P0A9C3 (GALM_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aldose 1-epimerase
EC=5.1.3.3

Alternative name(s):
Galactose mutarotase
Type-1 mutarotase

Gene names

Name:	galM
Ordered Locus Names:	b0756, JW0739

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose.
Catalytic activity	Alpha-D-glucose = beta-D-glucose.
Pathway	Carbohydrate metabolism; hexose metabolism.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the aldose epimerase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	galactose catabolic process via UDP-galactose Inferred from mutant phenotype Ref.1. Source: EcoCyc
Cellular_component	cytoplasm Inferred from direct assay PubMed 5337146. Source: EcoCyc
Molecular_function	aldose 1-epimerase activity Inferred from direct assay PubMed 4945113. Source: EcoCyc carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Aldose 1-epimerase		PRO_0000197443
Sites
	Active site	175	1	Proton donor By similarity
	Active site	309	1	Proton acceptor By similarity
	Binding site	79	1	Substrate By similarity
	Binding site	245	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A9C3 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 4373732BAEA83E1D	FASTA	346	38,190
	10 20 30 40 50 60 MLNETPALAP DGQPYRLLTL RNNAGMVVTL MDWGATLLSA RIPLSDGSVR EALLGCASPE 70 80 90 100 110 120 CYQDQAAFLG ASIGRYANRI ANSRYTFDGE TVTLSPSQGV NQLHGGPEGF DKRRWQIVNQ 130 140 150 160 170 180 NDRQVLFALS SDDGDQGFPG NLGATVQYRL TDDNRISITY RATVDKPCPV NMTNHVYFNL 190 200 210 220 230 240 DGEQSDVRNH KLQILADEYL PVDEGGIPHD GLKSVAGTSF DFRSAKIIAS EFLADDDQRK 250 260 270 280 290 300 VKGYDHAFLL QAKGDGKKVA AHVWSADEKL QLKVYTTAPA LQFYSGNFLG GTPSRGTEPY 310 320 330 340 ADWQGLALES EFLPDSPNHP EWPQPDCFLR PGEEYSSLTE YQFIAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dependence of lactose metabolism upon mutarotase encoded in the gal operon in Escherichia coli." Bouffard G.G., Rudd K.E., Adhya S.L. J. Mol. Biol. 244:269-278(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-14. Strain: K12 / SA1308.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U13636 Genomic DNA. Translation: AAB17020.1. U00096 Genomic DNA. Translation: AAC73843.1. AP009048 Genomic DNA. Translation: BAA35418.1.
PIR	D64811.
RefSeq	NP_415277.1. NC_000913.2. YP_489029.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A9C3.
SMR	P0A9C3. Positions 28-343.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-35901N.
IntAct	P0A9C3. 4 interactions.
MINT	MINT-1249454.
STRING	511145.b0756.
2D gel databases
SWISS-2DPAGE	P0A9C3.
Proteomic databases
PaxDb	P0A9C3.
PRIDE	P0A9C3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73843; AAC73843; b0756. BAA35418; BAA35418; BAA35418.
GeneID	12932716. 944943.
KEGG	ecj:Y75_p0729. eco:b0756.
PATRIC	32116713. VBIEscCol129921_0782.
Organism-specific databases
EchoBASE	EB1649.
EcoGene	EG11698. galM.
Phylogenomic databases
eggNOG	COG2017.
HOGENOM	HOG000072798.
KO	K01785.
OMA	QLHSPDG.
ProtClustDB	PRK11055.
Enzyme and pathway databases
BioCyc	EcoCyc:ALDOSE1EPIM-MONOMER. ECOL316407:JW0739-MONOMER. MetaCyc:ALDOSE1EPIM-MONOMER.
SABIO-RK	P0A9C3.
UniPathway	UPA00242.
Gene expression databases
Genevestigator	P0A9C3.
Family and domain databases
Gene3D	2.70.98.10. 1 hit.
InterPro	IPR018052. Ald1_epimerase_CS. IPR013458. Ald_epimerase_bac. IPR015443. Aldose_1-epimerase. IPR008183. Aldose_1/G6P_1-epimerase. IPR011013. Gal_mutarotase_SF_dom. IPR014718. Glyco_hydro-type_carb-bd_sub. [Graphical view]
PANTHER	PTHR10091. PTHR10091. 1 hit.
Pfam	PF01263. Aldose_epim. 1 hit. [Graphical view]
PIRSF	PIRSF005096. GALM. 1 hit.
SUPFAM	SSF74650. Gal_mut_like. 1 hit.
TIGRFAMs	TIGR02636. galM_Leloir. 1 hit.
PROSITE	PS00545. ALDOSE_1_EPIMERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GALM_ECOLI

Accession

Primary (citable) accession number: P0A9C3
Secondary accession number(s): P40681

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program