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Protein

HTH-type transcriptional regulator MetR

Gene

metR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Control of the last step in methionine biosynthesis; MetR is a positive activator of the metA, metE and metH genes. MetR is also a negative regulator of its own expression. Binds homocysteine as an inducer.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi19 – 3820H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • amino acid binding Source: EcoCyc
  • DNA binding Source: EcoCyc
  • transcription factor activity, sequence-specific DNA binding Source: InterPro

GO - Biological processi

  • methionine biosynthetic process Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD03938.
ECOL316407:JW3804-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator MetR
Gene namesi
Name:metR
Ordered Locus Names:b3828, JW3804
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10591. metR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317HTH-type transcriptional regulator MetRPRO_0000105673Add
BLAST

Proteomic databases

PaxDbiP0A9F9.
PRIDEiP0A9F9.

Interactioni

Protein-protein interaction databases

BioGridi4259476. 2 interactions.
DIPiDIP-10198N.
IntActiP0A9F9. 8 interactions.
MINTiMINT-1262668.
STRINGi511145.b3828.

Structurei

3D structure databases

ProteinModelPortaliP0A9F9.
SMRiP0A9F9. Positions 6-300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5959HTH lysR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH lysR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105DZ2. Bacteria.
ENOG410XQG7. LUCA.
HOGENOMiHOG000218906.
InParanoidiP0A9F9.
KOiK03576.
OMAiACEHLPF.
OrthoDBiEOG64BQ3T.
PhylomeDBiP0A9F9.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9F9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEVKHLKTL QALRNCGSLA AAAATLHQTQ SALSHQFSDL EQRLGFRLFV
60 70 80 90 100
RKSQPLRFTP QGEILLQLAN QVLPQISQAL QACNEPQQTR LRIAIECHSC
110 120 130 140 150
IQWLTPALEN FHKNWPQVEM DFKSGVTFDP QPALQQGELD LVMTSDILPR
160 170 180 190 200
SGLHYSPMFD YEVRLVLAPD HPLAAKTRIT PEDLASETLL IYPVQRSRLD
210 220 230 240 250
VWRHFLQPAG VSPSLKSVDN TLLLIQMVAA RMGIAALPHW VVESFERQGL
260 270 280 290 300
VVTKTLGEGL WSRLYAAVRD GEQRQPVTEA FIRSARNHAC DHLPFVKSAE
310
RPTYDAPTVR PGSPARL
Length:317
Mass (Da):35,629
Last modified:July 19, 2005 - v1
Checksum:i513F5C710803800D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti19 – 191L → T (PubMed:2643109).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37630 Genomic DNA. Translation: AAA62781.1.
M87049 Genomic DNA. Translation: AAA67624.1.
U00096 Genomic DNA. Translation: AAC76831.1.
AP009048 Genomic DNA. Translation: BAE77473.1.
J04155 Genomic DNA. Translation: AAA24159.2. Sequence problems.
PIRiA36066.
RefSeqiNP_418272.1. NC_000913.3.
WP_000573621.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76831; AAC76831; b3828.
BAE77473; BAE77473; BAE77473.
GeneIDi948310.
KEGGiecj:JW3804.
eco:b3828.
PATRICi32123157. VBIEscCol129921_3944.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M37630 Genomic DNA. Translation: AAA62781.1.
M87049 Genomic DNA. Translation: AAA67624.1.
U00096 Genomic DNA. Translation: AAC76831.1.
AP009048 Genomic DNA. Translation: BAE77473.1.
J04155 Genomic DNA. Translation: AAA24159.2. Sequence problems.
PIRiA36066.
RefSeqiNP_418272.1. NC_000913.3.
WP_000573621.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A9F9.
SMRiP0A9F9. Positions 6-300.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259476. 2 interactions.
DIPiDIP-10198N.
IntActiP0A9F9. 8 interactions.
MINTiMINT-1262668.
STRINGi511145.b3828.

Proteomic databases

PaxDbiP0A9F9.
PRIDEiP0A9F9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76831; AAC76831; b3828.
BAE77473; BAE77473; BAE77473.
GeneIDi948310.
KEGGiecj:JW3804.
eco:b3828.
PATRICi32123157. VBIEscCol129921_3944.

Organism-specific databases

EchoBASEiEB0586.
EcoGeneiEG10591. metR.

Phylogenomic databases

eggNOGiENOG4105DZ2. Bacteria.
ENOG410XQG7. LUCA.
HOGENOMiHOG000218906.
InParanoidiP0A9F9.
KOiK03576.
OMAiACEHLPF.
OrthoDBiEOG64BQ3T.
PhylomeDBiP0A9F9.

Enzyme and pathway databases

BioCyciEcoCyc:PD03938.
ECOL316407:JW3804-MONOMER.

Miscellaneous databases

PROiP0A9F9.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure-function studies on Escherichia coli MetR protein, a putative prokaryotic leucine zipper protein."
    Maxon M.E., Wigboldus J., Brot N., Weissbach H.
    Proc. Natl. Acad. Sci. U.S.A. 87:7076-7079(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Regulation of methionine synthesis in Escherichia coli: effect of the MetR protein on the expression of the metE and metR genes."
    Maxon M.E., Redfield B., Cai X.-Y., Shoeman R., Fujita K., Fisher W., Stauffer G., Weissbach H., Brot N.
    Proc. Natl. Acad. Sci. U.S.A. 86:85-89(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19.

Entry informationi

Entry nameiMETR_ECOLI
AccessioniPrimary (citable) accession number: P0A9F9
Secondary accession number(s): P19797, Q2M8D3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: January 20, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.