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Protein

Glycine cleavage system transcriptional activator

Gene

gcvA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Binds to three sites upstream of the gcv promoter.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi23 – 4220H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription, DNA-templated Source: EcoliWiki
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00339.
ECOL316407:JW2779-MONOMER.
RETL1328306-WGS:GSTH-3175-MONOMER.
RETL1328306-WGS:GSTH-4145-MONOMER.
RETL1328306-WGS:GSTH-4249-MONOMER.
RETL1328306-WGS:GSTH-4578-MONOMER.
RETL1328306-WGS:GSTH-5457-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine cleavage system transcriptional activator
Alternative name(s):
Gcv operon activator
Gene namesi
Name:gcvA
Ordered Locus Names:b2808, JW2779
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11795. gcvA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 305305Glycine cleavage system transcriptional activatorPRO_0000105625Add
BLAST

Proteomic databases

PaxDbiP0A9F6.
PRIDEiP0A9F6.

Interactioni

Protein-protein interaction databases

BioGridi4261124. 25 interactions.
DIPiDIP-9751N.
IntActiP0A9F6. 2 interactions.
STRINGi511145.b2808.

Structurei

3D structure databases

ProteinModelPortaliP0A9F6.
SMRiP0A9F6. Positions 8-288.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 6358HTH lysR-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH lysR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105ETT. Bacteria.
ENOG410XS6G. LUCA.
HOGENOMiHOG000233528.
InParanoidiP0A9F6.
KOiK03566.
OMAiDMQQQIN.
PhylomeDBiP0A9F6.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9F6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKRLPPLNA LRVFDAAARH LSFTRAAEEL FVTQAAVSHQ IKSLEDFLGL
60 70 80 90 100
KLFRRRNRSL LLTEEGQSYF LDIKEIFSQL TEATRKLQAR SAKGALTVSL
110 120 130 140 150
LPSFAIHWLV PRLSSFNSAY PGIDVRIQAV DRQEDKLADD VDVAIFYGRG
160 170 180 190 200
NWPGLRVEKL YAEYLLPVCS PLLLTGEKPL KTPEDLAKHT LLHDASRRDW
210 220 230 240 250
QTYTRQLGLN HINVQQGPIF SHSAMVLQAA IHGQGVALAN NVMAQSEIEA
260 270 280 290 300
GRLVCPFNDV LVSKNAFYLV CHDSQAELGK IAAFRQWILA KAAAEQEKFR

FRYEQ
Length:305
Mass (Da):34,402
Last modified:July 19, 2005 - v1
Checksum:i42AE40C64DAB5F72
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01030 Genomic DNA. Translation: AAC13742.1.
X73413 Genomic DNA. Translation: CAA51813.1.
U29581 Genomic DNA. Translation: AAB40458.1.
U00096 Genomic DNA. Translation: AAC75850.1.
AP009048 Genomic DNA. Translation: BAE76880.1.
PIRiI41065.
RefSeqiNP_417288.1. NC_000913.3.
WP_000044401.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75850; AAC75850; b2808.
BAE76880; BAE76880; BAE76880.
GeneIDi947278.
KEGGiecj:JW2779.
eco:b2808.
PATRICi32121032. VBIEscCol129921_2908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01030 Genomic DNA. Translation: AAC13742.1.
X73413 Genomic DNA. Translation: CAA51813.1.
U29581 Genomic DNA. Translation: AAB40458.1.
U00096 Genomic DNA. Translation: AAC75850.1.
AP009048 Genomic DNA. Translation: BAE76880.1.
PIRiI41065.
RefSeqiNP_417288.1. NC_000913.3.
WP_000044401.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A9F6.
SMRiP0A9F6. Positions 8-288.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261124. 25 interactions.
DIPiDIP-9751N.
IntActiP0A9F6. 2 interactions.
STRINGi511145.b2808.

Proteomic databases

PaxDbiP0A9F6.
PRIDEiP0A9F6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75850; AAC75850; b2808.
BAE76880; BAE76880; BAE76880.
GeneIDi947278.
KEGGiecj:JW2779.
eco:b2808.
PATRICi32121032. VBIEscCol129921_2908.

Organism-specific databases

EchoBASEiEB1743.
EcoGeneiEG11795. gcvA.

Phylogenomic databases

eggNOGiENOG4105ETT. Bacteria.
ENOG410XS6G. LUCA.
HOGENOMiHOG000233528.
InParanoidiP0A9F6.
KOiK03566.
OMAiDMQQQIN.
PhylomeDBiP0A9F6.

Enzyme and pathway databases

BioCyciEcoCyc:PD00339.
ECOL316407:JW2779-MONOMER.
RETL1328306-WGS:GSTH-3175-MONOMER.
RETL1328306-WGS:GSTH-4145-MONOMER.
RETL1328306-WGS:GSTH-4249-MONOMER.
RETL1328306-WGS:GSTH-4578-MONOMER.
RETL1328306-WGS:GSTH-5457-MONOMER.

Miscellaneous databases

PROiP0A9F6.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGCVA_ECOLI
AccessioniPrimary (citable) accession number: P0A9F6
Secondary accession number(s): P32064, Q2MA26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.