P0A951 (ATDA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermidine N(1)-acetyltransferase
EC=2.3.1.57

Alternative name(s):
Diamine acetyltransferase
SAT

Gene names

Name:	speG
Ordered Locus Names:	b1584, JW1576

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	186 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulates polyamine concentration by their degradation. Acts on spermidine, forming N(1)- and N(8)-acetylspermidine.
Catalytic activity	Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the acetyltransferase family. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	diamine N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 186	185	Spermidine N(1)-acetyltransferase		PRO_0000074588
Regions
	Domain	7 – 167	161	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	P0A951 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 27297083B93B3752	FASTA	186	21,887
	10 20 30 40 50 60 MPSAHSVKLR PLEREDLRYV HQLDNNASVM RYWFEEPYEA FVELSDLYDK HIHDQSERRF 70 80 90 100 110 120 VVECDGEKAG LVELVEINHV HRRAEFQIII SPEYQGKGLA TRAAKLAMDY GFTVLNLYKL 130 140 150 160 170 180 YLIVDKENEK AIHIYRKLGF SVEGELMHEF FINGQYRNAI RMCIFQHQYL AEHKTPGQTL LKPTAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Properties and structure of spermidine acetyltransferase in Escherichia coli." Fukuchi J., Kashiwagi K., Takio K., Igarashi K. J. Biol. Chem. 269:22581-22585(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D25276 Genomic DNA. Translation: BAA04966.1. U00096 Genomic DNA. Translation: AAC74656.1. AP009048 Genomic DNA. Translation: BAA15286.1.
PIR	A55345.
RefSeq	NP_416101.1. NC_000913.2. YP_489847.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A951.
SMR	P0A951. Positions 7-170.
ModBase	Search...
Protein-protein interaction databases
IntAct	P0A951. 11 interactions.
STRING	511145.b1584.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74656; AAC74656; b1584. BAA15286; BAA15286; BAA15286.
GeneID	12931240. 946117.
KEGG	ecj:Y75_p1560. eco:b1584.
PATRIC	32118470. VBIEscCol129921_1655.
Organism-specific databases
EchoBASE	EB2341.
EcoGene	EG12447. speG.
Phylogenomic databases
eggNOG	COG1670.
HOGENOM	HOG000078522.
KO	K00657.
OMA	MVEGELI.
ProtClustDB	PRK15130.
Enzyme and pathway databases
BioCyc	EcoCyc:SPERMACTRAN-MONOMER. ECOL316407:JW1576-MONOMER. MetaCyc:SPERMACTRAN-MONOMER.
Gene expression databases
Genevestigator	P0A951.
Family and domain databases
Gene3D	3.40.630.30. 1 hit.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GNAT_dom. [Graphical view]
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATDA_ECOLI

Accession

Primary (citable) accession number: P0A951
Secondary accession number(s): P37354

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program