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Protein

Regulatory protein YeiL

Gene

yeiL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transcription regulator involved in mid-term, stationary-phase viability under nitrogen starvation. Might control expression of the salvage pathways or in some other way repress the recycling of nucleobases to nucleic acids and enhance their use as general nitrogen sources during nitrogen-limited growth.1 Publication

Miscellaneous

Its activity might be modulated by a sensory iron-sulfur cluster, by reversible dithiol-disulfide formation, or by both mechanisms. It is not an oxygen-responsive regulator.

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi68Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi91Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi93Iron-sulfur (4Fe-4S)Sequence analysis1
Metal bindingi116Iron-sulfur (4Fe-4S)Sequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi158 – 181H-T-H motifPROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: EcoCyc

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12031-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein YeiL
Gene namesi
Name:yeiL
Synonyms:nsr
Ordered Locus Names:b2163, JW2150
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12031. yeiL.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001001821 – 219Regulatory protein YeiLAdd BLAST219

Proteomic databases

PaxDbiP0A9E9.
PRIDEiP0A9E9.

Expressioni

Inductioni

In the stationary phase, aerobic expression is positively autoregulated and significantly dependent on RpoS and Lrp. Its transcription is directly or indirectly repressed by FNR.1 Publication

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4260462. 26 interactors.
STRINGi316385.ECDH10B_2320.

Structurei

3D structure databases

ProteinModelPortaliP0A9E9.
SMRiP0A9E9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini136 – 199HTH crp-typePROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 97Sensory domainSequence analysisAdd BLAST79
Regioni111 – 131Dimer interfaceSequence analysisAdd BLAST21

Phylogenomic databases

eggNOGiENOG4108Y4K. Bacteria.
COG0664. LUCA.
HOGENOMiHOG000094699.
KOiK16326.
PhylomeDBiP0A9E9.

Family and domain databases

CDDicd00038. CAP_ED. 1 hit.
Gene3Di1.10.10.10. 1 hit.
2.60.120.10. 1 hit.
InterProiView protein in InterPro
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR012318. HTH_CRP.
IPR014710. RmlC-like_jellyroll.
IPR036388. WH-like_DNA-bd_sf.
PfamiView protein in Pfam
PF00027. cNMP_binding. 1 hit.
SMARTiView protein in SMART
SM00100. cNMP. 1 hit.
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiView protein in PROSITE
PS50042. CNMP_BINDING_3. 1 hit.
PS51063. HTH_CRP_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A9E9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSESAFKDCF LTDVSADTRL FHFLARDYIV QEGQQPSWLF YLTRGRARLY
60 70 80 90 100
ATLANGRVSL IDFFAAPCFI GEIELIDKDH EPRAVQAIEE CWCLALPMKH
110 120 130 140 150
YRPLLLNDTL FLRKLCVTLS HKNYRNIVSL TQNQSFPLVN RLAAFILLSQ
160 170 180 190 200
EGDLYHEKHT QAAEYLGVSY RHLLYVLAQF IHDGLLIKSK KGYLIKNRKQ
210
LSGLALEMDP ENKFSGMMQ
Length:219
Mass (Da):25,294
Last modified:July 19, 2005 - v1
Checksum:i47720B07A9FD412D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00007 Genomic DNA. Translation: AAA60515.1.
U00096 Genomic DNA. Translation: AAC75224.1.
AP009048 Genomic DNA. Translation: BAE76640.1.
PIRiB64985.
RefSeqiNP_416668.3. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC75224; AAC75224; b2163.
BAE76640; BAE76640; BAE76640.
GeneIDi946670.
KEGGiecj:JW2150.
eco:b2163.
PATRICifig|511145.12.peg.2248.

Similar proteinsi

Entry informationi

Entry nameiYEIL_ECOLI
AccessioniPrimary (citable) accession number: P0A9E9
Secondary accession number(s): P33023, Q2MAR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: October 25, 2017
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene