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P0A968 (CSPD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cold shock-like protein CspD

Short name=CSP-D
Gene names
Name:cspD
Synonyms:cspH, ybjA
Ordered Locus Names:b0880, JW0864
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length74 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits DNA replication at both initiation and elongation steps, most probably by binding to the opened, single-stranded regions at replication forks. Plays a regulatory role in chromosomal replication in nutrient-depleted cells. Ref.8 Ref.9

Involved in persister cell formation, acting downstream of mRNA interferase (toxin) MqsR. Overproduction is toxic. Ref.8 Ref.9

Subunit structure

Homodimer.

Subcellular location

Cytoplasm.

Induction

Not induced by cold-shock. Stationary-phase and starvation inducible, as well as by oxidative stress (30 mM H2O2). Repressed by MqsA and MqsRA toxin-antitoxin system. Ref.9

Disruption phenotype

Deletion represses the production of persister cells. Ref.8

Miscellaneous

Binds single-stranded DNA and RNA, but not double-stranded DNA, through hydrophobic interactions without sequence specificity, resulting in a packed structure.

Sequence similarities

Contains 1 CSD (cold-shock) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7474Cold shock-like protein CspD
PRO_0000100248

Regions

Domain4 – 6461CSD

Experimental info

Sequence conflict191C → E AA sequence Ref.5

Sequences

Sequence LengthMass (Da)Tools
P0A968 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: C74AB028135BC22C

FASTA747,969
        10         20         30         40         50         60 
MEKGTVKWFN NAKGFGFICP EGGGEDIFAH YSTIQMDGYR TLKAGQSVQF DVHQGPKGNH 

        70 
ASVIVPVEVE AAVA 

« Hide

References

« Hide 'large scale' references
[1]"The ATP-dependent Clp protease of Escherichia coli. Sequence of clpA and identification of a Clp-specific substrate."
Gottesman S., Clark W.P., Maurizi M.R.
J. Biol. Chem. 265:7886-7893(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Small genes/gene-products in Escherichia coli K-12."
Wasinger V.C., Humphery-Smith I.
FEMS Microbiol. Lett. 169:375-382(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-20.
Strain: K12.
[6]"The product of unr, the highly conserved gene upstream of N-ras, contains multiple repeats similar to the cold-shock domain (CSD), a putative DNA-binding motif."
Doniger J., Landsman D., Gonda M.A., Wistow G.
New Biol. 4:389-395(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: SIMILARITY TO OTHER CSD PROTEINS.
[7]"CspD, a novel DNA replication inhibitor induced during the stationary phase in Escherichia coli."
Yamanaka K., Zheng W., Crooke E., Wang Y.-H., Inouye M.
Mol. Microbiol. 39:1572-1584(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]"Toxins Hha and CspD and small RNA regulator Hfq are involved in persister cell formation through MqsR in Escherichia coli."
Kim Y., Wood T.K.
Biochem. Biophys. Res. Commun. 391:209-213(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN PERSISTER CELL FORMATION, DISRUPTION PHENOTYPE.
Strain: K12 / BW25113.
[9]"Escherichia coli toxin/antitoxin pair MqsR/MqsA regulate toxin CspD."
Kim Y., Wang X., Zhang X.S., Grigoriu S., Page R., Peti W., Wood T.K.
Environ. Microbiol. 12:1105-1121(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION AS A TOXIN, INDUCTION.
Strain: K12 / BW25113.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M31045 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC73967.1.
AP009048 Genomic DNA. Translation: BAA35599.1.
PIRH64826.
RefSeqNP_415401.1. NC_000913.3.
YP_489153.1. NC_007779.1.

3D structure databases

ProteinModelPortalP0A968.
SMRP0A968. Positions 1-64.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-47833N.
IntActP0A968. 39 interactions.
MINTMINT-1219798.
STRING511145.b0880.

Proteomic databases

PaxDbP0A968.
PRIDEP0A968.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC73967; AAC73967; b0880.
BAA35599; BAA35599; BAA35599.
GeneID12934143.
945669.
KEGGecj:Y75_p0853.
eco:b0880.
PATRIC32116969. VBIEscCol129921_0910.

Organism-specific databases

EchoBASEEB1102.
EcoGeneEG11111. cspD.

Phylogenomic databases

eggNOGCOG1278.
HOGENOMHOG000070674.
KOK03704.
OMAQQVQFDV.
OrthoDBEOG618R0J.
PhylomeDBP0A968.

Enzyme and pathway databases

BioCycEcoCyc:EG11111-MONOMER.
ECOL316407:JW0864-MONOMER.

Gene expression databases

GenevestigatorP0A968.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
InterProIPR019844. Cold-shock_CS.
IPR012156. Cold_shock_CspA.
IPR011129. Cold_shock_prot.
IPR002059. CSP_DNA-bd.
IPR012751. CspD.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF00313. CSD. 1 hit.
[Graphical view]
PIRSFPIRSF002599. Cold_shock_A. 1 hit.
PRINTSPR00050. COLDSHOCK.
SMARTSM00357. CSP. 1 hit.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR02381. cspD. 1 hit.
PROSITEPS00352. COLD_SHOCK. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP0A968.

Entry information

Entry nameCSPD_ECOLI
AccessionPrimary (citable) accession number: P0A968
Secondary accession number(s): P24245
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: May 14, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene