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Protein

Anaerobic glycerol-3-phosphate dehydrogenase subunit A

Gene

glpA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor.

Catalytic activityi

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 3829FADSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: EcoCyc
  2. FMN binding Source: InterPro
  3. glycerol-3-phosphate dehydrogenase activity Source: EcoCyc
  4. sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycerol-3-phosphate metabolic process Source: InterPro
  2. glycerol catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER.
ECOL316407:JW2235-MONOMER.
MetaCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER.
UniPathwayiUPA00618; UER00673.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaerobic glycerol-3-phosphate dehydrogenase subunit A (EC:1.1.5.3)
Short name:
G-3-P dehydrogenase
Gene namesi
Name:glpA
Ordered Locus Names:b2241, JW2235
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10391. glpA.

Subcellular locationi

Cell inner membrane; Peripheral membrane protein
Note: Loosely bound to the cytoplasmic membrane often occurring in vesicles associated with fumarate reductase.

GO - Cellular componenti

  1. glycerol-3-phosphate dehydrogenase complex Source: InterPro
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 542542Anaerobic glycerol-3-phosphate dehydrogenase subunit APRO_0000126093Add
BLAST

Proteomic databases

PaxDbiP0A9C0.
PRIDEiP0A9C0.

Expressioni

Gene expression databases

GenevestigatoriP0A9C0.

Interactioni

Subunit structurei

Composed of a catalytic GlpA/B dimer and of membrane bound GlpC.

Protein-protein interaction databases

IntActiP0A9C0. 4 interactions.
STRINGi511145.b2241.

Structurei

3D structure databases

ProteinModelPortaliP0A9C0.
SMRiP0A9C0. Positions 6-365.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0578.
HOGENOMiHOG000004814.
InParanoidiP0A9C0.
KOiK00111.
OMAiMIAMNHR.
OrthoDBiEOG651SR7.
PhylomeDBiP0A9C0.

Family and domain databases

InterProiIPR007419. BFD-like_2Fe2S-bd_dom.
IPR006076. FAD-dep_OxRdtase.
IPR000447. G3P_DH_FAD-dep.
IPR017752. G3P_DH_GlpA_su.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF04324. Fer2_BFD. 1 hit.
[Graphical view]
PRINTSiPR01001. FADG3PDH.
TIGRFAMsiTIGR03377. glycerol3P_GlpA. 1 hit.
PROSITEiPS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9C0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTRDSQSSD VIIIGGGATG AGIARDCALR GLRVILVERH DIATGATGRN
60 70 80 90 100
HGLLHSGARY AVTDAESARE CISENQILKR IARHCVEPTN GLFITLPEDD
110 120 130 140 150
LSFQATFIRA CEEAGISAEA IDPQQARIIE PAVNPALIGA VKVPDGTVDP
160 170 180 190 200
FRLTAANMLD AKEHGAVILT AHEVTGLIRE GATVCGVRVR NHLTGETQAL
210 220 230 240 250
HAPVVVNAAG IWGQHIAEYA DLRIRMFPAK GSLLIMDHRI NQHVINRCRK
260 270 280 290 300
PSDADILVPG DTISLIGTTS LRIDYNEIDD NRVTAEEVDI LLREGEKLAP
310 320 330 340 350
VMAKTRILRA YSGVRPLVAS DDDPSGRNVS RGIVLLDHAE RDGLDGFITI
360 370 380 390 400
TGGKLMTYRL MAEWATDAVC RKLGNTRPCT TADLALPGSQ EPAEVTLRKV
410 420 430 440 450
ISLPAPLRGS AVYRHGDRTP AWLSEGRLHR SLVCECEAVT AGEVQYAVEN
460 470 480 490 500
LNVNSLLDLR RRTRVGMGTC QGELCACRAA GLLQRFNVTT SAQSIEQLST
510 520 530 540
FLNERWKGVQ PIAWGDALRE SEFTRWVYQG LCGLEKEQKD AL
Length:542
Mass (Da):58,958
Last modified:July 19, 2005 - v1
Checksum:iE5C803F89E912E0E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti329 – 3291V → L in AAA83864 (PubMed:3286606).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20938 Genomic DNA. Translation: AAA83864.1.
U00096 Genomic DNA. Translation: AAC75301.1.
AP009048 Genomic DNA. Translation: BAA16060.1.
PIRiA32006. DEECNA.
RefSeqiNP_416744.1. NC_000913.3.
YP_490480.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75301; AAC75301; b2241.
BAA16060; BAA16060; BAA16060.
GeneIDi12930620.
946713.
KEGGiecj:Y75_p2203.
eco:b2241.
PATRICi32119841. VBIEscCol129921_2330.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20938 Genomic DNA. Translation: AAA83864.1.
U00096 Genomic DNA. Translation: AAC75301.1.
AP009048 Genomic DNA. Translation: BAA16060.1.
PIRiA32006. DEECNA.
RefSeqiNP_416744.1. NC_000913.3.
YP_490480.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP0A9C0.
SMRiP0A9C0. Positions 6-365.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP0A9C0. 4 interactions.
STRINGi511145.b2241.

Proteomic databases

PaxDbiP0A9C0.
PRIDEiP0A9C0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75301; AAC75301; b2241.
BAA16060; BAA16060; BAA16060.
GeneIDi12930620.
946713.
KEGGiecj:Y75_p2203.
eco:b2241.
PATRICi32119841. VBIEscCol129921_2330.

Organism-specific databases

EchoBASEiEB0386.
EcoGeneiEG10391. glpA.

Phylogenomic databases

eggNOGiCOG0578.
HOGENOMiHOG000004814.
InParanoidiP0A9C0.
KOiK00111.
OMAiMIAMNHR.
OrthoDBiEOG651SR7.
PhylomeDBiP0A9C0.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00673.
BioCyciEcoCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER.
ECOL316407:JW2235-MONOMER.
MetaCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER.

Miscellaneous databases

PROiP0A9C0.

Gene expression databases

GenevestigatoriP0A9C0.

Family and domain databases

InterProiIPR007419. BFD-like_2Fe2S-bd_dom.
IPR006076. FAD-dep_OxRdtase.
IPR000447. G3P_DH_FAD-dep.
IPR017752. G3P_DH_GlpA_su.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF04324. Fer2_BFD. 1 hit.
[Graphical view]
PRINTSiPR01001. FADG3PDH.
TIGRFAMsiTIGR03377. glycerol3P_GlpA. 1 hit.
PROSITEiPS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and gene-polypeptide relationships of the glpABC operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12."
    Cole S.T., Eiglmeier K., Ahmed S., Honore N., Elmes L., Anderson W.F., Weiner J.H.
    J. Bacteriol. 170:2448-2456(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiGLPA_ECOLI
AccessioniPrimary (citable) accession number: P0A9C0
Secondary accession number(s): P13032, P78238
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: February 4, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.