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Protein

Serine acetyltransferase

Gene

cysE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine.

Enzyme regulationi

Sensitive to feedback inhibition by L-cysteine.

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-cysteine from L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Serine acetyltransferase (cysE)
  2. Cysteine synthase B (cysM), Cysteine synthase A (cysK)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • serine O-acetyltransferase activity Source: EcoCyc

GO - Biological processi

  • cysteine biosynthetic process from serine Source: EcoCyc
  • response to X-ray Source: EcoCyc

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:SERINE-O-ACETTRAN-MONOMER
MetaCyc:SERINE-O-ACETTRAN-MONOMER
BRENDAi2.3.1.30 2026
SABIO-RKiP0A9D4
UniPathwayiUPA00136; UER00199

Names & Taxonomyi

Protein namesi
Recommended name:
Serine acetyltransferase (EC:2.3.1.30)
Short name:
SAT
Gene namesi
Name:cysE
Ordered Locus Names:b3607, JW3582
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10187 cysE

Subcellular locationi

GO - Cellular componenti

  • cysteine synthase complex Source: EcoCyc
  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000686691 – 273Serine acetyltransferaseAdd BLAST273

Proteomic databases

EPDiP0A9D4
PaxDbiP0A9D4
PRIDEiP0A9D4

Interactioni

Subunit structurei

Homohexamer; dimer of a homotrimer (PubMed:10617639). Forms a cysteine synthase complex with 2 copies of CysK (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi4259493, 38 interactors
DIPiDIP-9377N
IntActiP0A9D4, 2 interactors
STRINGi316385.ECDH10B_3789

Structurei

Secondary structure

1273
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 23Combined sources21
Helixi25 – 27Combined sources3
Helixi28 – 34Combined sources7
Turni35 – 37Combined sources3
Helixi41 – 53Combined sources13
Beta strandi56 – 58Combined sources3
Helixi60 – 73Combined sources14
Helixi76 – 91Combined sources16
Helixi99 – 104Combined sources6
Helixi106 – 123Combined sources18
Helixi126 – 140Combined sources15
Beta strandi181 – 183Combined sources3
Beta strandi186 – 188Combined sources3
Beta strandi207 – 211Combined sources5
Beta strandi235 – 237Combined sources3
Turni238 – 241Combined sources4
Beta strandi242 – 245Combined sources4
Beta strandi248 – 250Combined sources3
Helixi252 – 255Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T3DX-ray2.20A/B/C1-273[»]
ProteinModelPortaliP0A9D4
SMRiP0A9D4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9D4

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105D7W Bacteria
COG1045 LUCA
HOGENOMiHOG000049437
InParanoidiP0A9D4
KOiK00640
OMAiHPKIRHG
PhylomeDBiP0A9D4

Family and domain databases

InterProiView protein in InterPro
IPR001451 Hexapep
IPR018357 Hexapep_transf_CS
IPR010493 Ser_AcTrfase_N
IPR005881 Ser_O-AcTrfase
IPR011004 Trimer_LpxA-like_sf
PfamiView protein in Pfam
PF00132 Hexapep, 1 hit
PF06426 SATase_N, 1 hit
PIRSFiPIRSF000441 CysE, 1 hit
SMARTiView protein in SMART
SM00971 SATase_N, 1 hit
SUPFAMiSSF51161 SSF51161, 1 hit
TIGRFAMsiTIGR01172 cysE, 1 hit
PROSITEiView protein in PROSITE
PS00101 HEXAPEP_TRANSFERASES, 1 hit

Sequencei

Sequence statusi: Complete.

P0A9D4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCEELEIVW NNIKAEARTL ADCEPMLASF YHATLLKHEN LGSALSYMLA
60 70 80 90 100
NKLSSPIMPA IAIREVVEEA YAADPEMIAS AACDIQAVRT RDPAVDKYST
110 120 130 140 150
PLLYLKGFHA LQAYRIGHWL WNQGRRALAI FLQNQVSVTF QVDIHPAAKI
160 170 180 190 200
GRGIMLDHAT GIVVGETAVI ENDVSILQSV TLGGTGKSGG DRHPKIREGV
210 220 230 240 250
MIGAGAKILG NIEVGRGAKI GAGSVVLQPV PPHTTAAGVP ARIVGKPDSD
260 270
KPSMDMDQHF NGINHTFEYG DGI
Length:273
Mass (Da):29,317
Last modified:July 19, 2005 - v1
Checksum:i466EB898750EF709
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15745 Genomic DNA Translation: AAA23648.1
M34333 Genomic DNA Translation: AAA23659.1
U00039 Genomic DNA Translation: AAB18584.1
U00096 Genomic DNA Translation: AAC76631.1
AP009048 Genomic DNA Translation: BAE77685.1
PIRiA27896 XYECSA
RefSeqiNP_418064.1, NC_000913.3
WP_001277561.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76631; AAC76631; b3607
BAE77685; BAE77685; BAE77685
GeneIDi948126
KEGGiecj:JW3582
eco:b3607
PATRICifig|1411691.4.peg.3099

Similar proteinsi

Entry informationi

Entry nameiCYSE_ECOLI
AccessioniPrimary (citable) accession number: P0A9D4
Secondary accession number(s): P05796, Q2M7S1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: March 28, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health