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Protein

Glutamate-pyruvate aminotransferase AlaA

Gene

alaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of alanine.1 Publication

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Kineticsi

  1. KM=0.55 mM for pyruvate (at 37 degrees Celsius and pH 8.5)1 Publication
  2. KM=4.9 mM for alanine (at 37 degrees Celsius and pH 8.5)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei41Substrate; via amide nitrogenBy similarity1
    Binding sitei179SubstrateBy similarity1
    Binding sitei378SubstrateBy similarity1

    GO - Molecular functioni

    • L-alanine:2-oxoglutarate aminotransferase activity Source: UniProtKB
    • pyridoxal phosphate binding Source: EcoCyc
    • transaminase activity Source: EcoliWiki

    GO - Biological processi

    • alanine biosynthetic process Source: EcoliWiki
    • cellular response to DNA damage stimulus Source: EcoCyc
    • D-alanine biosynthetic process Source: UniProtKB
    • L-alanine biosynthetic process from pyruvate Source: EcoCyc
    • response to antibiotic Source: EcoCyc
    Complete GO annotation...

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciEcoCyc:G7184-MONOMER.
    ECOL316407:JW2287-MONOMER.
    MetaCyc:G7184-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate-pyruvate aminotransferase AlaA (EC:2.6.1.2)
    Gene namesi
    Name:alaA
    Synonyms:yfbQ
    Ordered Locus Names:b2290, JW2287
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG14101. alaA.

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001238661 – 405Glutamate-pyruvate aminotransferase AlaAAdd BLAST405

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei240N6-(pyridoxal phosphate)lysineBy similarity1

    Proteomic databases

    PaxDbiP0A959.
    PRIDEiP0A959.

    Expressioni

    Inductioni

    Modestly repressed by alanine and leucine via Lrp.1 Publication

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    DIPiDIP-11970N.
    IntActiP0A959. 10 interactors.
    STRINGi511145.b2290.

    Structurei

    Secondary structure

    1405
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi8 – 10Combined sources3
    Helixi20 – 30Combined sources11
    Helixi44 – 46Combined sources3
    Helixi52 – 61Combined sources10
    Helixi62 – 64Combined sources3
    Helixi75 – 86Combined sources12
    Turni87 – 89Combined sources3
    Helixi95 – 97Combined sources3
    Beta strandi98 – 103Combined sources6
    Helixi104 – 113Combined sources10
    Beta strandi121 – 127Combined sources7
    Helixi130 – 138Combined sources9
    Beta strandi142 – 148Combined sources7
    Helixi150 – 152Combined sources3
    Helixi158 – 163Combined sources6
    Beta strandi169 – 177Combined sources9
    Turni179 – 181Combined sources3
    Helixi187 – 199Combined sources13
    Beta strandi203 – 207Combined sources5
    Turni209 – 212Combined sources4
    Helixi223 – 226Combined sources4
    Beta strandi232 – 238Combined sources7
    Turni239 – 242Combined sources4
    Helixi245 – 247Combined sources3
    Beta strandi250 – 256Combined sources7
    Helixi259 – 261Combined sources3
    Helixi262 – 274Combined sources13
    Helixi281 – 284Combined sources4
    Helixi285 – 290Combined sources6
    Helixi297 – 300Combined sources4
    Helixi305 – 319Combined sources15
    Beta strandi329 – 334Combined sources6
    Helixi340 – 343Combined sources4
    Helixi348 – 359Combined sources12
    Beta strandi360 – 362Combined sources3
    Helixi366 – 369Combined sources4
    Beta strandi372 – 380Combined sources9
    Helixi385 – 400Combined sources16

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4CVQX-ray2.11A/B1-405[»]
    ProteinModelPortaliP0A959.
    SMRiP0A959.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105CHM. Bacteria.
    COG0436. LUCA.
    HOGENOMiHOG000223042.
    InParanoidiP0A959.
    KOiK14260.
    OMAiNVPGQWA.
    PhylomeDBiP0A959.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A959-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSPIEKSSKL ENVCYDIRGP VLKEAKRLEE EGNKVLKLNI GNPAPFGFDA
    60 70 80 90 100
    PDEILVDVIR NLPTAQGYCD SKGLYSARKA IMQHYQARGM RDVTVEDIYI
    110 120 130 140 150
    GNGVSELIVQ AMQALLNSGD EMLVPAPDYP LWTAAVSLSS GKAVHYLCDE
    160 170 180 190 200
    SSDWFPDLDD IRAKITPRTR GIVIINPNNP TGAVYSKELL MEIVEIARQH
    210 220 230 240 250
    NLIIFADEIY DKILYDDAEH HSIAPLAPDL LTITFNGLSK TYRVAGFRQG
    260 270 280 290 300
    WMVLNGPKKH AKGYIEGLEM LASMRLCANV PAQHAIQTAL GGYQSISEFI
    310 320 330 340 350
    TPGGRLYEQR NRAWELINDI PGVSCVKPRG ALYMFPKIDA KRFNIHDDQK
    360 370 380 390 400
    MVLDFLLQEK VLLVQGTAFN WPWPDHFRIV TLPRVDDIEL SLSKFARFLS

    GYHQL
    Length:405
    Mass (Da):45,517
    Last modified:July 19, 2005 - v1
    Checksum:i6A5E78876CC3C388
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC75350.1.
    AP009048 Genomic DNA. Translation: BAA16127.1.
    PIRiH65000.
    RefSeqiNP_416793.1. NC_000913.3.
    WP_000074527.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75350; AAC75350; b2290.
    BAA16127; BAA16127; BAA16127.
    GeneIDi946772.
    KEGGiecj:JW2287.
    eco:b2290.
    PATRICi32119949. VBIEscCol129921_2384.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC75350.1.
    AP009048 Genomic DNA. Translation: BAA16127.1.
    PIRiH65000.
    RefSeqiNP_416793.1. NC_000913.3.
    WP_000074527.1. NZ_LN832404.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    4CVQX-ray2.11A/B1-405[»]
    ProteinModelPortaliP0A959.
    SMRiP0A959.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    DIPiDIP-11970N.
    IntActiP0A959. 10 interactors.
    STRINGi511145.b2290.

    Proteomic databases

    PaxDbiP0A959.
    PRIDEiP0A959.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75350; AAC75350; b2290.
    BAA16127; BAA16127; BAA16127.
    GeneIDi946772.
    KEGGiecj:JW2287.
    eco:b2290.
    PATRICi32119949. VBIEscCol129921_2384.

    Organism-specific databases

    EchoBASEiEB3854.
    EcoGeneiEG14101. alaA.

    Phylogenomic databases

    eggNOGiENOG4105CHM. Bacteria.
    COG0436. LUCA.
    HOGENOMiHOG000223042.
    InParanoidiP0A959.
    KOiK14260.
    OMAiNVPGQWA.
    PhylomeDBiP0A959.

    Enzyme and pathway databases

    BioCyciEcoCyc:G7184-MONOMER.
    ECOL316407:JW2287-MONOMER.
    MetaCyc:G7184-MONOMER.

    Miscellaneous databases

    PROiP0A959.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiALAA_ECOLI
    AccessioniPrimary (citable) accession number: P0A959
    Secondary accession number(s): P77727
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 19, 2005
    Last sequence update: July 19, 2005
    Last modified: November 2, 2016
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.