P0A991 (ALF1_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-bisphosphate aldolase class 1
EC=4.1.2.13

Alternative name(s):
Fructose-bisphosphate aldolase class I
Short name=FBP aldolase

Gene names

Name:	fbaB
Synonyms:	dhnA
Ordered Locus Names:	b2097, JW5344

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Enzyme regulation	Activated by citrate.
Subunit structure	Homooctamer or homodecamer.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DeoC/FbaB aldolase family. FbaB subfamily.
Caution	Was originally (Ref.1) thought to be a dehydrin.
Mass spectrometry	Molecular mass is 37979.3 Da from positions 2 - 350. Determined by MALDI. Ref.4
Sequence caution	The sequence AAB18249.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytosol Inferred from direct assay PubMed 16858726. Source: UniProtKB membrane Inferred from direct assay PubMed 16858726. Source: UniProtKB
Molecular_function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		2	EBI-545269,EBI-545269

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 350	349	Fructose-bisphosphate aldolase class 1		PRO_0000138939
Sites
	Active site	237	1	Schiff-base intermediate with dihydroxyacetone-P
Amino acid modifications
	Modified residue	208	1	N6-acetyllysine Ref.5
	Modified residue	262	1	N6-acetyllysine Ref.5
Experimental info
	Mutagenesis	237	1	K → A: Loss of activity.
	Mutagenesis	239	1	K → A: No change in activity.
	Sequence conflict	192	1	L → V in AAB18249. Ref.1
	Sequence conflict	309	1	N → I in AAB18249. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A991 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 462341BE2459E587	FASTA	350	38,109
	10 20 30 40 50 60 MTDIAQLLGK DADNLLQHRC MTIPSDQLYL PGHDYVDRVM IDNNRPPAVL RNMQTLYNTG 70 80 90 100 110 120 RLAGTGYLSI LPVDQGVEHS AGASFAANPL YFDPKNIVEL AIEAGCNCVA STYGVLASVS 130 140 150 160 170 180 RRYAHRIPFL VKLNHNETLS YPNTYDQTLY ASVEQAFNMG AVAVGATIYF GSEESRRQIE 190 200 210 220 230 240 EISAAFERAH ELGMVTVLWA YLRNSAFKKD GVDYHVSADL TGQANHLAAT IGADIVKQKM 250 260 270 280 290 300 AENNGGYKAI NYGYTDDRVY SKLTSENPID LVRYQLANCY MGRAGLINSG GAAGGETDLS 310 320 330 340 350 DAVRTAVINK RAGGMGLILG RKAFKKSMAD GVKLINAVQD VYLDSKITIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Close T.J., Choi D.W. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The dhnA gene of Escherichia coli encodes a class I fructose bisphosphate aldolase." Thomson G.J., Howlett G.J., Ashcroft A.E., Berry A. Biochem. J. 331:437-445(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-10, CHARACTERIZATION, MASS SPECTROMETRY.
[5]	"Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y. Mol. Cell. Proteomics 8:215-225(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-208 AND LYS-262, MASS SPECTROMETRY. Strain: K12 / JW1106 and K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U73760 Genomic DNA. Translation: AAB18249.1. Different initiation. U00096 Genomic DNA. Translation: AAC75158.2. AP009048 Genomic DNA. Translation: BAE76584.1.
RefSeq	NP_416600.4. NC_000913.2. YP_490337.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A991.
SMR	P0A991. Positions 70-341.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-36197N.
IntAct	P0A991. 5 interactions.
STRING	511145.b2097.
Proteomic databases
PaxDb	P0A991.
PRIDE	P0A991.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75158; AAC75158; b2097. BAE76584; BAE76584; BAE76584.
GeneID	12932531. 946632.
KEGG	ecj:Y75_p2059. eco:b2097.
PATRIC	32119527. VBIEscCol129921_2174.
Organism-specific databases
EchoBASE	EB3815.
EcoGene	EG14062. fbaB.
Phylogenomic databases
eggNOG	COG1830.
HOGENOM	HOG000224791.
KO	K11645.
OMA	RYQVLNC.
ProtClustDB	PRK09250.
Enzyme and pathway databases
BioCyc	EcoCyc:FRUCBISALD-CLASSI-MONOMER. ECOL316407:JW5344-MONOMER. MetaCyc:FRUCBISALD-CLASSI-MONOMER.
SABIO-RK	P0A991.
Gene expression databases
Genevestigator	P0A991.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR002915. DeoC/FbaB/lacD_aldolase. [Graphical view]
Pfam	PF01791. DeoC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF1_ECOLI

Accession

Primary (citable) accession number: P0A991
Secondary accession number(s): P71295, P76416, Q2MAX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program