P0A944 (RIMI_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal-protein-alanine acetyltransferase
EC=2.3.1.128

Alternative name(s):
Acetylating enzyme for N-terminal of ribosomal protein S18

Gene names

Name:	rimI
Ordered Locus Names:	b4373, JW4335

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme acetylates the N-terminal alanine of ribosomal protein S18.
Catalytic activity	Acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N-acetyl-L-alanine.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the acetyltransferase family. RimI subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	N-terminal protein amino acid acetylation Inferred from mutant phenotype PubMed 6991870. Source: EcoliWiki
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ribosomal-protein-alanine N-acetyltransferase activity Inferred from mutant phenotype PubMed 6991870. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 148	148	Ribosomal-protein-alanine acetyltransferase		PRO_0000074561
Regions
	Domain	2 – 147	146	N-acetyltransferase
Experimental info
	Sequence conflict	143 – 148	6	ALPISM → RCQSVCNTRWNNEVGLDFL in CAA29489. Ref.1
	Sequence conflict	143 – 148	6	ALPISM → RCQSVCNTRWNNEVGLDFL in AAA97269. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0A944 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 46A8412D38D42F86	FASTA	148	16,610
	10 20 30 40 50 60 MNTISSLETT DLPAAYHIEQ RAHAFPWSEK TFASNQGERY LNFQLTQNGK MAAFAITQVV 70 80 90 100 110 120 LDEATLFNIA VDPDYQRQGL GRALLEHLID ELEKRGVATL WLEVRASNAA AIALYESLGF 130 140 NEATIRRNYY PTTDGREDAI IMALPISM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and nucleotide sequencing of the genes rimI and rimJ which encode enzymes acetylating ribosomal proteins S18 and S5 of Escherichia coli K12." Yoshikawa A., Isono S., Sheback A., Isono K. Mol. Gen. Genet. 209:481-488(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO C-TERMINUS. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Identification, isolation, and overexpression of the gene encoding the psi subunit of DNA polymerase III holoenzyme." Carter J.R., Franden M.A., Aebersold R.H., McHenry C.S. J. Bacteriol. 175:5604-5610(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X06117 Genomic DNA. Translation: CAA29489.1. U14003 Genomic DNA. Translation: AAA97269.1. U00096 Genomic DNA. Translation: AAC77326.1. AP009048 Genomic DNA. Translation: BAE78361.1. L05387 Unassigned DNA. Translation: AAA03077.1.
PIR	D65252.
RefSeq	NP_418790.1. NC_000913.2. YP_492502.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A944.
SMR	P0A944. Positions 1-148.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48259N.
STRING	511145.b4373.
Proteomic databases
PRIDE	P0A944.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC77326; AAC77326; b4373. BAE78361; BAE78361; BAE78361.
GeneID	12933792. 948894.
KEGG	ecj:Y75_p4256. eco:b4373.
PATRIC	32124356. VBIEscCol129921_4518.
Organism-specific databases
EchoBASE	EB0843.
EcoGene	EG10850. rimI.
Phylogenomic databases
eggNOG	COG0456.
HOGENOM	HOG000078523.
KO	K03789.
OMA	ISQIEAC.
ProtClustDB	PRK09491.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10850-MONOMER. ECOL316407:JW4335-MONOMER. MetaCyc:EG10850-MONOMER.
Gene expression databases
Genevestigator	P0A944.
Family and domain databases
Gene3D	3.40.630.30. 1 hit.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000182. GNAT_dom. IPR006464. Rbsml_AcTrfase. [Graphical view]
Pfam	PF00583. Acetyltransf_1. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit.
TIGRFAMs	TIGR01575. rimI. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIMI_ECOLI

Accession

Primary (citable) accession number: P0A944
Secondary accession number(s): P09453, Q2M5U5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program