Reviewed, UniProtKB/Swiss-Prot P0A9B4 (G3P1_ECO57)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase A
      Short name=GAPDH-A
    EC=1.2.1.12

Gene names

Name:	gapA
Ordered Locus Names:	Z2818, ECs2488

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 331	330	Glyceraldehyde-3-phosphate dehydrogenase A		PRO_0000145651
Regions
	Nucleotide binding	12 – 13	2	NAD By similarity
	Region	149 – 151	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	209 – 210	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	150	1	Nucleophile By similarity
	Binding site	34	1	NAD By similarity
	Binding site	78	1	NAD; via carbonyl oxygen By similarity
	Binding site	180	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	232	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	314	1	NAD By similarity
	Site	177	1	Activates thiol group during catalysis By similarity
Amino acid modifications
	Modified residue	132	1	N6-acetyllysine By similarity
	Modified residue	138	1	N6-acetyllysine By similarity
	Modified residue	192	1	N6-acetyllysine By similarity
	Modified residue	249	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A9B4-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: B3A460AA6D59E46D	FASTA	331	35,532
	10 20 30 40 50 60 MTIKVGINGF GRIGRIVFRA AQKRSDIEIV AINDLLDADY MAYMLKYDST HGRFDGTVEV 70 80 90 100 110 120 KDGHLIVNGK KIRVTAERDP ANLKWDEVGV DVVAEATGLF LTDETARKHI TAGAKKVVMT 130 140 150 160 170 180 GPSKDNTPMF VKGANFDKYA GQDIVSNASC TTNCLAPLAK VINDNFGIIE GLMTTVHATT 190 200 210 220 230 240 ATQKTVDGPS HKDWRGGRGA SQNIIPSSTG AAKAVGKVLP ELNGKLTGMA FRVPTPNVSV 250 260 270 280 290 300 VDLTVRLEKA ATYEQIKAAV KAAAEGEMKG VLGYTEDDVV STDFNGEVCT SVFDAKAGIA 310 320 330 LNDNFVKLVS WYDNETGYSN KVLDLIAHIS K

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG56768.1. BA000007 Genomic DNA. Translation: BAB35911.1.
PIR	D85788. H90939.
RefSeq	NP_288215.1. NP_310515.1.
3D structure databases
SMR	P0A9B4. Positions 2-331.
ModBase	Search...
Genome annotation databases
GeneID	913285. 961753.
GenomeReviews	Gene locus Z2818 in contig AE005174_GR. Gene locus ECs2488 in contig BA000007_GR.
KEGG	ece:Z2818. ecs:ECs2488.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A9B4.
OMA	P0A9B4. KEICTEV.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS2488-MON.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DH. IPR006424. Glyceraldehyde-3-P_DH_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P1_ECO57

Accession

Primary (citable) accession number: P0A9B4
Secondary accession number(s): P06977

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)