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P0A9D4

- CYSE_ECOLI

UniProt

P0A9D4 - CYSE_ECOLI

Protein

Serine acetyltransferase

Gene

cysE

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine.

    Enzyme regulationi

    Sensitive to feedback inhibition by L-cysteine.

    Pathwayi

    GO - Molecular functioni

    1. serine O-acetyltransferase activity Source: EcoCyc

    GO - Biological processi

    1. cysteine biosynthetic process from serine Source: EcoCyc

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Cysteine biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:SERINE-O-ACETTRAN-MONOMER.
    ECOL316407:JW3582-MONOMER.
    MetaCyc:SERINE-O-ACETTRAN-MONOMER.
    RETL1328306-WGS:GSTH-1960-MONOMER.
    RETL1328306-WGS:GSTH-3700-MONOMER.
    BRENDAi2.3.1.30. 2026.
    SABIO-RKP0A9D4.
    UniPathwayiUPA00136; UER00199.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine acetyltransferase (EC:2.3.1.30)
    Short name:
    SAT
    Gene namesi
    Name:cysE
    Ordered Locus Names:b3607, JW3582
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10187. cysE.

    Subcellular locationi

    GO - Cellular componenti

    1. cysteine synthase complex Source: EcoCyc

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 273273Serine acetyltransferasePRO_0000068669Add
    BLAST

    Proteomic databases

    PaxDbiP0A9D4.
    PRIDEiP0A9D4.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A9D4.

    Interactioni

    Subunit structurei

    Homohexamer. Dimer of a homotrimer.1 Publication

    Protein-protein interaction databases

    DIPiDIP-9377N.
    IntActiP0A9D4. 2 interactions.
    STRINGi511145.b3607.

    Structurei

    Secondary structure

    1
    273
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 2321
    Helixi25 – 273
    Helixi28 – 347
    Turni35 – 373
    Helixi41 – 5313
    Beta strandi56 – 583
    Helixi60 – 7314
    Helixi76 – 9116
    Helixi99 – 1046
    Helixi106 – 12318
    Helixi126 – 14015
    Beta strandi181 – 1833
    Beta strandi186 – 1883
    Beta strandi207 – 2115
    Beta strandi235 – 2373
    Turni238 – 2414
    Beta strandi242 – 2454
    Beta strandi248 – 2503
    Helixi252 – 2554

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1T3DX-ray2.20A/B/C1-273[»]
    ProteinModelPortaliP0A9D4.
    SMRiP0A9D4. Positions 1-262.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A9D4.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1045.
    HOGENOMiHOG000049437.
    KOiK00640.
    OMAiRDIQAVC.
    OrthoDBiEOG6HMXK6.
    PhylomeDBiP0A9D4.

    Family and domain databases

    InterProiIPR001451. Hexapep_transf.
    IPR018357. Hexapep_transf_CS.
    IPR010493. Ser_AcTrfase_N.
    IPR005881. Ser_O-AcTrfase.
    IPR011004. Trimer_LpxA-like.
    [Graphical view]
    PfamiPF00132. Hexapep. 1 hit.
    PF06426. SATase_N. 1 hit.
    [Graphical view]
    SMARTiSM00971. SATase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51161. SSF51161. 1 hit.
    TIGRFAMsiTIGR01172. cysE. 1 hit.
    PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A9D4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSCEELEIVW NNIKAEARTL ADCEPMLASF YHATLLKHEN LGSALSYMLA    50
    NKLSSPIMPA IAIREVVEEA YAADPEMIAS AACDIQAVRT RDPAVDKYST 100
    PLLYLKGFHA LQAYRIGHWL WNQGRRALAI FLQNQVSVTF QVDIHPAAKI 150
    GRGIMLDHAT GIVVGETAVI ENDVSILQSV TLGGTGKSGG DRHPKIREGV 200
    MIGAGAKILG NIEVGRGAKI GAGSVVLQPV PPHTTAAGVP ARIVGKPDSD 250
    KPSMDMDQHF NGINHTFEYG DGI 273
    Length:273
    Mass (Da):29,317
    Last modified:July 19, 2005 - v1
    Checksum:i466EB898750EF709
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15745 Genomic DNA. Translation: AAA23648.1.
    M34333 Genomic DNA. Translation: AAA23659.1.
    U00039 Genomic DNA. Translation: AAB18584.1.
    U00096 Genomic DNA. Translation: AAC76631.1.
    AP009048 Genomic DNA. Translation: BAE77685.1.
    PIRiA27896. XYECSA.
    RefSeqiNP_418064.1. NC_000913.3.
    YP_491826.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76631; AAC76631; b3607.
    BAE77685; BAE77685; BAE77685.
    GeneIDi12930499.
    948126.
    KEGGiecj:Y75_p3567.
    eco:b3607.
    PATRICi32122699. VBIEscCol129921_3726.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M15745 Genomic DNA. Translation: AAA23648.1 .
    M34333 Genomic DNA. Translation: AAA23659.1 .
    U00039 Genomic DNA. Translation: AAB18584.1 .
    U00096 Genomic DNA. Translation: AAC76631.1 .
    AP009048 Genomic DNA. Translation: BAE77685.1 .
    PIRi A27896. XYECSA.
    RefSeqi NP_418064.1. NC_000913.3.
    YP_491826.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1T3D X-ray 2.20 A/B/C 1-273 [» ]
    ProteinModelPortali P0A9D4.
    SMRi P0A9D4. Positions 1-262.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9377N.
    IntActi P0A9D4. 2 interactions.
    STRINGi 511145.b3607.

