Reviewed, UniProtKB/Swiss-Prot P0A9A9 (FUR_ECOLI)

Names and origin

Protein names

Recommended name:
    Ferric uptake regulation protein
      Short name=Ferric uptake regulator

Gene names

Name:	fur
Ordered Locus Names:	b0683, JW0669

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a global negative controlling element, employing Fe2+ as a cofactor to bind the operator of the repressed genes. Regulates the expression of several outer-membrane proteins including the iron transport operon. Ref.5
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	Activated by cadmium, cobalt, copper, and manganese ions, but not zinc ions.
Sequence similarities	Belongs to the Fur family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	DNA-binding Iron Metal-binding Zinc
Molecular function	Repressor
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct transcription factor activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nadE	P18843	1	EBI-369487,EBI-548960

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 148

148

Ferric uptake regulation protein

PRO_0000095550

Regions

Region

1 – 84

84

DNA-binding By similarity

Region

85 – 148

64

Dimerization By similarity

Compositional bias

86 – 90

5

His-rich

Sites

Metal binding

33

1

Zinc By similarity

Metal binding

81

1

Zinc By similarity

Metal binding

87

1

Iron By similarity

Metal binding

89

1

Iron By similarity

Metal binding

90

1

Zinc By similarity

Metal binding

93

1

Zinc

Metal binding

96

1

Zinc

Metal binding

101

1

Zinc By similarity

Metal binding

108

1

Iron By similarity

Metal binding

125

1

Iron By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

148

Helix Strand Turn

Details...

Helix

4 – 10

7

Helix

17 – 26

10

Helix

29 – 31

3

Beta strand

32 – 34

3

Helix

36 – 45

10

Helix

52 – 64

13

Beta strand

67 – 72

6

Helix

74 – 76

3

Beta strand

78 – 82

5

Sequences

	Sequence		Length	Mass (Da)
	P0A9A9-1 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: B868360513489DD3	FASTA	148	16,795
	10 20 30 40 50 60 MTDNNTALKK AGLKVTLPRL KILEVLQEPD NHHVSAEDLY KRLIDMGEEI GLATVYRVLN 70 80 90 100 110 120 QFDDAGIVTR HNFEGGKSVF ELTQQHHHDH LICLDCGKVI EFSDDSIEAR QREIAAKHGI 130 140 RLTNHSLYLY GHCAEGDCRE DEHAHEGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the iron regulatory gene fur." Schaeffer S., Hantke K., Braun V. Mol. Gen. Genet. 200:110-113(1985) [PubMed: 2993806] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Ferric uptake regulation protein acts as a repressor, employing iron (II) as a cofactor to bind the operator of an iron transport operon in Escherichia coli." Bagg A., Neilands J.B. Biochemistry 26:5471-5477(1987) [PubMed: 2823881] [Abstract] Cited for: FUNCTION.
[6]	"Structural dynamics and functional domains of the fur protein." Coy M., Neilands J.B. Biochemistry 30:8201-8210(1991) [PubMed: 1868094] [Abstract] Cited for: STRUCTURAL DYNAMICS.
[7]	"Identification of the two zinc-bound cysteines in the ferric uptake regulation protein from Escherichia coli: chemical modification and mass spectrometry analysis." Gonzalez de Peredo A., Saint-Pierre C., Adrait A., Jacquamet L., Latour J.M., Michaud-Soret I., Forest E. Biochemistry 38:8582-8589(1999) [PubMed: 10387106] [Abstract] Cited for: ZINC-BINDING SITES.
[8]	"The binding of the ferric uptake regulation protein to a DNA fragment." Saito T., Williams R.J. Eur. J. Biochem. 197:43-47(1991) [PubMed: 2015825] [Abstract] Cited for: STRUCTURE BY NMR.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X02589 Genomic DNA. Translation: CAA26429.1.
U00096 Genomic DNA. Translation: AAC73777.1.
AP009048 Genomic DNA. Translation: BAA35331.1.

PIR

S07308.

RefSeq

AP_001321.1.
NP_415209.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2FU4	X-ray	1.80	A/B	1-83	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A9A9. 50 interactions.

2-D gel databases

SWISS-2DPAGE

P0A9A9.

ECO2DBASE

G015.8. 6TH EDITION.

Genome annotation databases

GeneID

945295.

GenomeReviews

Gene locus JW0669 in contig AP009048_GR.
Gene locus b0683 in contig U00096_GR.

KEGG

ecj:JW0669.
eco:b0683.

Organism-specific databases

EchoBASE

EB0354.

EcoGene

EG10359. fur.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A9A9.

OMA

P0A9A9. GKHHDHL.

Enzyme and pathway databases

BioCyc

EcoCyc:PD00260.

Family and domain databases

InterPro

IPR002481. FUR.
[Graphical view]

Pfam

PF01475. FUR. 1 hit.
[Graphical view]

ProDom

PD002003. FUR. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Entry information

Entry name

FUR_ECOLI

Accession

Primary (citable) accession number: P0A9A9
Secondary accession number(s): P06975

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)