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P0A964 (CHEW_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chemotaxis protein CheW
Gene names
Name:cheW
Ordered Locus Names:b1887, JW1876
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length167 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. It physically bridges CheA to the MCPs (methyl-accepting chemotaxis proteins) to allow regulated phosphotransfer to CheY and CheB.

Subunit structure

An in vitro complex of CheW/CheA(L)/CheA(S) in a 1:1:1 ratio increases the autophosphorylation of CheA and is required for the binding of CheY, the phosphorylation substrate. This complex accounts for 10% of the total number of molecules. Ref.6

Subcellular location

Cytoplasm.

Sequence similarities

Contains 1 cheW-like domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

tarP070173EBI-1125947,EBI-1125130

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 167167Chemotaxis protein CheW
PRO_0000198341

Regions

Domain16 – 156141CheW-like

Experimental info

Sequence conflict161G → R in AAA23575. Ref.5

Secondary structure

................................ 167
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A964 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: 9CE642E5F4E91BFF

FASTA16718,084
        10         20         30         40         50         60 
MTGMTNVTKL ASEPSGQEFL VFTLGDEEYG IDILKVQEIR GYDQVTRIAN TPAFIKGVTN 

        70         80         90        100        110        120 
LRGVIVPIVD LRIKFSQVDV DYNDNTVVIV LNLGQRVVGI VVDGVSDVLS LTAEQIRPAP 

       130        140        150        160 
EFAVTLSTEY LTGLGALGDR MLILVNIEKL LNSEEMALLD SAASEVA 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence corresponding to five chemotaxis genes in Escherichia coli."
Mutoh N., Simon M.I.
J. Bacteriol. 165:161-166(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Tandem translation starts in the cheA locus of Escherichia coli."
Kofoid E.C., Parkinson J.S.
J. Bacteriol. 173:2116-2119(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
[6]"Bacterial chemotaxis signaling complexes: formation of a CheA/CheW complex enhances autophosphorylation and affinity for CheY."
McNally D.F., Matsumura P.
Proc. Natl. Acad. Sci. U.S.A. 88:6269-6273(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M13462 Genomic DNA. Translation: AAA23565.1.
U00096 Genomic DNA. Translation: AAC74957.1.
AP009048 Genomic DNA. Translation: BAA15703.1.
M34669 Genomic DNA. Translation: AAA23575.1.
PIRQRECCW. B25195.
RefSeqNP_416401.1. NC_000913.3.
YP_490149.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2HO9NMR-A1-167[»]
ProteinModelPortalP0A964.
SMRP0A964. Positions 1-167.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48236N.
IntActP0A964. 31 interactions.
MINTMINT-1283783.
STRING511145.b1887.

Proteomic databases

PaxDbP0A964.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74957; AAC74957; b1887.
BAA15703; BAA15703; BAA15703.
GeneID12932044.
946400.
KEGGecj:Y75_p1863.
eco:b1887.
PATRIC32119101. VBIEscCol129921_1968.

Organism-specific databases

EchoBASEEB0147.
EcoGeneEG10149. cheW.

Phylogenomic databases

eggNOGCOG0835.
HOGENOMHOG000254712.
KOK03408.
OMAVTYNDNT.
OrthoDBEOG60CWQ7.
PhylomeDBP0A964.

Enzyme and pathway databases

BioCycEcoCyc:CHEW-MONOMER.
ECOL316407:JW1876-MONOMER.

Gene expression databases

GenevestigatorP0A964.

Family and domain databases

InterProIPR002545. CheW.
[Graphical view]
PfamPF01584. CheW. 1 hit.
[Graphical view]
SMARTSM00260. CheW. 1 hit.
[Graphical view]
SUPFAMSSF50341. SSF50341. 1 hit.
PROSITEPS50851. CHEW. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP0A964.
PROP0A964.

Entry information

Entry nameCHEW_ECOLI
AccessionPrimary (citable) accession number: P0A964
Secondary accession number(s): P07365
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene