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Protein

Membrane-bound lytic murein transglycosylase A

Gene

mltA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Degrades murein glycan strands and insoluble, high-molecular weight murein sacculi.

Catalytic activityi

Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.

pH dependencei

Optimum pH is 4.0-4.5.

Temperature dependencei

Loses rapidly its activity at temperatures above 30 degrees Celsius.

GO - Molecular functioni

  • exo-oligoalginate lyase activity Source: UniProtKB-EC
  • gellan lyase activity Source: UniProtKB-EC
  • hydrolase activity, hydrolyzing O-glycosyl compounds Source: EcoCyc
  • lytic transglycosylase activity Source: EcoliWiki

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan biosynthetic process Source: EcoCyc
  • peptidoglycan turnover Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciEcoCyc:G7457-MONOMER.
ECOL316407:JW2784-MONOMER.
MetaCyc:G7457-MONOMER.

Protein family/group databases

CAZyiGH102. Glycoside Hydrolase Family 102.

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-bound lytic murein transglycosylase A (EC:4.2.2.n1)
Alternative name(s):
Mlt38
Murein hydrolase A
Gene namesi
Name:mltA
Synonyms:mlt, ygdM
Ordered Locus Names:b2813, JW2784
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13085. mltA.

Subcellular locationi

GO - Cellular componenti

  • cell outer membrane Source: UniProtKB-SubCell
  • cytosol Source: EcoCyc
  • integral component of membrane Source: EcoCyc
  • integral component of plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20CuratedAdd BLAST20
ChainiPRO_000003278121 – 365Membrane-bound lytic murein transglycosylase AAdd BLAST345

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi21N-palmitoyl cysteineCurated1
Lipidationi21S-diacylglycerol cysteineCurated1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP0A935.
PRIDEiP0A935.

Interactioni

Subunit structurei

Forms a trimeric complex with MrcB/PonB and MipA in vitro.

Protein-protein interaction databases

BioGridi4261126. 187 interactors.
DIPiDIP-51238N.
IntActiP0A935. 1 interactor.
STRINGi511145.b2813.

Structurei

Secondary structure

1365
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi38 – 46Combined sources9
Helixi56 – 69Combined sources14
Helixi71 – 89Combined sources19
Helixi94 – 100Combined sources7
Beta strandi102 – 107Combined sources6
Turni109 – 112Combined sources4
Beta strandi116 – 123Combined sources8
Beta strandi125 – 131Combined sources7
Beta strandi138 – 142Combined sources5
Helixi148 – 150Combined sources3
Helixi153 – 157Combined sources5
Helixi163 – 165Combined sources3
Beta strandi166 – 171Combined sources6
Helixi173 – 182Combined sources10
Beta strandi185 – 188Combined sources4
Beta strandi190 – 192Combined sources3
Beta strandi196 – 203Combined sources8
Helixi211 – 217Combined sources7
Helixi223 – 225Combined sources3
Helixi228 – 236Combined sources9
Helixi240 – 247Combined sources8
Beta strandi254 – 259Combined sources6
Beta strandi276 – 279Combined sources4
Turni282 – 284Combined sources3
Beta strandi290 – 298Combined sources9
Beta strandi300 – 302Combined sources3
Beta strandi304 – 317Combined sources14
Beta strandi327 – 334Combined sources8
Helixi335 – 341Combined sources7
Beta strandi346 – 355Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AE0X-ray2.00X22-365[»]
2GAEX-ray2.50A22-365[»]
2PI8X-ray2.25A/B/C/D22-365[»]
2PICX-ray2.25A22-365[»]
2PJJX-ray2.46A22-365[»]
ProteinModelPortaliP0A935.
SMRiP0A935.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A935.

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CA3. Bacteria.
COG2821. LUCA.
HOGENOMiHOG000278098.
InParanoidiP0A935.
KOiK08304.
OMAiKREDMSM.
PhylomeDBiP0A935.

