Reviewed, UniProtKB/Swiss-Prot P0A9E0 (ARAC_ECOLI)

Names and origin

Protein names

Recommended name:
    Arabinose operon regulatory protein

Gene names

Name:	araC
Ordered Locus Names:	b0064, JW0063

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein controls the expression of at least six genes that are involved in the transport and catabolism of L-arabinose. It regulates initiation of transcription of the araBAD operon and it also controls its own synthesis. The L-arabinose operon displays both positive and negative regulation through araC.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Contains 1 HTH araC/xylS-type DNA-binding domain.

Ontologies

Keywords
Biological process	Arabinose catabolism Carbohydrate metabolism Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator Repressor
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	arabinose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 292

292

Arabinose operon regulatory protein

PRO_0000194499

Regions

DNA binding

197 – 216

20

H-T-H motif By similarity

Region

1 – ?

Dimerization

Experimental info

Sequence conflict

7

1

D → E in AAA23468. Ref.7

Secondary structure

1

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292

Helix Strand Turn

Details...

Turn

11 – 13

3

Beta strand

17 – 27

11

Beta strand

36 – 38

3

Beta strand

43 – 53

11

Beta strand

55 – 59

5

Beta strand

62 – 66

5

Beta strand

71 – 74

4

Beta strand

80 – 84

5

Beta strand

88 – 98

11

Helix

102 – 107

6

Beta strand

112 – 114

3

Beta strand

117 – 120

4

Turn

124 – 126

3

Helix

127 – 141

15

Helix

148 – 169

22

Sequences

	Sequence		Length	Mass (Da)
	P0A9E0-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: C5A737E285A4ECC6	FASTA	292	33,384
	10 20 30 40 50 60 MAEAQNDPLL PGYSFNAHLV AGLTPIEANG YLDFFIDRPL GMKGYILNLT IRGQGVVKNQ 70 80 90 100 110 120 GREFVCRPGD ILLFPPGEIH HYGRHPEARE WYHQWVYFRP RAYWHEWLNW PSIFANTGFF 130 140 150 160 170 180 RPDEAHQPHF SDLFGQIINA GQGEGRYSEL LAINLLEQLL LRRMEAINES LHPPMDNRVR 190 200 210 220 230 240 EACQYISDHL ADSNFDIASV AQHVCLSPSR LSHLFRQQLG ISVLSWREDQ RISQAKLLLS 250 260 270 280 290 TTRMPIATVG RNVGFDDQLY FSRVFKKCTG ASPSEFRAGC EEKVNDVAVK LS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The araC gene of Escherichia coli: transcriptional and translational start-points and complete nucleotide sequence." Wallace R.G., Lee N., Fowler A.V. Gene 12:179-190(1980) [PubMed: 7019009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B/R.
[2]	"DNA sequence of the araC regulatory gene from Escherichia coli B/r." Miyada C.G., Horwitz A.H., Cass L.G., Timko J., Wilcox G. Nucleic Acids Res. 8:5267-5274(1980) [PubMed: 7008027] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B/R.
[3]	"Is the amino acid but not the nucleotide sequence of the Escherichia coli araC gene conserved?" Stoner C.M., Schleif R. J. Mol. Biol. 154:649-652(1982) [PubMed: 6283093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[4]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"The araC regulatory gene mRNA contains a leader sequence." Cass L.G., Horwitz A.H., Miyada C.G., Greenfield L., Wilcox G. Mol. Gen. Genet. 180:219-226(1980) [PubMed: 6160371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-61. Strain: B/R.
[8]	"The 1.6 A crystal structure of the AraC sugar-binding and dimerization domain complexed with D-fucose." Soisson S.M., Macdougall-Shackleton B., Schleif R., Wolberger C. J. Mol. Biol. 273:226-237(1997) [PubMed: 9367758] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 1-178.
[9]	"Structural basis for ligand-regulated oligomerization of AraC." Soisson S.M., Macdougall-Shackleton B., Schleif R., Wolberger C. Science 276:421-425(1997) [PubMed: 9103202] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 AND 2.8 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

V00256 Genomic DNA. Translation: CAA23507.1.
V00259 Genomic DNA. Translation: CAA23508.1.
J01641 Genomic DNA. Translation: AAA23466.1.
U00096 Genomic DNA. Translation: AAC73175.1.
AP009048 Genomic DNA. Translation: BAB96633.1.
K01303 Genomic DNA. Translation: AAA23468.1.

PIR

RGECA. A91473.

RefSeq

AP_000728.1.
NP_414606.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XJA	X-ray	2.40	A/B/C/D/E	1-178	[»]
2AAC	X-ray	1.60	A/B	2-178	[»]
2ARA	X-ray	2.80	A	19-167	[»]
2ARC	X-ray	1.50	A/B	7-170	[»]
2K9S	NMR	-	A	175-281	[»]

ModBase

Search...

Protein family/group databases

AraC-XylS

Search...

2-D gel databases

ECO2DBASE

H030.2. 6TH EDITION.

Genome annotation databases

GeneID

944780.

GenomeReviews

Gene locus JW0063 in contig AP009048_GR.
Gene locus b0064 in contig U00096_GR.

KEGG

ecj:JW0063.
eco:b0064.

Organism-specific databases

EchoBASE

EB0052.

EcoGene

EG10054. araC.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A9E0.

OMA

P0A9E0. RNVGFED.

Enzyme and pathway databases

BioCyc

EcoCyc:PD00242.

Family and domain databases

InterPro

IPR003313. AraC_bd.
IPR012287. Homeodomain-rel.
IPR000005. HTH_AraC-typ.
IPR018062. HTH_AraC-typ_sub_2.
IPR018060. HTH_AraC_domain.
[Graphical view]

Gene3D

G3DSA:1.10.10.60. Homeodomain-rel. 2 hits.

Pfam

PF02311. AraC_binding. 1 hit.
PF00165. HTH_AraC. 2 hits.
[Graphical view]

SMART

SM00342. HTH_ARAC. 1 hit.
[Graphical view]

PROSITE

PS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ARAC_ECOLI

Accession

Primary (citable) accession number: P0A9E0
Secondary accession number(s): P03021, Q47056

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)