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Protein

Regulatory protein YeiL

Gene

yeiL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transcription regulator involved in mid-term, stationary-phase viability under nitrogen starvation. Might control expression of the salvage pathways or in some other way repress the recycling of nucleobases to nucleic acids and enhance their use as general nitrogen sources during nitrogen-limited growth.1 Publication

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi68 – 681Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi91 – 911Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi93 – 931Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi116 – 1161Iron-sulfur (4Fe-4S)Sequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi158 – 18124H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12031-MONOMER.
ECOL316407:JW2150-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein YeiL
Gene namesi
Name:yeiL
Synonyms:nsr
Ordered Locus Names:b2163, JW2150
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG12031. yeiL.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 219219Regulatory protein YeiLPRO_0000100182Add
BLAST

Proteomic databases

PRIDEiP0A9E9.

Expressioni

Inductioni

In the stationary phase, aerobic expression is positively autoregulated and significantly dependent on RpoS and Lrp. Its transcription is directly or indirectly repressed by FNR.1 Publication

Gene expression databases

GenevestigatoriP0A9E9.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi511145.b2163.

Structurei

3D structure databases

ProteinModelPortaliP0A9E9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini136 – 19964HTH crp-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 9779Sensory domainSequence AnalysisAdd
BLAST
Regioni111 – 13121Dimer interfaceSequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 HTH crp-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000094699.
KOiK16326.
OMAiITRTENY.
OrthoDBiEOG686NMR.
PhylomeDBiP0A9E9.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9E9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSESAFKDCF LTDVSADTRL FHFLARDYIV QEGQQPSWLF YLTRGRARLY
60 70 80 90 100
ATLANGRVSL IDFFAAPCFI GEIELIDKDH EPRAVQAIEE CWCLALPMKH
110 120 130 140 150
YRPLLLNDTL FLRKLCVTLS HKNYRNIVSL TQNQSFPLVN RLAAFILLSQ
160 170 180 190 200
EGDLYHEKHT QAAEYLGVSY RHLLYVLAQF IHDGLLIKSK KGYLIKNRKQ
210
LSGLALEMDP ENKFSGMMQ
Length:219
Mass (Da):25,294
Last modified:July 19, 2005 - v1
Checksum:i47720B07A9FD412D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA. Translation: AAA60515.1.
U00096 Genomic DNA. Translation: AAC75224.1.
AP009048 Genomic DNA. Translation: BAE76640.1.
PIRiB64985.
RefSeqiNP_416668.3. NC_000913.3.
YP_490402.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75224; AAC75224; b2163.
BAE76640; BAE76640; BAE76640.
GeneIDi12931468.
946670.
KEGGiecj:Y75_p2125.
eco:b2163.
PATRICi32119675. VBIEscCol129921_2248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA. Translation: AAA60515.1.
U00096 Genomic DNA. Translation: AAC75224.1.
AP009048 Genomic DNA. Translation: BAE76640.1.
PIRiB64985.
RefSeqiNP_416668.3. NC_000913.3.
YP_490402.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP0A9E9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi511145.b2163.

Proteomic databases

PRIDEiP0A9E9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75224; AAC75224; b2163.
BAE76640; BAE76640; BAE76640.
GeneIDi12931468.
946670.
KEGGiecj:Y75_p2125.
eco:b2163.
PATRICi32119675. VBIEscCol129921_2248.

Organism-specific databases

EchoBASEiEB1966.
EcoGeneiEG12031. yeiL.

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000094699.
KOiK16326.
OMAiITRTENY.
OrthoDBiEOG686NMR.
PhylomeDBiP0A9E9.

Enzyme and pathway databases

BioCyciEcoCyc:EG12031-MONOMER.
ECOL316407:JW2150-MONOMER.

Miscellaneous databases

PROiP0A9E9.

Gene expression databases

GenevestigatoriP0A9E9.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Automated multiplex sequencing of the E.coli genome."
    Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
    Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / BHB2600.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "YeiL, the third member of the CRP-FNR family in Escherichia coli."
    Anjum M.F., Green J., Guest J.R.
    Microbiology 146:3157-3170(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiYEIL_ECOLI
AccessioniPrimary (citable) accession number: P0A9E9
Secondary accession number(s): P33023, Q2MAR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: January 7, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Its activity might be modulated by a sensory iron-sulfur cluster, by reversible dithiol-disulfide formation, or by both mechanisms. It is not an oxygen-responsive regulator.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.