Family and domain databases

Gene3Di2.40.40.10. 2 hits.
InterProiIPR010611. 3D_dom.
IPR005300. Lytic_transglycosylase_MltA.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamiPF06725. 3D. 1 hit.
PF03562. MltA. 1 hit.
[Graphical view]
SMARTiSM00925. MltA. 1 hit.
[Graphical view]
SUPFAMiSSF50685. SSF50685. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A935-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGRWVKYLL MGTVVAMLAA CSSKPTDRGQ QYKDGKFTQP FSLVNQPDAV
60 70 80 90 100
GAPINAGDFA EQINHIRNSS PRLYGNQSNV YNAVQEWLRA GGDTRNMRQF
110 120 130 140 150
GIDAWQMEGA DNYGNVQFTG YYTPVIQARH TRQGEFQYPI YRMPPKRGRL
160 170 180 190 200
PSRAEIYAGA LSDKYILAYS NSLMDNFIMD VQGSGYIDFG DGSPLNFFSY
210 220 230 240 250
AGKNGHAYRS IGKVLIDRGE VKKEDMSMQA IRHWGETHSE AEVRELLEQN
260 270 280 290 300
PSFVFFKPQS FAPVKGASAV PLVGRASVAS DRSIIPPGTT LLAEVPLLDN
310 320 330 340 350
NGKFNGQYEL RLMVALDVGG AIKGQHFDIY QGIGPEAGHR AGWYNHYGRV
360
WVLKTAPGAG NVFSG
Length:365
Mass (Da):40,411
Last modified:July 19, 2005 - v1
Checksum:i5ECBB92C1E8D5969
GO

Sequence cautioni

The sequence AAB40463 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti346H → N in AAC45723 (PubMed:9287002).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32224 Genomic DNA. Translation: AAC45723.1.
U29581 Genomic DNA. Translation: AAB40463.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75855.1.
AP009048 Genomic DNA. Translation: BAE76885.1.
PIRiA65064.
RefSeqiNP_417293.1. NC_000913.3.
WP_000678646.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75855; AAC75855; b2813.
BAE76885; BAE76885; BAE76885.
GeneIDi944964.
KEGGiecj:JW2784.
eco:b2813.
PATRICi32121044. VBIEscCol129921_2914.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32224 Genomic DNA. Translation: AAC45723.1.
U29581 Genomic DNA. Translation: AAB40463.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75855.1.
AP009048 Genomic DNA. Translation: BAE76885.1.
PIRiA65064.
RefSeqiNP_417293.1. NC_000913.3.
WP_000678646.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AE0X-ray2.00X22-365[»]
2GAEX-ray2.50A22-365[»]
2PI8X-ray2.25A/B/C/D22-365[»]
2PICX-ray2.25A22-365[»]
2PJJX-ray2.46A22-365[»]
ProteinModelPortaliP0A935.
SMRiP0A935.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261126. 187 interactors.
DIPiDIP-51238N.
IntActiP0A935. 1 interactor.
STRINGi511145.b2813.

Protein family/group databases

CAZyiGH102. Glycoside Hydrolase Family 102.

Proteomic databases

PaxDbiP0A935.
PRIDEiP0A935.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75855; AAC75855; b2813.
BAE76885; BAE76885; BAE76885.
GeneIDi944964.
KEGGiecj:JW2784.
eco:b2813.
PATRICi32121044. VBIEscCol129921_2914.

Organism-specific databases

EchoBASEiEB2894.
EcoGeneiEG13085. mltA.

Phylogenomic databases

eggNOGiENOG4105CA3. Bacteria.
COG2821. LUCA.
HOGENOMiHOG000278098.
InParanoidiP0A935.
KOiK08304.
OMAiKREDMSM.
PhylomeDBiP0A935.

Enzyme and pathway databases

BioCyciEcoCyc:G7457-MONOMER.
ECOL316407:JW2784-MONOMER.
MetaCyc:G7457-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A935.
PROiP0A935.

Family and domain databases

Gene3Di2.40.40.10. 2 hits.
InterProiIPR010611. 3D_dom.
IPR005300. Lytic_transglycosylase_MltA.
IPR009009. RlpA-like_DPBB.
[Graphical view]
PfamiPF06725. 3D. 1 hit.
PF03562. MltA. 1 hit.
[Graphical view]
SMARTiSM00925. MltA. 1 hit.
[Graphical view]
SUPFAMiSSF50685. SSF50685. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMLTA_ECOLI
AccessioniPrimary (citable) accession number: P0A935
Secondary accession number(s): P46885
, P76638, Q2MA21, Q46928
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: November 30, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.