P0A9D8 (DAPD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
EC=2.3.1.117

Alternative name(s):
Tetrahydrodipicolinate N-succinyltransferase
Short name=THDP succinyltransferase
Short name=THP succinyltransferase
Short name=Tetrahydropicolinate succinylase

Gene names

Name:	dapD
Ordered Locus Names:	b0166, JW0161

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Succinyl-CoA + (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + H2O = CoA + N-succinyl-L-2-amino-6-oxoheptanedioate. HAMAP MF_00811
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route): step 1/3. HAMAP MF_00811
Subunit structure	Homotrimer By similarity. HAMAP MF_00811
Subcellular location	Cytoplasm HAMAP MF_00811.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase HAMAP MF_00811		PRO_0000196933
Sites
	Binding site	104	1	Substrate By similarity
	Binding site	141	1	Substrate By similarity
Experimental info
	Sequence conflict	31	1	V → D in AAA23667. Ref.1
	Sequence conflict	163	1	G → R in AAA23667. Ref.1
	Sequence conflict	177	1	I → M in AAA23667. Ref.1
	Sequence conflict	190	1	V → L in AAA23667. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A9D8 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 42D7A38610DD3AF6	FASTA	274	29,892
	10 20 30 40 50 60 MQQLQNIIET AFERRAEITP ANADTVTREA VNQVIALLDS GALRVAEKID GQWVTHQWLK 70 80 90 100 110 120 KAVLLSFRIN DNQVIEGAES RYFDKVPMKF ADYDEARFQK EGFRVVPPAA VRQGAFIARN 130 140 150 160 170 180 TVLMPSYVNI GAYVDEGTMV DTWATVGSCA QIGKNVHLSG GVGIGGVLEP LQANPTIIED 190 200 210 220 230 240 NCFIGARSEV VEGVIVEEGS VISMGVYIGQ STRIYDRETG EIHYGRVPAG SVVVSGNLPS 250 260 270 KDGKYSLYCA VIVKKVDAKT RGKVGINELL RTID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of expression and nucleotide sequence of the Escherichia coli dapD gene." Richaud C., Richaud F., Martin C., Haziza C., Patte J.-C. J. Biol. Chem. 259:14824-14828(1984) [PubMed: 6094577] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Fujita N., Mori H., Yura T., Ishihama A. Nucleic Acids Res. 22:1637-1639(1994) [PubMed: 8202364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"The genes of the glutamine synthetase adenylylation cascade are not regulated by nitrogen in Escherichia coli." van Heeswijk W.C., Rabenberg M., Westerhoff H.V., Kahn D.D. Mol. Microbiol. 9:443-458(1993) [PubMed: 8412694] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	Pasquali C., Sanchez J.-C., Ravier F., Golaz O., Hughes G.J., Frutiger S., Paquet N., Wilkins M., Appel R.D., Bairoch A., Hochstrasser D.F. Submitted (SEP-1994) to UniProtKB Cited for: PROTEIN SEQUENCE OF 1-11. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]	"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Link A.J., Robison K., Church G.M. Electrophoresis 18:1259-1313(1997) [PubMed: 9298646] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12. Strain: K12 / EMG2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	K02970 Genomic DNA. Translation: AAA23667.1. U70214 Genomic DNA. Translation: AAB08595.1. U00096 Genomic DNA. Translation: AAC73277.1. AP009048 Genomic DNA. Translation: BAB96742.1. Z21842 Genomic DNA. Translation: CAA79888.1.
PIR	XNECSD. F64740.
RefSeq	NP_414708.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0A9D8.
SMR	P0A9D8. Positions 2-274.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-31866N.
IntAct	P0A9D8. 4 interactions.
2D gel databases
SWISS-2DPAGE	P0A9D8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000004043; EBESCP00000004043; EBESCG00000003300. EBESCT00000014470; EBESCP00000013761; EBESCG00000013531.
GeneID	944862.
GenomeReviews	Gene locus JW0161 in contig AP009048_GR. Gene locus b0166 in contig U00096_GR.
KEGG	ecj:JW0161. eco:b0166.
PATRIC	32115439. VBIEscCol129921_0171.
Organism-specific databases
EchoBASE	EB0203.
EcoGene	EG10207. dapD.
Phylogenomic databases
eggNOG	COG2171.
GeneTree	EBGT00050000010899.
HOGENOM	HBG704071.
OMA	KAILLYF.
PhylomeDB	P0A9D8.
ProtClustDB	PRK11830.
Enzyme and pathway databases
BioCyc	EcoCyc:MONOMER0-2001. MetaCyc:MONOMER0-2001.
Gene expression databases
Genevestigator	P0A9D8.
Family and domain databases
HAMAP	MF_00811. DapD. [Tree]
InterPro	IPR005664. DapD. IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR023180. THP_succinylTrfase_dom1. IPR011004. Trimer_LpxA-like. [Graphical view]
Gene3D	G3DSA:1.10.166.10. THP_succinylTrfase_dom1. 1 hit.
KO	K00674.
PANTHER	PTHR19136:SF52. PTHR19136:SF52. 1 hit.
Pfam	PF00132. Hexapep. 2 hits. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR00965. DapD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPD_ECOLI

Accession

Primary (citable) accession number: P0A9D8
Secondary accession number(s): P03948

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 1986
Last sequence update:	July 19, 2005
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program