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Protein

Glutathione S-transferase GstA

Gene

gstA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.By similarity

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10GlutathioneBy similarity1
Binding sitei35GlutathioneBy similarity1
Binding sitei52Glutathione; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei99GlutathioneBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciECOO157:GST-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase GstA (EC:2.5.1.18By similarity)
Gene namesi
Name:gstA
Synonyms:gst
Ordered Locus Names:Z2647, ECs2344
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001859711 – 201Glutathione S-transferase GstAAdd BLAST201

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi155864.Z2647.

Structurei

3D structure databases

ProteinModelPortaliP0A9D3.
SMRiP0A9D3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 81GST N-terminalAdd BLAST81
Domaini87 – 201GST C-terminalAdd BLAST115

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni65 – 66Glutathione bindingBy similarity2
Regioni103 – 106Glutathione bindingBy similarity4

Sequence similaritiesi

Belongs to the GST superfamily. Beta family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiENOG4108K3A. Bacteria.
COG0625. LUCA.
HOGENOMiHOG000125748.
KOiK00799.
OMAiSHKLQDG.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13409. GST_N_2. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9D3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLFYKPGAC SLASHITLRE SGKDFTLVSV DLMKKRLENG DDYFAVNPKG
60 70 80 90 100
QVPALLLDDG TLLTEGVAIM QYLADSVPDR QLLAPVNSIS RYKTIEWLNY
110 120 130 140 150
IATELHKGFT PLFRPDTPEE YKPTVRAQLE KKLQYVNEAL KDEHWICGQR
160 170 180 190 200
FTIADAYLFT VLRWAYAVKL NLEGLEHIAA FMQRMAERPE VQDALSAEGL

K
Length:201
Mass (Da):22,868
Last modified:July 19, 2005 - v1
Checksum:i6347401123B044E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56624.1.
BA000007 Genomic DNA. Translation: BAB35767.1.
PIRiD85770.
H90921.
RefSeqiNP_310371.1. NC_002695.1.
WP_000765749.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG56624; AAG56624; Z2647.
BAB35767; BAB35767; BAB35767.
GeneIDi914142.
KEGGiece:Z2647.
ecs:ECs2344.
PATRICi18354016. VBIEscCol44059_2221.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56624.1.
BA000007 Genomic DNA. Translation: BAB35767.1.
PIRiD85770.
H90921.
RefSeqiNP_310371.1. NC_002695.1.
WP_000765749.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliP0A9D3.
SMRiP0A9D3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z2647.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG56624; AAG56624; Z2647.
BAB35767; BAB35767; BAB35767.
GeneIDi914142.
KEGGiece:Z2647.
ecs:ECs2344.
PATRICi18354016. VBIEscCol44059_2221.

Phylogenomic databases

eggNOGiENOG4108K3A. Bacteria.
COG0625. LUCA.
HOGENOMiHOG000125748.
KOiK00799.
OMAiSHKLQDG.

Enzyme and pathway databases

BioCyciECOO157:GST-MONOMER.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF13409. GST_N_2. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTA_ECO57
AccessioniPrimary (citable) accession number: P0A9D3
Secondary accession number(s): P39100
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.