Ferric uptake regulation protein - Escherichia coli (strain K12)

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P0A9A9 (FUR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 68. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Ferric uptake regulation protein
Short name=Ferric uptake regulator

Gene names

Name:	fur
Ordered Locus Names:	b0683, JW0669

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a global negative controlling element, employing Fe²⁺ as a cofactor to bind the operator of the repressed genes. Regulates the expression of several outer-membrane proteins including the iron transport operon. Ref.5
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	Activated by cadmium, cobalt, copper, and manganese ions, but not zinc ions.
Sequence similarities	Belongs to the Fur family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	DNA-binding Iron Metal-binding Zinc
Molecular function	Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from direct assay. Source: EcoliWiki
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nadE	P18843	1	EBI-369487,EBI-548960

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 148

148

Ferric uptake regulation protein

PRO_0000095550

Regions

Region

1 – 84

DNA-binding By similarity

Region

85 – 148

Dimerization By similarity

Compositional bias

86 – 90

His-rich

Sites

Metal binding

Zinc By similarity

Metal binding

Zinc By similarity

Metal binding

Iron By similarity

Metal binding

Iron By similarity

Metal binding

Zinc By similarity

Metal binding

Zinc

Metal binding

Zinc

Metal binding

101

Zinc By similarity

Metal binding

108

Iron By similarity

Metal binding

125

Iron By similarity

Secondary structure

148

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A9A9 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: B868360513489DD3
Show »

FASTA

148

16,795

        10         20         30         40         50         60 
MTDNNTALKK AGLKVTLPRL KILEVLQEPD NHHVSAEDLY KRLIDMGEEI GLATVYRVLN 

        70         80         90        100        110        120 
QFDDAGIVTR HNFEGGKSVF ELTQQHHHDH LICLDCGKVI EFSDDSIEAR QREIAAKHGI 

       130        140 
RLTNHSLYLY GHCAEGDCRE DEHAHEGK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the iron regulatory gene fur." Schaeffer S., Hantke K., Braun V. Mol. Gen. Genet. 200:110-113(1985) [PubMed: 2993806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Ferric uptake regulation protein acts as a repressor, employing iron (II) as a cofactor to bind the operator of an iron transport operon in Escherichia coli." Bagg A., Neilands J.B. Biochemistry 26:5471-5477(1987) [PubMed: 2823881] [Abstract] Cited for: FUNCTION.
[6]	"Structural dynamics and functional domains of the fur protein." Coy M., Neilands J.B. Biochemistry 30:8201-8210(1991) [PubMed: 1868094] [Abstract] Cited for: STRUCTURAL DYNAMICS.
[7]	"Identification of the two zinc-bound cysteines in the ferric uptake regulation protein from Escherichia coli: chemical modification and mass spectrometry analysis." Gonzalez de Peredo A., Saint-Pierre C., Adrait A., Jacquamet L., Latour J.M., Michaud-Soret I., Forest E. Biochemistry 38:8582-8589(1999) [PubMed: 10387106] [Abstract] Cited for: ZINC-BINDING SITES.
[8]	"The binding of the ferric uptake regulation protein to a DNA fragment." Saito T., Williams R.J. Eur. J. Biochem. 197:43-47(1991) [PubMed: 2015825] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X02589 Genomic DNA. Translation: CAA26429.1.
U00096 Genomic DNA. Translation: AAC73777.1.
AP009048 Genomic DNA. Translation: BAA35331.1.

PIR

S07308.

RefSeq

NP_415209.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FU4	X-ray	1.80	A/B	1-83	[»]

ProteinModelPortal

P0A9A9.

SMR

P0A9A9. Positions 3-83.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-31858N.

IntAct

P0A9A9. 50 interactions.

MINT

MINT-1312814.

2D gel databases

SWISS-2DPAGE

P0A9A9.

ECO2DBASE

G015.8. 6TH EDITION.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000002403; EBESCP00000002403; EBESCG00000001959.
EBESCT00000002404; EBESCP00000002404; EBESCG00000001959.
EBESCT00000002405; EBESCP00000002405; EBESCG00000001959.
EBESCT00000002406; EBESCP00000002406; EBESCG00000001959.
EBESCT00000015746; EBESCP00000015037; EBESCG00000014806.

GeneID

945295.

GenomeReviews

Gene locus JW0669 in contig AP009048_GR.
Gene locus b0683 in contig U00096_GR.

KEGG

ecj:JW0669.
eco:b0683.

PATRIC

32116559. VBIEscCol129921_0712.

Organism-specific databases

EchoBASE

EB0354.

EcoGene

EG10359. fur.

Phylogenomic databases

eggNOG

COG0735.

GeneTree

EBGT00050000011411.

HOGENOM

HBG731501.

OMA

QFDEAHI.

PhylomeDB

P0A9A9.

ProtClustDB

PRK09462.

Enzyme and pathway databases

BioCyc

EcoCyc:PD00260.

Gene expression databases

Genevestigator

P0A9A9.

Family and domain databases

InterPro

IPR002481. FUR.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]

Gene3D

G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

K03711.

Pfam

PF01475. FUR. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FUR_ECOLI

Accession

Primary (citable) accession number: P0A9A9
Secondary accession number(s): P06975

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents