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Protein

Ribosomal-protein-alanine acetyltransferase

Gene

rimJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

This enzyme acetylates the N-terminal alanine of ribosomal protein S5. Plays also a role in the temperature regulation of pap pilin transcription.

Catalytic activityi

Acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N-acetyl-L-alanine.

GO - Molecular functioni

  • N-acetyltransferase activity Source: EcoliWiki
  • ribosomal-protein-alanine N-acetyltransferase activity Source: EcoliWiki

GO - Biological processi

  • cellular protein modification process Source: EcoliWiki
  • N-terminal protein amino acid acetylation Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:EG10851-MONOMER.
ECOL316407:JW1053-MONOMER.
MetaCyc:EG10851-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal-protein-alanine acetyltransferase (EC:2.3.1.128)
Alternative name(s):
Acetylating enzyme for N-terminal of ribosomal protein S5
Gene namesi
Name:rimJ
Ordered Locus Names:b1066, JW1053
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10851. rimJ.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: EcoliWiki
  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 194194Ribosomal-protein-alanine acetyltransferasePRO_0000074567Add
BLAST

Proteomic databases

EPDiP0A948.
PaxDbiP0A948.
PRIDEiP0A948.

Interactioni

Protein-protein interaction databases

BioGridi4260076. 16 interactions.
DIPiDIP-35985N.
IntActiP0A948. 17 interactions.
STRINGi511145.b1066.

Structurei

3D structure databases

ProteinModelPortaliP0A948.
SMRiP0A948. Positions 20-192.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 188171N-acetyltransferasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105D9U. Bacteria.
COG1670. LUCA.
HOGENOMiHOG000078527.
InParanoidiP0A948.
KOiK03790.
OMAiLCINGVW.
OrthoDBiEOG6DZF4Q.
PhylomeDBiP0A948.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF13302. Acetyltransf_3. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A948-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGYRSNVPK VRLTTDRLVV RLVHDRDAWR LADYYAENRH FLKPWEPVRD
60 70 80 90 100
ESHCYPSGWQ ARLGMINEFH KQGSAFYFGL FDPDEKEIIG VANFSNVVRG
110 120 130 140 150
SFHACYLGYS IGQKWQGKGL MFEALTAAIR YMQRTQHIHR IMANYMPHNK
160 170 180 190
RSGDLLARLG FEKEGYAKDY LLIDGQWRDH VLTALTTPDW TPGR
Length:194
Mass (Da):22,688
Last modified:July 19, 2005 - v1
Checksum:i1DCC58BD1C06AB61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06118 Genomic DNA. Translation: CAA29490.1.
M99278 Genomic DNA. Translation: AAA24549.1.
U00096 Genomic DNA. Translation: AAC74150.1.
AP009048 Genomic DNA. Translation: BAA35874.1.
PIRiS01084.
RefSeqiNP_415584.1. NC_000913.3.
WP_000468186.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74150; AAC74150; b1066.
BAA35874; BAA35874; BAA35874.
GeneIDi946910.
KEGGiecj:JW1053.
eco:b1066.
PATRICi32117371. VBIEscCol129921_1108.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06118 Genomic DNA. Translation: CAA29490.1.
M99278 Genomic DNA. Translation: AAA24549.1.
U00096 Genomic DNA. Translation: AAC74150.1.
AP009048 Genomic DNA. Translation: BAA35874.1.
PIRiS01084.
RefSeqiNP_415584.1. NC_000913.3.
WP_000468186.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A948.
SMRiP0A948. Positions 20-192.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260076. 16 interactions.
DIPiDIP-35985N.
IntActiP0A948. 17 interactions.
STRINGi511145.b1066.

Proteomic databases

EPDiP0A948.
PaxDbiP0A948.
PRIDEiP0A948.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74150; AAC74150; b1066.
BAA35874; BAA35874; BAA35874.
GeneIDi946910.
KEGGiecj:JW1053.
eco:b1066.
PATRICi32117371. VBIEscCol129921_1108.

Organism-specific databases

EchoBASEiEB0844.
EcoGeneiEG10851. rimJ.

Phylogenomic databases

eggNOGiENOG4105D9U. Bacteria.
COG1670. LUCA.
HOGENOMiHOG000078527.
InParanoidiP0A948.
KOiK03790.
OMAiLCINGVW.
OrthoDBiEOG6DZF4Q.
PhylomeDBiP0A948.

Enzyme and pathway databases

BioCyciEcoCyc:EG10851-MONOMER.
ECOL316407:JW1053-MONOMER.
MetaCyc:EG10851-MONOMER.

Miscellaneous databases

PROiP0A948.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
[Graphical view]
PfamiPF13302. Acetyltransf_3. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and nucleotide sequencing of the genes rimI and rimJ which encode enzymes acetylating ribosomal proteins S18 and S5 of Escherichia coli K12."
    Yoshikawa A., Isono S., Sheback A., Isono K.
    Mol. Gen. Genet. 209:481-488(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Thermoregulation of the pap operon: evidence for the involvement of RimJ, the N-terminal acetylase of ribosomal protein S5."
    White-Ziegler C.A., Low D.A.
    J. Bacteriol. 174:7003-7012(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiRIMJ_ECOLI
AccessioniPrimary (citable) accession number: P0A948
Secondary accession number(s): P09454
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: May 11, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.