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Protein

Arabinose operon regulatory protein

Gene

araC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

This protein controls the expression of at least six genes that are involved in the transport and catabolism of L-arabinose. It regulates initiation of transcription of the araBAD operon and it also controls its own synthesis. The L-arabinose operon displays both positive and negative regulation through AraC.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi197 – 216H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

GO - Biological processi

  • arabinose catabolic process Source: EcoliWiki
  • regulation of transcription, DNA-templated Source: GO_Central
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Arabinose catabolism, Carbohydrate metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:PD00242.
ECOL316407:JW0063-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Arabinose operon regulatory protein
Gene namesi
Name:araC
Ordered Locus Names:b0064, JW0063
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10054. araC.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001944991 – 292Arabinose operon regulatory proteinAdd BLAST292

Proteomic databases

PaxDbiP0A9E0.
PRIDEiP0A9E0.

Expressioni

Gene expression databases

CollecTFiEXPREG_000007d0.

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
xerCP0A8P63EBI-1113479,EBI-1133806

Protein-protein interaction databases

BioGridi4260828. 116 interactors.
DIPiDIP-9125N.
IntActiP0A9E0. 12 interactors.
STRINGi511145.b0064.

Structurei

Secondary structure

1292
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni11 – 13Combined sources3
Beta strandi16 – 18Combined sources3
Beta strandi20 – 27Combined sources8
Beta strandi36 – 38Combined sources3
Beta strandi43 – 53Combined sources11
Beta strandi55 – 59Combined sources5
Beta strandi62 – 66Combined sources5
Beta strandi71 – 74Combined sources4
Beta strandi80 – 84Combined sources5
Beta strandi88 – 98Combined sources11
Helixi102 – 107Combined sources6
Beta strandi112 – 114Combined sources3
Beta strandi117 – 120Combined sources4
Turni124 – 126Combined sources3
Helixi127 – 142Combined sources16
Beta strandi145 – 147Combined sources3
Helixi148 – 166Combined sources19
Helixi177 – 188Combined sources12
Helixi197 – 203Combined sources7
Helixi208 – 219Combined sources12
Helixi223 – 241Combined sources19
Helixi246 – 252Combined sources7
Helixi258 – 269Combined sources12
Helixi273 – 278Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XJAX-ray2.40A/B/C/D/E1-178[»]
2AACX-ray1.60A/B2-178[»]
2ARAX-ray2.80A19-167[»]
2ARCX-ray1.50A/B7-170[»]
2K9SNMR-A175-281[»]
ProteinModelPortaliP0A9E0.
SMRiP0A9E0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9E0.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – ?Dimerization

Sequence similaritiesi

Contains 1 HTH araC/xylS-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG41063TF. Bacteria.
ENOG4111G16. LUCA.
HOGENOMiHOG000276603.
InParanoidiP0A9E0.
KOiK02099.
OMAiGFEDQLY.
PhylomeDBiP0A9E0.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
2.60.120.280. 1 hit.
InterProiIPR003313. AraC-bd.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF02311. AraC_binding. 1 hit.
PF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
SSF51215. SSF51215. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9E0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAQNDPLL PGYSFNAHLV AGLTPIEANG YLDFFIDRPL GMKGYILNLT
60 70 80 90 100
IRGQGVVKNQ GREFVCRPGD ILLFPPGEIH HYGRHPEARE WYHQWVYFRP
110 120 130 140 150
RAYWHEWLNW PSIFANTGFF RPDEAHQPHF SDLFGQIINA GQGEGRYSEL
160 170 180 190 200
LAINLLEQLL LRRMEAINES LHPPMDNRVR EACQYISDHL ADSNFDIASV
210 220 230 240 250
AQHVCLSPSR LSHLFRQQLG ISVLSWREDQ RISQAKLLLS TTRMPIATVG
260 270 280 290
RNVGFDDQLY FSRVFKKCTG ASPSEFRAGC EEKVNDVAVK LS
Length:292
Mass (Da):33,384
Last modified:July 21, 1986 - v1
Checksum:iC5A737E285A4ECC6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7D → E in AAA23468 (PubMed:6160371).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00256 Genomic DNA. Translation: CAA23507.1.
V00259 Genomic DNA. Translation: CAA23508.1.
J01641 Genomic DNA. Translation: AAA23466.1.
U00096 Genomic DNA. Translation: AAC73175.1.
AP009048 Genomic DNA. Translation: BAB96633.1.
K01303 Genomic DNA. Translation: AAA23468.1.
PIRiA91473. RGECA.
RefSeqiNP_414606.1. NC_000913.3.
WP_001300811.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73175; AAC73175; b0064.
BAB96633; BAB96633; BAB96633.
GeneIDi944780.
KEGGiecj:JW0063.
eco:b0064.
PATRICi32115229. VBIEscCol129921_0066.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00256 Genomic DNA. Translation: CAA23507.1.
V00259 Genomic DNA. Translation: CAA23508.1.
J01641 Genomic DNA. Translation: AAA23466.1.
U00096 Genomic DNA. Translation: AAC73175.1.
AP009048 Genomic DNA. Translation: BAB96633.1.
K01303 Genomic DNA. Translation: AAA23468.1.
PIRiA91473. RGECA.
RefSeqiNP_414606.1. NC_000913.3.
WP_001300811.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XJAX-ray2.40A/B/C/D/E1-178[»]
2AACX-ray1.60A/B2-178[»]
2ARAX-ray2.80A19-167[»]
2ARCX-ray1.50A/B7-170[»]
2K9SNMR-A175-281[»]
ProteinModelPortaliP0A9E0.
SMRiP0A9E0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260828. 116 interactors.
DIPiDIP-9125N.
IntActiP0A9E0. 12 interactors.
STRINGi511145.b0064.

Proteomic databases

PaxDbiP0A9E0.
PRIDEiP0A9E0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73175; AAC73175; b0064.
BAB96633; BAB96633; BAB96633.
GeneIDi944780.
KEGGiecj:JW0063.
eco:b0064.
PATRICi32115229. VBIEscCol129921_0066.

Organism-specific databases

EchoBASEiEB0052.
EcoGeneiEG10054. araC.

Phylogenomic databases

eggNOGiENOG41063TF. Bacteria.
ENOG4111G16. LUCA.
HOGENOMiHOG000276603.
InParanoidiP0A9E0.
KOiK02099.
OMAiGFEDQLY.
PhylomeDBiP0A9E0.

Enzyme and pathway databases

BioCyciEcoCyc:PD00242.
ECOL316407:JW0063-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A9E0.
PROiP0A9E0.

Gene expression databases

CollecTFiEXPREG_000007d0.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
2.60.120.280. 1 hit.
InterProiIPR003313. AraC-bd.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF02311. AraC_binding. 1 hit.
PF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 2 hits.
SSF51215. SSF51215. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARAC_ECOLI
AccessioniPrimary (citable) accession number: P0A9E0
Secondary accession number(s): P03021, Q47056
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.