P0A9C4 (GALM_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aldose 1-epimerase
EC=5.1.3.3

Alternative name(s):
Galactose mutarotase
Type-1 mutarotase

Gene names

Name:	galM
Ordered Locus Names:	SF0548, S0556

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose By similarity.
Catalytic activity	Alpha-D-glucose = beta-D-glucose.
Pathway	Carbohydrate metabolism; hexose metabolism.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the aldose epimerase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	galactose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aldose 1-epimerase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Aldose 1-epimerase		PRO_0000197444
Sites
	Active site	175	1	Proton donor By similarity
	Active site	309	1	Proton acceptor By similarity
	Binding site	79	1	Substrate By similarity
	Binding site	245	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A9C4 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 4373732BAEA83E1D	FASTA	346	38,190
	10 20 30 40 50 60 MLNETPALAP DGQPYRLLTL RNNAGMVVTL MDWGATLLSA RIPLSDGSVR EALLGCASPE 70 80 90 100 110 120 CYQDQAAFLG ASIGRYANRI ANSRYTFDGE TVTLSPSQGV NQLHGGPEGF DKRRWQIVNQ 130 140 150 160 170 180 NDRQVLFALS SDDGDQGFPG NLGATVQYRL TDDNRISITY RATVDKPCPV NMTNHVYFNL 190 200 210 220 230 240 DGEQSDVRNH KLQILADEYL PVDEGGIPHD GLKSVAGTSF DFRSAKIIAS EFLADDDQRK 250 260 270 280 290 300 VKGYDHAFLL QAKGDGKKVA AHVWSADEKL QLKVYTTAPA LQFYSGNFLG GTPSRGTEPY 310 320 330 340 ADWQGLALES EFLPDSPNHP EWPQPDCFLR PGEEYSSLTE YQFIAE

References

[1]	"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.
[2]	"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005674 Genomic DNA. Translation: AAN42192.1. AE014073 Genomic DNA. Translation: AAP16065.1.
RefSeq	NP_706485.1. NC_004337.2. NP_836259.1. NC_004741.1.
3D structure databases
ProteinModelPortal	P0A9C4.
SMR	P0A9C4. Positions 3-345.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000088294; EBESCP00000085116; EBESCG00000087339. EBESCT00000090707; EBESCP00000087343; EBESCG00000089751.
GeneID	1023468. 1076990.
GenomeReviews	Gene locus SF0548 in contig AE005674_GR. Gene locus S0556 in contig AE014073_GR.
KEGG	sfl:SF0548. sfx:S0556.
PATRIC	18702181. VBIShiFle31049_0621.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010070.
HOGENOM	HBG703640.
OMA	FNLDGDQ.
ProtClustDB	PRK11055.
Enzyme and pathway databases
BioCyc	SFLE198214:AAN42192.1-MONOMER.
Family and domain databases
InterPro	IPR018052. Ald1_epimerase_CS. IPR013458. Ald_epimerase_bac. IPR008183. Aldose_1-epimerase. IPR015443. Aldose_1-epimerase_subgr. IPR011013. Glyco_hydro-type_carb-bd. IPR014718. Glyco_hydro-type_carb-bd_sub. [Graphical view]
Gene3D	G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit.
KO	K01785.
PANTHER	PTHR10091. Ald_epimerase. 1 hit.
Pfam	PF01263. Aldose_epim. 1 hit. [Graphical view]
SUPFAM	SSF74650. Gal_mut_like. 1 hit.
TIGRFAMs	TIGR02636. GalM_Leloir. 1 hit.
PROSITE	PS00545. ALDOSE_1_EPIMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GALM_SHIFL

Accession

Primary (citable) accession number: P0A9C4
Secondary accession number(s): P40681

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program