ID FUR_ECOLI Reviewed; 148 AA. AC P0A9A9; P06975; DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot. DT 01-APR-1988, sequence version 1. DT 16-JUN-2009, entry version 43. DE RecName: Full=Ferric uptake regulation protein; DE Short=Ferric uptake regulator; GN Name=fur; OrderedLocusNames=b0683, JW0669; OS Escherichia coli (strain K12). OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OX NCBI_TaxID=83333; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RX MEDLINE=85295483; PubMed=2993806; DOI=10.1007/BF00383321; RA Schaeffer S., Hantke K., Braun V.; RT "Nucleotide sequence of the iron regulatory gene fur."; RL Mol. Gen. Genet. 200:110-113(1985). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX MEDLINE=97061202; PubMed=8905232; DOI=10.1093/dnares/3.3.137; RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., RA Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., RA Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., RA Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., RA Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., RA Yano M., Horiuchi T.; RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome RT corresponding to the 12.7-28.0 min region on the linkage map."; RL DNA Res. 3:137-155(1996). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / MG1655 / ATCC 47076; RX MEDLINE=97426617; PubMed=9278503; DOI=10.1126/science.277.5331.1453; RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., RA Mau B., Shao Y.; RT "The complete genome sequence of Escherichia coli K-12."; RL Science 277:1453-1474(1997). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911; RX PubMed=16738553; DOI=10.1038/msb4100049; RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.; RT "Highly accurate genome sequences of Escherichia coli K-12 strains RT MG1655 and W3110."; RL Mol. Syst. Biol. 2:E1-E5(2006). RN [5] RP FUNCTION. RX MEDLINE=88050831; PubMed=2823881; DOI=10.1021/bi00391a039; RA Bagg A., Neilands J.B.; RT "Ferric uptake regulation protein acts as a repressor, employing iron RT (II) as a cofactor to bind the operator of an iron transport operon in RT Escherichia coli."; RL Biochemistry 26:5471-5477(1987). RN [6] RP STRUCTURAL DYNAMICS. RX MEDLINE=91329402; PubMed=1868094; DOI=10.1021/bi00247a016; RA Coy M., Neilands J.B.; RT "Structural dynamics and functional domains of the fur protein."; RL Biochemistry 30:8201-8210(1991). RN [7] RP ZINC-BINDING SITES. RX MEDLINE=99315237; PubMed=10387106; DOI=10.1021/bi9902283; RA Gonzalez de Peredo A., Saint-Pierre C., Adrait A., Jacquamet L., RA Latour J.M., Michaud-Soret I., Forest E.; RT "Identification of the two zinc-bound cysteines in the ferric uptake RT regulation protein from Escherichia coli: chemical modification and RT mass spectrometry analysis."; RL Biochemistry 38:8582-8589(1999). RN [8] RP STRUCTURE BY NMR. RX MEDLINE=91200060; PubMed=2015825; RX DOI=10.1111/j.1432-1033.1991.tb15880.x; RA Saito T., Williams R.J.; RT "The binding of the ferric uptake regulation protein to a DNA RT fragment."; RL Eur. J. Biochem. 197:43-47(1991). CC -!- FUNCTION: Acts as a global negative controlling element, employing CC Fe(2+) as a cofactor to bind the operator of the repressed genes. CC Regulates the expression of several outer-membrane proteins CC including the iron transport operon. CC -!- SUBUNIT: Homodimer (By similarity). CC -!- INTERACTION: CC P18843:nadE; NbExp=1; IntAct=EBI-369487, EBI-548960; CC -!- SUBCELLULAR LOCATION: Cytoplasm. CC -!- MISCELLANEOUS: Activated by cadmium, cobalt, copper, and manganese CC ions, but not zinc ions. CC -!- SIMILARITY: Belongs to the Fur family. CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; X02589; CAA26429.1; -; Genomic_DNA. DR EMBL; U00096; AAC73777.1; -; Genomic_DNA. DR EMBL; AP009048; BAA35331.1; -; Genomic_DNA. DR PIR; S07308; S07308. DR RefSeq; AP_001321.1; -. DR RefSeq; NP_415209.1; -. DR PDB; 2FU4; X-ray; 1.80 A; A/B=1-83. DR PDBsum; 2FU4; -. DR IntAct; P0A9A9; 50. DR SWISS-2DPAGE; P0A9A9; -. DR ECO2DBASE; G015.8; 6TH EDITION. DR GeneID; 945295; -. DR GenomeReviews; AP009048_GR; JW0669. DR GenomeReviews; U00096_GR; b0683. DR KEGG; ecj:JW0669; -. DR KEGG; eco:b0683; -. DR EchoBASE; EB0354; -. DR EcoGene; EG10359; fur. DR HOGENOM; P0A9A9; -. DR OMA; P0A9A9; GKHHDHL. DR BioCyc; EcoCyc:PD00260; -. DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW. DR GO; GO:0005515; F:protein binding; IPI:IntAct. DR GO; GO:0003700; F:transcription factor activity; IEA:InterPro. DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW. DR GO; GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW. DR GO; GO:0006350; P:transcription; IEA:UniProtKB-KW. DR InterPro; IPR002481; FUR. DR Pfam; PF01475; FUR; 1. DR ProDom; PD002003; FUR; 1. PE 1: Evidence at protein level; KW 3D-structure; Complete proteome; Cytoplasm; DNA-binding; Iron; KW Metal-binding; Repressor; Transcription; Transcription regulation; KW Zinc. FT CHAIN 1 148 Ferric uptake regulation protein. FT /FTId=PRO_0000095550. FT REGION 1 84 DNA-binding (By similarity). FT REGION 85 148 Dimerization (By similarity). FT COMPBIAS 86 90 His-rich. FT METAL 33 33 Zinc (By similarity). FT METAL 81 81 Zinc (By similarity). FT METAL 87 87 Iron (By similarity). FT METAL 89 89 Iron (By similarity). FT METAL 90 90 Zinc (By similarity). FT METAL 93 93 Zinc. FT METAL 96 96 Zinc. FT METAL 101 101 Zinc (By similarity). FT METAL 108 108 Iron (By similarity). FT METAL 125 125 Iron (By similarity). FT HELIX 4 10 FT HELIX 17 26 FT HELIX 29 31 FT STRAND 32 34 FT HELIX 36 45 FT HELIX 52 64 FT STRAND 67 72 FT HELIX 74 76 FT STRAND 78 82 SQ SEQUENCE 148 AA; 16795 MW; B868360513489DD3 CRC64; MTDNNTALKK AGLKVTLPRL KILEVLQEPD NHHVSAEDLY KRLIDMGEEI GLATVYRVLN QFDDAGIVTR HNFEGGKSVF ELTQQHHHDH LICLDCGKVI EFSDDSIEAR QREIAAKHGI RLTNHSLYLY GHCAEGDCRE DEHAHEGK //