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P0A959

- ALAA_ECOLI

UniProt

P0A959 - ALAA_ECOLI

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Protein
Glutamate-pyruvate aminotransferase AlaA
Gene
alaA, yfbQ, b2290, JW2287
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of alanine.1 Publication

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Pyridoxal phosphate By similarity.

Kineticsi

  1. KM=0.55 mM for pyruvate (at 37 degrees Celsius and pH 8.5)1 Publication
  2. KM=4.9 mM for alanine (at 37 degrees Celsius and pH 8.5)

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411Substrate; via amide nitrogen By similarity
Binding sitei179 – 1791Substrate By similarity
Binding sitei378 – 3781Substrate By similarity

GO - Molecular functioni

  1. L-alanine:2-oxoglutarate aminotransferase activity Source: UniProtKB
  2. pyridoxal phosphate binding Source: InterPro
  3. transaminase activity Source: EcoliWiki

GO - Biological processi

  1. D-alanine biosynthetic process Source: UniProtKB
  2. L-alanine biosynthetic process from pyruvate Source: EcoCyc
  3. alanine biosynthetic process Source: EcoliWiki
  4. cellular response to DNA damage stimulus Source: EcoCyc
  5. response to antibiotic Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:G7184-MONOMER.
ECOL316407:JW2287-MONOMER.
MetaCyc:G7184-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate-pyruvate aminotransferase AlaA (EC:2.6.1.2)
Gene namesi
Name:alaA
Synonyms:yfbQ
Ordered Locus Names:b2290, JW2287
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG14101. alaA.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 405405Glutamate-pyruvate aminotransferase AlaA
PRO_0000123866Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei240 – 2401N6-(pyridoxal phosphate)lysine By similarity

Proteomic databases

PaxDbiP0A959.
PRIDEiP0A959.

Expressioni

Inductioni

Modestly repressed by alanine and leucine via Lrp.1 Publication

Gene expression databases

GenevestigatoriP0A959.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

DIPiDIP-11970N.
IntActiP0A959. 10 interactions.
STRINGi511145.b2290.

Structurei

3D structure databases

ProteinModelPortaliP0A959.
SMRiP0A959. Positions 6-405.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0436.
HOGENOMiHOG000223042.
KOiK14260.
OMAiYQARDMR.
OrthoDBiEOG6X9MJ6.
PhylomeDBiP0A959.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A959-1 [UniParc]FASTAAdd to Basket

« Hide

MSPIEKSSKL ENVCYDIRGP VLKEAKRLEE EGNKVLKLNI GNPAPFGFDA    50
PDEILVDVIR NLPTAQGYCD SKGLYSARKA IMQHYQARGM RDVTVEDIYI 100
GNGVSELIVQ AMQALLNSGD EMLVPAPDYP LWTAAVSLSS GKAVHYLCDE 150
SSDWFPDLDD IRAKITPRTR GIVIINPNNP TGAVYSKELL MEIVEIARQH 200
NLIIFADEIY DKILYDDAEH HSIAPLAPDL LTITFNGLSK TYRVAGFRQG 250
WMVLNGPKKH AKGYIEGLEM LASMRLCANV PAQHAIQTAL GGYQSISEFI 300
TPGGRLYEQR NRAWELINDI PGVSCVKPRG ALYMFPKIDA KRFNIHDDQK 350
MVLDFLLQEK VLLVQGTAFN WPWPDHFRIV TLPRVDDIEL SLSKFARFLS 400
GYHQL 405
Length:405
Mass (Da):45,517
Last modified:July 19, 2005 - v1
Checksum:i6A5E78876CC3C388
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC75350.1.
AP009048 Genomic DNA. Translation: BAA16127.1.
PIRiH65000.
RefSeqiNP_416793.1. NC_000913.3.
YP_490532.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75350; AAC75350; b2290.
BAA16127; BAA16127; BAA16127.
GeneIDi12933976.
946772.
KEGGiecj:Y75_p2256.
eco:b2290.
PATRICi32119949. VBIEscCol129921_2384.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC75350.1 .
AP009048 Genomic DNA. Translation: BAA16127.1 .
PIRi H65000.
RefSeqi NP_416793.1. NC_000913.3.
YP_490532.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0A959.
SMRi P0A959. Positions 6-405.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-11970N.
IntActi P0A959. 10 interactions.
STRINGi 511145.b2290.

Proteomic databases

PaxDbi P0A959.
PRIDEi P0A959.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75350 ; AAC75350 ; b2290 .
BAA16127 ; BAA16127 ; BAA16127 .
GeneIDi 12933976.
946772.
KEGGi ecj:Y75_p2256.
eco:b2290.
PATRICi 32119949. VBIEscCol129921_2384.

Organism-specific databases

EchoBASEi EB3854.
EcoGenei EG14101. alaA.

Phylogenomic databases

eggNOGi COG0436.
HOGENOMi HOG000223042.
KOi K14260.
OMAi YQARDMR.
OrthoDBi EOG6X9MJ6.
PhylomeDBi P0A959.

Enzyme and pathway databases

BioCyci EcoCyc:G7184-MONOMER.
ECOL316407:JW2287-MONOMER.
MetaCyc:G7184-MONOMER.

Miscellaneous databases

PROi P0A959.

Gene expression databases

Genevestigatori P0A959.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF00155. Aminotran_1_2. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Genetics and regulation of the major enzymes of alanine synthesis in Escherichia coli."
    Kim S.H., Schneider B.L., Reitzer L.
    J. Bacteriol. 192:5304-5311(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS AN AMINOTRANSFERASE AND IN ALANINE BIOSYNTHESIS, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, INDUCTION, NOMENCLATURE.

Entry informationi

Entry nameiALAA_ECOLI
AccessioniPrimary (citable) accession number: P0A959
Secondary accession number(s): P77727
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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