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Protein

Anaerobic glycerol-3-phosphate dehydrogenase subunit A

Gene

glpA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor (By similarity).By similarity

Catalytic activityi

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarity
  • FMNBy similarity

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route).
Proteins known to be involved in this subpathway in this organism are:
  1. Anaerobic glycerol-3-phosphate dehydrogenase subunit A (glpA), Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB), Anaerobic glycerol-3-phosphate dehydrogenase subunit C (glpC)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route), the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 38FADSequence analysisAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciECOO157:GLPA-MONOMER.
UniPathwayiUPA00618; UER00673.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaerobic glycerol-3-phosphate dehydrogenase subunit A (EC:1.1.5.3)
Short name:
G-3-P dehydrogenase
Gene namesi
Name:glpA
Ordered Locus Names:Z3499, ECs3126
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001260941 – 542Anaerobic glycerol-3-phosphate dehydrogenase subunit AAdd BLAST542

Proteomic databases

PRIDEiP0A9C1.

Interactioni

Subunit structurei

Composed of a catalytic GlpA/B dimer and of membrane bound GlpC.By similarity

Protein-protein interaction databases

STRINGi155864.Z3499.

Structurei

3D structure databases

ProteinModelPortaliP0A9C1.
SMRiP0A9C1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4107R5Y. Bacteria.
COG0578. LUCA.
HOGENOMiHOG000004814.
KOiK00111.
OMAiMEGFITI.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
InterProiIPR007419. BFD-like_2Fe2S-bd_dom.
IPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR000447. G3P_DH_FAD-dep.
IPR017752. G3P_DH_GlpA_su.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF04324. Fer2_BFD. 1 hit.
[Graphical view]
PRINTSiPR01001. FADG3PDH.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR03377. glycerol3P_GlpA. 1 hit.
PROSITEiPS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9C1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTRDSQSSD VIIIGGGATG AGIARDCALR GLRVILVERH DIATGATGRN
60 70 80 90 100
HGLLHSGARY AVTDAESARE CISENQILKR IARHCVEPTN GLFITLPEDD
110 120 130 140 150
LSFQATFIRA CEEAGISAEA IDPQQARIIE PAVNPALIGA VKVPDGTVDP
160 170 180 190 200
FRLTAANMLD AKEHGAVILT AHEVTGLIRE GATVCGVRVR NHLTGETQAL
210 220 230 240 250
HAPVVVNAAG IWGQHIAEYA DLRIRMFPAK GSLLIMDHRI NQHVINRCRK
260 270 280 290 300
PSDADILVPG DTISLIGTTS LRIDYNEIDD NRVTAEEVDI LLREGEKLAP
310 320 330 340 350
VMAKTRILRA YSGVRPLVAS DDDPSGRNVS RGIVLLDHAE RDGLDGFITI
360 370 380 390 400
TGGKLMTYRL MAEWATDAVC RKLGNTRPCT TADLALPGSQ EPAEVTLRKV
410 420 430 440 450
ISLPAPLRGS AVYRHGDRTP AWLSEGRLHR SLVCECEAVT AGEVQYAVEN
460 470 480 490 500
LNVNSLLDLR RRTRVGMGTC QGELCACRAA GLLQRFNVTT SAQSIEQLST
510 520 530 540
FLNERWKGVQ PIAWGDALRE SEFTRWVYQG LCGLEKEQKD AL
Length:542
Mass (Da):58,958
Last modified:July 19, 2005 - v1
Checksum:iE5C803F89E912E0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57372.1.
BA000007 Genomic DNA. Translation: BAB36549.1.
PIRiF91019.
RefSeqiNP_311153.1. NC_002695.1.
WP_000857257.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG57372; AAG57372; Z3499.
BAB36549; BAB36549; BAB36549.
GeneIDi916834.
KEGGiece:Z3499.
ecs:ECs3126.
PATRICi18355636. VBIEscCol44059_3019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57372.1.
BA000007 Genomic DNA. Translation: BAB36549.1.
PIRiF91019.
RefSeqiNP_311153.1. NC_002695.1.
WP_000857257.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliP0A9C1.
SMRiP0A9C1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z3499.

Proteomic databases

PRIDEiP0A9C1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG57372; AAG57372; Z3499.
BAB36549; BAB36549; BAB36549.
GeneIDi916834.
KEGGiece:Z3499.
ecs:ECs3126.
PATRICi18355636. VBIEscCol44059_3019.

Phylogenomic databases

eggNOGiENOG4107R5Y. Bacteria.
COG0578. LUCA.
HOGENOMiHOG000004814.
KOiK00111.
OMAiMEGFITI.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00673.
BioCyciECOO157:GLPA-MONOMER.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
InterProiIPR007419. BFD-like_2Fe2S-bd_dom.
IPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR000447. G3P_DH_FAD-dep.
IPR017752. G3P_DH_GlpA_su.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF04324. Fer2_BFD. 1 hit.
[Graphical view]
PRINTSiPR01001. FADG3PDH.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR03377. glycerol3P_GlpA. 1 hit.
PROSITEiPS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLPA_ECO57
AccessioniPrimary (citable) accession number: P0A9C1
Secondary accession number(s): P13032, P78238
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.