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P0A9F3 (CYSB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
HTH-type transcriptional regulator CysB
Alternative name(s):
Cys regulon transcriptional activator
Gene names
Name:cysB
Ordered Locus Names:b1275, JW1267
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This protein is a positive regulator of gene expression for the cysteine regulon, a system of 10 or more loci involved in the biosynthesis of L-cysteine from inorganic sulfate. The inducer for CysB is N-acetylserine. CysB inhibits its own transcription.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Contains 1 HTH lysR-type DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324HTH-type transcriptional regulator CysB
PRO_0000105613

Regions

Domain1 – 5959HTH lysR-type
DNA binding19 – 3820H-T-H motif By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A9F3 [UniParc].

Last modified January 1, 1988. Version 1.
Checksum: 3B35E9CFEF6F4C84

FASTA32436,150
        10         20         30         40         50         60 
MKLQQLRYIV EVVNHNLNVS STAEGLYTSQ PGISKQVRML EDELGIQIFS RSGKHLTQVT 

        70         80         90        100        110        120 
PAGQEIIRIA REVLSKVDAI KSVAGEHTWP DKGSLYIATT HTQARYALPN VIKGFIERYP 

       130        140        150        160        170        180 
RVSLHMHQGS PTQIADAVSK GNADFAIATE ALHLYEDLVM LPCYHWNRAI VVTPDHPLAG 

       190        200        210        220        230        240 
KKAITIEELA QYPLVTYTFG FTGRSELDTA FNRAGLTPRI VFTATDADVI KTYVRLGLGV 

       250        260        270        280        290        300 
GVIASMAVDP VADPDLVRVD AHDIFSHSTT KIGFRRSTFL RSYMYDFIQR FAPHLTRDVV 

       310        320 
DAAVALRSNE EIEVMFKDIK LPEK

« Hide

References

« Hide 'large scale' references
[1]"DNA sequences of the cysB regions of Salmonella typhimurium and Escherichia coli."
Ostrowski J., Jagura-Burdzy G., Kredich N.M.
J. Biol. Chem. 262:5999-6005(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of the Escherichia coli K-12 cysB gene and its product using the method of gene fusion."
Tei H., Watanabe K., Murata K., Kimura A.
Biochem. Biophys. Res. Commun. 167:962-969(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"The aconitase of Escherichia coli. Nucleotide sequence of the aconitase gene and amino acid sequence similarity with mitochondrial aconitases, the iron-responsive-element-binding protein and isopropylmalate isomerases."
Prodromou C., Artymiuk P.J., Guest J.R.
Eur. J. Biochem. 204:599-609(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 249-324.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M15041 Genomic DNA. Translation: AAA23642.1.
M34332 Genomic DNA. Translation: AAA23643.1.
U00096 Genomic DNA. Translation: AAC74357.1.
AP009048 Genomic DNA. Translation: BAA14827.1.
X60293 Genomic DNA. Translation: CAA42833.1.
PIRRGECCB. A26695.
RefSeqNP_415791.1. NC_000913.2.
YP_489543.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0A9F3.
SMRP0A9F3. Positions 1-324.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-36048N.
IntActP0A9F3. 7 interactions.
MINTMINT-1276319.
STRING511145.b1275.

Proteomic databases

PaxDbP0A9F3.
PRIDEP0A9F3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74357; AAC74357; b1275.
BAA14827; BAA14827; BAA14827.
GeneID12931144.
945771.
KEGGecj:Y75_p1249.
eco:b1275.
PATRIC32117808. VBIEscCol129921_1324.

Organism-specific databases

EchoBASEEB0181.
EcoGeneEG10184. cysB.

Phylogenomic databases

eggNOGCOG0583.
HOGENOMHOG000260068.
KOK13634.
OMAYMYDFIE.
ProtClustDBPRK12681.

Enzyme and pathway databases

BioCycEcoCyc:PD00232.
ECOL316407:JW1267-MONOMER.
MetaCyc:PD00232.

Gene expression databases

GenevestigatorP0A9F3.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR005119. LysR_subst-bd.
IPR000847. Tscrpt_reg_HTH_LysR.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00126. HTH_1. 1 hit.
PF03466. LysR_substrate. 1 hit.
[Graphical view]
PRINTSPR00039. HTHLYSR.
PROSITEPS50931. HTH_LYSR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSB_ECOLI
AccessionPrimary (citable) accession number: P0A9F3
Secondary accession number(s): P06613, P76834
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: January 1, 1988
Last modified: May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents