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Protein

Anaerobic glycerol-3-phosphate dehydrogenase subunit C

Gene

glpC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Electron transfer protein; may also function as the membrane anchor for the GlpAB dimer.

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route).
Proteins known to be involved in this subpathway in this organism are:
  1. Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB), Anaerobic glycerol-3-phosphate dehydrogenase subunit A (glpA), Anaerobic glycerol-3-phosphate dehydrogenase subunit C (glpC)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route), the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi12 – 121Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi15 – 151Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi19 – 191Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi56 – 561Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi59 – 591Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi62 – 621Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  • iron-sulfur cluster binding Source: EcoCyc
  • metal ion binding Source: EcoCyc

GO - Biological processi

  • anaerobic respiration Source: EcoCyc
  • electron transport chain Source: EcoCyc
  • glycerol catabolic process Source: UniProtKB-UniPathway
  • transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:ANGLYC3PDEHYDROGSUBUNITC-MONOMER.
ECOL316407:JW2237-MONOMER.
MetaCyc:ANGLYC3PDEHYDROGSUBUNITC-MONOMER.
UniPathwayiUPA00618; UER00673.

Names & Taxonomyi

Protein namesi
Recommended name:
Anaerobic glycerol-3-phosphate dehydrogenase subunit C
Short name:
G-3-P dehydrogenase
Gene namesi
Name:glpC
Ordered Locus Names:b2243, JW2237
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10393. glpC.

Subcellular locationi

GO - Cellular componenti

  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396Anaerobic glycerol-3-phosphate dehydrogenase subunit CPRO_0000159259Add
BLAST

Proteomic databases

EPDiP0A996.
PaxDbiP0A996.
PRIDEiP0A996.

Interactioni

Subunit structurei

Composed of a catalytic GlpA/B dimer and of GlpC.

Protein-protein interaction databases

BioGridi4262942. 9 interactions.
DIPiDIP-35835N.
IntActiP0A996. 20 interactions.
STRINGi511145.b2243.

Structurei

3D structure databases

ProteinModelPortaliP0A996.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 29284Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini45 – 76324Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105CHG. Bacteria.
COG0247. LUCA.
HOGENOMiHOG000278500.
InParanoidiP0A996.
KOiK00113.
OMAiKEMPDNC.
OrthoDBiEOG6W45NM.
PhylomeDBiP0A996.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR004017. Cys_rich_dom.
IPR017753. G3P_DH_GlpC_su.
IPR009051. Helical_ferredxn.
[Graphical view]
PfamiPF02754. CCG. 2 hits.
PF13183. Fer4_8. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
TIGRFAMsiTIGR03379. glycerol3P_GlpC. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A996-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDTSFENCI KCTVCTTACP VSRVNPGYPG PKQAGPDGER LRLKDGALYD
60 70 80 90 100
EALKYCINCK RCEVACPSDV KIGDIIQRAR AKYDTTRPSL RNFVLSHTDL
110 120 130 140 150
MGSVSTPFAP IVNTATSLKP VRQLLDAALK IDHRRTLPKY SFGTFRRWYR
160 170 180 190 200
SVAAQQAQYK DQVAFFHGCF VNYNHPQLGK DLIKVLNAMG TGVQLLSKEK
210 220 230 240 250
CCGVPLIANG FTDKARKQAI TNVESIREAV GVKGIPVIAT SSTCTFALRD
260 270 280 290 300
EYPEVLNVDN KGLRDHIELA TRWLWRKLDE GKTLPLKPLP LKVVYHTPCH
310 320 330 340 350
MEKMGWTLYT LELLRNIPGL ELTVLDSQCC GIAGTYGFKK ENYPTSQAIG
360 370 380 390
APLFRQIEES GADLVVTDCE TCKWQIEMST SLRCEHPITL LAQALA
Length:396
Mass (Da):44,108
Last modified:July 19, 2005 - v1
Checksum:i1DC9203332B6F2F2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti169 – 1691C → V in AAA83866 (PubMed:3286606).Curated
Sequence conflicti206 – 2061L → V in AAA83866 (PubMed:3286606).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20938 Genomic DNA. Translation: AAA83866.1.
U00096 Genomic DNA. Translation: AAC75303.1.
AP009048 Genomic DNA. Translation: BAA16062.1.
PIRiA64995. DEECNC.
RefSeqiNP_416746.1. NC_000913.3.
WP_001000379.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75303; AAC75303; b2243.
BAA16062; BAA16062; BAA16062.
GeneIDi946735.
KEGGiecj:JW2237.
eco:b2243.
PATRICi32119845. VBIEscCol129921_2332.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20938 Genomic DNA. Translation: AAA83866.1.
U00096 Genomic DNA. Translation: AAC75303.1.
AP009048 Genomic DNA. Translation: BAA16062.1.
PIRiA64995. DEECNC.
RefSeqiNP_416746.1. NC_000913.3.
WP_001000379.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A996.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262942. 9 interactions.
DIPiDIP-35835N.
IntActiP0A996. 20 interactions.
STRINGi511145.b2243.

Proteomic databases

EPDiP0A996.
PaxDbiP0A996.
PRIDEiP0A996.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75303; AAC75303; b2243.
BAA16062; BAA16062; BAA16062.
GeneIDi946735.
KEGGiecj:JW2237.
eco:b2243.
PATRICi32119845. VBIEscCol129921_2332.

Organism-specific databases

EchoBASEiEB0388.
EcoGeneiEG10393. glpC.

Phylogenomic databases

eggNOGiENOG4105CHG. Bacteria.
COG0247. LUCA.
HOGENOMiHOG000278500.
InParanoidiP0A996.
KOiK00113.
OMAiKEMPDNC.
OrthoDBiEOG6W45NM.
PhylomeDBiP0A996.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00673.
BioCyciEcoCyc:ANGLYC3PDEHYDROGSUBUNITC-MONOMER.
ECOL316407:JW2237-MONOMER.
MetaCyc:ANGLYC3PDEHYDROGSUBUNITC-MONOMER.

Miscellaneous databases

PROiP0A996.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR004017. Cys_rich_dom.
IPR017753. G3P_DH_GlpC_su.
IPR009051. Helical_ferredxn.
[Graphical view]
PfamiPF02754. CCG. 2 hits.
PF13183. Fer4_8. 1 hit.
[Graphical view]
SUPFAMiSSF46548. SSF46548. 1 hit.
TIGRFAMsiTIGR03379. glycerol3P_GlpC. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and gene-polypeptide relationships of the glpABC operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12."
    Cole S.T., Eiglmeier K., Ahmed S., Honore N., Elmes L., Anderson W.F., Weiner J.H.
    J. Bacteriol. 170:2448-2456(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiGLPC_ECOLI
AccessioniPrimary (citable) accession number: P0A996
Secondary accession number(s): P13034, P76927, P77679
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: March 16, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.