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Protein

Cold shock-like protein CspD

Gene

cspD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibits DNA replication at both initiation and elongation steps, most probably by binding to the opened, single-stranded regions at replication forks. Plays a regulatory role in chromosomal replication in nutrient-depleted cells.
Involved in persister cell formation, acting downstream of mRNA interferase (toxin) MqsR. Overproduction is toxic.

Miscellaneous

Binds single-stranded DNA and RNA, but not double-stranded DNA, through hydrophobic interactions without sequence specificity, resulting in a packed structure.

GO - Molecular functioni

  • RNA binding Source: EcoCyc
  • single-stranded DNA binding Source: EcoCyc
  • toxin activity Source: UniProtKB-KW

GO - Biological processi

  • negative regulation of DNA replication Source: EcoCyc
  • regulation of transcription, DNA-templated Source: InterPro
  • response to starvation Source: EcoliWiki

Keywordsi

Molecular functionDNA replication inhibitor, DNA-binding, RNA-binding, Toxin

Enzyme and pathway databases

BioCyciEcoCyc:EG11111-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Cold shock-like protein CspD
Short name:
CSP-D
Gene namesi
Name:cspD
Synonyms:cspH, ybjA
Ordered Locus Names:b0880, JW0864
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11111 cspD

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Deletion represses the production of persister cells.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001002481 – 74Cold shock-like protein CspDAdd BLAST74

Proteomic databases

EPDiP0A968
PaxDbiP0A968
PRIDEiP0A968

Expressioni

Inductioni

Not induced by cold-shock. Stationary-phase and starvation inducible, as well as by oxidative stress (30 mM H2O2). Repressed by MqsA and MqsRA toxin-antitoxin system.1 Publication

Interactioni

Subunit structurei

Homodimer.

Binary interactionsi

WithEntry#Exp.IntActNotes
dppDP0AAG03EBI-547937,EBI-548206

Protein-protein interaction databases

BioGridi4263137, 624 interactors
DIPiDIP-47833N
IntActiP0A968, 39 interactors
STRINGi316385.ECDH10B_0950

Structurei

3D structure databases

ProteinModelPortaliP0A968
SMRiP0A968
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 64CSDAdd BLAST61

Phylogenomic databases

eggNOGiENOG4105VEQ Bacteria
COG1278 LUCA
HOGENOMiHOG000070674
InParanoidiP0A968
KOiK03704
OMAiHYSTIKM
PhylomeDBiP0A968

Family and domain databases

CDDicd04458 CSP_CDS, 1 hit
InterProiView protein in InterPro
IPR019844 Cold-shock_CS
IPR012156 Cold_shock_CspA
IPR011129 CSD
IPR002059 CSP_DNA-bd
IPR012751 CspD
IPR012340 NA-bd_OB-fold
PfamiView protein in Pfam
PF00313 CSD, 1 hit
PIRSFiPIRSF002599 Cold_shock_A, 1 hit
PRINTSiPR00050 COLDSHOCK
SMARTiView protein in SMART
SM00357 CSP, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR02381 cspD, 1 hit
PROSITEiView protein in PROSITE
PS00352 CSD_1, 1 hit
PS51857 CSD_2, 1 hit

Sequencei

Sequence statusi: Complete.

P0A968-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKGTVKWFN NAKGFGFICP EGGGEDIFAH YSTIQMDGYR TLKAGQSVQF
60 70
DVHQGPKGNH ASVIVPVEVE AAVA
Length:74
Mass (Da):7,969
Last modified:July 19, 2005 - v1
Checksum:iC74AB028135BC22C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti19C → E AA sequence (PubMed:9868784).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31045 Genomic DNA No translation available.
U00096 Genomic DNA Translation: AAC73967.1
AP009048 Genomic DNA Translation: BAA35599.1
PIRiH64826
RefSeqiNP_415401.1, NC_000913.3
WP_000410785.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73967; AAC73967; b0880
BAA35599; BAA35599; BAA35599
GeneIDi945669
KEGGiecj:JW0864
eco:b0880
PATRICifig|1411691.4.peg.1397

Similar proteinsi

Entry informationi

Entry nameiCSPD_ECOLI
AccessioniPrimary (citable) accession number: P0A968
Secondary accession number(s): P24245
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health