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Protein

Ribosomal-protein-alanine acetyltransferase

Gene

rimI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

This enzyme acetylates the N-terminal alanine of ribosomal protein S18.

Catalytic activityi

Acetyl-CoA + ribosomal-protein L-alanine = CoA + ribosomal-protein N-acetyl-L-alanine.

GO - Molecular functioni

  • N-acetyltransferase activity Source: EcoliWiki
  • ribosomal-protein-alanine N-acetyltransferase activity Source: EcoliWiki

GO - Biological processi

  • cellular protein modification process Source: EcoliWiki
  • N-terminal peptidyl-alanine acetylation Source: EcoliWiki
  • N-terminal protein amino acid acetylation Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciEcoCyc:EG10850-MONOMER.
ECOL316407:JW4335-MONOMER.
MetaCyc:EG10850-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal-protein-alanine acetyltransferase (EC:2.3.1.128)
Alternative name(s):
Acetylating enzyme for N-terminal of ribosomal protein S18
Gene namesi
Name:rimI
Ordered Locus Names:b4373, JW4335
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10850. rimI.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 148148Ribosomal-protein-alanine acetyltransferasePRO_0000074561Add
BLAST

Proteomic databases

PaxDbiP0A944.
PRIDEiP0A944.

Interactioni

Protein-protein interaction databases

BioGridi4262786. 3 interactions.
DIPiDIP-48259N.
IntActiP0A944. 1 interaction.
STRINGi511145.b4373.

Structurei

3D structure databases

ProteinModelPortaliP0A944.
SMRiP0A944. Positions 1-148.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 147146N-acetyltransferasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105KEG. Bacteria.
COG0456. LUCA.
HOGENOMiHOG000078523.
InParanoidiP0A944.
KOiK03789.
OMAiNICVHPD.
OrthoDBiEOG6D8B8B.
PhylomeDBiP0A944.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
IPR006464. Rbsml_AcTrfase.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
TIGRFAMsiTIGR01575. rimI. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A944-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTISSLETT DLPAAYHIEQ RAHAFPWSEK TFASNQGERY LNFQLTQNGK
60 70 80 90 100
MAAFAITQVV LDEATLFNIA VDPDYQRQGL GRALLEHLID ELEKRGVATL
110 120 130 140
WLEVRASNAA AIALYESLGF NEATIRRNYY PTTDGREDAI IMALPISM
Length:148
Mass (Da):16,610
Last modified:July 19, 2005 - v1
Checksum:i46A8412D38D42F86
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti143 – 1486ALPISM → RCQSVCNTRWNNEVGLDFL in CAA29489 (PubMed:2828880).Curated
Sequence conflicti143 – 1486ALPISM → RCQSVCNTRWNNEVGLDFL in AAA97269 (PubMed:7610040).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06117 Genomic DNA. Translation: CAA29489.1.
U14003 Genomic DNA. Translation: AAA97269.1.
U00096 Genomic DNA. Translation: AAC77326.1.
AP009048 Genomic DNA. Translation: BAE78361.1.
L05387 Unassigned DNA. Translation: AAA03077.1.
PIRiD65252.
RefSeqiNP_418790.1. NC_000913.3.
WP_001092461.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77326; AAC77326; b4373.
BAE78361; BAE78361; BAE78361.
GeneIDi948894.
KEGGiecj:JW4335.
eco:b4373.
PATRICi32124356. VBIEscCol129921_4518.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06117 Genomic DNA. Translation: CAA29489.1.
U14003 Genomic DNA. Translation: AAA97269.1.
U00096 Genomic DNA. Translation: AAC77326.1.
AP009048 Genomic DNA. Translation: BAE78361.1.
L05387 Unassigned DNA. Translation: AAA03077.1.
PIRiD65252.
RefSeqiNP_418790.1. NC_000913.3.
WP_001092461.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A944.
SMRiP0A944. Positions 1-148.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262786. 3 interactions.
DIPiDIP-48259N.
IntActiP0A944. 1 interaction.
STRINGi511145.b4373.

Proteomic databases

PaxDbiP0A944.
PRIDEiP0A944.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77326; AAC77326; b4373.
BAE78361; BAE78361; BAE78361.
GeneIDi948894.
KEGGiecj:JW4335.
eco:b4373.
PATRICi32124356. VBIEscCol129921_4518.

Organism-specific databases

EchoBASEiEB0843.
EcoGeneiEG10850. rimI.

Phylogenomic databases

eggNOGiENOG4105KEG. Bacteria.
COG0456. LUCA.
HOGENOMiHOG000078523.
InParanoidiP0A944.
KOiK03789.
OMAiNICVHPD.
OrthoDBiEOG6D8B8B.
PhylomeDBiP0A944.

Enzyme and pathway databases

BioCyciEcoCyc:EG10850-MONOMER.
ECOL316407:JW4335-MONOMER.
MetaCyc:EG10850-MONOMER.

Miscellaneous databases

PROiP0A944.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
IPR006464. Rbsml_AcTrfase.
[Graphical view]
PfamiPF00583. Acetyltransf_1. 1 hit.
[Graphical view]
SUPFAMiSSF55729. SSF55729. 1 hit.
TIGRFAMsiTIGR01575. rimI. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and nucleotide sequencing of the genes rimI and rimJ which encode enzymes acetylating ribosomal proteins S18 and S5 of Escherichia coli K12."
    Yoshikawa A., Isono S., Sheback A., Isono K.
    Mol. Gen. Genet. 209:481-488(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
    Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO C-TERMINUS.
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Identification, isolation, and overexpression of the gene encoding the psi subunit of DNA polymerase III holoenzyme."
    Carter J.R., Franden M.A., Aebersold R.H., McHenry C.S.
    J. Bacteriol. 175:5604-5610(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-30.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiRIMI_ECOLI
AccessioniPrimary (citable) accession number: P0A944
Secondary accession number(s): P09453, Q2M5U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: January 20, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.