Reviewed, UniProtKB/Swiss-Prot P0A952 (ATDA_ECO57)

Names and origin

Protein names

Recommended name:
    Spermidine N(1)-acetyltransferase
    EC=2.3.1.57
Alternative name(s):
    Diamine acetyltransferase
    SAT

Gene names

Name:	speG
Ordered Locus Names:	Z2571, ECs2290

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Regulates polyamine concentration by their degradation. Acts on spermidine, forming N(1)- and N(8)-acetylspermidine By similarity.
Catalytic activity	Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the acetyltransferase family. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diamine N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 186	185	Spermidine N(1)-acetyltransferase		PRO_0000074589
Regions
	Domain	7 – 167	161	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	P0A952-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 27297083B93B3752	FASTA	186	21,887
	10 20 30 40 50 60 MPSAHSVKLR PLEREDLRYV HQLDNNASVM RYWFEEPYEA FVELSDLYDK HIHDQSERRF 70 80 90 100 110 120 VVECDGEKAG LVELVEINHV HRRAEFQIII SPEYQGKGLA TRAAKLAMDY GFTVLNLYKL 130 140 150 160 170 180 YLIVDKENEK AIHIYRKLGF SVEGELMHEF FINGQYRNAI RMCIFQHQYL AEHKTPGQTL LKPTAQ

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG56571.1. BA000007 Genomic DNA. Translation: BAB35713.1.
PIR	B90915. G85763.
RefSeq	NP_288019.1. NP_310317.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	915270. 961524.
GenomeReviews	Gene locus Z2571 in contig AE005174_GR. Gene locus ECs2290 in contig BA000007_GR.
KEGG	ece:Z2571. ecs:ECs2290.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A952.
OMA	DEFFVNG.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS2290-MON.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GCN5-rel_AcTrfase. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATDA_ECO57

Accession

Primary (citable) accession number: P0A952
Secondary accession number(s): P37354

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 32 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)