P0A957 (ALKH_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
KHG/KDPG aldolase

Including the following 2 domains:

1. 4-hydroxy-2-oxoglutarate aldolase
   EC=4.1.3.16
   Alternative name(s):
   2-keto-4-hydroxyglutarate aldolase
   Short name=KHG-aldolase
2. 2-dehydro-3-deoxy-phosphogluconate aldolase
   EC=4.1.2.14
   Alternative name(s):
   2-keto-3-deoxy-6-phosphogluconate aldolase
   Short name=KDPG-aldolase
   Phospho-2-dehydro-3-deoxygluconate aldolase
   Phospho-2-keto-3-deoxygluconate aldolase

Gene names

Name:	eda
Ordered Locus Names:	Z2902, ECs2560

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate. 2-dehydro-3-deoxy-D-gluconate 6-phosphate = pyruvate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate: step 2/2. Carbohydrate metabolism; glyoxylate and dicarboxylate metabolism.
Subunit structure	Homotrimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	It also catalyzes the beta-decarboxylation of oxaloacetate By similarity.
Sequence similarities	Belongs to the KHG/KDPG aldolase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	2-dehydro-3-deoxy-phosphogluconate aldolase activity Inferred from electronic annotation. Source: EC 4-hydroxy-2-oxoglutarate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	KHG/KDPG aldolase		PRO_0000201038
Sites
	Active site	45	1	By similarity
	Active site	49	1	By similarity
	Active site	133	1	Schiff-base intermediate with KHG or pyruvate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A957 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: CC8D51B50480D0B7	FASTA	213	22,284
	10 20 30 40 50 60 MKNWKTSAES ILTTGPVVPV IVVKKLEHAV PMAKALVAGG VRVLEVTLRT ECAVDAIRAI 70 80 90 100 110 120 AKEVPEAIVG AGTVLNPQQL AEVTEAGAQF AISPGLTEPL LKAATEGTIP LIPGISTVSE 130 140 150 160 170 180 LMLGMDYGLK EFKFFPAEAN GGVKALQAIA GPFSQVRFCP TGGISPANYR DYLALKSVLC 190 200 210 IGGSWLVPAD ALEAGDYDRI TKLAREAVEG AKL

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG56840.1. BA000007 Genomic DNA. Translation: BAB35983.1.
PIR	D85797. H90948.
RefSeq	NP_288287.1. NC_002655.2. NP_310587.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P0A957.
SMR	P0A957. Positions 1-213.
ModBase	Search...
Protein-protein interaction databases
STRING	155864.Z2902.
Proteomic databases
PRIDE	P0A957.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAG56840; AAG56840; Z2902. BAB35983; BAB35983; BAB35983.
GeneID	912650. 961823.
KEGG	ece:Z2902. ecs:ECs2560.
PATRIC	18354490. VBIEscCol44059_2456.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0800.
HOGENOM	HOG000233114.
KO	K01625.
OMA	AQFAVSP.
ProtClustDB	PRK05718.
Enzyme and pathway databases
BioCyc	ECOL386585:GJFA-2534-MONOMER.
UniPathway	UPA00227. UPA00856; UER00829.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR000887. Aldlse_KDPG_KHG. IPR013785. Aldolase_TIM. [Graphical view]
Pfam	PF01081. Aldolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01182. eda. 1 hit.
PROSITE	PS00159. ALDOLASE_KDPG_KHG_1. 1 hit. PS00160. ALDOLASE_KDPG_KHG_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALKH_ECO57

Accession

Primary (citable) accession number: P0A957
Secondary accession number(s): P10177

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program