P0A992 (ALF1_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-bisphosphate aldolase class 1
EC=4.1.2.13

Alternative name(s):
Fructose-bisphosphate aldolase class I
Short name=FBP aldolase

Gene names

Name:	fbaB
Ordered Locus Names:	c2623

Organism

Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

199310 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Subunit structure	Homooctamer or homodecamer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DeoC/FbaB aldolase family. FbaB subfamily.
Sequence caution	The sequence AAN81079.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 350	349	Fructose-bisphosphate aldolase class 1		PRO_0000138940
Sites
	Active site	237	1	Schiff-base intermediate with dihydroxyacetone-P
Amino acid modifications
	Modified residue	208	1	N6-acetyllysine By similarity
	Modified residue	262	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A992 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 462341BE2459E587	FASTA	350	38,109
	10 20 30 40 50 60 MTDIAQLLGK DADNLLQHRC MTIPSDQLYL PGHDYVDRVM IDNNRPPAVL RNMQTLYNTG 70 80 90 100 110 120 RLAGTGYLSI LPVDQGVEHS AGASFAANPL YFDPKNIVEL AIEAGCNCVA STYGVLASVS 130 140 150 160 170 180 RRYAHRIPFL VKLNHNETLS YPNTYDQTLY ASVEQAFNMG AVAVGATIYF GSEESRRQIE 190 200 210 220 230 240 EISAAFERAH ELGMVTVLWA YLRNSAFKKD GVDYHVSADL TGQANHLAAT IGADIVKQKM 250 260 270 280 290 300 AENNGGYKAI NYGYTDDRVY SKLTSENPID LVRYQLANCY MGRAGLINSG GAAGGETDLS 310 320 330 340 350 DAVRTAVINK RAGGMGLILG RKAFKKSMAD GVKLINAVQD VYLDSKITIA

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014075 Genomic DNA. Translation: AAN81079.1. Different initiation.
RefSeq	NP_754511.2. NC_004431.1.
3D structure databases
ProteinModelPortal	P0A992.
SMR	P0A992. Positions 70-341.
ModBase	Search...
Protein-protein interaction databases
STRING	199310.c2623.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN81079; AAN81079; c2623.
GeneID	1037944.
KEGG	ecc:c2623.
PATRIC	18283118. VBIEscCol75197_2467.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000224791.
KO	K11645.
OMA	RYQVLNC.
ProtClustDB	PRK09250.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR002915. DeoC/FbaB/lacD_aldolase. [Graphical view]
Pfam	PF01791. DeoC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF1_ECOL6

Accession

Primary (citable) accession number: P0A992
Secondary accession number(s): P71295, P76416

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 54 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program