positive activator of the metA, metE and metH genes. MetR is also a negative regulator of its own expression. Binds homocysteine as an inducer.
Subcellular location	Cytoplasm.
Sequence similarities	Contains 1 HTH lysR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from mutant phenotype PubMed 9767577. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from direct assay PubMed 2543976. Source: EcoCyc amino acid binding Inferred from direct assay PubMed 2543976. Source: EcoCyc sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 317

317

HTH-type transcriptional regulator MetR

PRO_0000105673

Regions

Domain

1 – 59

HTH lysR-type

DNA binding

19 – 38

H-T-H motif By similarity

Experimental info

Sequence conflict

L → T Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P0A9F9 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: 513F5C710803800D
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FASTA

317

35,629

        10         20         30         40         50         60 
MIEVKHLKTL QALRNCGSLA AAAATLHQTQ SALSHQFSDL EQRLGFRLFV RKSQPLRFTP 

        70         80         90        100        110        120 
QGEILLQLAN QVLPQISQAL QACNEPQQTR LRIAIECHSC IQWLTPALEN FHKNWPQVEM 

       130        140        150        160        170        180 
DFKSGVTFDP QPALQQGELD LVMTSDILPR SGLHYSPMFD YEVRLVLAPD HPLAAKTRIT 

       190        200        210        220        230        240 
PEDLASETLL IYPVQRSRLD VWRHFLQPAG VSPSLKSVDN TLLLIQMVAA RMGIAALPHW 

       250        260        270        280        290        300 
VVESFERQGL VVTKTLGEGL WSRLYAAVRD GEQRQPVTEA FIRSARNHAC DHLPFVKSAE 

       310 
RPTYDAPTVR PGSPARL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure-function studies on Escherichia coli MetR protein, a putative prokaryotic leucine zipper protein." Maxon M.E., Wigboldus J., Brot N., Weissbach H. Proc. Natl. Acad. Sci. U.S.A. 87:7076-7079(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R. Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Regulation of methionine synthesis in Escherichia coli: effect of the MetR protein on the expression of the metE and metR genes." Maxon M.E., Redfield B., Cai X.-Y., Shoeman R., Fujita K., Fisher W., Stauffer G., Weissbach H., Brot N. Proc. Natl. Acad. Sci. U.S.A. 86:85-89(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M37630 Genomic DNA. Translation: AAA62781.1. M87049 Genomic DNA. Translation: AAA67624.1. U00096 Genomic DNA. Translation: AAC76831.1. AP009048 Genomic DNA. Translation: BAE77473.1. J04155 Genomic DNA. Translation: AAA24159.2. Sequence problems.
PIR	A36066.
RefSeq	NP_418272.1. NC_000913.2. YP_491614.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A9F9.
SMR	P0A9F9. Positions 6-300.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A9F9. 6 interactions.
MINT	MINT-1262668.
STRING	511145.b3828.
Proteomic databases
PaxDb	P0A9F9.
PRIDE	P0A9F9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76831; AAC76831; b3828. BAE77473; BAE77473; BAE77473.
GeneID	12930482. 948310.
KEGG	ecj:Y75_p3350. eco:b3828.
PATRIC	32123157. VBIEscCol129921_3944.
Organism-specific databases
EchoBASE	EB0586.
EcoGene	EG10591. metR.
Phylogenomic databases
eggNOG	COG0583.
HOGENOM	HOG000218906.
KO	K03576.
OMA	PHQTTLR.
ProtClustDB	PRK15421.
Enzyme and pathway databases
BioCyc	EcoCyc:PD03938. ECOL316407:JW3804-MONOMER.
Gene expression databases
Genevestigator	P0A9F9.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR005119. LysR_subst-bd. IPR000847. Tscrpt_reg_HTH_LysR. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00126. HTH_1. 1 hit. PF03466. LysR_substrate. 1 hit. [Graphical view]
PRINTS	PR00039. HTHLYSR.
PROSITE	PS50931. HTH_LYSR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METR_ECOLI

Accession

Primary (citable) accession number: P0A9F9
Secondary accession number(s): P19797, Q2M8D3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents