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Protein

HTH-type transcriptional regulator CysB

Gene

cysB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This protein is a positive regulator of gene expression for the cysteine regulon, a system of 10 or more loci involved in the biosynthesis of L-cysteine from inorganic sulfate. The inducer for CysB is N-acetylserine. CysB inhibits its own transcription.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi19 – 38H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • DNA binding Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • transcription factor activity, sequence-specific DNA binding Source: InterPro

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00232.
ECOL316407:JW1267-MONOMER.
MetaCyc:PD00232.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator CysB
Alternative name(s):
Cys regulon transcriptional activator
Gene namesi
Name:cysB
Ordered Locus Names:b1275, JW1267
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10184. cysB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001056131 – 324HTH-type transcriptional regulator CysBAdd BLAST324

Proteomic databases

EPDiP0A9F3.
PaxDbiP0A9F3.
PRIDEiP0A9F3.

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4260138. 21 interactors.
DIPiDIP-36048N.
IntActiP0A9F3. 9 interactors.
MINTiMINT-1276319.
STRINGi511145.b1275.

Structurei

3D structure databases

ProteinModelPortaliP0A9F3.
SMRiP0A9F3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 59HTH lysR-typePROSITE-ProRule annotationAdd BLAST59

Sequence similaritiesi

Contains 1 HTH lysR-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4107R11. Bacteria.
ENOG410XP61. LUCA.
HOGENOMiHOG000260068.
InParanoidiP0A9F3.
KOiK13634.
OMAiDEAFSHR.
PhylomeDBiP0A9F3.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9F3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLQQLRYIV EVVNHNLNVS STAEGLYTSQ PGISKQVRML EDELGIQIFS
60 70 80 90 100
RSGKHLTQVT PAGQEIIRIA REVLSKVDAI KSVAGEHTWP DKGSLYIATT
110 120 130 140 150
HTQARYALPN VIKGFIERYP RVSLHMHQGS PTQIADAVSK GNADFAIATE
160 170 180 190 200
ALHLYEDLVM LPCYHWNRAI VVTPDHPLAG KKAITIEELA QYPLVTYTFG
210 220 230 240 250
FTGRSELDTA FNRAGLTPRI VFTATDADVI KTYVRLGLGV GVIASMAVDP
260 270 280 290 300
VADPDLVRVD AHDIFSHSTT KIGFRRSTFL RSYMYDFIQR FAPHLTRDVV
310 320
DAAVALRSNE EIEVMFKDIK LPEK
Length:324
Mass (Da):36,150
Last modified:January 1, 1988 - v1
Checksum:i3B35E9CFEF6F4C84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15041 Genomic DNA. Translation: AAA23642.1.
M34332 Genomic DNA. Translation: AAA23643.1.
U00096 Genomic DNA. Translation: AAC74357.1.
AP009048 Genomic DNA. Translation: BAA14827.1.
X60293 Genomic DNA. Translation: CAA42833.1.
PIRiA26695. RGECCB.
RefSeqiNP_415791.1. NC_000913.3.
WP_000776253.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74357; AAC74357; b1275.
BAA14827; BAA14827; BAA14827.
GeneIDi945771.
KEGGiecj:JW1267.
eco:b1275.
PATRICi32117808. VBIEscCol129921_1324.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15041 Genomic DNA. Translation: AAA23642.1.
M34332 Genomic DNA. Translation: AAA23643.1.
U00096 Genomic DNA. Translation: AAC74357.1.
AP009048 Genomic DNA. Translation: BAA14827.1.
X60293 Genomic DNA. Translation: CAA42833.1.
PIRiA26695. RGECCB.
RefSeqiNP_415791.1. NC_000913.3.
WP_000776253.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A9F3.
SMRiP0A9F3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260138. 21 interactors.
DIPiDIP-36048N.
IntActiP0A9F3. 9 interactors.
MINTiMINT-1276319.
STRINGi511145.b1275.

Proteomic databases

EPDiP0A9F3.
PaxDbiP0A9F3.
PRIDEiP0A9F3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74357; AAC74357; b1275.
BAA14827; BAA14827; BAA14827.
GeneIDi945771.
KEGGiecj:JW1267.
eco:b1275.
PATRICi32117808. VBIEscCol129921_1324.

Organism-specific databases

EchoBASEiEB0181.
EcoGeneiEG10184. cysB.

Phylogenomic databases

eggNOGiENOG4107R11. Bacteria.
ENOG410XP61. LUCA.
HOGENOMiHOG000260068.
InParanoidiP0A9F3.
KOiK13634.
OMAiDEAFSHR.
PhylomeDBiP0A9F3.

Enzyme and pathway databases

BioCyciEcoCyc:PD00232.
ECOL316407:JW1267-MONOMER.
MetaCyc:PD00232.

Miscellaneous databases

PROiP0A9F3.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSiPR00039. HTHLYSR.
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS50931. HTH_LYSR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSB_ECOLI
AccessioniPrimary (citable) accession number: P0A9F3
Secondary accession number(s): P06613, P76834
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: November 2, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.