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P0A9C0

- GLPA_ECOLI

UniProt

P0A9C0 - GLPA_ECOLI

Protein

Anaerobic glycerol-3-phosphate dehydrogenase subunit A

Gene

glpA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor.

    Catalytic activityi

    sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

    Cofactori

    FAD.
    FMN.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 3829FADSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: EcoCyc
    2. FMN binding Source: InterPro
    3. glycerol-3-phosphate dehydrogenase activity Source: EcoCyc
    4. sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycerol-3-phosphate metabolic process Source: InterPro
    2. glycerol catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciEcoCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER.
    ECOL316407:JW2235-MONOMER.
    MetaCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER.
    UniPathwayiUPA00618; UER00673.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anaerobic glycerol-3-phosphate dehydrogenase subunit A (EC:1.1.5.3)
    Short name:
    G-3-P dehydrogenase
    Gene namesi
    Name:glpA
    Ordered Locus Names:b2241, JW2235
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10391. glpA.

    Subcellular locationi

    Cell inner membrane; Peripheral membrane protein
    Note: Loosely bound to the cytoplasmic membrane often occurring in vesicles associated with fumarate reductase.

    GO - Cellular componenti

    1. glycerol-3-phosphate dehydrogenase complex Source: InterPro
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 542542Anaerobic glycerol-3-phosphate dehydrogenase subunit APRO_0000126093Add
    BLAST

    Proteomic databases

    PaxDbiP0A9C0.
    PRIDEiP0A9C0.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A9C0.

    Interactioni

    Subunit structurei

    Composed of a catalytic GlpA/B dimer and of membrane bound GlpC.

    Protein-protein interaction databases

    IntActiP0A9C0. 4 interactions.
    STRINGi511145.b2241.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A9C0.
    SMRiP0A9C0. Positions 5-365.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0578.
    HOGENOMiHOG000004814.
    KOiK00111.
    OMAiQIDNMEV.
    OrthoDBiEOG651SR7.
    PhylomeDBiP0A9C0.

    Family and domain databases

    InterProiIPR007419. BFD-like_2Fe2S-bd_dom.
    IPR006076. FAD-dep_OxRdtase.
    IPR000447. G3P_DH_FAD-dep.
    IPR017752. G3P_DH_GlpA_su.
    [Graphical view]
    PfamiPF01266. DAO. 1 hit.
    PF04324. Fer2_BFD. 1 hit.
    [Graphical view]
    PRINTSiPR01001. FADG3PDH.
    TIGRFAMsiTIGR03377. glycerol3P_GlpA. 1 hit.
    PROSITEiPS00977. FAD_G3PDH_1. 1 hit.
    PS00978. FAD_G3PDH_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A9C0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTRDSQSSD VIIIGGGATG AGIARDCALR GLRVILVERH DIATGATGRN    50
    HGLLHSGARY AVTDAESARE CISENQILKR IARHCVEPTN GLFITLPEDD 100
    LSFQATFIRA CEEAGISAEA IDPQQARIIE PAVNPALIGA VKVPDGTVDP 150
    FRLTAANMLD AKEHGAVILT AHEVTGLIRE GATVCGVRVR NHLTGETQAL 200
    HAPVVVNAAG IWGQHIAEYA DLRIRMFPAK GSLLIMDHRI NQHVINRCRK 250
    PSDADILVPG DTISLIGTTS LRIDYNEIDD NRVTAEEVDI LLREGEKLAP 300
    VMAKTRILRA YSGVRPLVAS DDDPSGRNVS RGIVLLDHAE RDGLDGFITI 350
    TGGKLMTYRL MAEWATDAVC RKLGNTRPCT TADLALPGSQ EPAEVTLRKV 400
    ISLPAPLRGS AVYRHGDRTP AWLSEGRLHR SLVCECEAVT AGEVQYAVEN 450
    LNVNSLLDLR RRTRVGMGTC QGELCACRAA GLLQRFNVTT SAQSIEQLST 500
    FLNERWKGVQ PIAWGDALRE SEFTRWVYQG LCGLEKEQKD AL 542
    Length:542
    Mass (Da):58,958
    Last modified:July 19, 2005 - v1
    Checksum:iE5C803F89E912E0E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti329 – 3291V → L in AAA83864. (PubMed:3286606)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20938 Genomic DNA. Translation: AAA83864.1.
    U00096 Genomic DNA. Translation: AAC75301.1.
    AP009048 Genomic DNA. Translation: BAA16060.1.
    PIRiA32006. DEECNA.
    RefSeqiNP_416744.1. NC_000913.3.
    YP_490480.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75301; AAC75301; b2241.
    BAA16060; BAA16060; BAA16060.
    GeneIDi12930620.
    946713.
    KEGGiecj:Y75_p2203.
    eco:b2241.
    PATRICi32119841. VBIEscCol129921_2330.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20938 Genomic DNA. Translation: AAA83864.1 .
    U00096 Genomic DNA. Translation: AAC75301.1 .
    AP009048 Genomic DNA. Translation: BAA16060.1 .
    PIRi A32006. DEECNA.
    RefSeqi NP_416744.1. NC_000913.3.
    YP_490480.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0A9C0.
    SMRi P0A9C0. Positions 5-365.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P0A9C0. 4 interactions.
    STRINGi 511145.b2241.

    Proteomic databases

    PaxDbi P0A9C0.
    PRIDEi P0A9C0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75301 ; AAC75301 ; b2241 .
    BAA16060 ; BAA16060 ; BAA16060 .
    GeneIDi 12930620.
    946713.
    KEGGi ecj:Y75_p2203.
    eco:b2241.
    PATRICi 32119841. VBIEscCol129921_2330.

    Organism-specific databases

    EchoBASEi EB0386.
    EcoGenei EG10391. glpA.

    Phylogenomic databases

    eggNOGi COG0578.
    HOGENOMi HOG000004814.
    KOi K00111.
    OMAi QIDNMEV.
    OrthoDBi EOG651SR7.
    PhylomeDBi P0A9C0.

    Enzyme and pathway databases

    UniPathwayi UPA00618 ; UER00673 .
    BioCyci EcoCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER.
    ECOL316407:JW2235-MONOMER.
    MetaCyc:ANGLYC3PDEHYDROGSUBUNITA-MONOMER.

    Miscellaneous databases

    PROi P0A9C0.

    Gene expression databases

    Genevestigatori P0A9C0.

    Family and domain databases

    InterProi IPR007419. BFD-like_2Fe2S-bd_dom.
    IPR006076. FAD-dep_OxRdtase.
    IPR000447. G3P_DH_FAD-dep.
    IPR017752. G3P_DH_GlpA_su.
    [Graphical view ]
    Pfami PF01266. DAO. 1 hit.
    PF04324. Fer2_BFD. 1 hit.
    [Graphical view ]
    PRINTSi PR01001. FADG3PDH.
    TIGRFAMsi TIGR03377. glycerol3P_GlpA. 1 hit.
    PROSITEi PS00977. FAD_G3PDH_1. 1 hit.
    PS00978. FAD_G3PDH_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and gene-polypeptide relationships of the glpABC operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12."
      Cole S.T., Eiglmeier K., Ahmed S., Honore N., Elmes L., Anderson W.F., Weiner J.H.
      J. Bacteriol. 170:2448-2456(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
      Strain: K12.
    2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiGLPA_ECOLI
    AccessioniPrimary (citable) accession number: P0A9C0
    Secondary accession number(s): P13032, P78238
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3