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Protein

Cold shock-like protein CspE

Gene

cspE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • poly(A) binding Source: EcoCyc
  • RNA binding Source: EcoliWiki
  • single-stranded DNA binding Source: EcoCyc
  • transcription antitermination factor activity, RNA binding Source: EcoliWiki

GO - Biological processi

  • negative regulation of DNA-templated transcription, termination Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
  • transcription antitermination Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12179-MONOMER.
ECOL316407:JW0618-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cold shock-like protein CspE
Short name:
CSP-E
Gene namesi
Name:cspE
Synonyms:gicA, msmC
Ordered Locus Names:b0623, JW0618
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12179. cspE.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 6968Cold shock-like protein CspEPRO_0000100253Add
BLAST

Proteomic databases

PaxDbiP0A972.
PRIDEiP0A972.

Interactioni

Protein-protein interaction databases

BioGridi4260642. 12 interactions.
DIPiDIP-47834N.
IntActiP0A972. 48 interactions.
MINTiMINT-1221433.
STRINGi511145.b0623.

Structurei

3D structure databases

ProteinModelPortaliP0A972.
SMRiP0A972. Positions 1-69.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 6661CSDAdd
BLAST

Sequence similaritiesi

Contains 1 CSD (cold-shock) domain.Curated

Phylogenomic databases

eggNOGiCOG1278. LUCA.
HOGENOMiHOG000070674.
InParanoidiP0A972.
KOiK03704.
OMAiHSEIQES.
OrthoDBiEOG618R0J.
PhylomeDBiP0A972.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR019844. Cold-shock_CS.
IPR012156. Cold_shock_CspA.
IPR011129. Cold_shock_prot.
IPR002059. CSP_DNA-bd.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF00313. CSD. 1 hit.
[Graphical view]
PIRSFiPIRSF002599. Cold_shock_A. 1 hit.
PRINTSiPR00050. COLDSHOCK.
SMARTiSM00357. CSP. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiPS00352. COLD_SHOCK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A972-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKIKGNVKW FNESKGFGFI TPEDGSKDVF VHFSAIQTNG FKTLAEGQRV
60
EFEITNGAKG PSAANVIAL
Length:69
Mass (Da):7,463
Last modified:January 23, 2007 - v2
Checksum:i1C33C920AA82405C
GO

Sequence cautioni

The sequence AAB40823.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti58 – 581A → R (Ref. 2) Curated

Mass spectrometryi

Molecular mass is 7331 Da from positions 2 - 69. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28497 Genomic DNA. Translation: BAA05856.1.
L29054 Genomic DNA. Translation: AAA67556.1.
S83396 Genomic DNA. Translation: AAN86720.1.
U82598 Genomic DNA. Translation: AAB40823.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73724.1.
AP009048 Genomic DNA. Translation: BAA35266.1.
PIRiS49050.
RefSeqiNP_415156.1. NC_000913.3.
WP_000034825.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73724; AAC73724; b0623.
BAA35266; BAA35266; BAA35266.
GeneIDi23337261.
947024.
KEGGiecj:JW0618.
eco:b0623.
PATRICi32116426. VBIEscCol129921_0653.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D28497 Genomic DNA. Translation: BAA05856.1.
L29054 Genomic DNA. Translation: AAA67556.1.
S83396 Genomic DNA. Translation: AAN86720.1.
U82598 Genomic DNA. Translation: AAB40823.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73724.1.
AP009048 Genomic DNA. Translation: BAA35266.1.
PIRiS49050.
RefSeqiNP_415156.1. NC_000913.3.
WP_000034825.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A972.
SMRiP0A972. Positions 1-69.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260642. 12 interactions.
DIPiDIP-47834N.
IntActiP0A972. 48 interactions.
MINTiMINT-1221433.
STRINGi511145.b0623.

Proteomic databases

PaxDbiP0A972.
PRIDEiP0A972.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73724; AAC73724; b0623.
BAA35266; BAA35266; BAA35266.
GeneIDi23337261.
947024.
KEGGiecj:JW0618.
eco:b0623.
PATRICi32116426. VBIEscCol129921_0653.

Organism-specific databases

EchoBASEiEB2096.
EcoGeneiEG12179. cspE.

Phylogenomic databases

eggNOGiCOG1278. LUCA.
HOGENOMiHOG000070674.
InParanoidiP0A972.
KOiK03704.
OMAiHSEIQES.
OrthoDBiEOG618R0J.
PhylomeDBiP0A972.

Enzyme and pathway databases

BioCyciEcoCyc:EG12179-MONOMER.
ECOL316407:JW0618-MONOMER.

Miscellaneous databases

PROiP0A972.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
InterProiIPR019844. Cold-shock_CS.
IPR012156. Cold_shock_CspA.
IPR011129. Cold_shock_prot.
IPR002059. CSP_DNA-bd.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamiPF00313. CSD. 1 hit.
[Graphical view]
PIRSFiPIRSF002599. Cold_shock_A. 1 hit.
PRINTSiPR00050. COLDSHOCK.
SMARTiSM00357. CSP. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiPS00352. COLD_SHOCK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and characterization of multicopy suppressors of a mukB mutation in Escherichia coli."
    Yamanaka K., Mitani T., Ogura T., Niki H., Hiraga S.
    Mol. Microbiol. 13:301-312(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Ramanathan Y., Narayanarao A.S.S., Mathur M., Mahajan S.K.
    Submitted (FEB-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Overproduction of three genes leads to camphor resistance and chromosome condensation in Escherichia coli."
    Hu K.H., Liu E., Dean K., Gingras M., Degraff W., Trun N.J.
    Genetics 143:1521-1532(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  8. Liu K., Hanna M.M.
    Submitted (JUN-1995) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 2-46.
    Strain: AD7333.
  9. "The cold-shock response -- a hot topic."
    Jones P.G., Inouye M.
    Mol. Microbiol. 11:811-818(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME, GENE MAPPING.
  10. "Gas-phase concentration, purification, and identification of whole proteins from complex mixtures."
    Reid G.E., Shang H., Hogan J.M., Lee G.U., McLuckey S.A.
    J. Am. Chem. Soc. 124:7353-7362(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: MASS SPECTROMETRY.
    Strain: ATCC 15597.

Entry informationi

Entry nameiCSPE_ECOLI
AccessioniPrimary (citable) accession number: P0A972
Secondary accession number(s): P36997, P77103, P80434
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: January 23, 2007
Last modified: January 20, 2016
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.