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Protein

Serine acetyltransferase

Gene

cysE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine.

Enzyme regulationi

Sensitive to feedback inhibition by L-cysteine.

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-cysteine from L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Serine acetyltransferase (cysE)
  2. Cysteine synthase B (cysM), Cysteine synthase A (cysK)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • serine O-acetyltransferase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:SERINE-O-ACETTRAN-MONOMER.
ECOL316407:JW3582-MONOMER.
MetaCyc:SERINE-O-ACETTRAN-MONOMER.
BRENDAi2.3.1.30. 2026.
SABIO-RKP0A9D4.
UniPathwayiUPA00136; UER00199.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine acetyltransferase (EC:2.3.1.30)
Short name:
SAT
Gene namesi
Name:cysE
Ordered Locus Names:b3607, JW3582
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10187. cysE.

Subcellular locationi

GO - Cellular componenti

  • cysteine synthase complex Source: EcoCyc
  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000686691 – 273Serine acetyltransferaseAdd BLAST273

Proteomic databases

EPDiP0A9D4.
PaxDbiP0A9D4.
PRIDEiP0A9D4.

Interactioni

Subunit structurei

Homohexamer; dimer of a homotrimer (PubMed:10617639). Forms a cysteine synthase complex with 2 copies of CysK (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi4259493. 32 interactors.
DIPiDIP-9377N.
IntActiP0A9D4. 2 interactors.
STRINGi511145.b3607.

Structurei

Secondary structure

1273
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 23Combined sources21
Helixi25 – 27Combined sources3
Helixi28 – 34Combined sources7
Turni35 – 37Combined sources3
Helixi41 – 53Combined sources13
Beta strandi56 – 58Combined sources3
Helixi60 – 73Combined sources14
Helixi76 – 91Combined sources16
Helixi99 – 104Combined sources6
Helixi106 – 123Combined sources18
Helixi126 – 140Combined sources15
Beta strandi181 – 183Combined sources3
Beta strandi186 – 188Combined sources3
Beta strandi207 – 211Combined sources5
Beta strandi235 – 237Combined sources3
Turni238 – 241Combined sources4
Beta strandi242 – 245Combined sources4
Beta strandi248 – 250Combined sources3
Helixi252 – 255Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T3DX-ray2.20A/B/C1-273[»]
ProteinModelPortaliP0A9D4.
SMRiP0A9D4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A9D4.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105D7W. Bacteria.
COG1045. LUCA.
HOGENOMiHOG000049437.
InParanoidiP0A9D4.
KOiK00640.
OMAiFHALQSY.
PhylomeDBiP0A9D4.

Family and domain databases

InterProiIPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR010493. Ser_AcTrfase_N.
IPR005881. Ser_O-AcTrfase.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 1 hit.
PF06426. SATase_N. 1 hit.
[Graphical view]
SMARTiSM00971. SATase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01172. cysE. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9D4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSCEELEIVW NNIKAEARTL ADCEPMLASF YHATLLKHEN LGSALSYMLA
60 70 80 90 100
NKLSSPIMPA IAIREVVEEA YAADPEMIAS AACDIQAVRT RDPAVDKYST
110 120 130 140 150
PLLYLKGFHA LQAYRIGHWL WNQGRRALAI FLQNQVSVTF QVDIHPAAKI
160 170 180 190 200
GRGIMLDHAT GIVVGETAVI ENDVSILQSV TLGGTGKSGG DRHPKIREGV
210 220 230 240 250
MIGAGAKILG NIEVGRGAKI GAGSVVLQPV PPHTTAAGVP ARIVGKPDSD
260 270
KPSMDMDQHF NGINHTFEYG DGI
Length:273
Mass (Da):29,317
Last modified:July 19, 2005 - v1
Checksum:i466EB898750EF709
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15745 Genomic DNA. Translation: AAA23648.1.
M34333 Genomic DNA. Translation: AAA23659.1.
U00039 Genomic DNA. Translation: AAB18584.1.
U00096 Genomic DNA. Translation: AAC76631.1.
AP009048 Genomic DNA. Translation: BAE77685.1.
PIRiA27896. XYECSA.
RefSeqiNP_418064.1. NC_000913.3.
WP_001277561.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76631; AAC76631; b3607.
BAE77685; BAE77685; BAE77685.
GeneIDi948126.
KEGGiecj:JW3582.
eco:b3607.
PATRICi32122699. VBIEscCol129921_3726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15745 Genomic DNA. Translation: AAA23648.1.
M34333 Genomic DNA. Translation: AAA23659.1.
U00039 Genomic DNA. Translation: AAB18584.1.
U00096 Genomic DNA. Translation: AAC76631.1.
AP009048 Genomic DNA. Translation: BAE77685.1.
PIRiA27896. XYECSA.
RefSeqiNP_418064.1. NC_000913.3.
WP_001277561.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1T3DX-ray2.20A/B/C1-273[»]
ProteinModelPortaliP0A9D4.
SMRiP0A9D4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259493. 32 interactors.
DIPiDIP-9377N.
IntActiP0A9D4. 2 interactors.
STRINGi511145.b3607.

Proteomic databases

EPDiP0A9D4.
PaxDbiP0A9D4.
PRIDEiP0A9D4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76631; AAC76631; b3607.
BAE77685; BAE77685; BAE77685.
GeneIDi948126.
KEGGiecj:JW3582.
eco:b3607.
PATRICi32122699. VBIEscCol129921_3726.

Organism-specific databases

EchoBASEiEB0184.
EcoGeneiEG10187. cysE.

Phylogenomic databases

eggNOGiENOG4105D7W. Bacteria.
COG1045. LUCA.
HOGENOMiHOG000049437.
InParanoidiP0A9D4.
KOiK00640.
OMAiFHALQSY.
PhylomeDBiP0A9D4.

Enzyme and pathway databases

UniPathwayiUPA00136; UER00199.
BioCyciEcoCyc:SERINE-O-ACETTRAN-MONOMER.
ECOL316407:JW3582-MONOMER.
MetaCyc:SERINE-O-ACETTRAN-MONOMER.
BRENDAi2.3.1.30. 2026.
SABIO-RKP0A9D4.

Miscellaneous databases

EvolutionaryTraceiP0A9D4.
PROiP0A9D4.

Family and domain databases

InterProiIPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR010493. Ser_AcTrfase_N.
IPR005881. Ser_O-AcTrfase.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF00132. Hexapep. 1 hit.
PF06426. SATase_N. 1 hit.
[Graphical view]
SMARTiSM00971. SATase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01172. cysE. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSE_ECOLI
AccessioniPrimary (citable) accession number: P0A9D4
Secondary accession number(s): P05796, Q2M7S1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.