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P0A9C0 (GLPA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anaerobic glycerol-3-phosphate dehydrogenase subunit A

Short name=G-3-P dehydrogenase
EC=1.1.5.3
Gene names
Name:glpA
Ordered Locus Names:b2241, JW2235
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length542 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor.

Catalytic activity

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

Cofactor

FAD.

FMN.

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1.

Subunit structure

Composed of a catalytic GlpA/B dimer and of membrane bound GlpC.

Subcellular location

Cell inner membrane; Peripheral membrane protein. Note: Loosely bound to the cytoplasmic membrane often occurring in vesicles associated with fumarate reductase.

Sequence similarities

Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 542542Anaerobic glycerol-3-phosphate dehydrogenase subunit A
PRO_0000126093

Regions

Nucleotide binding10 – 3829FAD Potential

Experimental info

Sequence conflict3291V → L in AAA83864. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P0A9C0 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: E5C803F89E912E0E

FASTA54258,958
        10         20         30         40         50         60 
MKTRDSQSSD VIIIGGGATG AGIARDCALR GLRVILVERH DIATGATGRN HGLLHSGARY 

        70         80         90        100        110        120 
AVTDAESARE CISENQILKR IARHCVEPTN GLFITLPEDD LSFQATFIRA CEEAGISAEA 

       130        140        150        160        170        180 
IDPQQARIIE PAVNPALIGA VKVPDGTVDP FRLTAANMLD AKEHGAVILT AHEVTGLIRE 

       190        200        210        220        230        240 
GATVCGVRVR NHLTGETQAL HAPVVVNAAG IWGQHIAEYA DLRIRMFPAK GSLLIMDHRI 

       250        260        270        280        290        300 
NQHVINRCRK PSDADILVPG DTISLIGTTS LRIDYNEIDD NRVTAEEVDI LLREGEKLAP 

       310        320        330        340        350        360 
VMAKTRILRA YSGVRPLVAS DDDPSGRNVS RGIVLLDHAE RDGLDGFITI TGGKLMTYRL 

       370        380        390        400        410        420 
MAEWATDAVC RKLGNTRPCT TADLALPGSQ EPAEVTLRKV ISLPAPLRGS AVYRHGDRTP 

       430        440        450        460        470        480 
AWLSEGRLHR SLVCECEAVT AGEVQYAVEN LNVNSLLDLR RRTRVGMGTC QGELCACRAA 

       490        500        510        520        530        540 
GLLQRFNVTT SAQSIEQLST FLNERWKGVQ PIAWGDALRE SEFTRWVYQG LCGLEKEQKD 


AL 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and gene-polypeptide relationships of the glpABC operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12."
Cole S.T., Eiglmeier K., Ahmed S., Honore N., Elmes L., Anderson W.F., Weiner J.H.
J. Bacteriol. 170:2448-2456(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
Strain: K12.
[2]"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. expand/collapse author list , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M20938 Genomic DNA. Translation: AAA83864.1.
U00096 Genomic DNA. Translation: AAC75301.1.
AP009048 Genomic DNA. Translation: BAA16060.1.
PIRDEECNA. A32006.
RefSeqNP_416744.1. NC_000913.3.
YP_490480.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0A9C0.
SMRP0A9C0. Positions 5-365.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP0A9C0. 4 interactions.
STRING511145.b2241.

Proteomic databases

PaxDbP0A9C0.
PRIDEP0A9C0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75301; AAC75301; b2241.
BAA16060; BAA16060; BAA16060.
GeneID12930620.
946713.
KEGGecj:Y75_p2203.
eco:b2241.
PATRIC32119841. VBIEscCol129921_2330.

Organism-specific databases

EchoBASEEB0386.
EcoGeneEG10391. glpA.

Phylogenomic databases

eggNOGCOG0578.
HOGENOMHOG000004814.
KOK00111.
OMAQIDNMEV.
OrthoDBEOG651SR7.
PhylomeDBP0A9C0.

Enzyme and pathway databases

BioCycEcoCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER.
ECOL316407:JW2235-MONOMER.
MetaCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER.
UniPathwayUPA00618; UER00673.

Gene expression databases

GenevestigatorP0A9C0.

Family and domain databases

InterProIPR007419. BFD-like_2Fe2S-bd_dom.
IPR006076. FAD-dep_OxRdtase.
IPR000447. G3P_DH_FAD-dep.
IPR017752. G3P_DH_GlpA_su.
[Graphical view]
PfamPF01266. DAO. 1 hit.
PF04324. Fer2_BFD. 1 hit.
[Graphical view]
PRINTSPR01001. FADG3PDH.
TIGRFAMsTIGR03377. glycerol3P_GlpA. 1 hit.
PROSITEPS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0A9C0.

Entry information

Entry nameGLPA_ECOLI
AccessionPrimary (citable) accession number: P0A9C0
Secondary accession number(s): P13032, P78238
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: June 11, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene