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Protein

KHG/KDPG aldolase

Gene

eda

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate.
2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate.

Pathwayi: 2-dehydro-3-deoxy-D-gluconate degradation

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. KHG/KDPG aldolase (eda)
This subpathway is part of the pathway 2-dehydro-3-deoxy-D-gluconate degradation, which is itself part of Carbohydrate acid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro-3-deoxy-D-gluconate, the pathway 2-dehydro-3-deoxy-D-gluconate degradation and in Carbohydrate acid metabolism.

Pathwayi: glyoxylate and dicarboxylate metabolism

This protein is involved in the pathway glyoxylate and dicarboxylate metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway glyoxylate and dicarboxylate metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei45PROSITE-ProRule annotation1
Active sitei49PROSITE-ProRule annotation1
Active sitei133Schiff-base intermediate with KHG or pyruvatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

BioCyciECOO157:EDA-MONOMER.
UniPathwayiUPA00227.
UPA00856; UER00829.

Names & Taxonomyi

Protein namesi
Recommended name:
KHG/KDPG aldolase
Including the following 2 domains:
4-hydroxy-2-oxoglutarate aldolase (EC:4.1.3.16)
Alternative name(s):
2-keto-4-hydroxyglutarate aldolase
Short name:
KHG-aldolase
2-dehydro-3-deoxy-phosphogluconate aldolase (EC:4.1.2.14)
Alternative name(s):
2-keto-3-deoxy-6-phosphogluconate aldolase
Short name:
KDPG-aldolase
Phospho-2-dehydro-3-deoxygluconate aldolase
Phospho-2-keto-3-deoxygluconate aldolase
Gene namesi
Name:eda
Ordered Locus Names:Z2902, ECs2560
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002010381 – 213KHG/KDPG aldolaseAdd BLAST213

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi155864.Z2902.

Structurei

3D structure databases

ProteinModelPortaliP0A957.
SMRiP0A957.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the KHG/KDPG aldolase family.Curated

Phylogenomic databases

eggNOGiENOG4108UHU. Bacteria.
COG0800. LUCA.
HOGENOMiHOG000233114.
KOiK01625.
OMAiFLISPGL.

Family and domain databases

CDDicd00452. KDPG_aldolase. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR000887. Aldlse_KDPG_KHG.
IPR013785. Aldolase_TIM.
IPR031337. KDPG/KHG_AS_1.
IPR031338. KDPG/KHG_AS_2.
[Graphical view]
PfamiPF01081. Aldolase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01182. eda. 1 hit.
PROSITEiPS00159. ALDOLASE_KDPG_KHG_1. 1 hit.
PS00160. ALDOLASE_KDPG_KHG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A957-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNWKTSAES ILTTGPVVPV IVVKKLEHAV PMAKALVAGG VRVLEVTLRT
60 70 80 90 100
ECAVDAIRAI AKEVPEAIVG AGTVLNPQQL AEVTEAGAQF AISPGLTEPL
110 120 130 140 150
LKAATEGTIP LIPGISTVSE LMLGMDYGLK EFKFFPAEAN GGVKALQAIA
160 170 180 190 200
GPFSQVRFCP TGGISPANYR DYLALKSVLC IGGSWLVPAD ALEAGDYDRI
210
TKLAREAVEG AKL
Length:213
Mass (Da):22,284
Last modified:July 19, 2005 - v1
Checksum:iCC8D51B50480D0B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56840.1.
BA000007 Genomic DNA. Translation: BAB35983.1.
PIRiD85797.
H90948.
RefSeqiNP_310587.1. NC_002695.1.
WP_000800512.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG56840; AAG56840; Z2902.
BAB35983; BAB35983; BAB35983.
GeneIDi912650.
KEGGiece:Z2902.
ecs:ECs2560.
PATRICi18354490. VBIEscCol44059_2456.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG56840.1.
BA000007 Genomic DNA. Translation: BAB35983.1.
PIRiD85797.
H90948.
RefSeqiNP_310587.1. NC_002695.1.
WP_000800512.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliP0A957.
SMRiP0A957.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z2902.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG56840; AAG56840; Z2902.
BAB35983; BAB35983; BAB35983.
GeneIDi912650.
KEGGiece:Z2902.
ecs:ECs2560.
PATRICi18354490. VBIEscCol44059_2456.

Phylogenomic databases

eggNOGiENOG4108UHU. Bacteria.
COG0800. LUCA.
HOGENOMiHOG000233114.
KOiK01625.
OMAiFLISPGL.

Enzyme and pathway databases

UniPathwayiUPA00227.
UPA00856; UER00829.
BioCyciECOO157:EDA-MONOMER.

Family and domain databases

CDDicd00452. KDPG_aldolase. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR000887. Aldlse_KDPG_KHG.
IPR013785. Aldolase_TIM.
IPR031337. KDPG/KHG_AS_1.
IPR031338. KDPG/KHG_AS_2.
[Graphical view]
PfamiPF01081. Aldolase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01182. eda. 1 hit.
PROSITEiPS00159. ALDOLASE_KDPG_KHG_1. 1 hit.
PS00160. ALDOLASE_KDPG_KHG_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALKH_ECO57
AccessioniPrimary (citable) accession number: P0A957
Secondary accession number(s): P10177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: November 2, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

It also catalyzes the beta-decarboxylation of oxaloacetate.By similarity

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.