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P0A9C0

- GLPA_ECOLI

UniProt

P0A9C0 - GLPA_ECOLI

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Protein

Anaerobic glycerol-3-phosphate dehydrogenase subunit A

Gene

glpA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor.

Catalytic activityi

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

Cofactori

FAD.
FMN.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 3829FADSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: EcoCyc
  2. FMN binding Source: InterPro
  3. glycerol-3-phosphate dehydrogenase activity Source: EcoCyc
  4. sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycerol-3-phosphate metabolic process Source: InterPro
  2. glycerol catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciEcoCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER.
ECOL316407:JW2235-MONOMER.
MetaCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER.
UniPathwayiUPA00618; UER00673.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaerobic glycerol-3-phosphate dehydrogenase subunit A (EC:1.1.5.3)
Short name:
G-3-P dehydrogenase
Gene namesi
Name:glpA
Ordered Locus Names:b2241, JW2235
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10391. glpA.

Subcellular locationi

Cell inner membrane; Peripheral membrane protein
Note: Loosely bound to the cytoplasmic membrane often occurring in vesicles associated with fumarate reductase.

GO - Cellular componenti

  1. glycerol-3-phosphate dehydrogenase complex Source: InterPro
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 542542Anaerobic glycerol-3-phosphate dehydrogenase subunit APRO_0000126093Add
BLAST

Proteomic databases

PaxDbiP0A9C0.
PRIDEiP0A9C0.

Expressioni

Gene expression databases

GenevestigatoriP0A9C0.

Interactioni

Subunit structurei

Composed of a catalytic GlpA/B dimer and of membrane bound GlpC.

Protein-protein interaction databases

IntActiP0A9C0. 4 interactions.
STRINGi511145.b2241.

Structurei

3D structure databases

ProteinModelPortaliP0A9C0.
SMRiP0A9C0. Positions 31-365.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0578.
HOGENOMiHOG000004814.
InParanoidiP0A9C0.
KOiK00111.
OMAiQIDNMEV.
OrthoDBiEOG651SR7.
PhylomeDBiP0A9C0.

Family and domain databases

InterProiIPR007419. BFD-like_2Fe2S-bd_dom.
IPR006076. FAD-dep_OxRdtase.
IPR000447. G3P_DH_FAD-dep.
IPR017752. G3P_DH_GlpA_su.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF04324. Fer2_BFD. 1 hit.
[Graphical view]
PRINTSiPR01001. FADG3PDH.
TIGRFAMsiTIGR03377. glycerol3P_GlpA. 1 hit.
PROSITEiPS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A9C0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTRDSQSSD VIIIGGGATG AGIARDCALR GLRVILVERH DIATGATGRN
60 70 80 90 100
HGLLHSGARY AVTDAESARE CISENQILKR IARHCVEPTN GLFITLPEDD
110 120 130 140 150
LSFQATFIRA CEEAGISAEA IDPQQARIIE PAVNPALIGA VKVPDGTVDP
160 170 180 190 200
FRLTAANMLD AKEHGAVILT AHEVTGLIRE GATVCGVRVR NHLTGETQAL
210 220 230 240 250
HAPVVVNAAG IWGQHIAEYA DLRIRMFPAK GSLLIMDHRI NQHVINRCRK
260 270 280 290 300
PSDADILVPG DTISLIGTTS LRIDYNEIDD NRVTAEEVDI LLREGEKLAP
310 320 330 340 350
VMAKTRILRA YSGVRPLVAS DDDPSGRNVS RGIVLLDHAE RDGLDGFITI
360 370 380 390 400
TGGKLMTYRL MAEWATDAVC RKLGNTRPCT TADLALPGSQ EPAEVTLRKV
410 420 430 440 450
ISLPAPLRGS AVYRHGDRTP AWLSEGRLHR SLVCECEAVT AGEVQYAVEN
460 470 480 490 500
LNVNSLLDLR RRTRVGMGTC QGELCACRAA GLLQRFNVTT SAQSIEQLST
510 520 530 540
FLNERWKGVQ PIAWGDALRE SEFTRWVYQG LCGLEKEQKD AL
Length:542
Mass (Da):58,958
Last modified:July 19, 2005 - v1
Checksum:iE5C803F89E912E0E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti329 – 3291V → L in AAA83864. (PubMed:3286606)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20938 Genomic DNA. Translation: AAA83864.1.
U00096 Genomic DNA. Translation: AAC75301.1.
AP009048 Genomic DNA. Translation: BAA16060.1.
PIRiA32006. DEECNA.
RefSeqiNP_416744.1. NC_000913.3.
YP_490480.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75301; AAC75301; b2241.
BAA16060; BAA16060; BAA16060.
GeneIDi12930620.
946713.
KEGGiecj:Y75_p2203.
eco:b2241.
PATRICi32119841. VBIEscCol129921_2330.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M20938 Genomic DNA. Translation: AAA83864.1 .
U00096 Genomic DNA. Translation: AAC75301.1 .
AP009048 Genomic DNA. Translation: BAA16060.1 .
PIRi A32006. DEECNA.
RefSeqi NP_416744.1. NC_000913.3.
YP_490480.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0A9C0.
SMRi P0A9C0. Positions 31-365.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P0A9C0. 4 interactions.
STRINGi 511145.b2241.

Proteomic databases

PaxDbi P0A9C0.
PRIDEi P0A9C0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75301 ; AAC75301 ; b2241 .
BAA16060 ; BAA16060 ; BAA16060 .
GeneIDi 12930620.
946713.
KEGGi ecj:Y75_p2203.
eco:b2241.
PATRICi 32119841. VBIEscCol129921_2330.

Organism-specific databases

EchoBASEi EB0386.
EcoGenei EG10391. glpA.

Phylogenomic databases

eggNOGi COG0578.
HOGENOMi HOG000004814.
InParanoidi P0A9C0.
KOi K00111.
OMAi QIDNMEV.
OrthoDBi EOG651SR7.
PhylomeDBi P0A9C0.

Enzyme and pathway databases

UniPathwayi UPA00618 ; UER00673 .
BioCyci EcoCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER.
ECOL316407:JW2235-MONOMER.
MetaCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER.

Miscellaneous databases

PROi P0A9C0.

Gene expression databases

Genevestigatori P0A9C0.

Family and domain databases

InterProi IPR007419. BFD-like_2Fe2S-bd_dom.
IPR006076. FAD-dep_OxRdtase.
IPR000447. G3P_DH_FAD-dep.
IPR017752. G3P_DH_GlpA_su.
[Graphical view ]
Pfami PF01266. DAO. 1 hit.
PF04324. Fer2_BFD. 1 hit.
[Graphical view ]
PRINTSi PR01001. FADG3PDH.
TIGRFAMsi TIGR03377. glycerol3P_GlpA. 1 hit.
PROSITEi PS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and gene-polypeptide relationships of the glpABC operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12."
    Cole S.T., Eiglmeier K., Ahmed S., Honore N., Elmes L., Anderson W.F., Weiner J.H.
    J. Bacteriol. 170:2448-2456(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiGLPA_ECOLI
AccessioniPrimary (citable) accession number: P0A9C0
Secondary accession number(s): P13032, P78238
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3