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P0A9E9 (YEIL_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulatory protein YeiL
Gene names
Name:yeiL
Synonyms:nsr
Ordered Locus Names:b2163, JW2150
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcription regulator involved in mid-term, stationary-phase viability under nitrogen starvation. Might control expression of the salvage pathways or in some other way repress the recycling of nucleobases to nucleic acids and enhance their use as general nitrogen sources during nitrogen-limited growth. Ref.4

Cofactor

Binds 1 4Fe-4S cluster per subunit Probable. Ref.4

Subunit structure

Homodimer Probable. Ref.4

Subcellular location

Cytoplasm Probable Ref.4.

Induction

In the stationary phase, aerobic expression is positively autoregulated and significantly dependent on RpoS and Lrp. Its transcription is directly or indirectly repressed by FNR. Ref.4

Miscellaneous

Its activity might be modulated by a sensory iron-sulfur cluster, by reversible dithiol-disulfide formation, or by both mechanisms. It is not an oxygen-responsive regulator.

Sequence similarities

Contains 1 cyclic nucleotide-binding domain.

Contains 1 HTH crp-type DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 219219Regulatory protein YeiL
PRO_0000100182

Regions

Domain136 – 19964HTH crp-type
DNA binding158 – 18124H-T-H motif Potential
Region19 – 9779Sensory domain Potential
Region111 – 13121Dimer interface Potential

Sites

Metal binding681Iron-sulfur (4Fe-4S) Potential
Metal binding911Iron-sulfur (4Fe-4S) Potential
Metal binding931Iron-sulfur (4Fe-4S) Potential
Metal binding1161Iron-sulfur (4Fe-4S) Potential

Sequences

Sequence LengthMass (Da)Tools
P0A9E9 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: 47720B07A9FD412D

FASTA21925,294
        10         20         30         40         50         60 
MSESAFKDCF LTDVSADTRL FHFLARDYIV QEGQQPSWLF YLTRGRARLY ATLANGRVSL 

        70         80         90        100        110        120 
IDFFAAPCFI GEIELIDKDH EPRAVQAIEE CWCLALPMKH YRPLLLNDTL FLRKLCVTLS 

       130        140        150        160        170        180 
HKNYRNIVSL TQNQSFPLVN RLAAFILLSQ EGDLYHEKHT QAAEYLGVSY RHLLYVLAQF 

       190        200        210 
IHDGLLIKSK KGYLIKNRKQ LSGLALEMDP ENKFSGMMQ 

« Hide

References

« Hide 'large scale' references
[1]"Automated multiplex sequencing of the E.coli genome."
Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K., Church G.M.
Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / BHB2600.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"YeiL, the third member of the CRP-FNR family in Escherichia coli."
Anjum M.F., Green J., Guest J.R.
Microbiology 146:3157-3170(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00007 Genomic DNA. Translation: AAA60515.1.
U00096 Genomic DNA. Translation: AAC75224.1.
AP009048 Genomic DNA. Translation: BAE76640.1.
PIRB64985.
RefSeqNP_416668.3. NC_000913.3.
YP_490402.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0A9E9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING511145.b2163.

Proteomic databases

PRIDEP0A9E9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75224; AAC75224; b2163.
BAE76640; BAE76640; BAE76640.
GeneID12931468.
946670.
KEGGecj:Y75_p2125.
eco:b2163.
PATRIC32119675. VBIEscCol129921_2248.

Organism-specific databases

EchoBASEEB1966.
EcoGeneEG12031. yeiL.

Phylogenomic databases

eggNOGCOG0664.
HOGENOMHOG000094699.
KOK16326.
OMACFIGEME.
OrthoDBEOG686NMR.
PhylomeDBP0A9E9.

Enzyme and pathway databases

BioCycEcoCyc:EG12031-MONOMER.
ECOL316407:JW2150-MONOMER.

Gene expression databases

GenevestigatorP0A9E9.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP_2.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
[Graphical view]
SMARTSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
PROSITEPS50042. CNMP_BINDING_3. 1 hit.
PS51063. HTH_CRP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0A9E9.

Entry information

Entry nameYEIL_ECOLI
AccessionPrimary (citable) accession number: P0A9E9
Secondary accession number(s): P33023, Q2MAR6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: June 11, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene