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Protein

Aldose 1-epimerase

Gene

galM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose.

Catalytic activityi

Alpha-D-glucose = beta-D-glucose.PROSITE-ProRule annotation

Pathwayi: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei79 – 791SubstrateBy similarity
Active sitei175 – 1751Proton donorPROSITE-ProRule annotation
Binding sitei245 – 2451SubstrateBy similarity
Active sitei309 – 3091Proton acceptorBy similarity

GO - Molecular functioni

  • aldose 1-epimerase activity Source: EcoCyc
  • carbohydrate binding Source: InterPro

GO - Biological processi

  • galactose catabolic process via UDP-galactose Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciEcoCyc:ALDOSE1EPIM-MONOMER.
ECOL316407:JW0739-MONOMER.
MetaCyc:ALDOSE1EPIM-MONOMER.
BRENDAi5.1.3.B1. 2026.
SABIO-RKP0A9C3.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldose 1-epimerase (EC:5.1.3.3)
Alternative name(s):
Galactose mutarotase
Type-1 mutarotase
Gene namesi
Name:galM
Ordered Locus Names:b0756, JW0739
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11698. galM.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 346346Aldose 1-epimerasePRO_0000197443Add
BLAST

Proteomic databases

EPDiP0A9C3.
PaxDbiP0A9C3.
PRIDEiP0A9C3.

2D gel databases

SWISS-2DPAGEP0A9C3.

Interactioni

Protein-protein interaction databases

BioGridi4261710. 6 interactions.
DIPiDIP-35901N.
IntActiP0A9C3. 4 interactions.
MINTiMINT-1249454.
STRINGi511145.b0756.

Structurei

3D structure databases

ProteinModelPortaliP0A9C3.
SMRiP0A9C3. Positions 24-343.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldose epimerase family.Curated

Phylogenomic databases

eggNOGiENOG4105ENN. Bacteria.
COG2017. LUCA.
HOGENOMiHOG000072798.
InParanoidiP0A9C3.
KOiK01785.
OMAiSPNHPDW.
OrthoDBiEOG69WFM5.
PhylomeDBiP0A9C3.

Family and domain databases

Gene3Di2.70.98.10. 1 hit.
InterProiIPR018052. Ald1_epimerase_CS.
IPR013458. Ald_epimerase_bac.
IPR015443. Aldose_1-epimerase.
IPR008183. Aldose_1/G6P_1-epimerase.
IPR011013. Gal_mutarotase_SF_dom.
IPR014718. Glyco_hydro-type_carb-bd_sub.
[Graphical view]
PfamiPF01263. Aldose_epim. 1 hit.
[Graphical view]
PIRSFiPIRSF005096. GALM. 1 hit.
SUPFAMiSSF74650. SSF74650. 1 hit.
TIGRFAMsiTIGR02636. galM_Leloir. 1 hit.
PROSITEiPS00545. ALDOSE_1_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9C3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNETPALAP DGQPYRLLTL RNNAGMVVTL MDWGATLLSA RIPLSDGSVR
60 70 80 90 100
EALLGCASPE CYQDQAAFLG ASIGRYANRI ANSRYTFDGE TVTLSPSQGV
110 120 130 140 150
NQLHGGPEGF DKRRWQIVNQ NDRQVLFALS SDDGDQGFPG NLGATVQYRL
160 170 180 190 200
TDDNRISITY RATVDKPCPV NMTNHVYFNL DGEQSDVRNH KLQILADEYL
210 220 230 240 250
PVDEGGIPHD GLKSVAGTSF DFRSAKIIAS EFLADDDQRK VKGYDHAFLL
260 270 280 290 300
QAKGDGKKVA AHVWSADEKL QLKVYTTAPA LQFYSGNFLG GTPSRGTEPY
310 320 330 340
ADWQGLALES EFLPDSPNHP EWPQPDCFLR PGEEYSSLTE YQFIAE
Length:346
Mass (Da):38,190
Last modified:July 19, 2005 - v1
Checksum:i4373732BAEA83E1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13636 Genomic DNA. Translation: AAB17020.1.
U00096 Genomic DNA. Translation: AAC73843.1.
AP009048 Genomic DNA. Translation: BAA35418.1.
PIRiD64811.
RefSeqiNP_415277.1. NC_000913.3.
WP_000931389.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73843; AAC73843; b0756.
BAA35418; BAA35418; BAA35418.
GeneIDi944943.
KEGGiecj:JW0739.
eco:b0756.
PATRICi32116713. VBIEscCol129921_0782.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13636 Genomic DNA. Translation: AAB17020.1.
U00096 Genomic DNA. Translation: AAC73843.1.
AP009048 Genomic DNA. Translation: BAA35418.1.
PIRiD64811.
RefSeqiNP_415277.1. NC_000913.3.
WP_000931389.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A9C3.
SMRiP0A9C3. Positions 24-343.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261710. 6 interactions.
DIPiDIP-35901N.
IntActiP0A9C3. 4 interactions.
MINTiMINT-1249454.
STRINGi511145.b0756.

2D gel databases

SWISS-2DPAGEP0A9C3.

Proteomic databases

EPDiP0A9C3.
PaxDbiP0A9C3.
PRIDEiP0A9C3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73843; AAC73843; b0756.
BAA35418; BAA35418; BAA35418.
GeneIDi944943.
KEGGiecj:JW0739.
eco:b0756.
PATRICi32116713. VBIEscCol129921_0782.

Organism-specific databases

EchoBASEiEB1649.
EcoGeneiEG11698. galM.

Phylogenomic databases

eggNOGiENOG4105ENN. Bacteria.
COG2017. LUCA.
HOGENOMiHOG000072798.
InParanoidiP0A9C3.
KOiK01785.
OMAiSPNHPDW.
OrthoDBiEOG69WFM5.
PhylomeDBiP0A9C3.

Enzyme and pathway databases

UniPathwayiUPA00242.
BioCyciEcoCyc:ALDOSE1EPIM-MONOMER.
ECOL316407:JW0739-MONOMER.
MetaCyc:ALDOSE1EPIM-MONOMER.
BRENDAi5.1.3.B1. 2026.
SABIO-RKP0A9C3.

Miscellaneous databases

PROiP0A9C3.

Family and domain databases

Gene3Di2.70.98.10. 1 hit.
InterProiIPR018052. Ald1_epimerase_CS.
IPR013458. Ald_epimerase_bac.
IPR015443. Aldose_1-epimerase.
IPR008183. Aldose_1/G6P_1-epimerase.
IPR011013. Gal_mutarotase_SF_dom.
IPR014718. Glyco_hydro-type_carb-bd_sub.
[Graphical view]
PfamiPF01263. Aldose_epim. 1 hit.
[Graphical view]
PIRSFiPIRSF005096. GALM. 1 hit.
SUPFAMiSSF74650. SSF74650. 1 hit.
TIGRFAMsiTIGR02636. galM_Leloir. 1 hit.
PROSITEiPS00545. ALDOSE_1_EPIMERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Dependence of lactose metabolism upon mutarotase encoded in the gal operon in Escherichia coli."
    Bouffard G.G., Rudd K.E., Adhya S.L.
    J. Mol. Biol. 244:269-278(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-14.
    Strain: K12 / SA1308.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiGALM_ECOLI
AccessioniPrimary (citable) accession number: P0A9C3
Secondary accession number(s): P40681
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: March 16, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.