Arabinose operon regulatory protein - Escherichia coli (strain K12)

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P0A9E0 (ARAC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 67. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Arabinose operon regulatory protein

Gene names

Name:	araC
Ordered Locus Names:	b0064, JW0063

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein controls the expression of at least six genes that are involved in the transport and catabolism of L-arabinose. It regulates initiation of transcription of the araBAD operon and it also controls its own synthesis. The L-arabinose operon displays both positive and negative regulation through AraC.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Contains 1 HTH araC/xylS-type DNA-binding domain.

Ontologies

Keywords
Biological process	Arabinose catabolism Carbohydrate metabolism Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arabinose catabolic process Inferred from mutant phenotype. Source: EcoliWiki transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 292

292

Arabinose operon regulatory protein

PRO_0000194499

Regions

DNA binding

197 – 216

H-T-H motif By similarity

Region

1 – ?

Dimerization

Experimental info

Sequence conflict

D → E in AAA23468. Ref.7

Secondary structure

292

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A9E0 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: C5A737E285A4ECC6
Show »

FASTA

292

33,384

        10         20         30         40         50         60 
MAEAQNDPLL PGYSFNAHLV AGLTPIEANG YLDFFIDRPL GMKGYILNLT IRGQGVVKNQ 

        70         80         90        100        110        120 
GREFVCRPGD ILLFPPGEIH HYGRHPEARE WYHQWVYFRP RAYWHEWLNW PSIFANTGFF 

       130        140        150        160        170        180 
RPDEAHQPHF SDLFGQIINA GQGEGRYSEL LAINLLEQLL LRRMEAINES LHPPMDNRVR 

       190        200        210        220        230        240 
EACQYISDHL ADSNFDIASV AQHVCLSPSR LSHLFRQQLG ISVLSWREDQ RISQAKLLLS 

       250        260        270        280        290 
TTRMPIATVG RNVGFDDQLY FSRVFKKCTG ASPSEFRAGC EEKVNDVAVK LS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The araC gene of Escherichia coli: transcriptional and translational start-points and complete nucleotide sequence." Wallace R.G., Lee N., Fowler A.V. Gene 12:179-190(1980) [PubMed: 7019009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B/R.
[2]	"DNA sequence of the araC regulatory gene from Escherichia coli B/r." Miyada C.G., Horwitz A.H., Cass L.G., Timko J., Wilcox G. Nucleic Acids Res. 8:5267-5274(1980) [PubMed: 7008027] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: B/R.
[3]	"Is the amino acid but not the nucleotide sequence of the Escherichia coli araC gene conserved?" Stoner C.M., Schleif R. J. Mol. Biol. 154:649-652(1982) [PubMed: 6283093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[4]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"The araC regulatory gene mRNA contains a leader sequence." Cass L.G., Horwitz A.H., Miyada C.G., Greenfield L., Wilcox G. Mol. Gen. Genet. 180:219-226(1980) [PubMed: 6160371] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-61. Strain: B/R.
[8]	"The 1.6 A crystal structure of the AraC sugar-binding and dimerization domain complexed with D-fucose." Soisson S.M., Macdougall-Shackleton B., Schleif R., Wolberger C. J. Mol. Biol. 273:226-237(1997) [PubMed: 9367758] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 1-178.
[9]	"Structural basis for ligand-regulated oligomerization of AraC." Soisson S.M., Macdougall-Shackleton B., Schleif R., Wolberger C. Science 276:421-425(1997) [PubMed: 9103202] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.5 AND 2.8 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V00256 Genomic DNA. Translation: CAA23507.1.
V00259 Genomic DNA. Translation: CAA23508.1.
J01641 Genomic DNA. Translation: AAA23466.1.
U00096 Genomic DNA. Translation: AAC73175.1.
AP009048 Genomic DNA. Translation: BAB96633.1.
K01303 Genomic DNA. Translation: AAA23468.1.

PIR

RGECA. A91473.

RefSeq

NP_414606.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XJA	X-ray	2.40	A/B/C/D/E	1-178	[»]
2AAC	X-ray	1.60	A/B	2-178	[»]
2ARA	X-ray	2.80	A	19-167	[»]
2ARC	X-ray	1.50	A/B	7-170	[»]
2K9S	NMR	-	A	175-281	[»]

ProteinModelPortal

P0A9E0.

SMR

P0A9E0. Positions 7-170, 175-281.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A9E0. 2 interactions.

Protein family/group databases

AraC-XylS

Search...

2D gel databases

ECO2DBASE

H030.2. 6TH EDITION.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000004436; EBESCP00000004436; EBESCG00000003619.
EBESCT00000015482; EBESCP00000014773; EBESCG00000014542.

GeneID

944780.

GenomeReviews

Gene locus JW0063 in contig AP009048_GR.
Gene locus b0064 in contig U00096_GR.

KEGG

ecj:JW0063.
eco:b0064.

PATRIC

32115229. VBIEscCol129921_0066.

Organism-specific databases

EchoBASE

EB0052.

EcoGene

EG10054. araC.

Phylogenomic databases

eggNOG

COG2207.

GeneTree

EBGT00050000008636.

HOGENOM

HBG297197.

OMA

VIEACQF.

PhylomeDB

P0A9E0.

ProtClustDB

PRK10572.

Enzyme and pathway databases

BioCyc

EcoCyc:PD00242.

Gene expression databases

Genevestigator

P0A9E0.

Family and domain databases

InterPro

IPR009057. Homeodomain-like.
IPR012287. Homeodomain-rel.
IPR018062. HTH_AraC-typ_sub_2.
IPR018060. HTH_AraC_DNA-bd.
IPR003313. Tscrpt_reg_HTH_AraC-bd.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]

Gene3D

G3DSA:1.10.10.60. Homeodomain-rel. 2 hits.

K02099.

Pfam

PF02311. AraC_binding. 1 hit.
[Graphical view]

PRINTS

PR00032. HTHARAC.

SMART

SM00342. HTH_ARAC. 1 hit.
[Graphical view]

SUPFAM

SSF51215. AraC_binding. 1 hit.
SSF46689. Homeodomain_like. 2 hits.

PROSITE

PS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ARAC_ECOLI

Accession

Primary (citable) accession number: P0A9E0
Secondary accession number(s): P03021, Q47056

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

2D gel databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents