P0A964 (CHEW_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chemotaxis protein CheW

Gene names

Name:	cheW
Ordered Locus Names:	b1887, JW1876

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. It physically bridges CheA to the MCPs (methyl-accepting chemotaxis proteins) to allow regulated phosphotransfer to CheY and CheB.
Subunit structure	An in vitro complex of CheW/CheA(L)/CheA(S) in a 1:1:1 ratio increases the autophosphorylation of CheA and is required for the binding of CheY, the phosphorylation substrate. This complex accounts for 10% of the total number of molecules. Ref.6
Subcellular location	Cytoplasm.
Sequence similarities	Contains 1 cheW-like domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chemotaxis Inferred from mutant phenotype PubMed 2682657. Source: EcoCyc signal transduction Inferred from direct assay PubMed 1992467. Source: EcoCyc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
tar	P07017	3	EBI-1125947,EBI-1125130

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 167

167

Chemotaxis protein CheW

PRO_0000198341

Regions

Domain

16 – 156

141

CheW-like

Experimental info

Sequence conflict

16

1

G → R in AAA23575. Ref.5

Secondary structure

1

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167

Helix Strand Turn

Details...

Beta strand

10 – 13

4

Beta strand

17 – 24

8

Beta strand

27 – 32

6

Helix

33 – 35

3

Beta strand

36 – 42

7

Beta strand

55 – 61

7

Beta strand

64 – 69

6

Helix

71 – 75

5

Beta strand

87 – 93

7

Beta strand

96 – 111

16

Turn

113 – 115

3

Beta strand

122 – 125

4

Helix

128 – 130

3

Beta strand

133 – 137

5

Beta strand

140 – 144

5

Helix

147 – 158

12

Helix

159 – 161

3

Sequences

	Sequence		Length	Mass (Da)
	P0A964 [UniParc]. Last modified April 1, 1988. Version 1. Checksum: 9CE642E5F4E91BFF	FASTA	167	18,084
	10 20 30 40 50 60 MTGMTNVTKL ASEPSGQEFL VFTLGDEEYG IDILKVQEIR GYDQVTRIAN TPAFIKGVTN 70 80 90 100 110 120 LRGVIVPIVD LRIKFSQVDV DYNDNTVVIV LNLGQRVVGI VVDGVSDVLS LTAEQIRPAP 130 140 150 160 EFAVTLSTEY LTGLGALGDR MLILVNIEKL LNSEEMALLD SAASEVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli." Mutoh N., Simon M.I. J. Bacteriol. 165:161-166(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Tandem translation starts in the cheA locus of Escherichia coli." Kofoid E.C., Parkinson J.S. J. Bacteriol. 173:2116-2119(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
[6]	"Bacterial chemotaxis signaling complexes: formation of a CheA/CheW complex enhances autophosphorylation and affinity for CheY." McNally D.F., Matsumura P. Proc. Natl. Acad. Sci. U.S.A. 88:6269-6273(1991) [PubMed] [Europe PMC] [Abstract] Cited for: SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M13462 Genomic DNA. Translation: AAA23565.1.
U00096 Genomic DNA. Translation: AAC74957.1.
AP009048 Genomic DNA. Translation: BAA15703.1.
M34669 Genomic DNA. Translation: AAA23575.1.

PIR

QRECCW. B25195.

RefSeq

NP_416401.1. NC_000913.2.
YP_490149.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HO9	NMR	-	A	1-167	[»]

ProteinModelPortal

P0A964.

SMR

P0A964. Positions 1-167.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48236N.

IntAct

P0A964. 28 interactions.

MINT

MINT-1283783.

STRING

511145.b1887.

Proteomic databases

PaxDb

P0A964.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC74957; AAC74957; b1887.
BAA15703; BAA15703; BAA15703.

GeneID

12932044.
946400.

KEGG

ecj:Y75_p1863.
eco:b1887.

PATRIC

32119101. VBIEscCol129921_1968.

Organism-specific databases

EchoBASE

EB0147.

EcoGene

EG10149. cheW.

Phylogenomic databases

eggNOG

COG0835.

HOGENOM

HOG000254712.

KO

K03408.

OMA

NSDEMAL.

ProtClustDB

PRK10612.

Enzyme and pathway databases

BioCyc

EcoCyc:CHEW-MONOMER.
ECOL316407:JW1876-MONOMER.

Gene expression databases

Genevestigator

P0A964.

Family and domain databases

InterPro

IPR002545. CheW.
[Graphical view]

Pfam

PF01584. CheW. 1 hit.
[Graphical view]

SMART

SM00260. CheW. 1 hit.
[Graphical view]

SUPFAM

SSF50341. CheW. 1 hit.

PROSITE

PS50851. CHEW. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0A964.

Entry information

Entry name

CHEW_ECOLI

Accession

Primary (citable) accession number: P0A964
Secondary accession number(s): P07365

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1988
Last sequence update:	April 1, 1988
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program