Reviewed, UniProtKB/Swiss-Prot P0A9F6 (GCVA_ECOLI)

Names and origin

Protein names

Recommended name:
    Glycine cleavage system transcriptional activator
Alternative name(s):
    Gcv operon activator

Gene names

Name:	gcvA
Ordered Locus Names:	b2808, JW2779

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter.
Subcellular location	Cytoplasm.
Sequence similarities	Contains 1 HTH lysR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator Repressor
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
groL	P0A6F5	1	EBI-1130436,EBI-543750

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Glycine cleavage system transcriptional activator		PRO_0000105625
Regions
	Domain	6 – 63	58	HTH lysR-type
	DNA binding	23 – 42	20	H-T-H motif By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A9F6-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 42AE40C64DAB5F72	FASTA	305	34,402
	10 20 30 40 50 60 MSKRLPPLNA LRVFDAAARH LSFTRAAEEL FVTQAAVSHQ IKSLEDFLGL KLFRRRNRSL 70 80 90 100 110 120 LLTEEGQSYF LDIKEIFSQL TEATRKLQAR SAKGALTVSL LPSFAIHWLV PRLSSFNSAY 130 140 150 160 170 180 PGIDVRIQAV DRQEDKLADD VDVAIFYGRG NWPGLRVEKL YAEYLLPVCS PLLLTGEKPL 190 200 210 220 230 240 KTPEDLAKHT LLHDASRRDW QTYTRQLGLN HINVQQGPIF SHSAMVLQAA IHGQGVALAN 250 260 270 280 290 300 NVMAQSEIEA GRLVCPFNDV LVSKNAFYLV CHDSQAELGK IAAFRQWILA KAAAEQEKFR FRYEQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"DNA sequence and characterization of GcvA, a LysR family regulatory protein for the Escherichia coli glycine cleavage enzyme system." Wilson R.L., Stauffer G.V. J. Bacteriol. 176:2862-2868(1994) [PubMed: 8188587] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: K12.
[2]	"GcvA, a LysR-type transcriptional regulator protein, activates expression of the cloned Citrobacter freundii ampC beta-lactamase gene in Escherichia coli: cross-talk between DNA-binding proteins." Everett M.J., Walsh T., Guay G., Bennett P.M. Microbiology 141:419-430(1995) [PubMed: 7704273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U01030 Genomic DNA. Translation: AAC13742.1. X73413 Genomic DNA. Translation: CAA51813.1. U29581 Genomic DNA. Translation: AAB40458.1. U00096 Genomic DNA. Translation: AAC75850.1. AP009048 Genomic DNA. Translation: BAE76880.1.
PIR	I41065.
RefSeq	AP_003374.1. NP_417288.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A9F6. 1 interaction.
Genome annotation databases
GeneID	947278.
GenomeReviews	Gene locus JW2779 in contig AP009048_GR. Gene locus b2808 in contig U00096_GR.
KEGG	ecj:JW2779. eco:b2808.
Organism-specific databases
EchoBASE	EB1743.
EcoGene	EG11795. gcvA.
CMR	Search...
Phylogenomic databases
HOGENOM	P0A9F6.
OMA	P0A9F6. EEYLGLK.
Enzyme and pathway databases
BioCyc	EcoCyc:PD00339.
Family and domain databases
InterPro	IPR000847. HTH_LysR. IPR005119. LysR_subst_bd. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF00126. HTH_1. 1 hit. PF03466. LysR_substrate. 1 hit. [Graphical view]
PRINTS	PR00039. HTHLYSR.
PROSITE	PS50931. HTH_LYSR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCVA_ECOLI

Accession

Primary (citable) accession number: P0A9F6
Secondary accession number(s): P32064, Q2MA26

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)