P0A978 (CSPG_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cold shock-like protein CspG
Short name=CPS-G

Gene names

Name:	cspG
Synonyms:	cspI
Ordered Locus Names:	b0990, JW0974

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	70 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cytoplasm By similarity.
Induction	In response to low temperature.
Sequence similarities	Contains 1 CSD (cold-shock) domain.

Ontologies

Keywords
Biological process	Stress response Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
rplA	P0A7L0	2	EBI-547581,EBI-543771

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 70	70	Cold shock-like protein CspG		PRO_0000100262
Regions
	Domain	7 – 67	61	CSD

Sequences

	Sequence		Length	Mass (Da)
	P0A978 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 3B9910355A105D3C	FASTA	70	7,781
	10 20 30 40 50 60 MSNKMTGLVK WFNADKGFGF ITPDDGSKDV FVHFTAIQSN EFRTLNENQK VEFSIEQGQR 70 GPAAANVVTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel member of the cspA family of genes that is induced by cold shock in Escherichia coli." Nakashima K., Kanamaru K., Mizuno T., Horikoshi K. J. Bacteriol. 178:2994-2997(1996) [PubMed: 8631696] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Detection and speciation of bacteria through PCR using universal major cold-shock protein primer oligomers." Francis K.P., Stewart G.S.A.B. J. Ind. Microbiol. Biotechnol. 19:286-293(1997) [PubMed: 9439003] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D63344 mRNA. Translation: BAA09669.1. AF003591 Genomic DNA. Translation: AAB61741.1. U00096 Genomic DNA. Translation: AAC74075.1. AP009048 Genomic DNA. Translation: BAA35756.1.
PIR	D64840.
RefSeq	NP_415510.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P0A978.
SMR	P0A978. Positions 2-70.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-47938N.
IntAct	P0A978. 23 interactions.
MINT	MINT-1247768.
Proteomic databases
PRIDE	P0A978.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000003528; EBESCP00000003528; EBESCG00000002885. EBESCT00000016965; EBESCP00000016256; EBESCG00000016024.
GeneID	945591.
GenomeReviews	Gene locus JW0974 in contig AP009048_GR. Gene locus b0990 in contig U00096_GR.
KEGG	ecj:JW0974. eco:b0990.
PATRIC	32117205. VBIEscCol129921_1026.
Organism-specific databases
EchoBASE	EB3993.
EcoGene	EG14243. cspG.
Phylogenomic databases
eggNOG	COG1278.
GeneTree	EBGT00050000010462.
HOGENOM	HBG717773.
OMA	FITPQDG.
PhylomeDB	P0A978.
ProtClustDB	PRK09890.
Enzyme and pathway databases
BioCyc	EcoCyc:G6511-MONOMER.
Gene expression databases
Genevestigator	P0A978.
Family and domain databases
InterPro	IPR019844. Cold-shock_CS. IPR012156. Cold_shock_CspA. IPR011129. Cold_shock_prot. IPR002059. CSP_DNA-bd. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K03704.
Pfam	PF00313. CSD. 1 hit. [Graphical view]
PIRSF	PIRSF002599. Cold_shock_A. 1 hit.
PRINTS	PR00050. COLDSHOCK.
SMART	SM00357. CSP. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
PROSITE	PS00352. COLD_SHOCK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CSPG_ECOLI

Accession

Primary (citable) accession number: P0A978
Secondary accession number(s): Q47130

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program