    Proteomic databases

    PaxDbi P0A9D4.
    PRIDEi P0A9D4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76631 ; AAC76631 ; b3607 .
    BAE77685 ; BAE77685 ; BAE77685 .
    GeneIDi 12930499.
    948126.
    KEGGi ecj:Y75_p3567.
    eco:b3607.
    PATRICi 32122699. VBIEscCol129921_3726.

    Organism-specific databases

    EchoBASEi EB0184.
    EcoGenei EG10187. cysE.

    Phylogenomic databases

    eggNOGi COG1045.
    HOGENOMi HOG000049437.
    KOi K00640.
    OMAi RDIQAVC.
    OrthoDBi EOG6HMXK6.
    PhylomeDBi P0A9D4.

    Enzyme and pathway databases

    UniPathwayi UPA00136 ; UER00199 .
    BioCyci EcoCyc:SERINE-O-ACETTRAN-MONOMER.
    ECOL316407:JW3582-MONOMER.
    MetaCyc:SERINE-O-ACETTRAN-MONOMER.
    RETL1328306-WGS:GSTH-1960-MONOMER.
    RETL1328306-WGS:GSTH-3700-MONOMER.
    BRENDAi 2.3.1.30. 2026.
    SABIO-RK P0A9D4.

    Miscellaneous databases

    EvolutionaryTracei P0A9D4.
    PROi P0A9D4.

    Gene expression databases

    Genevestigatori P0A9D4.

    Family and domain databases

    InterProi IPR001451. Hexapep_transf.
    IPR018357. Hexapep_transf_CS.
    IPR010493. Ser_AcTrfase_N.
    IPR005881. Ser_O-AcTrfase.
    IPR011004. Trimer_LpxA-like.
    [Graphical view ]
    Pfami PF00132. Hexapep. 1 hit.
    PF06426. SATase_N. 1 hit.
    [Graphical view ]
    SMARTi SM00971. SATase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51161. SSF51161. 1 hit.
    TIGRFAMsi TIGR01172. cysE. 1 hit.
    PROSITEi PS00101. HEXAPEP_TRANSFERASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "L-cysteine biosynthesis in Escherichia coli: nucleotide sequence and expression of the serine acetyltransferase (cysE) gene from the wild-type and a cysteine-excreting mutant."
      Denk D., Boeck A.
      J. Gen. Microbiol. 133:515-525(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Structure and expression of cysX, the second gene in the Escherichia coli K-12 cysE locus."
      Tei H., Murata K., Kimura A.
      Biochem. Biophys. Res. Commun. 167:948-955(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    3. "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
      Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
      Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "The serine acetyltransferase from Escherichia coli. Over-expression, purification and preliminary crystallographic analysis."
      Wigley D.B., Derrick J.P., Shaw W.V.
      FEBS Lett. 277:267-271(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    7. "Escherichia coli proteome analysis using the gene-protein database."
      VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
      Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY 2D-GEL.
    8. "Serine acetyltransferase from Escherichia coli is a dimer of trimers."
      Hindson V.J., Moody P.C., Rowe A.J., Shaw W.V.
      J. Biol. Chem. 275:461-466(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.

    Entry informationi

    Entry nameiCYSE_ECOLI
    AccessioniPrimary (citable) accession number: P0A9D4
    Secondary accession number(s): P05796, Q2M7S1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3