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P0A964

- CHEW_ECOLI

UniProt

P0A964 - CHEW_ECOLI

Protein

Chemotaxis protein CheW

Gene

cheW

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Apr 1988)
      Previous versions | rss
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    Functioni

    Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. It physically bridges CheA to the MCPs (methyl-accepting chemotaxis proteins) to allow regulated phosphotransfer to CheY and CheB.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. signal transducer activity Source: InterPro

    GO - Biological processi

    1. chemotaxis Source: EcoCyc
    2. establishment of localization in cell Source: CACAO
    3. signal transduction Source: EcoCyc

    Keywords - Biological processi

    Chemotaxis

    Enzyme and pathway databases

    BioCyciEcoCyc:CHEW-MONOMER.
    ECOL316407:JW1876-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chemotaxis protein CheW
    Gene namesi
    Name:cheW
    Ordered Locus Names:b1887, JW1876
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10149. cheW.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: EcoCyc

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 167167Chemotaxis protein CheWPRO_0000198341Add
    BLAST

    Proteomic databases

    PaxDbiP0A964.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A964.

    Interactioni

    Subunit structurei

    An in vitro complex of CheW/CheA(L)/CheA(S) in a 1:1:1 ratio increases the autophosphorylation of CheA and is required for the binding of CheY, the phosphorylation substrate. This complex accounts for 10% of the total number of molecules.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    tarP070173EBI-1125947,EBI-1125130

    Protein-protein interaction databases

    DIPiDIP-48236N.
    IntActiP0A964. 31 interactions.
    MINTiMINT-1283783.
    STRINGi511145.b1887.

    Structurei

    Secondary structure

    1
    167
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi10 – 134
    Beta strandi17 – 248
    Beta strandi27 – 326
    Helixi33 – 353
    Beta strandi36 – 427
    Beta strandi55 – 617
    Beta strandi64 – 696
    Helixi71 – 755
    Beta strandi87 – 937
    Beta strandi96 – 11116
    Turni113 – 1153
    Beta strandi122 – 1254
    Helixi128 – 1303
    Beta strandi133 – 1375
    Beta strandi140 – 1445
    Helixi147 – 15812
    Helixi159 – 1613

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2HO9NMR-A1-167[»]
    ProteinModelPortaliP0A964.
    SMRiP0A964. Positions 1-167.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0A964.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini16 – 156141CheW-likePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 cheW-like domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0835.
    HOGENOMiHOG000254712.
    KOiK03408.
    OMAiVTYNDNT.
    OrthoDBiEOG60CWQ7.
    PhylomeDBiP0A964.

    Family and domain databases

    InterProiIPR002545. CheW.
    [Graphical view]
    PfamiPF01584. CheW. 1 hit.
    [Graphical view]
    SMARTiSM00260. CheW. 1 hit.
    [Graphical view]
    SUPFAMiSSF50341. SSF50341. 1 hit.
    PROSITEiPS50851. CHEW. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A964-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTGMTNVTKL ASEPSGQEFL VFTLGDEEYG IDILKVQEIR GYDQVTRIAN    50
    TPAFIKGVTN LRGVIVPIVD LRIKFSQVDV DYNDNTVVIV LNLGQRVVGI 100
    VVDGVSDVLS LTAEQIRPAP EFAVTLSTEY LTGLGALGDR MLILVNIEKL 150
    LNSEEMALLD SAASEVA 167
    Length:167
    Mass (Da):18,084
    Last modified:April 1, 1988 - v1
    Checksum:i9CE642E5F4E91BFF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 161G → R in AAA23575. (PubMed:2002011)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13462 Genomic DNA. Translation: AAA23565.1.
    U00096 Genomic DNA. Translation: AAC74957.1.
    AP009048 Genomic DNA. Translation: BAA15703.1.
    M34669 Genomic DNA. Translation: AAA23575.1.
    PIRiB25195. QRECCW.
    RefSeqiNP_416401.1. NC_000913.3.
    YP_490149.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74957; AAC74957; b1887.
    BAA15703; BAA15703; BAA15703.
    GeneIDi12932044.
    946400.
    KEGGiecj:Y75_p1863.
    eco:b1887.
    PATRICi32119101. VBIEscCol129921_1968.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M13462 Genomic DNA. Translation: AAA23565.1 .
    U00096 Genomic DNA. Translation: AAC74957.1 .
    AP009048 Genomic DNA. Translation: BAA15703.1 .
    M34669 Genomic DNA. Translation: AAA23575.1 .
    PIRi B25195. QRECCW.
    RefSeqi NP_416401.1. NC_000913.3.
    YP_490149.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2HO9 NMR - A 1-167 [» ]
    ProteinModelPortali P0A964.
    SMRi P0A964. Positions 1-167.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48236N.
    IntActi P0A964. 31 interactions.
    MINTi MINT-1283783.
    STRINGi 511145.b1887.

    Proteomic databases

    PaxDbi P0A964.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74957 ; AAC74957 ; b1887 .
    BAA15703 ; BAA15703 ; BAA15703 .
    GeneIDi 12932044.
    946400.
    KEGGi ecj:Y75_p1863.
    eco:b1887.
    PATRICi 32119101. VBIEscCol129921_1968.

    Organism-specific databases

    EchoBASEi EB0147.
    EcoGenei EG10149. cheW.

    Phylogenomic databases

    eggNOGi COG0835.
    HOGENOMi HOG000254712.
    KOi K03408.
    OMAi VTYNDNT.
    OrthoDBi EOG60CWQ7.
    PhylomeDBi P0A964.

    Enzyme and pathway databases

    BioCyci EcoCyc:CHEW-MONOMER.
    ECOL316407:JW1876-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P0A964.
    PROi P0A964.

    Gene expression databases

    Genevestigatori P0A964.

    Family and domain databases

    InterProi IPR002545. CheW.
    [Graphical view ]
    Pfami PF01584. CheW. 1 hit.
    [Graphical view ]
    SMARTi SM00260. CheW. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50341. SSF50341. 1 hit.
    PROSITEi PS50851. CHEW. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli."
      Mutoh N., Simon M.I.
      J. Bacteriol. 165:161-166(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Tandem translation starts in the cheA locus of Escherichia coli."
      Kofoid E.C., Parkinson J.S.
      J. Bacteriol. 173:2116-2119(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
    6. "Bacterial chemotaxis signaling complexes: formation of a CheA/CheW complex enhances autophosphorylation and affinity for CheY."
      McNally D.F., Matsumura P.
      Proc. Natl. Acad. Sci. U.S.A. 88:6269-6273(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT.

    Entry informationi

    Entry nameiCHEW_ECOLI
    AccessioniPrimary (citable) accession number: P0A964
    Secondary accession number(s): P07365
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1988
    Last sequence update: April 1, 1988
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3